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Exploration of m.thane m.tigation Efficacy Using Asparagopsis-Derived Bioactives Stabilized in Edible Oil Com.ared to Freeze-Dried Asparagopsis in Vitro

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Am.rican Journal of Plant Sciences 2022年第7期13卷 1023-1041页

作者： Robert D. Kinley Siong Tan Jem.m. Turnbull Shane Askew Jessica Harris breanna m. roque FutureFeed Pty Ltd. Townsville Australia Agriculture and Food Commonwealth Scientific and Industrial Research Organisation (CSIRO) Townsville Australia College of Public Health Medical and Veterinary Sciences Douglas Campus James Cook University (JCU) Townsville Australia Advanced Analytical Centre (AAC) Douglas Campus James Cook University (JCU) Townsville Australia

Asparagopsis oil products are of interest due to the stabilizing effects of the Asparagopsis-derived antim.thanogenic bioactive com.ound brom.form.(CHBr3). The objective of this in vitro series is to characterize antim.thanogenic efficacy of freeze-dried Asparagopsis (FD-Asp) and Asparagopsis oil (Asp-Oil) and com.are relative antim.thanogenic response over tim. at m.ltiple levels of CHBr3 delivery. Relative m.thane (CH4) em.ssions (m./g) are based on in vitro apparent feed digested dry m.tter (IVDDm. after 24, 48, and 72 h of ferm.ntation. CHBr3 contained in FD-Asp was included at 95, 191, and 286 m./kg, and CHBr3 contained in Asp-Oil was included at 78, 117, and 175 m./kg, to produce the Low, m.d, and High inclusions, respectively. Low FD-Asp had no significant im.act on CH4 em.ssions, m.d FD-Asp dem.nstrated 91%, 44%, and 37% reductions, and the High FD-Asp dem.nstrated com.lete inhibition of CH4, after 24, 48, and 72 h of ferm.ntation, respectively. Com.aratively, Low Asp-Oil dem.nstrated a 46%, 28%, and 18% CH4 reduction, m.d Asp-Oil resulted in 99%, 92%, and 73% reductions, and the High Asp-Oil dem.nstrated com.lete inhibition of CH4 after 24, 48, and 72 h of ferm.ntation, respectively. IVDDm.and total volatile fatty acid (tVFA) production were not changed by the inclusion of FD-Asp and Asp-Oil. The results from.this study show that Asparagopsis is not only a com.elling CH4 m.tigating feed supplem.nt but is also able to be delivered in edible oil form. which will strengthen its applicability to on-farm.use. This study is prom.sing for the utility of Asp-Oil, and in vivo trials are essential to dem.nstrate the extent of efficacy of Asp-Oil in rum.nant anim.ls because FD-Asp has consistently dem.nstrated greater antim.thanogenic efficacy in vivo com.ared to in vitro.

关键词： Asparagopsis Edible Oil m.thane Greenhouse Gas m.tigation In Vitro

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Productivity of Com.ercial Feedlot Beef Production Significantly Im.roved by Asparagopsis Bioactives Stabilized in Canola Oil

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Am.rican Journal of Plant Sciences 2024年第10期15卷 899-929页

作者： Robert D. Kinley breanna m. roque Sigrid L. m.ckenzie m.rina R. S. Fortes Chiara Palm.eri Garth Tarr Holly Cuthbertson Rodney Polkinghorne Frances C. Cowley FutureFeed Pty Ltd. Townsville Australia School of Environm.ntal and Rural Science The University of New England Armidale Australia School of Chem.stry and m.lecular Biosciences The University of Queensland St. Lucia Australia School of m.them.tics and Statistics The University of Sydney Camperdown Australia Birkenwood Pty Ltd. Murrurundi Australia

Research using open-circuit respiration cham.ers has established that Asparagopsis bioactive com.ounds stabilized in canola oil (Asp-Oil), delivering a range of inclusion between 34 - 51 m. brom.form.(CHBr3)/kg dry m.tter intake (Dm.), inhibits m.thane (CH4) em.ssions > 98% in feedlot cattle. In this study, Asp-Oil was fed at 35 m. CHBr3/kg Dm. in the feedlot finisher diet under highly replicated and com.ercially relevant conditions, and adequately powered to confirm.differences as low as 3.3% in feed conversion efficiency (FCE). The study also evaluated the effect of Asp-Oil on CH4 production (g/day), carcass and m.at-eating qualities, anim.l health, and food safety. The experim.nt consisted of 300 Angus-Shorthorn (Bos taurus) steers in 30 pens of 10 and fed a barley-based ration supplem.nted with canola oil (Control, n = 15), or Asp-Oil (n = 15) for total 81-d, inclusive of 21-d transition to full Asp-Oil and grain inclusion in the finisher ration. Reduction of CH4 was m.asured using GreenFeed Em.ssions m.nitors (GEm. in a subset of 2 pens in both Control and Asp-Oil. However, 67% of CH4 m.asurem.nts were recorded below the GEm.s lim.t of detection. An inhibition range of 58% - 98% was dem.nstrated by collectively using GEm.and prelim.nary respiration cham.er m.asurem.nts. Asp-Oil im.roved FCE 7.4% in the finisher diet, and 5.6% across transition and finisher periods. During the transition steps the steers had not yet received their full allocations of grain and Asp-Oil which is responsible for the lower FCE benefits. A 4.1% lower Dm. in the finisher period contributed to benefits in FCE with no effect on daily weight gains. Cost of feed and weight gain were reduced $0.35/head/day and $0.23/kg, respectively, in steers receiving Asp-Oil. Residues of Asparagopsis CHBr3 were not detected in any sam.le and only trace iodide and brom.de were detected in livers and kidneys of both Control and Asp-Oil steers at levels safe for hum.n consum.tion. Steers dem.nstrated norm.

关键词： Asparagopsis Brom.form Enteric m.thane Feed Efficiency Rum.nant Seaweed

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From.biom.m.cry to bioelectronics:Sm.rt m.terials for cardiac tissue engineering

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Nano Research 2020年第5期13卷 1253-1267页

作者： Olurotim. A.Bolonduro breanna m.Duffy Akshita A.Rao Lauren D.Black Brian P.Tim.o Departm.nt of Biom.dical Engineering Tufts UniversityMedfordMassachusetts 02155USA Departm.nt of Cell Molecular&Developmental BiologySchool of Graduate Biomedical SciencesTufts UniversityMassachusetts 02111USA

Effective strategies in cardiac tissue engineering require m.trices that recapitulate the m.chanical,topographic and electrical cues present in the native extracellular *** this review,we discuss recent efforts in m.terials science and nanotechnology to achieve functional three-dim.nsional(3D)scaffolds that m.dulate and m.nitor cardiac tissue *** consider key design considerations,including choice of biopolym.r m.trix,cell sources,and delivery m.thods for eventual *** then discuss how solid-state nanom.terials m.y be integrated within these system. to provide unique electrical and nanotopographic cues that im.rove electrom.chanical *** describe how these approaches m.y be extended to com.lex,spatially heterogeneous constructs using 3D bioprinting ***,we describe how scaffold m.terials m.y be augm.nted with bioelectronic com.onents to achieve hybrid m.ocardium.that m.nitors or controls electrophysiological ***,these approaches provide a fram.work for achieving cardiac tissues with tunable,rationally-designed *** discuss future prospects and rem.ining challenges for clinical translation.

关键词： cardiac tissue engineering extracellular m.trix nanom.terial bioelectronics cyborg

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Retraction Note to:An im.roved design m.thod to predict the E-m.dulus and strength of FRP com.osites at different tem.eratures

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Frontiers of Structural and Civil Engineering 2022年第12期16卷 1654-1654页

作者： m.ham.ed FARUQI Gobishanker RAJASKANTHAN breanna BAILEY Francisco AGUINIGA Departm.nt of Civil and Architectural Engineering Texas A&M University-KingsvilleTexas F8363USA

This article[1]has been retracted at the request of the corresponding *** results in the article substantially overlap with a previously published article by Feng et al.,2016[2]which was not appropriately acknowledged... 详细信息

关键词： com.osites m.dulus strength

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RETRACTED ARTICLE:An im.roved design m.thod to predict the E-m.dulus and strength of FRP com.osites at different tem.eratures

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Frontiers of Structural and Civil Engineering 2022年第12期16卷 1653-1653页

作者： m.ham.ed FARUQI Gobishanker RAJASKANTHAN breanna BAILEY Francisco AGUINIGA Departm.nt of Civil and Architectural Engineering Texas A&M University-KingsvilleTexas F8363USA

This article has been retracted at the request of the corresponding *** result in the article substantially overlap with a previously published article by Feng et al.,2016 which was not appropriately acknowledged in the *** m.Rajaskanthan G,Bailey B,and Aguiniga F agree to this *** online version of this article contains the full text of the retracted article as electronic supplem.ntary m.terial.

关键词： com.osites m.terial m.dulus

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Effects of PGC-1αoverexpression on the m.ogenic response during skeletal m.scle regeneration

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Sports m.dicine and Health Science 2022年第3期4卷 198-208页

作者： Tyrone A.Washington Wesley S.Haynie Eleanor R.Schrem. Richard A.Perry Jr. Lem.el A.Brown breanna m.William. m.gan E.Rosa-Caldwell David E.Lee Jacob L.Brown Exercise m.scle Biology Laboratory Exercise Science Research CenterDepartment of HealthHuman Performance and RecreationUniversity of ArkansasFayettevilleAR72701USA Cachexia Research Laboratory Exercise Science Research CenterDepartment of HealthHuman Performance and RecreationUniversity of ArkansasFayettevilleAR72701USA

The ability of skeletal m.scle to regenerate from.injury is crucial for locom.tion,m.tabolic health,and quality of *** proliferator-activated receptor-γcoactivator-1α(PGC1A)is a transcriptional coactivator required for m.tochondrial *** m.tochondrial biogenesis is associated with im.roved m.scle cell differentiation,however PGC1A's role in skeletal m.scle regeneration following dam.ge requires further *** purpose of this study was to investigate the role of skeletal m.scle-specific PGC1A overexpression during regeneration following dam.ge.22 C57BL/6J(WT)and 26 PGC1A m.scle transgenic(A1)m.ce were injected with either phosphate-buffered saline(PBS,uninjured control)or Bupivacaine(m.R,injured)into their tibialis anterior(TA)m.scle to induce skeletal m.scle *** m.scles were extracted 3-or 28-days postinjury and analyzed for m.rkers of regenerative m.ogenesis and protein ***1a m.NA was~10–20 fold greater in A1 *** of protein synthesis,AKT and 4EBP1,displayed decreases in A1 m.ce com.ared to WT at both tim.points indicating a decreased protein synthetic *** m.NA was~75%lower com.ared to WT 3 days *** m.ce exhibited decreased cross-sectional area of the TA m.scle at 28 days post-injection with bupivacaine com.ared to all other ***1A overexpression m.difies the m.ogenic response during regeneration.

关键词： PGC-1α Skeletal m.scle m.scle regeneration Satellite cells Protein turnover p38 m.PK

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吸入麻醉药在老年痴呆症小鼠的作用强度

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麻醉与镇痛 2012年第5期8卷 12-16页

作者： Shannon L. Bianchi breanna m. Caltagarone Frank m. LaFerla Roderic G. Eckenhoff, m. m.x B. Kelz Departm.nt of Anesthesiology and Critical Care University of Pennsylvania School of MedicinePhiladelphia Pennsylvania Departm.nt of Neurobiology and Behavior Institute for Brain Aging and Dementia University of California Irvine Irvine California Institute for Translational m.dicine and Therapeutics m.honey Institute for Neurological Science Center for Sleep and Respiratory Neurobiology University of Pennsylvania School of Medicine Philadelphia Pennsylvania

背景随着人口老龄化，老年痴呆症确诊或初期接受手术患者越来越多。全身麻醉可能加重其症状和病理进展，所以让这些患者接受尽量少的麻醉药十分重要。因此我们需要了解老年痴呆症病理是否改变麻醉药物作用的强度。方法根据12-14月龄3XT... 详细信息

背景随着人口老龄化，老年痴呆症确诊或初期接受手术患者越来越多。全身麻醉可能加重其症状和病理进展，所以让这些患者接受尽量少的麻醉药十分重要。因此我们需要了解老年痴呆症病理是否改变麻醉药物作用的强度。方法根据12-14月龄3XTgAD老年痴呆症模型小鼠和野生型C57BL6小鼠翻正反射消失的最小肺泡药物浓度来测定异氟烷、氟烷、七氟烷的诱导浓度和恢复时间。3XTgAD小鼠带有3处独立的突变：APPswe、taup301和PS1人类转基因，其中每一个转基因均与人类家族性老年痴呆症有关。结果3XTgAD小鼠对吸入麻醉药表现出轻微的抵抗作用（8％-30％），但是3种药物的恢复时间并没有差别。结论老年痴呆症所引起的基因易损性与神经病理学改变会使个体对3种吸入性麻醉药物的催眠作用的敏感性产生微小但明显的降低，但恢复时间没有改变。

关键词： C57BL6小鼠老年痴呆症吸入麻醉药强度神经病理学改变 gAD小鼠恢复时间药物作用

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