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Molecular cloning,tissue distribution and the expression of cystine/glutamate exchanger(xCT,SLC7A11) in different tissues during development in broiler chickens

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Animal Nutrition 2020年第1期6卷 107-114页

作者： Janghan Choi Weiqi Li brayden schindell Liju Ni Shangxi Liu Xiaoya Zhao Joshua Gong Martin Nyachoti Chengbo Yang Department of Animal Science University of ManitobaWinnipegMBR3T 2N2Canada Shanghai Lab-Animal Research Center Shanghai201203China Guelph Research and Development Centre Agriculture Agri-Food CanadaGuelphONNIG 5C9Canada

The cystine/glutamate exchanger(xCT,SLC7 A11)is a component of the system X_c amino-acid antiporter that is able to export glutamate and import cysteine into *** xCT amino acid exchanger has received a lot of attention,due to the fact that cysteine is an essential substrate for the synthesis of glutathione(GSH),an endogenous antioxidant in *** objective of this research was to clone the full-length cDNA of chicken xCT,and to investigate the gene expression of xCT in different tissues,including intestinal segments of broiler chickens during *** full-length cDNA of chicken xCT(2,703 bp)was obtained from the jejunum by reverse transcription-PCR and *** tests showed that chicken xCT had 80.4%,80.2%,and 71.2%homology at the nucleotide level with humans,cattle,and rats,***,amino acid sequence analysis showed that chicken xCT protein is 86.4%,79.3%,and 75.6%homologous with humans,cattle,and rats,***,phylogenetic analysis indicated that chicken xCT genes share a closer genetic relationship with humans and cattle,than with *** chicken xCT protein has 12 transmembrane helixes,6 extracellular loops,and 5 intracellular *** mRNA of xCT was detected in all tissues,including intestinal segments,in which the mRNA expression of xCT was significantly higher(P<0.05)within the colon,compared to the jejunum and *** development,a linear pattern of changes regarding the levels of the xCT mRNA was found,indicating that there was an abundance of xCT within the duodenum(P<0.05).Furthermore,there were changes of the xCT mRNA abundance in the colon during development,which displayed linear and cubic patterns(P<0.05).These results indicated that xCT is widely expressed both in intestinal segments,as well as other organs that are not associated with nutrient *** investigation is needed to characterize the functional relevance of xCT activity in oxidative stress and inflammation in th

关键词： Amino acid Broiler chickens Cystine/glutamate transporter Development Gene expression

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