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Genomic regions underlying agronomic traits in linseed(Linum usitatissimum L.) as revealed by association mapping

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Journal of Integrative Plant Biology 2014年第1期56卷 75-87页

作者： braulio J.Soto-Cerda Scott Duguid Helen Booker Gordon Rowland Axel Diederichsen Sylvie Cloutier Department of Plant Science University of Manitoba 66 Dafoe Road Cereal Research Center Agriculture and Agri-Food Canada 195 Dafoe Road Morden Research Station Agriculture and Agri-Food Canada 101 Route 100 Unit 100 Morden Department of Plant Sciences College of Agriculture and Bioresources University of Saskatchewan 51 Campus Drive Plant Gene Resources of Canada Agriculture and Agri-Food Canada 107 Science Place

The extreme climate of the Canadian Prairies poses a major chal enge to improve yield. Although it is possible to breed for yield per se, focusing on yield-related traits could be advantageous because of their simpler genetic architecture. The Canadian flax core col ection of 390 accessions was genotyped with 464 simple sequence repeat markers, and phenotypic data for nine agronomic traits including yield, bol s per area, 1,000 seed weight, seeds per bol , start of flowering, end of flowering, plant height, plant branching, and lodging col ected from up to eight environments was used for association mapping. Based on a mixed model （principal component analysis （PCA） t kinship matrix （K））, 12 significant marker-trait associations for six agronomic traits were identi-fied. Most of the associations were stable across environments as revealed by multivariate analyses. Statistical simulation for five markers associated with 1000 seed weight indicated that the favorable al eles have additive effects. None of the modern cultivars carried the five favorable al eles and the maximum number of four observed in any accessions was mostly in＆amp;nbsp;breeding lines. Our results confirmed the complex genetic architecture of yield-related traits and the inherent difficulties associated with their identification while il ustrating the potential for improvement through marker-assisted selection.

关键词： Linum usitatissimum marker-assisted selection

Proliferative vitreoretinopathy and its relationship with inflammatory serum biomarkers

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International Journal of Ophthalmology(English edition) 2024年第9期17卷 1659-1664页

作者： Victor Alejandro Martinez Pacheco Sergio Rojas Juarez braulio Hernan Velasco Sepulveda Abel Ramirez Estudillo Hospital de Nuestra Señora de la Luz Retina and Vitreous DepartmentMexico City 06030Mexico Instituto de Oftlamología FAP Conde de Valenciana Mexico City 06080Mexico

AIM:To analyze if a relationship between levels ofinflammatory serum biomarkers and severity of primaryproliferative vitreoretinopathy(PVR)***:A retrospective case-control *** adult patients with rhegmatogenous retinaldetachment and primary PVR were included in the *** the control group,healthy adults who underwentcataract surgery were *** grade of PVR wasclassified according to the Retinal Society *** samples were obtained before surgery,and processed in MYTHIC *** of interest wereneutrophil-to-lymphocyte ratio(NLR),platelet-to-lymphocyteratio(PLR),and lymphocyte-to-monocyte ratio(LMR),thetime between the decrease in visual acuity and surgery,PVRgrade,type of surgery,final best corrected visual acuity,andrate of ***:Totally 240 patients were included,120 ineach group,79(65.8%)and 56(46.7%)were male in thePVR and control group,*** A had greaterlevels of monocytes(0.28±0.18 vs 0.12±0.32,P=0.002),neutrophils(4.59±1.51 vs 3.92±1.27,P=0.006),and LMR(9.32±4.42 vs 7.43±3.90,P=0.01).PVR B had a greatermonocyte count(0.30±0.13 vs 0.12±0.32,P=0.001),andPVR C demonstrated higher levels in monocytes(0.27±0.12vs 0.12±0.32,P=0.004),neutrophils(4.39±1.13 vs3.92±1.27,P=0.004),and LMR(9.63±3.24 vs 7.43±3.90,P=0.002)compared to control,*** LMR cut-offvalue of 9.38 predicted PVR with a sensibility of 54.2%andspecificity of 77.5%and NLR cut-off of 1.70 predicted PVRwith a sensibility of 62%and specificity of 54.2%.CONCLUSION:Patients with primary PVR demonstrategreater neutrophil,monocyte,and LMR levels than thecontrol ***-off values obtained from ratios could beuseful in a clinical setting when no posterior view of thefundus is possible due to media opacity.

关键词： biomarkers proliferative vitreoretinopathy retinal detachment inflammation serum

A modular microfluidic platform to study how fluid shear stress alters estrogen receptor phenotype in ER+breast cancer cells

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Microsystems & Nanoengineering 2024年第1期10卷 287-299页

作者： braulio Andrés Ortega Quesada Jonathan Cuccia Rachael Coates Blake Nassar Ethan Littlefield Elizabeth C.Martin Adam T.Melvin Cain Department of Chemical Engineering Louisiana State UniversityBaton RougeLA 70803USA Department of Chemical and Biological Engineering Clemson UniversityClemsonSC 29634USA Biological and Agricultural Engineering Louisiana State UniversityBaton RougeLA 70803USA Department Medicine Section Hematology and Medical OncologyTulane UniversityNew OrleansLA 70118USA

Metastatic breast cancer leads to poor prognoses and worse outcomes in patients due to its invasive behavior and poor response to *** is still unclear what biophysical and biochemical factors drive this more aggressive phenotype in metastatic cancer;however recent studies have suggested that exposure to fluid shear stress in the vasculature could cause *** this study a modular microfluidic platform capable of mimicking the magnitude of fluid shear stress(FSS)found in human vasculature was designed and *** device provides a platform to evaluate the effects of FSS on MCF-7 cell line,an estrogen receptor positive(ER+)breast cancer cell line,during circulation in the *** of the effects of FSS on MCF-7 cells was carried out utilizing two approaches:single cell analysis and bulk *** single cell analysis,cells were trapped in a microarray after exiting the serpentine channel and followed by immunostaining on the device(on-chip).Bulk analysis was performed after cells were collected in a microtube at the outlet of the microfluidic serpentine channel for western blotting(off-chip).It was found that cells exposed to an FSS magnitude of 10 dyn/cm^(2) with a residence time of 60 s enhanced expression of the proliferation marker Ki67 in the MCF-7 cell line at a single cell *** understand possible mechanisms for enhanced Ki67 expression,on-chip and off-chip analyses were performed for pro-growth and survival pathways ERK,AKT,and JAK/*** demonstrated that after shearing the cells phosphorylation of p-AKT,p-mTOR,and p-STAT3 were ***,there was no change in p-ERK1/*** is a mediator of ER rapid signaling,analysis of phosphorylated ERαwas carried out and no significant differences between sheared and non-sheared populations were *** together these results demonstrate that FSS can increase phosphorylation of proteins associated with a more aggressive phenotype in circulating cancer *** findin

关键词： fluid breast estrogen

Tracking leaf area index and coefficient of light extinction over the harvesting cycle of black wattle

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Journal of Forestry Research 2016年第6期27卷 1211-1217页

作者： Aiexandre Behling Carlos Roberto Sanquetta Ana Paula Dalla Corte Sylvio Pellico Netto Aurelio Lourenco Rodrigues braulio Otomar Caron Augusto Arlindo Simon Federal University of Parana 900Lothario Meissner AvCuritibaParana 80210-170Brazil Federal University of Santa Maria 1000 Roraima AvSanta MariaRio Grande do SulBrazil TANAC SA 199 Torbjom Weibull StMontenegroRio Grande do SulBrazil

The amount of photosynthetic radiation inter- cepted by a crop is a function of the incident solar radiation on the plants, the leaf area index （LAI）, and the light extinction coefficient （k）. We quantified LAI and k in stands of black wattle （Acacia mearnsii De Wild.） over a 7-year growth cycle at two locations in the state of Rio Grande do Sul, Brazil. Our study was conducted in commercial stands in agroecological regions with high densities of black wattle plantations. LAI was calculated as the ratio between the leaf area of a tree and its planting space, and k was derived from Beer＇s law. LAI depends on the planting site and stand age. Between the two sites, the LAI was similar over time, the amount of variation differed. Values of k depended only on stand age, with the highest average observed for stands up to 5 years old. The trend of k during the plantation cycle was inversely proportional to LAI and was correlated with LAI, leaf area, leaf dry mass, canopy volume, height, branches dry mass, total dry mass, and crown diameter.

关键词： Leaf area - Light attenuation Tree canopy Biomass Photosynthetic radiation intercepted

Pyrolysis-induced shrinking of threedimensional structures fabricated by twophoton polymerization:experiment and theoretical model

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Microsystems & Nanoengineering 2019年第1期5卷 303-315页

作者： braulio Cardenas-Benitez Carsten Eschenbaum Dario Mager Jan G.Korvink Marc JMadou Uli Lemmer Israel De Leon Sergio O.Martinez-Chapa School of Engineering and Sciences Tecnologico de MonterreyAv.Eugenio Garza Sada 2501 Sur64849 MonterreyNLMexico Light Technology Institute Karlsruhe Institute of Technology(KIT)Engesserstraße 1376131 KarlsruheGermany Institute of Microstructure Technology Karlsruhe Institute of Technology(KIT)Hermann-von-Helmholtz-Platz 176344 Eggenstein-LeopoldshafenGermany Department of Mechanical and Aerospace Engineering University of CaliforniaIrvine4200 Engineering GatewayIrvineCA 92697USA

The introduction of two-photon polymerization(TPP)into the area of Carbon Micro Electromechanical Systems(CMEMS)has enabled the fabrication of three-dimensional glassy carbon nanostructures with geometries previously unattainable through conventional UV *** of TPP structures conveys a characteristic reduction of feature size—one that should be properly estimated in order to produce carbon microdevices with *** this work,we studied the volumetric shrinkage of TPP-derived microwires upon pyrolysis at 900℃.Through this process,photoresist microwires thermally decompose and shrink by as much as 75%,resulting in glassy carbon nanowires with linewidths between 300 and 550 *** after the thermal decomposition induced by the pyrolysis step,the linewidth of the carbon nanowires was found to be dependent on the TPP exposure *** have also found that the thermal stress induced during the pyrolysis step not only results in axial elongation of the nanowires,but also in buckling in the case of slender carbon nanowires(for aspect ratios greater than 30).Furthermore,we show that the calculated residual mass fraction that remains after pyrolysis depends on the characteristic dimensions of the photoresist microwires,a trend that is consistent with several works found in the *** phenomenon is explained through a semi-empirical model that estimates the feature size of the carbon structures,serving as a simple guideline for shrinkage evaluation in other designs.

关键词： lithography pyrolysis structures

ODEBRECHT公司不当行为危机管理研究

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ODEBRECHT公司不当行为危机管理研究

作者： braulio Ulloa Quesada 上海外国语大学

学位级别：硕士

本研究的背景是一家总部位于巴西的建筑公司奥德布雷希特（Odebrecht）的危机管理。奥德布雷希特公司在拉丁美洲拥有大量业务,该公司在2014年出现了一个巨大的腐败丑闻,其中涉及诸多地方政府。研究重点在于证明危机与公司管理层的关联,... 详细信息

本研究的背景是一家总部位于巴西的建筑公司奥德布雷希特（Odebrecht）的危机管理。奥德布雷希特公司在拉丁美洲拥有大量业务,该公司在2014年出现了一个巨大的腐败丑闻,其中涉及诸多地方政府。研究重点在于证明危机与公司管理层的关联,以及不同层级和不同支持领域能如何解决危机,并以奥德布雷希特公司的案例证明危机管理不只关乎公关。此外,本文还试图确定该公司危机产生的主要影响及产生的主要原因。本文旨在表明需要正确分析危机,包括其影响和问题,因为若想作出更好的评估,必须先确定原因,从而减轻危机影响,避免今后发生类似危机。此外,本文还试图证明在危机中对管理层问责是有用且必要的。最后,本研究旨在为其他面临公关危机的公司提供参考,他们可以将这些发现运用到类似情况中,克服挑战。因此,本文遵循定性类型研究,重点研究危机管理、情境危机传播、形象修复、利益相关者管理和道德领导力等管理理论。奥德布雷希特的外部和内部环境为公司的非伦理领导层和不良的公司治理提供了证据。理解这些因素有助于基于管理派生理论提出建议,尝试解决这些问题,同时管理和减轻危机后的影响。本文为公司内部不同层级的管理层提出了实施计划,以证明管理层有必要参与、支持危机管理,并得出总体结论,提出各自的局限性和发现的困难,以及进一步的研究方向。

关键词：危机管理非伦理领导公司治理

Effect of different anesthetic mixtures——ketamine-xylazine, ketamine-acepromazine and tiletamine-zolazepam——on the physiological and blood biochemistry parameters of male rhesus monkeys(Macaca mulatta) at different ages

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Animal Models and Experimental Medicine 2019年第2期2卷 83-97页

作者： braulio Hernández‐Godínez Herlinda Bonilla Jaime Adrián Poblano Marcela Arteaga‐Silva Ameyalli Medina Hernández Armando Contreras‐Uribe Alejandra Ibanez-Contreras Posgrado en Ciencias Biológicas y de la Salud Unidad Iztapalapa Universidad Autónoma Metropolitana Ciudad de México México Investigación Biomédica Aplicada (INBIOMA) S.A.S. de C.V. Ciudad de México México Alimentos y Camas Zoosanitarias (ACAZOO) S. de R.L. Ciudad de México México Departamento Biología de la Reproducción Unidad Iztapalapa Universidad Autónoma Metropolitana Ciudad de México México Laboratorio de Neurofisiología Cognoscitiva Instituto Nacional de Rehabilitación Ciudad de México México

Background: Anesthetic agents are commonly utilized in the handling of non‐human primates for prevent the stress caused in physical exploration or physical restrain. For this reason, the objective of this work was to describe the effect of age and dissociative anesthetics (ketamine and tiletamine), and their combinations with acepromazine, xylazine and zolazepam, on the physiological and blood biochemical parameters in Macaca mulatta. Methods: Eighty male Macaca mulatta were divided into four experimental groups depending on the anesthetic mixture applied. Each group of 20 males was divided into five sub‐groups according to age. Physiological parameters were recorded every five minutes during a 30‐minute period. A blood sample was drawn to analyze blood biochemistry. Results: Statistical analyses revealed significant differences in the physiological parameters between the ketamine‐acepromazine and ketamine‐xylazine groups compared to the control group. The analysis of blood biochemistry found significant differences by age and by anesthetic mixture among all groups. Conclusion: These findings contribute to standardizing this animal model in biological research.

关键词： acepromazine anesthesia blood biochemistry ketamine Macaca mulatta physiologic parameters tiletamine xylazine zolazepam

Product decomposition strategy for optimization of supply chain planning

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Frontiers of Engineering Management 2018年第4期5卷 466-478页

作者： braulio BRUNAUD Maria Paz OCHOA Ignacio E.GROSSMANN Department of Chemical Engineering Carnegie Mellon University

Optimization of large-scale supply chain planning models requires the application of decomposition strategies to reduce the computational expense. Two major options are to use either spatial or temporal Lagrangean decomposition. In this paper, to further reduce the computational expense a novel decomposition scheme by products is presented. The decomposition is based on a reformulation of knapsack constraints in the problem. The new approach allows for simultaneous decomposition by products and by time periods, enabling the generation of a large number of subproblems, that can be solved by using parallel computing. The case study shows that the proposed product decomposition exhibits similar performance as the temporal decomposition, and that selecting different orders of products and aggregating the linking constraints can improve the efficiency of the algorithm.

关键词： supply chain planning Lagrangean decomposition mixed-integer programming

Protective effects of cyclosporine and its analog NIM-811 in a murine model of hepatic ischemia-reperfusion injury

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Liver Research 2024年第1期8卷 46-53页

作者： Joshua Hefler Rena Pawlick braulio A.Marfil-Garza Aducio Thiesen Nerea Cuesta-Gomez Sanaz Hatami Darren H.Freed Constantine Karvellas David L.Bigam A.M.James Shapiro Department of Surgery Faculty of Medicine&DentistryUniversity of AlbertaEdmontonABCanada Department of Medicine Faculty of Medicine&DentistryUniversity of AlbertaEdmontonABCanada CHRISTUS-LatAm Hub Excellence&Innovation Center MonterreyMexico Alberta Diabetes Institute University of AlbertaEdmontonABCanada Clinical Islet Transplant Program University of AlbertaEdmontonABCanada Department of Laboratory Medicine&Pathology Faculty of Medicine&DentistryUniversity of AlbertaEdmontonABCanada

Background and aim:The liver is susceptible to ischemia-reperfusion injury(IRI)during hepatic surgery,when the vessels are compressed to control bleeding,or liver transplantation,when there is an obligate period of *** hallmark of IRI comprises mitochondrial dysfunction,which generates reactive oxygen species,and cell death through necrosis or ***(CsA),which is a well-known immunosuppressive agent that inhibits calcineurin,has the additional effect of inhibiting the mito-chondrial permeability transition pore(mPTP),thereby,preventing mitochondrial swelling and ***-811,which is the nonimmunosuppressive analog of CsA,has a similar effect on the *** this study,we tested the effect of both agents on mitigating warm hepatic IRI in a murine *** and methods:Before ischemic insult,the mice were administered with intraperitoneal normal saline(control);CsA at 2.5,10,or 25 mg/kg;or NIM-811 at 10 mg/***,the mice were subjected to partial warm hepatic ischemia by selective pedicle clamping for 60 min,followed by 6 h of recovery after *** alanine transaminase(ALT)was measured,and the liver tissue was examined histologically for the presence of apoptosis and the levels of inflammatory ***:Compared with the control mice,the mice treated with 10 and 25 mg/kg of CsA and NIM-811 had significantly lower ALT levels(P<0.001,0.007,and 0.031,respectively).Moreover,the liver tissue showed reduced histological injury scores after treatment with CsA at 2.5,10,and 25 mg/kg and NIM-811(P=0.041,<0.001,0.003,and 0.043,respectively)and significant decrease in apoptosis after treatment with CsA at all doses(P=0.012,0.007,and<0.001,respectively).Levels of the pro-inflammatory cyto-kines,particularly interleukin(IL)-1β,IL-2,IL-4,IL-10,and keratinocyte chemoattractant/human growth-regulated oncogene significantly decreased in the mice treated with the highest dose of CsA(25 mg/kg)than those in the control ***

关键词： Cyclosporine(CsA) Cyclosporine analogue Liver surgery Ischemia-reperfusion injury(IRI) Animal model NIM-811