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On the cell biology of pit cells,the liver-specific NK cells

World Journal of Gastroenterology 2000年第1期6卷 1-11页

作者： Luo DZ Vermijlen D Ahishali B Triantis V Plakoutsi G braet f Vanderkerken K Wisse E Laboratory for Cell Biology and Histology Free University of Brussels(VUB)Brussels-JetteBelgium Department of Pathology Guangxi Medical University NanningChina Laboratory for Cell Biology and Histology Free University of Brussels(VUB)Brussels-JetteBelgium Department of Hematology and Immunology Free University of Brussels(VUB)Brussels-JetteBelgium

INTRODUCTION Natural killer (NK) cells are functionally defined by their ability to kill certain tumor cells and virus-infected cells without prior

关键词： Subject headings pit cells hepatic natural killer cells large granular lymphocytes

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Structural and functional aspects of the liver and liver sinusoidal cells in relation to colon carcinoma metastasis

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World Journal of Gastroenterology 2005年第33期11卷 5095-5102页

作者： Katrien Vekemans filip braet Centre of Experimental Surgery and Anaesthesia Abdominal Transplant Surgery Department Catholic University of Leuven (KUL)Herestraat 49 3000 Leuven Belgium Australian Key Centre for Microscopy and Microanalysis Electron Microscopy Unit The University of SydneySydney 2006NSWAustralia

Nowadays, liver metastasis remains difficult to cure. When tumor cells escape and arrive in the liver sinusoids, they encounter the local defense mechanism specific to the liver. The sinusoidal cells have been widely described in physiologic conditions and in relation to metastasis during the past 30 years. This paper provides an “overview” of how these cells function in health and in diseases such as

关键词： Metastasis Liver Colon carcinoma Sinusoidalcells Kupffer cells Liver sinusoidal endothelial cells Hepatic NK cells

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Quality evaluation of synthetic quorum sensing peptides used in R&D

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Journal of Pharmaceutical Analysis 2015年第3期5卷 169-181页

作者： frederick Verbeke Evelien Wynendaele Sarah braet Matthias D'Hondt Bart De Spiegeleer Drug Quality and Registration(DruQuaR) Group Faculty of Pharmaceutical SciencesGhent University

Peptides are becoming an important class of molecules in the pharmaceutical field. Closely related peptide-impurities in peptides are inherent to the synthesis approach and have demonstrated to potentially mask biomedical experimental results. Quorum sensing peptides are attracting high interest in R＆D and therefore a representative set of quorum sensing peptides, with a requested purity of at least 95.0%, was evaluated for their purity and nature of related impurities. In-house quality control （QC） revealed a large discrepancy between the purity levels as stated on the supplier＇s certificate of analysis and our QC results. By using our QC analysis flowchart, we demonstrated that only 44.0% of the peptides met the required purity. The main compound of one sample was even found to have a different structure compared to the desired peptide. We also found that the majority of the related impurities were lacking amino acid（s） in the desired peptide sequence. Relying on the certificates of analysis as provided by the supplier might have serious consequences for peptide research, and peptide-researchers should implement and maintain a thorough in-house QC.

关键词： Quorum sensing peptides Quality Impurity profiling

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Unlocking the ultrastructure of colorectal cancer cells in vitro using selective staining

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World Journal of Gastroenterology 2010年第22期16卷 2743-2753页

作者： Joanna M Biazik Kristina A Jahn filip braet Australian Key Centre for Microscopy and Microanalysis Madsen Building F09 The University of Sydney

AIM: To characterise differences between three widely used colorectal cancer cell lines using ultrastructural selective staining for glycogen to determine variation in metastatic properties. METHODS: Transmission electron microscopy was used in this investigation to help identify intracellular structures and morphological features which are precursors of tumor invasion. In addition to morphological markers, we used selective staining of glycogen as a marker for neoplastic cellular proliferation and determined whether levels of glycogen change between the three different cell lines. RESULTS: Ultrastructural analysis revealed morphological differences between the cell lines, as well as differentiation into two sub-populations within each cell line. Caco-2 cells contained large glycogen deposits as well as showing the most obvious morphological changes between the two sub-populations. SW480 cells also contained large glycogen stores as well as deep cellular protrusions when grown on porous filter membranes. HT-29 cells had trace amounts of glycogen stores with few cellular projections into the filter pores and no tight junction ***: Morphology indicative of metastatic properties coincided with larger glycogen deposits, providing strong evidence for the use of selective staining to determine the neoplastic properties of cells.

关键词： Cancer Colorectal Electron microscopy Glycogen Potassium ferrocyanide

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