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Bioactivity of silk fibroin peptides on vascular endothelial cells

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Frontiers of Materials Science 2024年第1期18卷 85-94页

作者： Mengnan Dai Meng Li Peixuan Li boyu zhang Jianmei Xu Jiannan Wang National Engineering Laboratory for Modern Silk College of Textile and Clothing EngineeringSoochow UniversityNo.199 Ren-ai RoadSuzhou Industrial ParkSuzhou 215123China

To determine the contribution of non-repetitive domains to the bioactivity of the heavy chain in silk fibroin(SF)macromolecules,a gene motif f(1)encoding this fragment and its multimers(f(4)and f(8))were biosynthesized from Escherichia coli BL21.Based on the positive application potential of SF materials for the vascular tissue engineering,this study focused on examining the active response of these polypeptides to vascular endothelial cells.Biosynthetic polypeptides F(1),F(4),and F(8)were separately grafted onto the surfaces of bioinert polyethylene terephthalate(PET)films,resulting in remarkable improvements in the spread and proliferation of human umbilical vein endothelial cells(HUVECs).Using the same grafting dose,the activity of cells on polypeptide-modified PET films enhanced with the increase of the molecular weight of those grafted polypeptides from F(1)to F(8).Meanwhile,the growth of cells on the surface of the alkaline-treated PET film was improved,indicating that the hydrophilicity of the surface material had influence on the growth of HUVECs.Moreover,on surfaces with the same water contact angle,the spread and proliferation activity of cells on PET films were significantly lower than those on polypeptide-modified PET films.

关键词： silk fibroin heavy chain non-repetitive fragment cytoactive endothelial cell

Dendrimer-induced synthesis of porous organosilica capsules for enzyme encapsulation

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Frontiers of Chemical Science and Engineering 2024年第4期18卷 41-48页

作者： Ziyi Chu boyu zhang Zhenhua Wu Jiaxu zhang Yiran Cheng Xueying Wang Jiafu Shi Zhongyi Jiang School of Environmental Science&Engineering Tianjin UniversityTianjin 300072China Collaborative Innovation Center of Chemical Science and Engineering Tianjin 300072China State Key Laboratory of Biochemical Engineering Institute of Process EngineeringChinese Academy of SciencesBeijing 100190China Key Laboratory for Green Chemical Technology of Ministry of Education School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China

Organic matter-induced mineralization is a green and versatile method for synthesizing hybrid nanostructured materials,where the material properties are mainly influenced by the species of natural biomolecules,linear synthetic polymer,or small molecules,limiting their diversity.Herein,we adopted dendrimer poly(amidoamine)(PAMAM)as the inducer to synthesize organosilica-PAMAM network(OSPN)capsules for mannose isomerase(MIase)encapsulation based on a hard-templating method.The structure of OSPN capsules can be precisely regulated by adjusting the molecular weight and concentration of PAMAM,thereby demonstrating a substantial impact on the kinetic behavior of the MIase@OSPN system.The MIase@OSPN system was used for catalytic production of mannose from Dfructose.A mannose yield of 22.24% was obtained,which is higher than that of MIase in organosilica network capsules and similar to that of the free enzyme.The overall catalytic efficiency(kcat/Km)of the MIase@OSPN system for the substrate D-fructose was up to 0.556 s^(-1)·mmol^(-1)·L.Meanwhile,the MIase@OSPN system showed excellent stability and recyclability,maintaining more than 50% of the yield even after 12 cycles.

关键词： enzyme immobilization enzyme catalysis organosilica networks capsules sugar biosynthesis

A modified model of Lundberg-Palmgren rolling contact fatigue formula considering the effects of surface treatments

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Friction 2023年第10期11卷 1922-1938页

作者： boyu zhang Huaiju LIU Peitang WEI Mario GUAGLIANO Shengwen HOU State Key Laboratory of Mechanical Transmissions Chongqing UniversityChongqing 400044China Dept.Mechanical Engineering Politecnico di MilanoMilano 20133Italy Shaanxi Fast Auto Drive Refco Group Ltd. Xi’an 710119China

The Lundberg–Palmgren(L–P)fatigue life formula,as a statistical fatigue theory,has been widely used in the industry.However,its direct applicability is limited to the components treated by surface strengthening technologies.Rolling contact fatigue tests and surface integrity measurements of American Iron and Steel Institute(AISI)9310 rollers with several surface treatments were performed to address this issue.Based on these results,a modified L–P fatigue model was proposed,enabling the consideration of surface modification including surface roughness,residual stress,and hardening introduced by different surface treatments.Compared with the original L–P fatigue formula,its results are more accurate for surface strengthened specimens.Furthermore,this method can assess the contact fatigue life of gears treated by surface strengthening techniques.

关键词： Lundberg-Palmgren(L-P)fatigue life formula surface strengthening techniques surface integrity contact fatigue life assessment

A Generalized Polymer Precursor Ink Design for 3D Printing of Functional Metal Oxides

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Nano-Micro Letters 2023年第10期15卷 433-448页

作者： Hehao Chen Jizhe Wang Siying Peng Dongna Liu Wei Yan Xinggang Shang boyu zhang Yuan Yao Yue Hui Nanjia Zhou Key Laboratory of 3D Micro/Nano Fabrication and Characterization of Zhejiang Province School of Engineering and Research Center for Industries of the FutureWestlake UniversityHangzhou 310030People’s Republic of China Institute of Advanced Technology Westlake Institute for Advanced StudyHangzhou 310024People’s Republic of China School of Materials Science and Engineering Zhejiang UniversityHangzhou 310027People’s Republic of China School of Chemical Engineering and Advanced Materials the University of AdelaideAdelaide 5005Australia

Three-dimensional-structured metal oxides have myriad applications for optoelectronic devices.Comparing to conventional lithography-based manufacturing methods which face significant challenges for 3D device architectures,additive manufacturing approaches such as direct ink writing offer convenient,on-demand manufacturing of 3D oxides with high resolutions down to sub-micrometer scales.However,the lack of a universal ink design strategy greatly limits the choices of printable oxides.Here,a universal,facile synthetic strategy is developed for direct ink writable polymer precursor inks based on metal-polymer coordination effect.Specifically,polyethyleneimine functionalized by ethylenediaminetetraacetic acid is employed as the polymer matrix for adsorbing targeted metal ions.Next,glucose is introduced as a crosslinker for endowing the polymer precursor inks with a thermosetting property required for 3D printing via the Maillard reaction.For demonstrations,binary(i.e.,ZnO,CuO,In_(2)O_(3),Ga_(2)O_(3),TiO_(2),and Y_(2)O_(3)) and ternary metal oxides(i.e.,BaTiO_(3) and SrTiO_(3)) are printed into 3D architectures with sub-micrometer resolution by extruding the inks through ultrafine nozzles.Upon thermal crosslinking and pyrolysis,the 3D microarchitectures with woodpile geometries exhibit strong light-matter coupling in the mid-infrared region.The design strategy for printable inks opens a new pathway toward 3D-printed optoelectronic devices based on functional oxides.

关键词： 3D printing Maillard reaction Polymer-assisted deposition Metal oxide Photonic crystal

Multimaterial Embedded 3D Printing of Composite Reinforced Soft Actuators

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Research 2023年第4期2023卷 727-737页

作者： Zhenhua Wang boyu zhang Qu He Hao Chen Jizhe Wang Yuan Yao Nanjia Zhou Weicheng Cui Key Laboratory of Coastal Environment and Resources of Zhejiang Province School of EngineeringWestlake UniversityHangzhouZhejiang ProvinceChina Institute of Advanced Technology Westlake Institute for Advanced StudyHangzhouZhejiang ProvinceChina Research Center for Industries of the Future and Key Laboratory of 3D Micro/Nano Fabrication and Characterization of Zhejiang ProvinceSchool of EngineeringWestlake UniversityHangzhouZhejiang ProvinceChina

Soft pneumatic actuators(SPAs)have attracted enormous attention in the growing field of robotics.Among different SPAs,composite reinforced actuators(CRAs)are widely used because of their simple structure and high controllability.However,multistep molding,a time-consuming method,is still the predominant fabrication method.Here,we propose a multimaterial embedded printing method(ME3P)to fabricate CRAs.In comparison with other 3-dimensional printing methods,our method improves fabrication flexibility greatly.Via the design and fabrication of the reinforced composites’patterns and different geometries of the soft body,we demonstrate actuators with programmable responses(elongation,contraction,twisting,bending,and helical and omnidirectional bending).Finite element analysis is employed for the prediction of pneumatic responses and the inverse design of actuators based on specific actuation needs.Lastly,we use tube-crawling robots as a model system to demonstrate our ability to fabricate complex soft robots for practical applications.This work demonstrates the versatility of ME3P for the future manufacturing of CRA-based soft robots.

关键词： composites composite printing

从社会语境看非遗的保护与发展——以肉连响为例

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黄钟（武汉音乐学院学报） 2024年第1期 39-47,167页

作者：张伯瑜香港中文大学(深圳)音乐学院中央音乐学院北京100031

湖北省利川市的肉连响入选国家级第二批(2008年)“非物质文化遗产代表作”。表演时,表演者拍打自己的身体,发出阵阵响声。这种起源于为乞讨而表演的“泥神道”在今天的社会语境下失去了原本的意义,成为了土家族文化的代表,目前也是锻炼... 详细信息

湖北省利川市的肉连响入选国家级第二批(2008年)“非物质文化遗产代表作”。表演时,表演者拍打自己的身体,发出阵阵响声。这种起源于为乞讨而表演的“泥神道”在今天的社会语境下失去了原本的意义,成为了土家族文化的代表,目前也是锻炼身体的手段和新创舞蹈作品的素材。该文通过来自田野的实际感受和相关研究文献的研读,思考“肉连响”及其类似的非遗音乐项目的保护与发展问题。作者认为,对于肉连响及其类似的非遗项目来说,保留原属语境的“完整保护”在今天不可能实现,变化与发展以使其适应今天的生活是必然之举,而这类非遗项目在原属语境中的发展程度(含“舞台化”“审美性”以及艺术性上的“完整性”)是我们思考非遗项目在今天的新语境中怎样保护和怎样发展的参考依据。非遗项目的形式与其原属语境和新语境之间变化的关系决定着非遗项目的保护与发展问题,也决定着非遗项目的功能转换。

关键词：肉连响土家族非遗刘守红舞台化

Tunneling magnetoresistance materials and devices for neuromorphic computing

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Materials Futures 2023年第3期2卷 90-113页

作者： Yuxuan Yao Houyi Cheng boyu zhang Jialiang Yin Daoqian Zhu Wenlong Cai Sai Li Weisheng Zhao Fert Beijing Institute MIIT Key Laboratory of SpintronicsSchool of Integrated Circuit Science and EngineeringBeihang UniversityBeijing 100191People’s Republic of China Hefei Innovation Research Institute Anhui High Reliability Chips Engineering LaboratoryBeihang UniversityHefei 230013People’s Republic of China Truth Equipment Corporation TRECHefei 230013People’s Republic of China Beihang-Geortek Joint Microelectronics Institute Qingdao Research InstituteBeihang UniversityQingdao 266061People’s Republic of China

Artificial intelligence has become indispensable in modern life,but its energy consumption has become a significant concern due to its huge storage and computational demands.Artificial intelligence algorithms are mainly based on deep learning algorithms,relying on the backpropagation of convolutional neural networks or binary neural networks.While these algorithms aim to simulate the learning process of the human brain,their low bio-fidelity and the separation of storage and computing units lead to significant energy consumption.The human brain is a remarkable computing machine with extraordinary capabilities for recognizing and processing complex information while consuming very low power.Tunneling magnetoresistance(TMR)-based devices,namely magnetic tunnel junctions(MTJs),have great advantages in simulating the behavior of biological synapses and neurons.This is not only because MTJs can simulate biological behavior such as spike-timing dependence plasticity and leaky integrate-fire,but also because MTJs have intrinsic stochastic and oscillatory properties.These characteristics improve MTJs’bio-fidelity and reduce their power consumption.MTJs also possess advantages such as ultrafast dynamics and non-volatile properties,making them widely utilized in the field of neuromorphic computing in recent years.We conducted a comprehensive review of the development history and underlying principles of TMR,including a detailed introduction to the material and magnetic properties of MTJs and their temperature dependence.We also explored various writing methods of MTJs and their potential applications.Furthermore,we provided a thorough analysis of the characteristics and potential applications of different types of MTJs for neuromorphic computing.TMR-based devices have demonstrated promising potential for broad application in neuromorphic computing,particularly in the development of spiking neural networks.Their ability to perform on-chip learning with ultra-low power consumption makes them an exciting prospect for future a

关键词： spintronics TMR MTJ neuromorphic computing SNN

基于MFDC-SSD网络的接触网定位线夹缺陷识别

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铁道学报 2024年第5期46卷 48-57页

作者：屈志坚张博语杨行李迪华东交通大学轨道交通基础设施性能监测与保障国家重点实验室江西南昌330013 华东交通大学电气与自动化工程学院江西南昌330013

针对接触网应用环境复杂,定位线夹体积小、安装方向特殊、不易识别,传统的目标检测算法效果较差等问题,基于多尺度特征融合密集连接网络模型,提出了一种接触网定位线夹缺陷检测新方法。首先结合DenseNet与Inception模块对SSD模型的特征... 详细信息

针对接触网应用环境复杂,定位线夹体积小、安装方向特殊、不易识别,传统的目标检测算法效果较差等问题,基于多尺度特征融合密集连接网络模型,提出了一种接触网定位线夹缺陷检测新方法。首先结合DenseNet与Inception模块对SSD模型的特征提取网络进行改进,在特定的特征层间共享上下文信息,提升特征提取能力。然后从网络深层到浅层,逐级采用FPN融合SSD检测的多尺度特征图,设计多特征融合的密集连接网络模型,最后将f GIoU作为边框损失函数,在训练中优化真实框和预测框的重合度。对采集的某段接触网定位线夹图像数据集进行检测识别。结果表明:该定位线夹缺陷检测方法可在复杂接触网背景下,对定位线夹脱落和松动进行检测,且在不同角度、亮度的图像中均具有较强的鲁棒性。

关键词：接触网缺陷识别定位线夹特征金字塔边界框损失函数

基于孪生多注意力网络的接触网侵限轻飘物跟踪

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铁道学报 2024年第2期46卷 45-55页

作者：屈志坚张博语朱琳梁家敏华东交通大学轨道交通基础设施性能监测与保障国家重点实验室江西南昌330013 华东交通大学电气与自动化工程学院江西南昌330013

针对接触网侵限轻飘物尺度变化大、遮挡干扰和铁路限界背景杂乱,易导致跟踪失败的问题,提出一种基于孪生多注意力网络的接触网轻飘物侵限跟踪新方法。引入三种注意力机制从更深层次提取轻飘物特征,通过空间注意力消除局部感受野限制,通... 详细信息

针对接触网侵限轻飘物尺度变化大、遮挡干扰和铁路限界背景杂乱,易导致跟踪失败的问题,提出一种基于孪生多注意力网络的接触网轻飘物侵限跟踪新方法。引入三种注意力机制从更深层次提取轻飘物特征,通过空间注意力消除局部感受野限制,通过通道注意力突出轻飘物类别的通道特征,将交叉注意力聚焦于目标模板与搜索图像信息,采用空间正则化滤波器抑制背景干扰,融合各部分特征实现对侵限轻飘物的跟踪。采用OTB100数据集进行精度与准度实验,以国家重点实验室试验线采集的数据为算例进行实验,通过消融实验验证了新方法的有效性。结果表明:相比于相关滤波类SRDCF算法和深度学习类SiamRPN++、DaSiamRPN算法,新方法可获得更好的鲁棒性和准确性。

关键词：接触网限界轻飘物注意力机制神经网络空间正则化