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LC-MS/MS Method Applied to the Detection and Quantification of Ursodeoxycholic Acid Related Substances in Raw Material and Pharmaceutical Formulation

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Journal of Pharmacy and Pharmacology 2018年第5期6卷 448-455页

作者： boscolo oriana Flor Sabrina Dobrecky Cecilia Martinefski Manuela Tripodi Valeria Lucangioli Silvia Silvia Lucangioli Universidad de Buenos Aires Facultad de Farmacia y Bioquimica Departamento de Tecnologia Farmacdutica Junin 956 CABAArgentina Universidad de Buenos Aires Facultad de Farmacia y Bioquimica Departamento de Quimica Analitica Junin 956 CABAArgentina Universidad de Buenos Aires Facultad de Farmacia y Bioquimica Departamento de Farmacologia Junin 956 CABA Argentina Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET) Godoy Cruz 2290 CABA Argentina 不详

Objective： To develop a highly sensitive LC-MS/MS （liquid chromatography-mass spectxometry/mass spectrometry） method applied to the detection and quantitation of UDCA （ursodeoxycholic acid） related substances such as CA （cholic acid）, DCA （deoxycholic acid）, CDCA （chenodeoxycholic acid） and LCA （lithocholic acid） in raw material and pharmaceutical formulation. Methods： The method was validated for specificity, linearity, accuracy, precision, robustness. A triple quadrupole mass detector was employed, equipped with an ESI （electrospray ionization） source operated in the negative ion mode. The chromatographic system consisted of a Symmetry C 18 column （150 mm × 4.6 mm, id; particle size 5 μm） and methanol-acetonitrile-ammonium acetate （pH 7.6; 10 mM）（40：40：20, v/v/v） as the mobile phase. The chromatographic conditions were 25 uL injection volume, flow rate of 0.4 mL/min and column temperature set at 35℃. Key tindings： The method requires a minimum sample amount and presents very low LOD （limits of detection） for CA （0.29 ng/mL）, DCA （0.59 ng/mL）, CDCA （0.13 ng/mL） and LCA （0.44 ng/mL） in comparison to LC methods coupled to different detectors like UV （ultraviolet）, fluorescence and refractive index. Conclusions： The developed and validated LC-MS/MS method for the determination of UDCA and related substances in raw material and in a suspension was advantageous since it required a minimum sample amount. In turn, it could be used as a stability indicating method.

关键词： UDCA related substances LC mass detector pharmaceutical formulation.

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Secret key exchange in ultralong lasers by radiofrequency spectrum coding

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Light(Science & Applications) 2015年第1期4卷 400-404页

作者： Alessandro Tonello Alain Barthelemy Katarzyna Krupa Vincent Kermene Agnes Desfarges-Berthelemot Badr Mohamed Shalaby Sonia boscolo Sergei K Turitsyn Juan Diego Ania-Castanon Universite de Limoges XLIMUMR CNRS 7252123 Avenue A.Thomas87060 LimogesFrance Physics Department Faculty of ScienceTanta UniversityTantaEgypt Aston Institute of Photonic Technologies Aston UniversityBirmingham B47ETUK Instituto de Optica CSICC/Serrano 121Madrid 28006Spain

We propose a new approach to the generation of an alphabet for secret key exchange relying on small variations in the cavity length of an ultralong fiber *** new concept is supported by experimental results showing how the radiofrequency spectrum of the laser can be exploited as a carrier to exchange *** test bench for our proof of principle is a 50-km-long fiber laser linking two users,Alice and Bob,where each user can randomly add an extra 1-km-long segment of *** choice of laser length is driven by two independent random binary values,which makes such length become itself a random *** security of key exchange is ensured whenever the two independent random choices lead to the same laser length and,hence,to the same free spectral range.

关键词： fiber lasers fiber optics communications optical security and encryption

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Does landscape context affect pollinationrelated functional diversity and richness of understory flowers in forest fragments of Atlantic Rainforest in southeastern Brazil?

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Ecological Processes 2020年第1期9卷 737-748页

作者： Karoline Baptista de Lima Patrícia Alves Ferreira Milton Groppo Renato Goldenberg Emerson Ricardo Pansarin Roxana Cardoso Barreto Guilherme Peres Coelho Yago Barros-Souza Danilo boscolo Biology Department Faculty of PhilosophySciences and LettersUniversity of São PauloRibeirão PretoSP 14040-901Brazil National Institute of Science and Technology in Interdisciplinary and Transdisciplinary Studies in Ecology and Evolution(IN-TREE) SalvadorBrazil Botany Department Biological Science SectorParanáFederal UniversityCuritibaPR 81531-970Brazil Botany Department Biological Science CenterPernambuco Federal UniversityRecifePE 50670-420Brazil Institute of Bioscience Rio Grande do Sul Federal UniversityPorto AlegreRS 90040-060Brazil Botany Department Federal University of São CarlosSão CarlosSP 13565-905Brazil.

Background:How landscape modifications affect functional diversity of floral characteristics pertinent to pollinators is poorly *** possess functional traits that sometimes coevolved with pollinators,crucial for the maintenance of both pollinator and plant *** evaluated how richness and functional diversity of available understory flowers respond to forest cover and landscape heterogeneity in a multiscale *** in bloom were sampled from 25 landscapes in the understory of Atlantic Forest fragments in *** were classified into functional groups regarding flower characteristics relevant to *** heterogeneity and forest cover were measured in buffers ranging from 200 to 2000 m from sampling units and their correlation with plant richness and functional diversity was assessed using generalized linear models and further model selection through Akaike’s second-order information ***:Plants’richness and functional diversity were affected negatively by forest *** former responded to forest cover at a regional scale while the latter responded at a local *** landscape heterogeneity increased richness and functional ***:Our results showed that forest cover and landscape heterogeneity are important to support biodiversity related to pollination,mostly due to the availability of diversified resources and nesting sites associated to different land-uses for pollinators and flowering plant *** findings should highlight,along with forest cover,landscape heterogeneity as an environmental management priority in rural tropical areas for mitigating the loss of plant biodiversity and enhancing ecosystem functioning.

关键词： Functional diversity Pollination Landscape heterogeneity Forest cover Understory plant community

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Physics and Considerations on millimetre-wave Free Electron Lasers with low energy electron beams

Physics and Considerations on millimetre-wave Free Electron ...

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2000 25～(th) International Conference on Infrared and Millimeter Waves Conference Digest

作者： I.boscolo M.Valentini Università via Celoria 16 20133 MilanoItaly P.J.M.van der SlotNCLRPO Box 26627500 CREnschedeThe Netherlands Università via Celoria 16 20133 MilanoItaly P.J.M.van der SlotNCLRPO Box 26627500 CREnschedeThe Netherlands

＜正＞ In the frame of the development of a millimetre wave source for industrial applications,the European Network on Microwave Applications of Industrial Free Electron Laseraims at producing a working design of a broad-band, compact and low-cost microwavesource （10-100 GHz） of sufficient power （more than 1 kW, CW or average） so as to beable to aid industrial manufacturing. For the sake of compactness, low electron beamenergies （of about 100 keV） and currents of a few A at most, are required.

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模拟失重大鼠大脑中动脉与肠系膜小动脉生物力学行为的比较

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生理学报 2009年第4期61卷 386-394页

作者：程九华 boscolo Marco 林乐健白云刚张向马进张立藩第四军医大学航空航天医学教育部重点实验室航空航天生理学教研室西安710032 克兰菲尔德大学航空工程系

为进一步阐明短期失重下血管的适应性改变及其重力性对抗措施的机理,本文对3d模拟失重组(SUS)、对抗措施组(STD,每日恢复正常站立体位1h模拟-Gx重力)及对照组(CON)大鼠大脑中动脉和肠系膜第三级小动脉的被动和主动生物力学特性进行了分... 详细信息

为进一步阐明短期失重下血管的适应性改变及其重力性对抗措施的机理,本文对3d模拟失重组(SUS)、对抗措施组(STD,每日恢复正常站立体位1h模拟-Gx重力)及对照组(CON)大鼠大脑中动脉和肠系膜第三级小动脉的被动和主动生物力学特性进行了分析。所研究的生物力学参数包括:被动血管的表观刚度(β)、被动和主动血管的周向应力(σθ)-应变(εθ)关系及其增量弹性模量(Einc,p),和平滑肌收缩活动所致的激活增量弹性模量(Einc,a)。主动血管生物力学特性分析结果显示,肌源性紧张度的调节对失重下血管的适应具有重要意义:(1)SUS组肠系膜第三级小动脉的主动σθ-εθ曲线与其被动态曲线基本一致,表明通过肌紧张度调节使σθ恢复正常的功能基本丧失;但每日1h的-Gx重力暴露则可完全防止此功能减退;(2)SUS组大脑中动脉的主动σθ-εθ曲线较其被动曲线明显左移,不同压力下εθ始终相对稳定,σθ也趋于正常;且每日短时-Gx重力不能防止这种功能亢进。另一方面,对被动血管生物力学特性的分析则提示,不同血管的间质成分可能有不同重塑(remodeling)变化,如失重暴露时间进一步延长,将可能观察到有显著意义的重要变化。总之,本文血管生物力学特性分析对阐明失重环境下血管适应机理及其重力性对抗措施具有重要意义。

关键词：微重力应力-应变关系增量弹性模量刚度肌源性紧张度大脑中动脉:肠系膜小动脉

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Fruit Waste and Sugarcane Bagasse as Potential Natural Resources of Mineral and Lipophilic Substances

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Journal of Food Science and Engineering 2015年第3期5卷 137-141页

作者： Sonia Patricia Ordonez Crispin Humberto Garcia-Cruz Mauricio boscolo Jesus Eliecer Larrahondo Department of Engineering and Technology of Food Sao Paulo State University (UNESP) 1B1LCE Campus of S6o Jose Do RioPreto Sao Jose Do Rio Preto CEP 15000 Brazill Department of Chemistry and Environment Science Sao Paulo State University (UNESP) IBILCE Campus of Sao Jose Do RioPreto CEP 15000 Brazil Department of Chemistry Santiago Call University. Santiago de Call 760035 Colombia

The objectives of this study were to explore alternatives for using fruit waste and sugarcane bagasse as important sources for new products and potential applications in the food industry. Fast foods are part of modern life, as well as sources for producing biofuels based on biomass. The mineral content and compounds of nutritional interest, such as lipophilic substances, were determined in fruit waste （orange peels, passion fruit, bananas, grapes） and sugarcane bagasse. Significant differences were found in the samples tested, where minerals, such as potassium, nitrogen, zinc and iron, were found in fruit residues （bananas, oranges, passion fruit） and sugarcane bagasse. Banana residues were the most important source of minerals, followed by orange peels. Gas chromatography mass spectrography （GC-MS） analyses of the lipophilic fractions obtained from the plant residues revealed the presence of mostly saturated （palmitic, stearic） and unsaturated （oleic and linoleic） fatty acids, as well as other nutritionally valuable compounds, such as antioxidants （flavones in orange residues）. The residues studied here can be used for future research to optimize pretreatment and hydrolysis of biomass for bioethanol production.

关键词： Biomass fruit waste minerals lipophilic substances sugarcane bagasse

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模拟失重大鼠肠系膜小动脉的力学特性与重塑变化及重力性对抗措施的防护效果(英文)

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生理学报 2012年第2期64卷 107-120页

作者：高放程九华白云刚 Marco boscolo 黄晓峰张向张立藩第四军医大学航空航天生理学教研室及教育部航空航天医学重点实验室西安710032 第四军医大学中心实验室西安710032 克兰菲尔德大学航空工程系克兰菲尔德MK430AL英国

为进一步阐明中期模拟失重环境下,肠系膜阻力性小血管的适应性改变及重力性对抗措施的防护效果,本文对28d模拟失重组(SUS)、对抗措施组(S+D:每日使动物恢复正常站立体位1h,承受-Gx方向的重力作用;其余23h仍处于模拟失重状态),以及对照组... 详细信息

为进一步阐明中期模拟失重环境下,肠系膜阻力性小血管的适应性改变及重力性对抗措施的防护效果,本文对28d模拟失重组(SUS)、对抗措施组(S+D:每日使动物恢复正常站立体位1h,承受-Gx方向的重力作用;其余23h仍处于模拟失重状态),以及对照组(CON)大鼠肠系膜第三级小动脉的生物力学特性与血管壁重塑变化进行了比较。所计算的生物力学参数包括:血管在被动状态下的表观刚度(β);血管在主动和被动状态下的周向应力(σθ)-应变(εθ)关系及其增量弹性模量(Einc,p)。血管壁胶原和弹性蛋白的数量则以其在高倍透射电镜下截面积所占的百分比定量。结果显示,SUS组的主动σθ-εθ曲线非常靠近血管的被动曲线,表明其肌源性紧张度调节功能已基本丧失;但S+D的主动曲线则非常靠近CON组,二者都远离被动曲线,明显左移,并呈抛物线状。S+D组的主动曲线反映跨壁压升高时,血管刚度主动增大使应变降低的过程;但重力性对抗措施似仍不能使其与CON组的血管完全相当。三组血管的被动生物力学特性未见有显著性差别:三组的β均值介于6.3～6.8;三组的被动σθ-εθ曲线及Einc,p随压力的变化皆呈指数型增长;上述被动态参数在三组间皆无显著性的差别。然而,三组血管胶原蛋白截面积的百分比却有显著性差别。电镜图像显示,CON与S+D组大鼠中膜平滑肌细胞间隙胶原的面积显然远大于SUS组。形态测量表明:与CON相比,S+D与SUS组管壁中膜胶原蛋白的截面积分别减少了27%与46%(P<0.01);而S+D与SUS组间的差别也具有显著性意义(P<0.05)。以上结果表明,除胶原与弹性蛋白的数量与比例因素外,血管的被动生物力学特性还取决于其它一些因素。本文从生物力学角度进一步阐明了航天飞行后立位耐力不良以及重力性对抗措施的机理。

关键词：肌源性紧张度和反应性血管重塑飞行后心血管功能失调间断性人工重力

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Performance and Muscular Development of Nile Tilapia Larvae (Oreochromis niloticus) Fed Increasing Concentrations of Phenylalanine

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Agricultural Sciences 2016年第12期7卷 900-910页

作者： Diogo Yamashiro Dacley Hertes Neu Evandro Bilha Moro Evandro Bilha Moro Aldi Feiden Altevir Signor Wilson Rogério boscolo Fábio Bittencourt Cooperativa Agroindustrial Consolata Nova Aurora Brazil Faculdade de Ciências Agrarias-FCA Universidade Federal da Grande Dourados Dourados Brazil Centro de Engenharias e Ciências Exatas Universidade Estadual do Oeste do Paraná Toledo Brazil

Even though tilapia is of great economic interest, data on the nutritional requirements of amino acids during commercial breeding stages are scarce. So, the aim of this study was to analyse the performance and muscular growth of Nile tilapia larvae (Oreochromis niloticus) fed diets containing increasing concentrations of phenylalanine. The experiment took place at the Laboratory of Aquaculture of the Grupo de Estudos de Manejo na Aquicultura (GEMAq) of the Universidade Estadual do Oeste do Paraná (Unioeste), Toledo, PR, Brazil, for 30 days. A total of 450 larvae, with mean initial length of 1.5 ± 0.14 cm and mean initial weight of 0.04 ± 0.004 g, were randomly distributed into 6 treatment groups with 5 repetitions (30 tanks). Each experimental unit consisted of a 30 L tank containing 15 larvae. Six diets were formulated with increasing concentrations of phenylalanine (1.09, 1.24, 1.39, 1.54, 1.69 and 1.84%) and offered four times a day until apparent satiation. By the end of the experimental period, the following productivity indexes were analysed: length, weight, survival, weight gain, uniformity, feed conversion, specific growth rate and protein efficiency ratio. Three fish from each tank were collected for muscular growth analysis and the frequency of small ( 0.05) was observed in the productive performance parameters between the treatment groups. Thus, it can be concluded that the levels of phenylalanine contained in the experimental diets did not influence the performance of Nile tilapia larvae. However, there was more hyperplasia when we put 1.39% of phenylalanine in the diet.

关键词： Amino Acids Amino Acid Requirements Larval Fish Nutrition

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普萘洛尔靶向抑制血管瘤周细胞

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中国口腔颌面外科杂志 2014年第3期 232-232页

作者： Lee D boscolo E Durham JT

普萘洛尔是非选择性β肾上腺素受体(AR)阻滞剂,偶然发现其治疗严重婴幼儿血管瘤(IH)效果显著。服药后短时间内即可发现肿瘤颜色变浅,几周或数月之后肿瘤消退。该文通过对增殖期和消退期血管瘤周细胞进行检测,分析周细胞在普萘洛尔诱导... 详细信息

普萘洛尔是非选择性β肾上腺素受体(AR)阻滞剂,偶然发现其治疗严重婴幼儿血管瘤(IH)效果显著。服药后短时间内即可发现肿瘤颜色变浅,几周或数月之后肿瘤消退。该文通过对增殖期和消退期血管瘤周细胞进行检测,分析周细胞在普萘洛尔诱导血管瘤消退中的作用。与膀胱平滑肌细胞相比,血管瘤周细胞高表达β2ARmRNA。此外,在15例IH标本中,β2ARmRNA的表达水平较β1AR、β3AR及α1bAR均升高。血管瘤周细胞的抑制性实验在硅

关键词：周细胞普萘洛尔血管瘤血管肿瘤

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整合蛋白α6在血管瘤干细胞黏附及血管新生中的作用

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中国口腔颌面外科杂志 2013年第6期 490-490页

作者： Smadja DM Guerin CL boscolo E

婴幼儿血管瘤(IH)是婴幼儿最常见的肿瘤。血管瘤干细胞(HemSC)是一种来源于胚胎间充质的CD133+细胞。HemSC注入裸鼠体内后,可以分化为内皮细胞和周细胞/平滑肌细胞,形成血管网。整合蛋白α6亚族已被证明与恶性胶质瘤干细胞的致瘤作用,... 详细信息

婴幼儿血管瘤(IH)是婴幼儿最常见的肿瘤。血管瘤干细胞(HemSC)是一种来源于胚胎间充质的CD133+细胞。HemSC注入裸鼠体内后,可以分化为内皮细胞和周细胞/平滑肌细胞,形成血管网。整合蛋白α6亚族已被证明与恶性胶质瘤干细胞的致瘤作用,血管生成及内皮、间充质祖细胞有关。该文旨在对整合蛋白α6在HemSC中的作用进行研究。记

关键词：血管瘤细胞血管肿瘤整合蛋白层黏连蛋白

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