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Roles of paroxetine and corticosterone on adult mammalian ciliary body cell proliferation

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Chinese Medical Journal 2010年第10期123卷 1305-1310页

作者： WANG Hua LAU Benson WM YAU Suk-yu LI Suk-yee LEUNG Nelson WANG Ning-li tang siu-wa LEE Tatia MC SO Kwok-fai Department of Anatomy Li Ka Shing Faculty of Medicine the University of Hong Kong Hong Kong Special Administrative Region China the State Key Laboratory of Brain and Cognitive Sciences the University of Hong Kong Hong Kong Special Administrative Region China Neuropsychology Laboratory the University of Hong Kong Hong Kong Special Administrative Region China Beijing Tongren Eye Center Beijing Tongren Hospital Capital Medical University Beijing Ophthalmology & Visual Sciences Key Laboratory Beijing 100730 China Joint Laboratory for Brain Function and Health (BFAH) Jinan University and the University of Hong Kong

Background The neurogenesis in retina of adult mammals is generally abolished, and this renders the retina lack of regenerative capacity. Despite this, there is a small population of nestin-positive cells in the ciliary epithelium which retains neurogenic potential. The present study aimed at investigating the effect of two drugs, corticosterone and paroxetine, on the cell proliferation of the ciliary body. Methods Adult Sprague-Dawley rats were given vehicle, corticosterone, paroxetine, or both corticosterone and paroxetine treatment for 14 days. Cell proliferation in the ciliary body was quantified using 5-bromo-2-deoxyuridine （BrdU） immunohistochemistry. Co-labelling of BrdU and stem cell marker was used to phenotype the BrdU immunoreactive cells. Results Corticosterone treatment suppressed while paroxetine treatment increased the cell proliferation of the ciliary body. Co-labelling with cell markers revealed that the BrdU positive cells also showed nestin expression but not glial fJbrillary acidic protein （GFAP）. Conclusions The results illustrate that proliferation of retinal progenitor cells situated in ciliary body are subjected to regulation by selective serotonin reuptake Jnhibitors （SSRI） and corticosteroJd, which is similar to our previous findings in neurogenic regions in central nervous system （CNS）. Paroxetine treatment could reverse the suppressive effect of corticosterone on ciliary body cell proliferation. This provides information for future investigation of retinal stem cell biology and potential treatment of retinal degenerative diseases.

关键词： paroxetine 5-bromo-2-deoxyuridine corticosterone ciliary body cell proliferation neurogenesis

Low dose of corticosterone treatment with exercise increases hippocampal cell proliferation, and improves cognition

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Neural Regeneration Research 2011年第34期6卷 2645-2655页

作者： Suk-Yu Yau Jada Chia-Di Lee Benson Wui-Man Lau Tatia M.C. Lee Yick-Pang Ching siu-Wa tang Kwok-fai So Department of Anatomy LKS Faculty of Medicine the University of Hong Kong State Key Laboratory of Brain and Cognitive Science LKS Faculty of Medicine the University of Hong Kong Research Centre of Heart Brain Hormone and Health Aging LKS Faculty of Medicine the University of Hong Kong Laboratory of Neuropsychology the University of Hong Kong Laboratory of Cognitive Affective Neuroscience the University of Hong Kong Joint Laboratory for Brain Function and Health (BFAH) Jinan University and the University of Hong Kong

Intermediate level of stress is beneficial for brain functions, whereas extreme low level or high level of stress is deleterious. We have previously shown that chronic exposure to high doses of corticosterone （CORT） suppressed hippocampal plasticity and physical exercise in terms of running counteracted the detrimental effects of CORT treatment. We aimed to study whether a mild stress, that mimicked by a treatment with low CORT dose, improved hippocampal plasticity in terms of hippocampal cell proliferation and dendritic remodeling, and to examine whether running with CORT treatment showed an additive effect on improving hippocampal plasticity. The rats were treated with 20 mg/kg CORT for 14 days with or without running, followed by Morris water maze test or forced swim test. The hippocampal proliferating cells was labeled by intraperitoneal injection of 5-bromo-2＇-deoxyuridine. The dendritic morphology was analyzed using Golgi staining method. Treatment with 20 mg/kg CORT alone yielded a higher number of hippocampal cell proliferation and significantly increased dendritic branching compared to vehicle-treated non-runners, but had no behavioral effects. In contrast, CORT treatment with running showed an additive increase in hippocampal cell proliferation and dendritic remodeling that was associated with improved spatial learning and decreased depression-like behavior; however, there was no additive improvement in behavior compared to vehicle-treated runners. These findings suggest that mild stress does not always cause detrimental effect on the brain, and combining mild stress with running could promote hippocampal plasticity via inducing cell proliferation and dendritic remodeling.

关键词： hippocampal cell proliferation physical exercise spatial learning stress structural plasticity neural plasticity

Molecular basis of von Hippel-Lindau syndrome in Chinese patients

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Chinese Medical Journal 2011年第2期000卷 237-241页

作者： siu Wai-kwan MA Ronald Ching-wan LAM Ching-wan MAK Chloe Miu YUEN Yuet-ping LO fai-man Ivan CHAN Kin-wan LAM siu-fung LING siu-cheung TONG Sui-fan SO Wing-yee CHOW Chun-chung tang Mary Hoi-yin TAM wing-hung CHAN Albert Yan-wo Department of Pathology Princess Margaret Hospital Hong Kong China Department of Medicine & Therapeutics The Chinese University of Hong Kong Prince of Wales Hospital Hong Kong China Department of Pathology Li Ka Shing Faculty of Medicine The University of Hong Kong Queen Mary Hospital Hong Kong China Clinical Genetic Service Department of Health Hong Kong China Department of Medicine and Geriatrics Princess Margaret Hospital Hong Kong China Department of Paediatrics and Adolescent Medicine Princess Margaret Hospital Hong Kong China Department of Obstetrics & Gynaecology Tsan Yuk Hospital Hong Kong China Department of Obstetrics & Gynaecology The Chinese University of Hong Kong Prince of Wales Hospital Hong Kong China

Background Von Hippel-Lindau （VHL） syndrome is an autosomal dominant familial cancer syndrome predisposing the affected individuals to multiple tumours in various *** genetic basis of VHL in Southern Chinese is largely *** this study,we characterized the mutation spectrum of VHL in nine unrelated Southern Chinese *** Nine probands with clinical features of VHL,two symptomatic and eight asymptomatic family members were included in this *** diagnosis was performed twice for one *** probands had only isolated bilateral *** VHL gene was screened for mutations by polymerase chain reaction,direct sequencing and multiplex ligation-dependent probe amplification （MLPA）.Results The nine probands and the two symptomatic family members carried heterozygous germline *** different VHL mutations were identified in the nine *** splicing mutation,NM_000551.2：c.463＋1G〉T,was *** other seven VHL mutations,c.233A〉G [***78Ser],c.239G〉T [***80lle],c.319C〉G [***107Gly],c.481C〉T [***161X],c.482G〉A [***161GIn],c.499C〉T [***167Trp] and an exon 2 deletion,had been previously *** asymptomatic family members were positive for the mutation and the other five tested *** prenatal diagnosis,the fetuses were positive for the *** Genetic analysis could accurately confirm VHL syndrome in patients with isolated tumours such as sporadic phaeochromocytoma or epididymal papillary *** detection in asymptomatic family members allows regular tumour surveillance and early intervention to improve their ***-based diagnosis can have an important impact on clinical management for VHL families.

关键词： von Hippel-Lindau syndrome VHL gene Chinese VHL mutations

Corticosterone suppresses dendritic maturation of immature neurons in hippocampus,which is counteracted by antidepressant treatment

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Corticosterone suppresses dendritic maturation of immature n...

Modulation of the suppressive effect of corticosterone on adult rat hippocampal cell proliferation by paroxetine

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Benson WM LAU Suk-Yu YAU Tatia M.C.LEE Yick-Pang CHING siu-Wa tang Kwok-fai SO Department of Anatomy Li Ka Shing Faculty of MedicineThe University of Hong Kong The State Key Laboratory of Brain and Cognitive Sciences The University of Hong Kong Research Centre of Heart BrainHormone and Healthy AgingFaculty of MedicineThe University of Hong Kong The State Key Laboratory of Brain and Cognitive Sciences The University of Hong KongPokfulam Laboratory of Neuropsychology The University of Hong Kong Department of Anatomy Li Ka Shing Faculty of MedicineThe University of Hong KongPokfulam Joint Laboratory for Brain function and Health(BFAH) Jinan University and The University of Hong Kong

＜正＞Objective Chronic corticosterone treatment was shown to suppress neurogenesis in hippocampus,while antidepressant treatment increases new hippocampal neuron *** previously showed that the suppressed neurogenesis in hippocampus could be attenuated by simultaneous selective serotonin reuptake inhibitor（SSRI） treatment. However,it is unknown whether the dendritic maturation of immature neurons is affected by these two classes of drugs. Method Sprague-Dawley rats were divided into four groups:（1）.corticosterone treatment,（2）.Paroxetine（a SSRI） treatment,（3）.co-treatment of corticosterone and paroxetine and（4）.control group with vehicle *** two weeks’ treatment,animals were killed and brain sections were immunostained with anti-doublecortin（DCX,an immature neuron marker） *** morphologies of immature neurons were analyzed by Sholl *** tissue was collected for brain-derived neurotrophic factor ELISA assay and DCX *** Compared to control group, immature neurons of corticosterone group showed a decreased dendritic *** contrast,paroxetine treatment increased the dendritic ***-treatment group showed dendritic complexity which is similar to control group. Hippocampal BDNF level is significantly decreased in corticosterone treatment group while co-treatment with paroxetine reversed the *** treatment also increased the expression level of DCX in *** Apart from neurogenesis,the present study shows that corticosterone and SSRI also affect dendritic growth of immature *** effect is associated with alternated hippocampal BDNF and DCX expression *** finding provides a new perspective on how stress hormone and antidepressant affect neuroplasticity,which may underlie the pathology of depressive and other psychiatric disorders.

关键词： corticosterone stress SSRI selective serotonin reuptake inhibitors neurogenesis doublecortin DCX hippocampus

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Neuroscience Bulletin 2007年第3期23卷 131-136页

作者：邱光 Daiga M.HELMESTE Asanka N.SAMARANAYAKE 刘汇文李湄珍邓兆华苏国辉香港大学医学院解剖学系加州大学尔湾分校精神医学系香港大学医学院精神医学系香港大学社会科学院心理学系脑神经心理实验室

Objective The literature has shown that cognitive and emotional changes may occur after chronic treatment with glucocorticoids. This might be caused by the suppressive effect of glucocorticoids on hippocampal neurogenesis and cell proliferation. Paroxetine, a selective serotonin reuptake transporter, is a commonly used antidepressant for alleviation of signs and symptoms of clinical depression. It was discovered to promote hippocampal neurogenesis in the past few years and we wanted to investigate its interaction with glucocorticoid in this study. Methods Adult rats were given vehicle, corticosterone, paroxetine, or both corticosterone and paroxetine for 14 d. Cell proliferation in the dentate gyrus was quantified using 5-bromo-2-deoxyuridine （BrdU） immunohistochemistry. Results The corticosterone treatment suppressed while paroxetine treatment increased hippocampal cell proliferation. More importantly, paroxetine treatment could reverse the suppressive effect of corticosterone on hippocampal cell proliferation. Conclusion This may have clinic application in preventing hippocampal damage after glucocorticoid treatment.

关键词： paroxetine bromodeoxyuridine corticosterone hippocampus cell proliferation neurogenesis