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GreenPhos,a universal method for in-depth measurement of plant phosphoproteomes with high quantitative reproducibility

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Molecular Plant 2024年第1期17卷 199-213页

作者： Xiaoxiao Duan Yuanya Zhang Xiahe Huang Xiao Ma Hui Gao Yan Wang Zhen Xiao Chengcheng Huang Zhongshu Wang bolong li Wenqiang Yang Yingchun Wang State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental BiologyChinese Academy of SciencesBejing 100101China University of Chinese Academy of Sciences Bejing 100049China Key Laboratory of Photobiology Instute of BotanyChinese Academy of SciencesBejing 100093China

Protein phosphorylation regulates a variety of important cellular and physiological processes in plants.In-depth profiling of plant phosphoproteomes has been more technically challenging than that of animal phosphoproteomes.This is largely due to the need to improve protein extraction efficiency from plant cells,which have a dense cell wall,and to minimize sample loss resulting from the stringent sample clean-up steps required for the removal of a large amount of biomolecules interfering with phosphopeptide purification and mass spectrometry analysis.To this end,we developed a method with a streamlined workflow for highly efficient purification of phosphopeptides from tissues of various green organisms including Arabidopsis,rice,tomato,and Chlamydomonas reinhardtii,enabling in-depth identification with high quantitative reproducibility of about 11000 phosphosites,the greatest depth achieved so far with single liquid chromatography-mass spectrometry(LC-MS)runs operated in a data-dependent acquisition(DDA)mode.The mainstay features of the method are the minimal sample loss achieved through elimination of sample clean-up before protease digestion and of desalting before phosphopeptide enrichment and hence the dramatic increases of time-and cost-effectiveness.The method,named GreenPhos,combined with single-shot LC-MS,enabled in-depth quantitative identification of Arabidopsis phosphoproteins,including differentially phosphorylated spliceosomal proteins,at multiple time points during salt stress and a number of kinase substrate motifs.GreenPhos is expected to serve as a universal method for purification of plant phosphopeptides,which,if samples are further fractionated and analyzed by multiple LC-MS runs,could enable measurement of plant phosphoproteomes with an unprecedented depth using a given mass spectrometry technology.

关键词： plant phosphoproteomics phosphopeptides LC–MS Arabidopsis salt stress

MoS2 nanoflowers and PEDOT:PSS nanocomposite enabling wearable dual-mode pressure sensors

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Science China Technological Sciences 2024年第6期67卷 1737-1747页

作者： WANG FengMing YANG WeiJia MA Ke SHEN GengZhe SU DaoJian li BaiJun WANG ShuangPeng QIN bolong ZHANG Chi XIN Yue CAO XiaoBing HE Xin School of Applied Physics and Materials Wuyi University Biomaterials R&D Center Zhuhai Institute of Advanced TechnologyChinese Academy of Sciences Joint Key Laboratory of the Ministry of Education Institute of Applied Physics and Materials EngineeringUniversity of Macau

A versatile sensing platform employing inorganic MoS2nanoflowers and organic poly（3,4-ethylene dioxythiophene）: poly（styrene sulfonate）(PEDOT:PSS) has been investigated to develop the resistive and capacitive force-sensitive devices. The microstructure of the sensing layer heightens the sensitivity and response time of the dual-mode pressure sensors by augmenting electron pathways and inner stress in response to mechanical stimuli. Consequently, the capacitive and resistive sensors exhibit sensitivities of 0.37 and 0.12 kPa-1, respectively, while demonstrating a remarkable response time of approximately 100 ms.Furthermore, it is noteworthy that the PEDOT:PSS layer exhibits excellent adhesion to polydimethylsiloxane（PDMS） substrates, which contributes to the development of highly robust force-sensitive sensors capable of enduring more than 10000loading/unloading cycles. The combination of MoS2/PEDOT:PSS layers in these dual-mode sensors has shown promising results in detecting human joint movements and subtle physiological signals. Notably, the sensors have achieved a remarkable precision rate of 98% in identifying target objects. These outcomes underscore the significant potential of these sensors for integration into applications such as electronic skin and human-machine interaction.

关键词： MoS2/PEDOT:PSS nanocomposite force-sensitive sensor multi-scaled microstructure physiological signals monitoring object grasping recognition

结泥条件下TBM滚刀破岩能量演化机制及掘进参数优化研究

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矿业研究与开发 2024年第5期44卷 201-210页

作者：张鹏赵腾跃李元龙刘勃龙北京市政路桥股份有限公司北京100045 绍兴文理学院土木工程学院浙江绍兴市312000

岩石隧道掘进机(Tunnel Boring Machine,TBM)在泥岩地层掘进过程中,渣土易形成泥浆黏附在滚刀和刀盘表面,出现刀盘结“泥饼”现象,严重降低掘进效率。为了揭示结泥对TBM滚刀破岩效率的影响,防止滚刀结泥,使用离散元程序PFC3D模拟TBM滚... 详细信息

岩石隧道掘进机(Tunnel Boring Machine,TBM)在泥岩地层掘进过程中,渣土易形成泥浆黏附在滚刀和刀盘表面,出现刀盘结“泥饼”现象,严重降低掘进效率。为了揭示结泥对TBM滚刀破岩效率的影响,防止滚刀结泥,使用离散元程序PFC3D模拟TBM滚刀回转切割泥岩过程,计算了系统总能量E、弹性应变能Ee、摩擦能E_μ、阻尼能E_β、动能Ek和黏结能Ea,从能量演化角度研究结泥条件下TBM滚刀破岩机制。随后分析了不同刀盘推力、滚刀刀刃角和刀刃宽度下的TBM滚刀破岩过程中的能量特征,根据“累积热量和累积黏结能最低,且滚刀表面黏附的泥化物质量最小”的原则,对上述掘进参数进行优化。研究结果表明,PFC3D中的黏聚滚动阻力线性模型可实现滚刀结泥过程;在结泥情况下,泥化物的形成将显著提高机械做功,消耗大量能量,降低滚刀破岩效率;提高刀盘推力可以促进泥化物的形成,导致刀盘升温,诱发泥化物黏附在滚刀表面。选用刀刃角为40°的V形滚刀,设最优刀刃宽度为13 mm,可减小滚刀结泥情况。研究结果可为相关工程中预防刀盘结“泥饼”提供理论参考。

关键词：岩石隧道掘进机结泥能量破岩机制掘进参数

Advanced MOF-based electrode materials for supercapacitors and electrocatalytic oxygen reduction

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Nano Research 2023年第1期16卷 1338-1361页

作者： bolong Yang Bingjie li Zhonghua Xiang State Key Laboratory of Organic-Inorganic Composites Beijing University of Chemical TechnologyBeijing 100029China The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052China

Metal-organic frameworks(MOFs)have attracted a lot of attention due to their diverse structures,favorable porous properties,and tunable chemical compositions in the multiple fields.Notably,MOF-based materials(including pristine MOFs,MOF composites,and their derivatives)play the vital role in electrochemical energy storage and conversion systems,due to their ability for regulating chemical composition at the molecular level and their highly porous frameworks for facilitating the mass and charge transfer.Supercapacitors and fuel cells are used as one of energy storage and conversion systems respectively,and it is unstoppable to design and synthesize high-efficiency electrode materials for them.This review starts with the strategies for designing targeted MOF-based materials in electrochemical energy storage and conversion applications followed by the state-ofthe-art MOF-based materials discussed as to their potential applications in supercapacitors and electrocatalytic oxygen reduction reaction(ORR).Finally,the challenges and perspectives of MOF-based materials applied for supercapacitors and electrocatalytic ORR are discussed.

关键词： metal-organic frameworks(MOFs) MOF composites MOF derivatives supercapacitors electrocatalytic oxygen reduction reaction(ORR)

Multifunctional mesoporous silica nanoparticles for biomedical applications

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Signal Transduction and Targeted Therapy 2023年第12期8卷 5807-5834页

作者： bolong Xu Shanshan li Rui Shi Huiyu liu Beijing Advanced Innovation Center for Soft Matter Science and Engineering State Key Laboratory of Organic-Inorganic CompositesBionanomaterials&Translational Engineering LaboratoryBeijing Key Laboratory of BioprocessBeijing Laboratory of Biomedical MaterialsBeijing University of Chemical Technology100029 BeijingChina National Center for Orthopaedics Beijing Research Institute of Traumatology and OrthopaedicsBeijing Jishuitan Hospital100035 BeijingChina

Mesoporous silica nanoparticles(MSNs)are recognized as a prime example of nanotechnology applied in the biomedical field,due to their easily tunable structure and composition,diverse surface functionalization properties,and excellent biocompatibility.Over the past two decades,researchers have developed a wide variety of MSNs-based nanoplatforms through careful design and controlled preparation techniques,demonstrating their adaptability to various biomedical application scenarios.With the continuous breakthroughs of MSNs in the fields of biosensing,disease diagnosis and treatment,tissue engineering,etc.,MSNs are gradually moving from basic research to clinical trials.In this review,we provide a detailed summary of MSNs in the biomedical field,beginning with a comprehensive overview of their development history.We then discuss the types of MSNs-based nanostructured architectures,as well as the classification of MSNs-based nanocomposites according to the elements existed in various inorganic functional components.Subsequently,we summarize the primary purposes of surface-functionalized modifications of MSNs.In the following,we discuss the biomedical applications of MSNs,and highlight the MSNs-based targeted therapeutic modalities currently developed.Given the importance of clinical translation,we also summarize the progress of MSNs in clinical trials.Finally,we take a perspective on the future direction and remaining challenges of MSNs in the biomedical field.

关键词： nanoparticles breakthrough inorganic

分步负载金属法制备铁钴双金属位点高效氧还原电催化剂

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化工学报 2024年第4期75卷 1578-1593页

作者：贾旭东杨博龙程前李雪丽向中华北京化工大学有机无机复合材料国家重点实验室北京100029

过渡金属和氮掺杂的碳(M-N-C)由于优异的电催化活性以及较低的生产成本,成为铂基催化剂的替代。然而,目前的M-N-C催化剂通常涉及金属盐、含氮物质和碳载体的结合,在经过热处理和酸洗过程后得到的催化剂在活性位点密度和传质能力上性能... 详细信息

过渡金属和氮掺杂的碳(M-N-C)由于优异的电催化活性以及较低的生产成本,成为铂基催化剂的替代。然而,目前的M-N-C催化剂通常涉及金属盐、含氮物质和碳载体的结合,在经过热处理和酸洗过程后得到的催化剂在活性位点密度和传质能力上性能不足。采用分步负载金属法制备了Fe、Co双金属掺杂的M-N-C催化剂。利用Zn2+和Co2+的竞争效应,成功合成了小尺寸且均匀的Zn-Co-ZIFs双金属沸石咪唑骨架。随后,在不形成金属团簇的前提下将最大量的Fe原子嵌入C-Zn-Co-ZIFs-H+前体结构中,使其在热解后能产生大量的FeCo—Nx活性位点。这种改进显著增加了FeCo-N-C-2催化剂中Fe活性位点的含量(1.9%,质量分数),并深度优化了其微孔和介孔结构(860 m^(2)·g^(-1))。该催化剂在0.1 mol·L^(-1) HClO_(4)和0.1 mol·L^(-1) KOH溶液中,氧还原反应(ORR)活性展现出了0.806 V和0.921 V的半波电位,并分别在50000、45000 s恒定电位测试后保持了91.21%和95.32%的活性。将其组装于质子交换膜燃料电池(PEMFCs)和碱性锌-空气液流电池(ZAFBs)中,峰值功率密度分别达到了746、164 mW·cm^(-2),显示出优越的性能。

关键词：催化剂分步负载金属法铁钴双金属位点电化学氧还原反应燃料电池锌-空气液流电池

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Comparative study of the adsorption performance of NH_(2)-functionalized metal organic frameworks with activated carbon composites for the treatment of phenolic wastewaters

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Chinese Journal of Chemical Engineering 2023年第11期63卷 130-139页

作者： bolong Jiang Shunjie Shi Yanyan Cui Jiayou li Nan Jiang Yanguang Chen Innovation Institute for Sustainable Maritime Architecture Research and Technology Qingdao University of TechnologyQingdao 266000China College of Chemistry and Chemical Engineering Northeast Petroleum UniversityDaqing 163318China Institute of Environmental and Municipal Engineering Qingdao University of TechnologyQingdao 266000China

To better understand the role of the-NH_(2)group in adsorption process of phenolic wastewaters,NH_(2)-functionalized MIL-53(Al)composites with activated carbon(NH_(2)-M(Al)@(B)AC)were prepared.The results showed that the-NH_(2)group could increase the mesopore volume for composites,which promotes mass transfer and full utilization of active sites,because hierarchical mesopore structure makes the adsorbent easier to enter the internal adsorption sites.Furthermore,the introduction of the-NH_(2)group can improve the adsorption capacity,decrease the activation energy,and enhance the interaction between the adsorbent and p-nitrophenol,demonstrating that the-NH_(2)group plays a crucial role in the adsorption of p-nitrophenol.The density functional theory calculation results show that the H-bond interaction between the-NH_(2)group in the adsorbent and the-NO_(2)in the p-nitrophenol(adsorption energy of -35.5 kJ·mol^(-1)),and base-acid interaction between the primary-NH_(2)group in the adsorbent and the acidic-OH group in the p-nitrophenol(adsorption energy of -27.3 kJ·mol^(-1))are predominant mechanisms for adsorption in terms of the NH_(2)-functionalized adsorbent.Both NH_(2)-functionalized M(Al)@AC and M(Al)@BAC composites exhibited higher p-nitrophenol adsorption capacity than corresponding nonfunctionalized composites.Among the composites,the NH_(2)-M(Al)@BAC had the highest p-nitrophenol adsorption capacity of 474 mg·g^(-1).

关键词： NH_(2)-functionalized adsorbent Phenolic wastewaters Adsorption Mechanism Density functional theory

吉林莫莫格国家级自然保护区河流湿地的鱼类栖息地修复效果评价

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湿地科学 2024年第1期22卷 1-15页

作者：杨富亿文波龙李晓宇刘宏远邹畅林中国科学院湿地生态与环境重点实验室中国科学院东北地理与农业生态研究所吉林长春130102 吉林莫莫格国家级自然保护区管理局吉林白城137300

鱼类是水禽栖息地及其迁徙停歇地中的重要食物基础。吉林莫莫格国家级自然保护区是白鹤(Grus leucogeranus)和东方白鹳(Ciconia boyciana)等珍稀濒危鸟类迁徙的重要停歇地。针对该保护区中的鱼类资源增殖能力下降、水禽栖息地及其迁徙... 详细信息

鱼类是水禽栖息地及其迁徙停歇地中的重要食物基础。吉林莫莫格国家级自然保护区是白鹤(Grus leucogeranus)和东方白鹳(Ciconia boyciana)等珍稀濒危鸟类迁徙的重要停歇地。针对该保护区中的鱼类资源增殖能力下降、水禽栖息地及其迁徙停歇地的适宜性水平降低的现状,2016年至2017年期间,采用湿地水文和基底修复的方法,对嫩江近岸浅水区和洪泛区中鱼类的自然栖息地进行了修复;鱼类栖息地修复前、后,分别于2015年9月和2017年11月至2021年9月期间,在鱼类栖息地,开展了鱼类样本采集和调查,研究了鱼类栖息地修复前、后鱼类群落的组成和结构特征,评价了鱼类栖息地的修复效果。研究结果表明,与鱼类自然栖息地相比,修复栖息地的鱼类物种数量明显增多,而经济鱼类的相对种群生物量和相对种群数量却分别减小了16.02%和39.98%,小型非经济鱼类的相对种群生物量和相对种群数量比自然栖息地分别增大了1.43倍和63.54%,鱼类现存资源量、平均年鱼类现存资源量和平均年鱼类分布密度大幅增加;修复栖息地鱼类群落的Simpson多样性指数和Pielou均匀度指数比自然栖息地分别减小了31.98%和34.42%。所采用的湿地水文和基底修复方法,提高了修复栖息地对鱼类的适宜性,促进了鱼类资源自然增殖和种群自我发展,实现了嫩江近岸浅水区和洪泛区作为水禽栖息地及其迁徙停歇地功能的可持续性,弥补了自然栖息地适宜性差、鱼类自然增殖能力低和种群发展不可持续的缺陷。该修复方法可以为松嫩平原湿地型自然保护区鱼类栖息地修复提供借鉴。

关键词：鱼类栖息地修复河流湿地吉林莫莫格国家级自然保护区

A highly ionic conductive succinonitrile-based composite solid electrolyte for lithium metal batteries

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Nano Research 2022年第6期15卷 5153-5160页

作者： Genrui Qiu Yapeng Shi bolong Huang CAS Center for Excellence in Nanoscience Beijing Institute of Nanoenergy and NanosystemsChinese Academy of SciencesBeijing 100083China School of Nanoscience and Technology University of Chinese Academy of SciencesBeijing 100049China Department of Applied Biology and Chemical Technology The Hong Kong Polytechnic UniversityHung HomKowloonHong Kong 999077China

Solid-state li metal batteries with solid electrolytes have built a potential way to solve the safety and low energy density problems of current commercial li-ion batteries with liquid electrolyte.As a key component of solid-state li metal batteries,solid electrolytes require high ionic conductivities and good mechanical properties.We have designed a composite solid electrolyte(CSE)consisting of poly(vinylidene fluoride-hexafluoropropylene)(PVDF-HFP)-li_(6.5)La_(3)Zr_(1.5)Ta_(0.5)O_(12)(LLZTO)-succinonitrile(SN)and li bis(trifluoromethylsulphonyl)imide(liTFSI).The PVDF-HFP-based porous matrix made by electrospinning ensures good mechanical properties of the electrolyte membrane,and the large proportion of SN filling material makes the electrolyte membrane have an ionic conductivity of 1.11 mS·cm^(–1)without the addition of liquid electrolyte.The symmetric battery assembled with CSE can be cycled stably for more than 600 h,and the liFePO4|CSE|li full battery can also be cycled stably for more than 200 cycles.In addition to li metal batteries,li-O_(2)and li-CO_(2)batteries that use CSE as electrolytes also have good performances,reflecting the universality of CSE.CSE does not only guarantee good mechanical properties but also obtain a high ionic conductivity.This design provides a new idea for the commercial application of polymer-based solid batteries.

关键词： li metal batteries composite solid-state electrolyte succinonitrile ionic conductivity mechanical strength