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Electrochemical inactivation of cyanobacteria and microcystin degradation using a boron-doped diamond anode-A potential tool for cyanobacterial bloom control

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Journal of Environmental Sciences 2017年第3期29卷 248-261页

作者： Andrej Meglic Anja Pecman Tinkara Rozina Domen Lestan bojan Sedmak Arhel Ltd. Pustourhoua c.63SI-1000 LjubljanaSlovenia Centre for Soil and Environmental Sciences Biotechnical FacultyUniversity of LjubljanaJamnikarjeva 101SI-1000 LjubljanaSlovenia Envit Ltd. Vojkova c.63SI-1000 LjubljanaSlovenia Department of Genetic Toxicology and Cancer Biology National Institute of BiologyVecna pot 111SI-1000 LjubljanaSlovenia

Cyanobacterial blooms are global phenomena that can occur in calm and nutrient-rich （eutrophic） fresh and marine waters. Human exposure to cyanobacteria and their biologically active products is possible during water sports and various water activities, or by ingestion of contaminated water. Although the vast majority of harmful cyanobacterial products are confined to the interior of the cells, these are eventually released into the surrounding water following natural or artificially induced cell death. Electrochemical oxidation has been used here to damage cyanobacteria to halt their proliferation, and for microcystin degradation under in-vitro conditions. Partially spent Jaworski growth medium with no addition of supporting electrolytes was used. Electrochemical treatment resulted in the cyanobacterial loss of cell-buoyancy regulation, cell proliferation arrest, and eventual cell death. Microcystin degradation was studied separately in two basic modes of treatment： batch-wise flow, and constant flow, for electrolytic-cell exposure. Batch-wise exposure simulates treatment under environmental conditions, while constant flow is more appropriate for the study of boron-doped diamond electrode efficacy under laboratory conditions. The effectiveness of microcystin degradation was established using high-performance liquid chromatography-photodiode array detector analysis, while the biological activities of the products were estimated using a colorimetric protein phosphatase-1 inhibition assay. The results indicate potential for the application of electro-oxidation methods for the control of bloom events by taking advantage of specific intrinsic ecological characteristics of bloom-forming cyanobacteria. The applicability of the use of boron-doped diamond electrodes in remediation of water exposed to cyanobacteria bloom events is discussed.

关键词： Cyanobacterial bloom controlBoron-doped diamond anodeElectro-oxidationMicrocystin degradationProtein phosphatase-1 inhibitionWater remediation

Exploring the sublethal genotoxic effects of class II organophosphorus insecticide quinalphos on freshwater fish Cyprinus carpio

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Journal of Oceanology and Limnology 2021年第2期39卷 661-670页

作者： Devan HEMALATHA bojan NATARAJ Basuvannan RANGASAMY Kannan MAHARAJAN Mathan RAMESH Unit of Toxicology Department of ZoologySchool of Life SciencesBharathiar UniversityCoimbatore-641046Tamil NaduIndia Department of Zoology PSG College of Arts&ScienceCoimbatore-641014Tamil NaduIndia

The genotoxic effects of quinalphos(QP)were evaluated in the erythrocytes of Cyprinus carpio using the comet,micronucleus,and nuclear abnormality *** 96 h LC_(50) value of quinalphos,estimated by static renewal system,was 10.9µL/*** the basis of the LC_(50) value,two sublethal concentrations(1.09 and 2.18µL/L)of the compound were used together with cyclophosphamide(5 mg/L)as the positive *** fish were exposed for 35 d,and sampling was done at the end of 7,14,21,28,and 35 *** results reveal a significant dose-dependent increase in the frequencies of DNA strand breaks,micronucleus,and nuclear *** responses were also observed in fish exposed to the positive genotoxin *** collective findings demonstrated that quinalphos exerts a genotoxic effect on *** results further confirmed that the comet,micronucleus,and nuclear abnormality assays are useful tools in determining the potential genotoxicity of pesticides towards aquatic organisms.

关键词： quinalphos(QP) micronucleus nuclear abnormalities cyclophosphamide Cyprinus carpio

Toxicity assessment of polyethylene microplastics in combination with a mix of emerging pollutants on Physalaemus cuvieri tadpoles

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Journal of Environmental Sciences 2023年第5期127卷 465-482页

作者： Amanda Pereira da Costa Araújo Thiarlen Marinho da Luz Mohamed Ahmed Ibrahim Ahmed Mir Mohammad Ali Md.Mostafizur Rahman bojan Nataraj Daniela de Melo e Silva DamiàBarceló Guilherme Malafaia Post-Graduation Program in Environmental Sciences Federal University of GoiásGoianiaGO 74690-970Brazil Laboratory of Toxicology Applied to the Environment Goiano Federal InstituteUrutaíGO 75790-000Brazil Plant Protection Department Faculty of AgricultureAssiut UniversityAssiut 71526Egypt Department of Aquaculture Faculty of FisheriesAquaculture&Marine ScienceSher-e-Bangla Agricultural UniversityDhaka 1207Bangladesh Department of Environmental Sciences Jahangirnagar UniversitySavar 1342Bangladesh Unit of Toxicology Department of ZoologySchool of Life SciencesBharathiar UniversityCoimbatore 641046India Laboratory of Environmental Mutagenesis Federal University of GoiásGoianiaGO 74690-970Brazil Catalan Institute for Water Research(ICRA-CERCA) H_(2)O BuildingScientific and Technological Park of the University of GironaEmili Grahit 101Girona 17003Spain Water and Soil Quality Research Group Department of Environmental ChemistryInstitute of Environmental Assessment and Water Research(IDAEA-CSIC)JordiGirona 1826Barcelona 08034Spain Post-Graduation Program in Conservation of Cerrado Natural Resources Goiano Federal InstituteUrutaíGO 75790-000Brazil Post-Graduation Program in Ecology Conservationand BiodiversityFederal University of UberlandiaUberlandiaMG 38400-902Brazil Post-Graduation Programa in Biotechnology and Biodiversity Federal University of GoiásGoianiaGO 74605-050Brazil

Studies in recent years have shown that aquatic pollution by microplastics(MPs)can be considered to pose additional stress to amphibian ***,our knowledge of how MPs affect amphibians is very rudimentary,and even more limited is our understanding of their effects in combination with other emerging ***,we aimed to evaluate the possible toxicity of polyethylene MPs(PE-MPs)(alone or in combination with a mix of pollutants)on the health of Physalaemus cuvieri *** 30 days of exposure,multiple biomarkers were measured,including morphological,biometric,and developmental indices,behavioral parameters,mutagenicity,cytotoxicity,antioxidant and cholinesterase responses,as well as the uptake and accumulation of PE-MPs in *** on the results,there was no significant change in any of the parameters measured in tadpoles exposed to treatments,but induced stress was observed in tadpoles exposed to PE-MPs combined with themixture of pollutants,reflecting significant changes in physiological and biochemical *** principal component analysis(PCA)and integrated biomarker response(IBR)assessment,effects induced by pollutants in each test group were distinguished,confirming that the exposure of *** tadpoles to the PE-MPs in combination with a mix of emerging pollutants induces an enhanced stress response,although the uptake and accumulation of PE-MPs in these animalswas ***,our study provides newinsight into the danger to amphibians of MPs coexisting with other pollutants in aquatic environments.

关键词： Amphibians Environmental toxicology Micropollutants Emerging pollutants Aquatic pollution Biomarkers

Modified Helicobacter test using a new test meal and a 13C-urea breath test in Helicobacter pylori positive and negative dyspepsia patients on proton pump inhibitors

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World Journal of Gastroenterology 2017年第32期23卷 5954-5961页

作者： bojan Tepes Peter Malfertheiner Joachim Labenz Sitke Aygen AM DC Rogaska Prvomajska 29 A3250 Rogaska SlatinaSlovenia Universitatsklinikum Magdeburg A.o.R Klinik für Gastroenterologie Hepatologie und Infektiologie39120 MagdeburgGermany Department of Internal Medicine and Gastroenterology Diakonie KlinikumJung-Stilling Hospital Institut für Biomedizinische Analytik und NMR-Imaging GmbH(INFAI)

AIM To determine the sensitivity and specificity of the ^(13)C-urea breath test(UBT) in patients taking proton pump inhibitors(PPIs), using a new test meal Refex. METHODS One hundred and fourteen consecutive patients with dyspepsia, 53 Helicobacter pylori(H. pylori) positive, 49 H. pylori negative, were included in the study. The patients were then given esomeprazole 40 mg for 29 consecutive days, and the ^(13)C-UBT with the new test meal was performed the next morning. RESULTS The sensitivity of the ^(13)C-UBT with a cut off 2.5‰ was92.45%(95%CI: 81.79%-97.91%) by per-protocol(PP) analysis and 78.13 %(95%CI: 66.03%-87.49%) by intention-to-treat(ITT) analysis. The specificity of the ^(13)C-UBT test was 96.00 % in the ITT population(95%CI: 86.29%-99.51%) and 97.96% in the PP population(95%CI: 89.15%-99.95%).CONCLUSION The new test meal based ^(13)C-UBT is highly accurate in patients on PPIs and can be used in those unable to stop their PPI treatment.

关键词： Urea breath test new test meal Proton pump inhibitors Prospective randomized clinical trial Helicobacter pylori

Reliability Analysis of Piled Raft Foundation Using a Novel Hybrid Approach of ANN and Equilibrium Optimizer

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Computer Modeling in Engineering & Sciences 2021年第9期128卷 1033-1067页

作者： Abidhan Bardhan Priyadip Manna Vinay Kumar Avijit Burman bojan Zlender Pijush Samui Department of Civil Engineering National Institute of TechnologyPatnaBiharIndia Faculty of Civil Engineering Transportation Engineering and ArchitectureUniversity of MariborMariborSlovenia

In many civil engineering projects,Piled Raft Foundations(PRFs)are usually preferred where the incoming load fromthe superstructures is very *** geotechnical engineering practice,the settlement of soil layers is a critical issue for the serviceability of the ***,assessment of risk associated with the structures corresponding to the maximum allowable settlement of soils needs to be carried out in the design *** this study,reliability analysis of PRF based on settlement criteria is performed using a high-performance hybrid soft computing *** new approach is an integration of the artificial neural network(ANN)and a recently developed meta-heuristic algorithm called equilibrium optimizer(EO).The concept of reliability index was used to explore the feasibility of a newly constructed hybrid model of ANN and EO(i.e.,ANN-EO)against the conventional approach of calculating the probability of failure of *** results show that the proposed ANN-EO attained the most accurate prediction with R^(2)=0.9914 and RMSE=0.0518 in the testing phase,which are significantly better than those obtained from conventional ANN,multivariate adaptive regression splines,and genetic programming,including the ANNoptimized with particle swarmoptimization developed in this *** on the experimental results of different settlement values,the newly constructedANN-EOis very potential to analyze the risk associatedwith civil engineering ***,the present study would significantly contribute to the knowledge pool of reliability studies related to piled raft systems because the works of literature on reliability analysis of piled raft systems are relatively scarce.

关键词： Risk analysis soil meta-heuristic optimization particle swarm optimization

The effects of electrochemical oxidation on in-vivo fluorescence and toxin content in Microcystis aeruginosa culture

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Journal of Oceanology and Limnology 2018年第4期36卷 1091-1102页

作者： ROZINA Tinkara ELERSEK Tina Maja ZUPANCIC JUSTIN MEGLIC Andrej LESTAN Domen SEDMAK bojan Envit d.o.o. Vojkova cesta 63 1000 Ljubljana Slovenia Department of Genetic Toxicology and Cancer Biology National Institute of Biology Vecna pot 111 1000 Ljubljana Slovenia Arhel d.o.o. Pustovrhova ulica 15 1000 Ljubljana Slovenia Biotechnical Faculty University of Ljubljana Vecna pot 111 1000 Ljubljana Centre for Soil and Environmental Sciences Biotechnical Faculty University of Ljubljana Jamnikarjeva 101 1000 Ljubljana Slovenia

The increasing occurrence of cyanobacterial blooms in water bodies is a serious threat to the environment. Efficient in-lake treatment methods for the control of cyanobacteria proliferation are needed, their in-vivo detection to obtain a real-time response to their presence, as well as the information about their physiological state after the applied treatment. In-vivo fluorescence measurements of photosynthetic pigments have proved to be effective for quantitative and qualitative detection of phytoplankton in a water environment. In the experiment, chlorophyll and phycocyanin fluorescence sensors were used concurrently to detect stress caused by electrochemical oxidation applying an electrolytic cell equipped with borondoped diamond electrodes on a laboratory culture of cyanobacteria Microcystis aeruginosa PCC 7806. The inflicted injuries were reflected in a clear transient increase in the phycocyanin fluorescence signal(for 104 %? 43%) 24 h after the treatment, which was not the case for the chlorophyll fluorescence signal. In the next 72 h of observation, the fluorescence signals decreased(on 40% of the starting signal) indicating a reduction of cell number, which was confirmed by cell count(24% reduction of the starting concentration) and analysis of extracted chlorophyll and phycocyanin pigment. These results demonstrate the viability of the combined application of two sensors as a useful tool for in-vivo detection of induced stress, providing real-time information needed for the evaluation of the efficiency of the in-lake treatment and decision upon the necessity of its repetition. The electrochemical treatment also resulted in a lower free microcystins concentration compared to control.

关键词：水体蓝藻环境保护环境管理

Optical properties of titanium oxide films obtained by cathodic arc plasma deposition

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Plasma Science and Technology 2017年第12期19卷 111-118页

作者： Vukoman JOKANOVIC Bozana COLOVIC Anka TRAJKOVSKA PETKOSKA Ana MRAKOVIC bojan JOKANOVIC Milo NENADOVIC Manuela FERRARA llija NASOV Vinca Institute of Nuclear Sciences University of BelgradeMike Petrovica Alasa 12-1411001 BelgradeSerbia ALBOS Doo Orahovacka 1911000 BelgradeSerbia University St Kliment Ohridski Faculty of Technology and Technical SciencesVelesR.Macedonia4 GL groupWemer-von-Siemens-Straβe86405 MetingenGermany GL group Wemer-von-Siemens-Straβe86405 MetingenGermany TE-STT-SCIS Laboratorio Sviluppo Componenti ed Impianti SolariPiazzale Enrico Fermi 180055Porfici(NA)Italia Plasma Doo 29 November 66/91000 SkopjeR.Macedonia

Structural and optical properties of nanometric titanium oxide（TixOy） films obtained by cathodic arc plasma deposition were investigated. Phase analysis by x-ray diffraction and Fouriertransform infrared spectroscopy showed the presence of anatase, rutile, Ti_2O_3, Ti_4O_7 and amorphous phases. Scanning electron microscopy images showed well-developed surface morphology with nano-patterns. Spectroscopic ellipsometry revealed film thicknesses of 53 and50 nm, variable refractive indices dependent on the light wavelength and close to zero extinction coefficients for wavelengths higher than 500 nm. On the basis of ultraviolet–visible spectroscopy data and using the Tauc equation, band gap values for direct and indirect electron transitions were determined.

关键词： optical materials plasma deposition thin films ellipsometry

Study on spiral winding swimming motion control of a slender legless creature model

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High Technology Letters 2021年第4期27卷 415-421页

作者：卢振利 Ma Zhipeng Marko Pencic Maja Cavic Borovac Branislav Roumiana Ilieva bojan Nemec Marjan Mernik School of Electrical Engineering and Automation Changshu Institute of TechnologyChangshu 215500P.R.China School of Information and Control Engineering China University of Mining and TechnologyXuzhou 221116P.R.China Faculty of Technical Sciences University of Novi SadNovi Sad 621000Serbia Faculty of Management Technical University of SofiaSofia 1000Bulgaria Department of Automatics Biocybernetics and RoboticsJožef Stefan InstituteLjubljana 1000Slovenia Faculty of Electrical Engineering and Computer Science University of MariborMaribor 462000Slovenia

Through the observation and analysis of the motion trajectory of spiral winding motion for slender legless biological creatures in water,V-REP software is adopted to build a dynamic simulator to study on the mechanism of spiral winding swimming(SWS)*** using the output of spiral function(SF),the dynamic simulation model of slender legless creature(SLC)realizes the SWS motion in *** corresponding experiments under the control of different bending angle of SF to control the dynamic model are also carried out to analyze the water performance of SWS in still *** with the output of two different bending angles of the spiral function,the dynamic model can be used to realize SWS and up/down *** work provides technical reserve and experimental platform for the corresponding study in related fields.

关键词： V-REP spiral winding swimming(SWS) motion control slender legless creature(SLC)

Exploring the sublethal genotoxic effects of class Ⅱorganophosphorus insecticide quinalphos on freshwater fish Cyprinus carpio

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Journal of Oceanology and Limnology 2021年第2期39卷 661-671页

作者： Devan HEMALATHA bojan NATARAJ Basuvannan RANGASAMY Kannan MAHARAJAN Mathan RAMESH Unit of Toxicology Department of Zoology School of Life Sciences Bharathiar University Department of Zoology PSG College of Arts & Science

The genotoxic effects of quinalpho s(QP) were evaluated in the erythrocyte s of Cyprinus carpio using the comet,micronucleus,and nuclear abnormality *** 96 h LCvalue of quinalpho s,estimated by static renewal system,was 10.9 μL/*** the basis of the LCvalue,two sublethal concentrations(1.09 and 2.18 μL/L) of the compound were used together with cyclophosphamide(5 mg/L) as the positive *** fish were exposed for 35 d,and sampling was done at the end of 7,14,21,28,and 35 *** re sults reveal a significant dose-dependent increase in the frequencies of DNA strand breaks,micronucleus,and nuclear *** responses were also observed in fish exposed to the positive genotoxin *** collective findings demonstrated that quinalphos exerts a genotoxic effect on *** results further confirmed that the comet,micronucleus,and nuclear abnormality assays are useful tools in determining the potential genotoxicity of pesticides towards aquatic organisms.

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