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DNA Markers for Selection of Late Blight Resistant Potato Breeding Lines

American Journal of Plant Sciences 2017年第6期8卷 1197-1209页

作者： Shaohua Chen Tudor Borza bohyun byun Robert Coffin Joyce Coffin Rick Peters Gefu Wang-Pruski Department of Plant Food and Environmental Sciences Faculty of Agriculture Dalhousie University Truro Canada Privar Farm Inc. North Wiltshire Canada Agriculture and Agri-Food Canada Charlottetown Research and Development Centre Charlottetown Canada Fujian Agriculture and Forestry University Fuzhou China

Potato late blight, caused by the oomycete Phytophthora infestans, is one of the most devastating diseases in the agricultural sector around the world. Many genes (R genes) conferring resistance to late blight have been identified in various potato species and most of these R genes have been used in potato breeding. The aim of this study was to develop and validate PCR-based assays for the R genes Rpi-blb1, Rpi-blb2, Rpi-blb3 and Rpi-bt1, to distinguish between late blight resistant and late blight susceptible potato progeny in the given breeding background. A total of 100 breeding progeny were screened for the presence of these R genes and tested for resistance against P. infestans mating type A2, genotype US-8 strain, using detached leaf and tuber rot assays. PCR products for the Rpi-blb1 and Rpi-bt1 resistance genes were identified in the resistant progeny but were absent in the susceptible ones;therefore these PCR assays could differentiate between late blight resistant and susceptible plants. Genotypic data from the DNA markers derived from the Rpi-blb1 and Rpi-bt1 genes was found to correlate with the phenotypic data for foliar late blight but not with data for tuber rot. Our results demonstrate that markers derived from these two R genes could be useful for marker-assisted selection (MAS) for foliar late blight resistance in potato breeding programs.

关键词： Late Blight Phytophthora infestans Resistance R genes DNA Markers

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Ethyl acetate fraction of Sargassum pallidum extract attenuates particulate matter-induced oxidative stress and inflammation in keratinocytes and zebrafish

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Asian Pacific Journal of Tropical Biomedicine 2024年第4期14卷 137-146页

作者： Wook Chul Kim Ji-Won Park bohyun Yun WonWoo Lee Kyung-Min Choi Seung-Hong Lee Department of Medical Science Soonchunhyang UniversityAsan 31538Republic of Korea Honam National Institute of Biological Resources 99Gohadoan-gilMokpo-siJeollanam-do 58762Republic of Korea Department of Pharmaceutical Engineering Soonchunhyang UniversityAsan 31538Republic of Korea

Objective:To evaluate the effect of the ethyl acetate fraction derived from Sargassum pallidum extract against particulate matter(PM)-induced oxidative stress and inflammation in HaCaT cells and ***:HaCaT cells and zebrafish were used to evaluate the protective effects of the ethyl acetate fraction of Sargassum pallidum extract against PM-induced oxidative stress and *** production of nitric oxide(NO),intracellular ROS,prostaglandin E_(2)(PGE_(2)),and pro-inflammatory cytokines,and the expression levels of COX-2,iNOS,and NF-κB were evaluated in PM-induced HaCaT ***,the levels of ROS,NO,and lipid peroxidation were assessed in the PM-exposed zebrafish ***:The ethyl acetate fraction of Sargassum pallidum extract significantly decreased the production of NO,intracellular ROS,and PGE_(2) in PM-induced HaCaT *** addition,the fraction markedly suppressed the levels of pro-inflammatory cytokines and inhibited the expression levels of COX-2,iNOS,and NF-κ***,it displayed remarkable protective effects against PM-induced inflammatory response and oxidative stress,represented by the reduction of NO,ROS,and lipid peroxidation in ***:The ethyl acetate fraction of Sargassum pallidum extract exhibits a protective effect against PM-induced oxidative stress and inflammation both in vitro and in vivo and has the potential as a candidate for the development of pharmaceutical and cosmeceutical products.

关键词： Particulate matter Inflammation Oxidative stress Sargassum pallidum Ethyl acetate fraction Zebrafish

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Catalpa bignonioides extract improves exercise performance through regulation of growth and metabolism in skeletal muscles

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Asian Pacific Journal of Tropical Biomedicine 2024年第2期14卷 47-54页

作者： Hoibin Jeong Dong-joo Lee Sung-Pil Kwon SeonJu Park Song-Rae Kim Seung Hyun Kim Jae-Il Park Deug-chan Lee Kyung-Min Choi WonWoo Lee Ji-Won Park bohyun Yun Su-Hyeon Cho Kil-Nam Kim Seoul Center Korea Basic Science InstituteSeoul 02841Republic of Korea Chuncheon Center Korea Basic Science InstituteChuncheon 24341Republic of Korea Research&Development Center Chungdam CDC JNPharm LLC.Chuncheon 24341Republic of Korea Department of Biomedical Technology Kangwon National UniversityChuncheon 24341Republic of Korea Yonsei Institute of Pharmaceutical Sciences College of PharmacyYonsei UniversityIncheon 21983Republic of Korea Gwangju Center Korea Basic Science InstituteGwangju 61751Republic of Korea Honam National Institute of Biological Resources 99Gohadoan-gilMokpo-siJeollanam-do58762Republic of Korea Department of Bio-analysis Science University of Science&TechnologyDaejeon34113Republic of Korea

Objective:To evaluate the effects of Catalpa bignonioides fruit extract on the promotion of muscle growth and muscular capacity in vitro and in ***:Cell viability was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide *** proliferation was assessed using a 5-bromo-2’-deoxyuridine(BrdU)assay *** blot analysis was performed to determine the protein expressions of related *** effects of Catalpa bignonioides extract were investigated in mice using the treadmill exhaustion test and whole-limb grip strength *** composition analysis was performed using high-performance liquid chromatography(HPLC).Results:Catalpa bignonioides extract increased the proliferation of C2C12 mouse myoblasts by activating the Akt/mTOR signaling *** also induced metabolic changes,increasing the number of mitochondria and glucose metabolism by phosphorylating adenosine monophosphate-activated protein *** an in vivo study,the extract-treated mice showed improved motor abilities,such as muscular endurance and grip ***,HPLC analysis showed that vanillic acid may be the main component of the Catalpa bignonioides extract that enhanced muscle ***:Catalpa bignonioides improves exercise performance through regulation of growth and metabolism in skeletal muscles,suggesting its potential as an effective natural agent for improving muscular strength.

关键词： Catalpa bignonioides Skeletal muscle Cell proliferation Mitochondria Energy metabolism C2C12

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Transarterial chemoembolization with drug-eluting beads in hepatocellular carcinoma

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World Journal of Gastroenterology 2016年第40期22卷 8853-8861页

作者： Hee Chul Nam bohyun Jang Myeong Jun Song Department of Internal Medicine College of Medicine the Catholic University of Korea Division of Hepatology Department of Internal Medicine Daejeon St. Mary’s Hospital College of Medicine The Catholic University of Korea

Transarterial chemoembolization(TACE) is a widely used standard treatment for patients with hepatocellular carcinoma(HCC) who are not suitable candidates for curative treatments. The rationale for TACE is that intra-arterial chemotherapy using lipiodol and chemotherapeutic agents, followed by selective vascular embolization, results in a strong cytotoxic effect as well as ischemia(conventional TACE). Recently, drugeluting beads(DC Beads?) have been developed for transcatheter treatment of HCC to deliver higher doses of the chemotherapeutic agent and to prolong contact time with the tumor. DC Beads? can actively sequester doxorubicin hydrochloride from solution and release it in a controlled sustained fashion. Treatment with DC Beads? substantially reduced the amount of chemotherapeutic agent that reached the systemic circulation compared with conventional, lipiodol-based regimens, significantly reducing drug-related adverse events. In this article, we describe the treatment response, survival, and safety of TACE used with drugeluting beads for the treatment of HCC and discuss future therapeutic possibilities.

关键词： Hepatocellular carcinoma Transarterial chemoembolization Conventional TACE Drug-eluting beads Treatment response

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海岸住区再发展工程

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建筑技艺 2009年第5期15卷 104-106页

作者： Charles Wee AIA Scott Callihan Michael Collins Andy Bae Brandon Sakuma Humberto Barraza Lingie Park Peter Lee Jae Young Jang Goon bohyun Soobok Park Sang Joon Park Ham ChulKim Kwang-Woo Yoo

本方案位于韩国釜山，场地面积469528平方英尺，其中地上部分342226平方英尺，地下部分127302平方英尺。该群落的建筑密度是21．9％，拥有9栋塔楼，最高处达到161米（52层）；共有2593个单元，4449个停车位，每个单元的尺度从700平方英... 详细信息

本方案位于韩国釜山，场地面积469528平方英尺，其中地上部分342226平方英尺，地下部分127302平方英尺。该群落的建筑密度是21．9％，拥有9栋塔楼，最高处达到161米（52层）；共有2593个单元，4449个停车位，每个单元的尺度从700平方英尺到2000平方英尺不等。

关键词：工程住区海岸地上部分地下部分建筑密度平方停车位

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