检索结果-南通市图书馆

Large animal models for Huntington's disease research

Zoological Research 2024年第2期45卷 275-283页

作者： bofeng han Weien Liang Xiao-Jiang Li Shihua Li Sen Yan Zhuchi Tu Guangdong-Hongkong-Macao CNS Regeneration Institute of Jinan University Key Laboratory of CNS Regeneration(Jinan University)-Ministry of EducationGuangdong Key Laboratory of Non-Human Primate ResearchGuangzhouGuangdong 510632China Department of Pathophysiology School of MedicineJinan UniversityGuangzhouGuangdong 510632China

Huntington'sdisease(HD)isahereditary neurodegenerative disorder for which there is currently no effectivetreatmentavailable.Consequently,the development of appropriate disease models is critical to thoroughly investigate disease progression.The genetic basis of HD involves the abnormal expansion of CAG repeats in the huntingtin(HTT)gene,leading to the expansion of a polyglutamine repeat in the HTT protein.Mutant HTT carrying the expanded polyglutamine repeat undergoes misfolding and forms aggregates in the brain,which precipitate selective neuronal loss in specific brain regions.Animal models play an important role in elucidating the pathogenesis of neurodegenerative disorders such as HD and in identifying potential therapeutic targets.Due to the marked species differences between rodents and larger animals,substantial efforts have been directed toward establishing large animal models for HD research.These models are pivotal for advancing the discovery of novel therapeutic targets,enhancing effective drug delivery methods,and improving treatment outcomes.We have explored the advantages of utilizing large animal models,particularly pigs,in previous reviews.Since then,however,significant progress has been made in developing more sophisticated animal models that faithfully replicate the typical pathology of HD.In the current review,we provide a comprehensive overview of large animal models of HD,incorporating recent findings regarding the establishment of HD knock-in(KI)pigs and their genetic therapy.We also explore the utilization of large animal models in HD research,with a focus on sheep,non-human primates(NHPs),and pigs.Our objective is to provide valuable insights into the application of these large animal models for the investigation and treatment of neurodegenerative disorders.

关键词： Huntington's disease Large animal models Sheep Non-human primates Transgenic pigs

来源：

维普期刊数据库

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

Tauopathy promotes spinal cord-dependent production of toxic amyloid-beta in transgenic monkeys

引用

Signal Transduction and Targeted Therapy 2023年第10期8卷 5023-5035页

作者： Zhuchi Tu Sen Yan bofeng han Cajuan Li Weien Liang Yingqi Lin Yongyan Ding Huiyi Wei Lu Wang Hao Xu Jianmeng Ye Bang Li Shihua Li Xiao-Jiang Li Guangdong Key Laboratory of Non-human Primate Research Key Laboratory of CNS Regeneration(Ministry of Education)GHM Institute of CNS RegenerationJinan UniversityGuangzhou 510632China Center of Cyclotron and PET Radiopharmaceuticals Department of Nuclear Medicine and PET/CT-MRI Centerthe First Affliated Hospital of Jinan UniversityGuangzhou 510630China Guangdong Landau Biotechnology Co.Ltd Guangzhou 510555China

Tauopathy,characterized by the hyperphosphorylation and accumulation of the microtubule-associated protein tau,and the accumulation of Aβ oligomers,constitute the major pathological hallmarks of Alzheimer's disease.However,the relationship and causal roles of these two pathological changes in neurodegeneration remain to be defined,even though they occur together or independently in several neurodegenerative diseases associated with cognitive and movement impairment.

关键词： pathological constitute marks

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Generation of inactivated IL2RG and RAG1 monkeys with severe combined immunodeficiency using base editing

引用

Signal Transduction and Targeted Therapy 2023年第10期8卷 4825-4837页

作者： Xiao Zheng Chunhui Huang Yingqi Lin bofeng han Yizhi Chen Caijuan Li Jiawei Li Yongyan Ding Xichen Song Wei Wang Weien Liang Jianhao Wu Jiaxi Wu Jiale Gao Chengxi Wei Xudong Zhang Zhuchi Tu Sen Yan Guangdong Key Laboratory of Non-human Primate Research Guangdong-Hongkong-Macao Institute of CNS RegenerationJinan University510632 GuangzhouChina Department of Pathophysiology School of MedicineJinan University510632 GuangzhouChina

Severe combined immunodeficiency(SCiD)encompasses a range of inherited disorders that lead to a profound deterioration of the immune system.Among the pivotal genes associated with SCID,RAG1 and IL2RG play crucial roles.IL2RG is essential for the development,differentiation,and functioning of T,B,and NK cells,while RAG1 critically contributes to adaptive immunity by facilitating V(D)J recombination during the maturation of lymphocytes.Animal models carrying mutations in these genes exhibit notable deficiencies in their immune systems.

关键词： immunodeficiency immunity RAG1

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

烟富8号苹果带袋采收和不套袋果实品质的比较

引用

中国果树 2023年第3期 26-28,34页

作者：徐钦臣韩博峰韩家宁聂继云原永兵贾东杰青岛农业大学园艺学院农业农村部果品质量安全风险评估实验室(青岛)全国名特优新农产品(园艺产品)全程质量控制技术青岛中心青岛市现代农业质量与安全工程重点实验室山东266109

比较了烟富8号苹果带袋采收和不套袋的果实品质和货架期品质变化。结果表明:烟富8号苹果带袋采收果实单果重和果形指数均高于不套袋果实,但差异均不显著;烟富8号带袋采收果实硬度、可溶性固形物含量和还原糖含量、果皮a*值、类胡萝卜素... 详细信息

比较了烟富8号苹果带袋采收和不套袋的果实品质和货架期品质变化。结果表明:烟富8号苹果带袋采收果实单果重和果形指数均高于不套袋果实,但差异均不显著;烟富8号带袋采收果实硬度、可溶性固形物含量和还原糖含量、果皮a*值、类胡萝卜素含量和花青苷含量、果皮果肉总黄酮含量等均显著低于不套袋果实;烟富8号带袋采收果实果皮L*、b*值和果实可滴定酸含量均显著高于不套袋果实;烟富8号带袋采收果实维生素C含量和类胡萝卜素含量与不套袋果实无显著差异。在货架期,烟富8号带袋采收果实硬度和还原糖含量、果皮类胡萝卜素含量和花青苷含量、果皮果肉总黄酮含量显著低于不套袋果实,烟富8号带袋采收果肉类胡萝卜素含量显著高于不套袋果实。

关键词：苹果烟富8号带袋采收不套袋果实品质货架期

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Genetic polymorphisms of 44 Y chromosomal genetic markers in the Inner Mongolia han population and its genetic relationship analysis with other reference populations

引用

Forensic Sciences Research 2022年第3期7卷 510-517页

作者： Xiaoye Jin Guohui Xing Chunhua Yang Xingru Zhang Wei Cui Chong Chen bofeng Zhu Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research College of StomatologyXi’an Jiaotong UniversityXi’anChina College of Forensic Science Xi’an Jiaotong University Health Science CenterXi’anChina Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases College of StomatologyXi’an Jiaotong UniversityXi’anChina People’s Hospital of Arong Banner Hulun Buir CityChina Multi-Omics Innovative Research Center of Forensic Identification Department of Forensic GeneticsSchool of Forensic MedicineSouthern Medical UniversityGuangzhouChina

Y chromosomal genetic markers in the non-recombining region are commonly used for human evolution research,familial searching,and forensic male differentiation since they strictly follow paternal inheritance.Y chromosomal short tandem repeats(Y-STRs)possess extraordinarily advantages in forensic applications because of their high polymorphisms and special genetic pattern.Here,we assessed the genetic diversities of 41 Y-STRs and three Y chromosomal insertion/deletion(Y-InDels)loci in the Chinese Inner Mongolia han population;besides,genetic differentiation analyses among the studied han population and other previously reported populations were conducted based on 27 same Y-STRs.Totally,425 alleles were observed in 324 Inner Mongolia han individuals for these Y-markers.Gene diversities of these Y-markers distributed from 0.0306 to 0.9634.The haplotype diversity and discriminatory capacity of these Y-markers in the Inner Mongolia han population were 0.9999 and 0.98457,respectively.Haplotype resolution comparisons of different Y-marker groups in the studied han population revealed that higher haplotype resolution could be achieved for these 44 Y-markers.Population genetic analyses of the Inner Mongolia han population and other reference populations demonstrated that the studied han population had relatively closer genetic affinities with Northern han Chinese populations than Southern han and other minority groups.To sum up,these 44 Y-markers can be utilized as a valuable tool for male differentiation in the Inner Mongolia han population.

关键词： Forensic sciences forensic genetics Y chromosomal STR InDel Inner Mongolia han male differentiation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genetic polymorphisms and phylogenetic analyses of the Ü-Tsang Tibetan from Lhasa based on 30 slowly and moderately mutated Y-STR loci

引用

Forensic Sciences Research 2022年第2期7卷 181-188页

作者： Jiuyang Ding Haoliang Fan Yongsong Zhou Zhuo Wang Xiao Wang Xuheng Song bofeng Zhu Pingming Qiu Shanghai Key Laboratory of Forensic Medicine Shanghai Forensic Service PlatformAcademy of Forensic ScienceShanghaiChina School of Forensic Medicine Southern Medical UniversityGuangzhouChina School of Basic Medicine and Life Science Hainan Medical UniversityHaikouChina Multi-Omics Innovative Research Center of Forensic Identification Department of Forensic GeneticsSchool of Forensic MedicineSouthern Medical UniversityGuangzhouChina Department of Psychiatry The First Clinical Medical CollegeShanxi Medical UniversityTaiyuanChina Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research College of StomatologyXi’an Jiaotong UniversityXi’anChina Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases College of StomatologyXi’an Jiaotong UniversityXi’anChina

As a result of the expansion of old Tibet on the Qinghai-Tibet Plateau,Tibetans diverged into three main branches,Ü-Tsang,Amdo,and Kham Tibetan.Ü-Tsang Tibetans are geographically distributed across the wide central and western portions of the Qinghai-Tibet Plateau while Lhasa is the central gathering place for Tibetan culture.The AGCU Y30,a 6-dye fluorescence kit including 30 slowly and moderately mutated Y-STR loci,has been validated for its stability and sensitivity in different biomaterials and diverse Chinese populations(han and other minorities),and widely used in the practical work of forensic science.However,the 30 Y-STR profiling of Tibetan,especially forÜ-Tsang Tibetan,were insufficient.We utilized the AGCU Y30 to genotype 577Ü-Tsang Tibetan unrelated males from Lhasa in the Tibet Autonomous Region of China to fill up the full and accurate Y-STR profiles.A total of 552 haplotypes were observed,536(97.10%)of which were unique.One hundred and ninety-four alleles were observed at 26 single copy loci and the allelic frequencies ranged from 0.0017 to 0.8180.For the two multi-copy loci DYS385a/b and DYS527a/b,64 and 36 allelic combinations were observed,respectively.The gene diversity(GD)values ranged from 0.3079 at DYS391 to 0.9142 at DYS385a/b and the overall haplotype diversity(HD)was 0.9998,and its discrimination capacity(DC)was 0.9567.The population genetic analyses demonstrated that LhasaÜ-Tsang Tibetan had close relationships with other Tibetan populations from Tibet and Qinghai,especially withÜ-Tsang Tibetan.From the perspective of Y haplogroups,the admixture of the southward Qiang people with dominant haplogroup O-M122 and the northward migrations of the initial settlers of East Asia with haplogroup D-M175 hinted the Sino-Tibetan homologous,thus,we could not ignore the gene flows with other Sino-Tibetan populations,especially for han Chinese,to characterize the forensic genetic landscape of Tibetan.

关键词： Forensic sciences forensic genetics Y-STR Ü-Tsang Tibetan phylogenetic analysis AGCU Y30 O-M122

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genetic diversity analysis of forty-three insertion/deletion loci for forensic individual identification in han Chinese from Beijing based on a novel panel

引用

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2022年第3期23卷 241-248页

作者： Congying ZHAO Jinlong YANG Hui XU Shuyan MEI Yating FANG Qiong LAN Yajun DENG bofeng ZHU Guangzhou Key Laboratory of Forensic Multi-Omics for Precision Identification School of Forensic MedicineSouthern Medical UniversityGuangzhou510515China Beijing Zhongzheng DNA Evidence Institute Beijing101318China Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research College of StomatologyXi’an Jiaotong UniversityXi’an710004China

Due to the virtues of no stutter peaks,low rates of mutation,and short amplicon sizes,insertion/deletion(InDei)polymorphism is an indispensable tool for analyzing degraded DNA samples from crime scenes for human identifications(Wang et al.,2021).Herein,a self-developed panel of 43 InDei loci constructed previously by our group was utilized to evaluate the genetic diversities and explore the genetic background of the han Chinese from Beijing(HCB)including 301 random healthy individuals.

关键词： insertion utilized sizes

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

An interpretation of the genetic polymorphism and population genetic background of Ankang han population via a novel InDel panel

引用

Forensic Sciences Research 2022年第4期7卷 694-701页

作者： Wei Cui Xiaoye Jin Yating Fang Qiong Lan Jiangwei Lan Man Chen Shuyan Mei Tong Xie bofeng Zhu Multi-Omics Innovative Research Center of Forensic Identification Department of Forensic Genetics School of Forensic MedicineSouthern Medical UniversityGuangzhouChina Department of Forensic Medicine Guizhou Medical UniversityGuiyangChina Key laboratory of Shaanxi Province for Craniofacial Precision Medicine ResearchCollege of StomatologyXi’an Jiaotong UniversityXi’anChina

In this research,genotyping data of 43 InDel loci in 311 han individuals in Ankang City,Shaanxi Province,China were detected using a self-developed five-dye multiplex amplification panel.The allelic frequencies and forensic parameters of all InDel loci were calculated.The combined power of discrimination and probability of exclusion values were 0.99999999999999999882739 and 0.999887424,respectively,which demonstrated that this 43-InDel panel was powerful for individual identifications in Ankang han population.Moreover,genetic distances,pairwise F_(ST)values,principal component analyses,phylogenetic trees and STRUCTURE analyses were performed to investigate the genetic affinities between Ankang han and reference groups.Population genetic investigations indicated that Ankang han population had a close genetic relationship with Southern han population compared with other reference groups.

关键词： Forensic sciences forensic genetics forensic parameter InDel Population genetics Ankang han population

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genetic and structural characterization of 20 autosomal short tandem repeats in the Chinese Qinghai han population and its genetic relationships and interpopulation differentiations with other reference populations

引用

Forensic Sciences Research 2018年第2期3卷 145-152页

作者： Zhanhai Wang Bin Lu Xiaoye Jin Jiangwei Yan Haotian Meng bofeng Zhu The Public Security Bureau of Qinghai Province XiningChina Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research College of StomatologyXi'an Jiaotong UniversityXi'anChina Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases College of StomatologyXi'an Jiaotong UniversityXi'anChina Research Center of Stomatology Stomatological HospitalXi'an Jiaotong UniversityXi'anChina Beijing Institute of Genomics Chinese Academy of SciencesBeijingChina

China is a multinational country composed of 56 ethnic groups of which the han Chinese accounts for 91.60%.Qinghai Province is located in the northeastern part of the Qinghai-Tibet Plateau,has an area of 72.12 km2,and is the fourth largest province in China.In the present study,we investigated the genetic polymorphisms of 20 short tandem repeat (STR) loci in a Qinghai han population,as well as its genetic relationships with other populations.A total of 273 alleles were identified in 2 000 individuals at 20 loci,and the allelic frequency ranged from 0.0002 to 0.5327.The 20 STR loci showed a relatively high polymorphic rate in the studied group.Observed and expected heterozygosities ranged 0.613 0-0.907 5 and 0.614 8-0.920 0,respectively.The combined power of discrimination,and the probability of exclusion in duo and trio cases were 0.999 999 999 999 999 999 999 999 34,0.9999960 and 0.9999999965,respectively.Analyses of interpopulation differentiation revealed that the most significant differences were found between the Qinghai han and Malaysian,while no significant differences were found between the Qinghai han and han people from Shaanxi and Jiangsu.The results of principal component analysis,multidimensional scaling analysis and phylogenetic reconstructions also suggested the close relationships between the Qinghai han and other two han populations.The present results,therefore,indicated that these 20 STR loci could be used for paternity testing and individual identification in forensic applications,and may also provide information for the studies of genetic relationships between Qinghai han and other groups.

关键词： Genetic polymorphisms forensic genetics phylogenetic reconstruction short tandem repeat

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

1kW家用SOFC-CHP系统建模及性能分析

引用

太阳能学报 2011年第4期32卷 604-610页

作者：徐晗党政白博峰西安交通大学建筑环境与设备工程系西安710049 西安交通大学能源与动力工程学院西安710049

构建一个以天然气为燃料的SOFC-CHP系统,推导SOFC传热传质及电化学方程,建立各个组件的数学模型,编写计算程序,对发电功率为1kW的家用SOFC-CHP系统在设计工况下进行性能模拟并探讨不同系统参数对性能的影响。计算结果表明:在设计工况下... 详细信息

构建一个以天然气为燃料的SOFC-CHP系统,推导SOFC传热传质及电化学方程,建立各个组件的数学模型,编写计算程序,对发电功率为1kW的家用SOFC-CHP系统在设计工况下进行性能模拟并探讨不同系统参数对性能的影响。计算结果表明:在设计工况下,系统热电联供效率远高于电池单独发电的效率;此外,随着燃料入口流量的增大,系统发电功率存在一个最大值,燃料利用率与发电效率不断减小,系统热电联供效率不断增大,SOFC的温度梯度分布则越来越平缓;同时发现降低过量空气系数可以提高该CHP系统的性能。

关键词：固体氧化物燃料电池热电联供性能分析效率

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：