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AAV2-PDE6B restores retinal structure and function in the retinal degeneration 10 mouse model of retinitis pigmentosa by promoting phototransduction and inhibiting apoptosis

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Neural Regeneration Research 2025年第8期20卷 2408-2419页

作者： Ruiqi Qiu Mingzhu yang Xiuxiu Jin Jingyang Liu Weiping Wang Xiaoli Zhang Jinfeng Han bo lei Henan Eye Hospital Henan Provincial People’s HospitalPeople’s Hospital of Zhengzhou UniversityZhengzhouHenan ProvinceChina Eye Institute Henan Academy of Innovations in Medical ScienceZhengzhouHenan ProvinceChina Branch of National Clinical Research Center for Ocular Disease Henan Provincial People’s HospitalZhengzhouHenan ProvinceChina Academy of Medical Science Zhengzhou UniversityZhengzhouHenan ProvinceChina

Retinitis pigmentosa is a group of inherited diseases that lead to retinal degeneration and photoreceptor cell ***,there is no effective treatment for retinitis pigmentosa caused by PDE6B ***-associated virus(AAV)-mediated gene therapy is a promising strategy for treating retinitis *** aim of this study was to explore the molecular mechanisms by which AAV2-PDE6B rescues retinal *** do this,we injected retinal degeneration 10(rd10)mice subretinally with AAV2-PDE6B and assessed the therapeutic effects on retinal function and structure using dark-and light-adapted electroretinogram,optical coherence tomography,and ***-independent acquisition-mass spectrometry-based proteomic analysis was conducted to investigate protein expression levels and pathway enrichment,and the results from this analysis were verified by real-time polymerase chain reaction and western ***2-PDE6B injection significantly upregulated PDE6βexpression,preserved electroretinogram responses,and preserved outer nuclear layer thickness in rd10 *** expressed proteins between wild-type and rd10 mice were closely related to visual perception,and treating rd10 mice with AAV2-PDE6B restored differentially expressed protein expression to levels similar to those seen in wild-type *** Encyclopedia of Genes and Genome analysis showed that the differentially expressed proteins whose expression was most significantly altered by AAV2-PDE6B injection were enriched in phototransduction ***,the phototransductionrelated proteins Pde6α,Rom1,Rho,Aldh1a1,and Rbp1 exhibited opposite expression patterns in rd10 mice with or without AAV2-PDE6B ***,Bax/Bcl-2,p-ERK/ERK,and p-c-Fos/c-Fos expression levels decreased in rd10 mice following AAV2-PDE6B *** data suggest that AAV2-PDE6B-mediated gene therapy promotes phototransduction and inhibits apoptosis by inhibiting the ERK signaling pathway and upr

关键词： apoptosis AAV2-PDE6B ERK1/2 gene therapy phototransduction proteomics rd10 retinitis pigmentosa

The potential mechanism and clinical application value of remote ischemic conditioning in stroke

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Neural Regeneration Research 2025年第6期20卷 1613-1627页

作者： yajun Zhu Xiaoguo Li Xingwei lei Liuyang Tang Daochen Wen bo Zeng Xiaofeng Zhang Zichao Huang Zongduo Guo The First Affiliated Hospital of Chongqing Medical University ChongqingChina

Some studies have confirmed the neuroprotective effect of remote ischemic conditioning against stroke. Although numerous animal researches have shown that the neuroprotective effect of remote ischemic conditioning may be related to neuroinflammation, cellular immunity, apoptosis, and autophagy, the exact underlying molecular mechanisms are unclear. This review summarizes the current status of different types of remote ischemic conditioning methods in animal and clinical studies and analyzes their commonalities and differences in neuroprotective mechanisms and signaling pathways. Remote ischemic conditioning has emerged as a potential therapeutic approach for improving stroke-induced brain injury owing to its simplicity, non-invasiveness, safety, and patient tolerability. Different forms of remote ischemic conditioning exhibit distinct intervention patterns, timing, and application range. Mechanistically, remote ischemic conditioning can exert neuroprotective effects by activating the Notch1/phosphatidylinositol 3-kinase/Akt signaling pathway, improving cerebral perfusion, suppressing neuroinflammation, inhibiting cell apoptosis, activating autophagy, and promoting neural regeneration. While remote ischemic conditioning has shown potential in improving stroke outcomes, its full clinical translation has not yet been achieved.

关键词： Akt apoptosis autophagy cerebral perfusion cerebral vascular stenosis clinical transformation hemorrhagic stroke ischemic stroke neuroinflammation neuroprotection Notch1 PI3K remote ischemic conditioning stroke

Human-induced pluripotent stem cell-derived neural stem cell exosomes improve blood-brain barrier function after intracerebral hemorrhage by activating astrocytes via PI3K/AKT/MCP-1 axis

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Neural Regeneration Research 2025年第2期20卷 518-532页

作者： Conglin Wang Fangyuan Cheng Zhaoli Han bo yan Pan Liao Zhenyu Yin Xintong Ge Dai Li Rongrong Zhong Qiang Liu Fanglian Chen Ping lei Department of Geriatrics Tianjin Medical University General HospitalTianjinChina School of Medicine Nankai UniversityTianjinChina Tianjin Neurological Institute TianjinChina

Cerebral edema caused by blood-brain barrier injury after intracerebral hemorrhage is an important factor leading to poor ***-induced pluripotent stem cell-derived neural stem cell exosomes(hiPSC-NSC-Exos)have shown potential for brain injury repair in central nervous system *** this study,we explored the impact of hiPSC-NSC-Exos on blood-brain barrier preservation and the underlying *** results indicated that intranasal delivery of hiPSC-NSC-Exos mitigated neurological deficits,enhanced blood-brain barrier integrity,and reduced leukocyte infiltration in a mouse model of intracerebral ***,hiPSC-NSC-Exos decreased immune cell infiltration,activated astrocytes,and decreased the secretion of inflammatory cytokines like monocyte chemoattractant protein-1,macrophage inflammatory protein-1α,and tumor necrosis factor-αpost-intracerebral hemorrhage,thereby improving the inflammatory *** sequencing indicated that hiPSC-NSC-Exo activated the PI3K/AKT signaling pathway in astrocytes and decreased monocyte chemoattractant protein-1 secretion,thereby improving blood-brain barrier *** with the PI3K/AKT inhibitor LY294002 or the monocyte chemoattractant protein-1 neutralizing agent C1142 abolished these *** summary,our findings suggest that hiPSC-NSC-Exos maintains blood-brain barrier integrity,in part by downregulating monocyte chemoattractant protein-1 secretion through activation of the PI3K/AKT signaling pathway in astrocytes.

关键词： AKT astrocyte blood-brain barrier cerebral edema exosomes human-induced pluripotent stem cells intracerebral hemorrhage neural stem cells neuroinflammation PI3K

Significantly enhanced of tungsten diselenide functionalization optoelectronic performance phototransistor via surface

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Nano Research 2017年第4期10卷 1282-1291页

作者： bo lei Department of Physics National University of Singapore Singapore 117542 Singapore 2 Centre forAdvanced D Materials and Graphene Research Centre National University of Singapore Singapore 117546 Singapore Department of Chemistry National University of Singapore Singapore 117543 Singapore SZU-NUS Collaborative Innovation Center for Optoelectronic Science and Technology Shenzhen University Shenzhen 518060 China National University of Singapore (Suzhou) Research Institute 377 Lin Quan Street Suzhou Industrial Park Suzhou 215123 China

Two-dimensional （2D） layered transition metal dichalcogenides （TMDs） have attracted enormous research interests and efforts towards the development of versatile electronic and optical devices, owing to their extraordinary and unique fundamental properties and remarkable prospects in nanoelectronic applications. Among the TMDs, tungsten diselenide （WSe2） exhibits tunable ambipolar transport characteristics and superior optical properties such as high quantum efficiency. Herein, we demonstrate significant enhancement in the device performance of WSe2 phototransistor by in situ surface functionalization with cesium carbonate （Cs2CO3）. WSe2 was found to be strongly doped with electrons after Cs2CO3 modification. The electron mobility of WSe2 increased by almost one order of magnitude after surface functionalization with 1.6-nm-thick Cs2CO3 decoration. Furthermore, the photocurrent of the WSe2-based phototransistor increased by nearly three orders of magnitude with the deposition of 1.6-nm-thick Cs2CO3. Characterizations by in situ photoelectron spectroscopy techniques confirmed the significant surface charge transfer occurring at the Cs2COB/WSe2 interface. Our findings coupled with the tunable nature of the surface transfer doping method establish WSe2 as a promising candidate for future 2D materials- based optoelectronic devices.

关键词： WSe2 in situ surface transfer doping performance enhancement phototransistor cesium carbonate

金属二聚体和氮共掺杂石墨烯(Gra)M_(2)N_(6)-Gra(M=Cr-Cu)的NO_(2)吸附特性理论研究

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原子与分子物理学报 2025年第5期42卷 35-42页

作者：张展博余娇魏亚茹张轩靳鑫张子音杨保成张雷雷黄河科技学院纳米功能材料研究所郑州450063

NO_(2)是空气污染物的主要成分之一,设计和开发高效的气敏传感器对NO_(2)进行检测具有重要意义.本工作利用基于密度泛函理论(DFT)的第一性原理计算方法对不同过渡金属原子形成的金属二聚体和氮共掺杂石墨烯(Gra)M_(2)N_(6)-Gra(M=Cr-Cu)... 详细信息

NO_(2)是空气污染物的主要成分之一,设计和开发高效的气敏传感器对NO_(2)进行检测具有重要意义.本工作利用基于密度泛函理论(DFT)的第一性原理计算方法对不同过渡金属原子形成的金属二聚体和氮共掺杂石墨烯(Gra)M_(2)N_(6)-Gra(M=Cr-Cu)的NO_(2)吸附特性进行了研究.结果表明,NO_(2)分子与M_(2)N_(6)-Gra之间均存在明显的化学吸附作用.其中,Ni_(2)N_(6)-Gra和Cu_(2)N_(6)-Gra体系具备较为适中的恢复时间(分别约为5秒和14分钟),这意味着这两个体系是开发新型NO_(2)气敏材料的潜在候选者.其它体系(M_(2)N_(6)-Gra,M=Cr-Co)强的吸附作用导致恢复时间过长,从而使得它们不适合作为NO_(2)气敏材料.这一研究不仅有望为设计和开发性能优异的新型NO_(2)气敏材料提供有益理论指导,还将有益于人们深入认识M_(2)N_(6)-Gra材料的NO_(2)电催化合成NO或NH 3性能.

关键词： M_(2)N_(6)-Gra NO_(2)吸附密度泛函理论

Geometry-Aware ICP for Scene Reconstruction from RGB-D Camera

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Journal of Computer Science & Technology 2019年第3期34卷 581-593页

作者： bo Ren Jia-Cheng Wu ya-lei Lv Ming-Ming Cheng Shao-Ping Lu College of Computer Science Nankai UniversityTianjin 300350China

The Iterative Closest Point (ICP) scheme has been widely used for the registration of surfaces and point ***, when working on depth image sequences where there are large geometric planes with small (or even without) details,existing ICP algorithms are prone to tangential drifting and erroneous rotational estimations due to input device *** this paper, we propose a novel ICP algorithm that aims to overcome such drawbacks, and provides significantly stabler registration estimation for simultaneous localization and mapping (SLAM) tasks on RGB-D camera inputs. In our approach,the tangential drifting and the rotational estimation error are reduced by: 1) updating the conventional Euclidean distance term with the local geometry information, and 2) introducing a new camera stabilization term that prevents improper camera movement in the calculation. Our approach is simple, fast, effective, and is readily integratable with previous ICP algorithms. We test our new method with the TUM RGB-D SLAM dataset on state-of-the-art real-time 3D dense reconstruction platforms, i.e., ElasticFusion and Kintinuous. Experiments show that our new strategy outperforms all previous ones on various RGB-D data sequences under different combinations of registration systems and solutions.

关键词： ICP(iterative closest point) RGB-D tangential drifting rotational estimation covariance matrix

Inhibitory effects of genistein on metastasis of human hepatocellular carcinoma

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World Journal of Gastroenterology 2009年第39期15卷 4952-4957页

作者： yan Gu Cheng-Fang Zhu ya-lei Dai Qiang Zhong bo Sun Department of General Surgery Shanghai Ninth Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200011 China Department of Immunology Tongji University School of Medicine Shanghai 200092 China

AIM： To investigate the inhibitory effects of genistein on metastasis of MHCC97-H hepatocellular carcinoma cells and to explore the underlying mechanism. METHODS： MHCC97-H hepatocellular carcinoma cells were exposed to genistein. A cell attachment assay was carried out in a microculture well pre-coated with fibronectin. The invasive activity of tumor cells was assayed in a transwell cell culture chamber, and cell cycle and apoptosis were evaluated by a functional assay. In addition, the expression and phosphorylation of FAK were detected by Western blotting. In situ xenograft transplantation of hepatocellular carcinoma was performed in 12 nude mice and lung metastasis of hepatocellular carcinoma was observed. RESULTS： Genistein significantly inhibited the growth of MHCC97-H cells in vitro. Adhesion and invasiveness of MHCC97-H cells were inhibited in a concentrationdependent fashion, and the inhibitory effect of genistein was more potent in the 10 i^g/mL and 20 i^g/ mL genistein-treated groups. Genistein caused G0/G1 cell cycle arrest, an S phase decrease, and increased apoptosis. The expression and phosphorylation of FAK in MHCC-97H cells were significantly decreased. In situ xenograft transplantation of hepatocellular carcinoma was also significantly suppressed by genistein. The number of pulmonary micrometastatic loci in the genistein group was significantly lower compared with the control group （12.3 ± 1.8 vs 16.6± 2.6, P 〈 0.05）. CONCLUSION： Genistein appears to be a promising agent in the inhibition of metastasis of hepatocellular carcinoma.

关键词： Human hepatocellular carcinoma Genistein Metastasis

The highest melting point material:Searched by Bayesian global optimization with deep potential molecular dynamics

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Journal of Advanced Ceramics 2023年第4期12卷 803-814页

作者： Yinan Wang bo Wen Xingjian Jiao ya Li lei Chen Yujin Wang Fu-Zhi Dai Artificial Intelligence for Science Institute Beijing 100084China Science and Technology on Advanced Functional Composite Laboratory Aerospace Research Institute of Materials&Processing TechnologyBeijing 100076China College of Computer Science and Technology Heilongjiang Institute of TechnologyHarbin 150001China Institute for Advanced Ceramics School of Materials Science and EngineeringHarbin Institute of TechnologyHarbin 150001China Key Laboratory of Advanced Structural-Functional Integration Materials&Green Manufacturing Technology Harbin Institute of TechnologyHarbin 150001China DP Technology Bejing 100080China

The interest in refractory materials is increasing rapidly in recent decades due to the development of hypersonic ***,the substance that has the highest melting point(Tm)keeps a secret,since precise measurements in extreme conditions are overwhelmingly *** the present work,an accurate deep potential(DP)model of a Hf-Ta-C-N system was first trained,and then applied to search for the highest melting point material by molecular dynamics(MD)simulation and Bayesian global optimization(BGO).The predicted melting points agree well with the experiments and confirm that carbon site vacancies can enhance the melting point of rock-saltstructure *** solid solution with N is verified as another new and more effective melting point enhancing approach for HfC,while a conventional routing of the solid solution with Ta(e.g.,HfTa_(4)C_(5))is not suggested to result in a maximum melting *** highest melting point(~4236 K)is achieved with the composition of HfCo.638No.271,which is~80 K higher than the highest value in a Hf-C binary *** mechanism of the N addition is believed to be unstable C-N and N-N bonds in liquid phase,which reduces liquid phase entropy and renders the liquid phase less *** improved melting point and less gas generation during oxidation by the addition of N provide a new routing to modify thermal protection materials for the hypersonic vehicles.

关键词： melting point(T_(m)) carbides carbonitrides deep potential(DP) Bayesiannglobal optimization(BGO)

Compensation Model for Shape Measuring of Cold Strip Rolling

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Journal of Iron and Steel Research International 2010年第6期17卷 21-26页

作者： YU Bing-qiang SUN ya-bo LIU Hong-min YOU lei PENG yan Engineering Research Center of Rolling Equipment and Complete Technology of Ministry of Education Yanshan University Qinhuangdao 066004 Hebei China

Some unavoidable factors in the process of cold strip shape measurement interfere with the shape meter, so the shape measuring results cannot reflect the true shape of the strip and the measuring precision is low. The influ- ences of the measuring error of the strip edges, the transverse temperature difference of the strip, the deflection of shape detection roller, and the shape of the strip coil on the shape measuring results were analyzed in detail, and the corresponding compensation models were established. The simulation calculation and analysis were carried out on a cold strip mill, and a number of disciplinarian cognitions were obtained.

关键词： cold strip rolling shape measuring compensation model