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Field-effect transistor with a chemically synthesized MoS2 sensing channel for label-free and highly sensitive electrical detection of DNA hybridization

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Nano Research 2015年第7期8卷 2340-2350页

作者： Doo-Won Lee Jinhwan Lee II Yung Sohn bo-Yeong Kim Young Min Son Hunyoung Bark JaehyuckJung Minseok choi Tae Hyeong Kim Changgu Lee Nae-Eung Lee Department of Advanced Materials Science & Engineering Sungkyunkwan University Suwon Gyunggi-do 440-746 Republic of Korea Department of Mechanical Engineering Sungkyunkwan University Suwon Gyunggi-do 440-746 Republic of Korea SKKU Advanced Institute of Nanotechnology (SAINT) Sungkyunkwan University Suwon Gyunggi-do 440-746 Republic of Korea Samsung Advanced Institute for Health Sciences & Technology (SAIHST) Sungkyunkwan University Suwon Gyeonggi-do 440-746 Republic of Korea New Materials Team Future Device R&D Department LG Electronics Advanced Research Institute Seou1137-724 Republic of Korea These authors contributed equally to this work.

A field-effect transistor （FET） with two-dimensional （2D） few-layer MoS2 as a sensing-channel material was investigated for label-free electrical detection of the hybridization of deoxyribonucleic acid （DNA） molecules. The high-quality MoS2-channel pattern was selectively formedthrough the chemical reaction of the Mo layer with H2S gas. The MoS2 FET was very stable in an electrolyte and inert to pH changes due to the lack of oxygen-containing functionalities on the MoS2 surface. Hybridization of single-stranded target DNA molecules with single-stranded probe DNA molecules physically adsorbed on the MoS2 channel resulted in a shift of the threshold voltage （Vt,） in the negative direction and an increase in the drain current. The negative shift in Vth is attributed to electrostatic gating effects induced by the detachment of negatively charged probe DNA molecules from the channel surface after hybridization. A detection limit of 10 fM, high sensitivity of 17 mWdec, and high dynamic range of 106 were achieved. The results showed that a bio-FET with an ultrathin 2D MoS2 channel can be used to detect very small concentrations of target DNA molecules specifically hybridized with the probe DNA molecules.

关键词： two-dimensional(2D)materials, MoS2,field-effect transistor, biosensor,deoxyribonucleic acid (DNA) hybridization

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A Pilot Study on the Effect of Pelvic Exercise on Standing Balance in Patients with Incomplete Cervical Spinal

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International Journal of Clinical Medicine 2013年第3期4卷 123-132页

作者： bo-Ra choi Ju-Hyun Kim Junghwan Kim Department of Physical Therapy College of Public Health & Welfare Yongin University Yongin South Korea. Laboratory of Health Science & Nanophysiotherapy Department of Physical Therapy Graduate School Yongin University Yongin South Korea

The purpose of this study was to evaluate the effect of pelvic exercise on standing balance in patients with incomplete cervical spinal cord injury for healthy rehabilitation. 11 patients (8 men and 3 women) with levels of incomplete injury between cervical 3 and cervical 6 who were participated in a study measuring the standing balance using an interactive balance system (IBS) before and after pelvic exercise. The index of standing balance after pelvic exercise was significantly lower than before exercise in patients with incomplete cervical cord injury. The greatest improvements were observed in incomplete cervical spinal cord injury with neurologic levels of cervical 3 and 4 with Brown-Sequard syndrome. These results suggest that the pelvic exercise for rehabilitation significantly improved in part standing balance in patients with incomplete cervical spinal cord injury.

关键词： Incomplete Spinal Cord Injury Standing Balance Pelvic Exercise Rehabilitation

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Construction of recombinant human nerve growth factor beta adenovirus and evaluation of its function An in vitro and in vivo study

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Neural Regeneration Research 2010年第16期5卷 1261-1269页

作者： En-Feng Gao Si-Ho choi Mi-Ae Sung bo-Han Li Samir Jabaiti Sang Bae Yoo Sung-June Kim Soung-Min Kim Jeong Won Jahng Jong-Ho Lee Department of Stomatology China-Japan Friendship Hospital Beijing 100029 China Center for Stem Cell and Regenerative Medicine Keck School of Medicine University of Sourthern Californina USA Department of Oral and Maxillofacial Surgery & Dental Research Institute Seoul National University Seoul Korea Department of Plastic and Reconstructive Surgery Jordan University Hospital Amman Jordan Department of Electrical Engineering School of Electrical and Computer Science Seoul National University Seoul Korea

Exogenous delivery of nerve growth factor （NGF） promotes neural regeneration. However, the short half-life limits delivery efficacy. Therefore, a long-term, efficient, local delivery tool or scheme is needed. The purpose of this study was to construct a functioning, recombinant, adenoviral vector carrying human NGF-β （hNGF-β） DNA, and to measure expression of the constructed vector in vitro and in vivo. rhNGF-β adenoviral vector containing full-length hNGF-β cDNA was generated by homologous recombination in Escherichia CoIL The rhNGF-β adenovirus was packaged and amplified in human embryonic kidney HEK293 cells. Transformation efficiency, expression and function of rhNGF-β adenovirus for primary Schwann cells, Schwann cell lines, human embryonic kidney HEK 293 cells, CRH myoblasts, and NIH3T3 fibroblasts were evaluated. Subsequently, expression of rhNGF-β adenovirus at the peripheral nerve of rat was also assessed. Recombinant adenoviral vector carrying hNGF-β was successfully constructed and confirmed by restriction endonuclease analysis and DNA sequence analysis. Green fluorescent protein expression was observed in 90% of rhNGF-β adenovirus-infected cells （primary Schwann cells, Schwann cell line, human embryonic kidney HEK 293 cells, CRH myoblasts, and NIH3T3 fibroblasts） compared with non-infected cells. Total mRNA isolated from rhNGF-β adenovirus-infected cells exhibited strong expression. Maximum NGF release was induced by primary cultured Schwann cells at 4 days after infection, which steadily continued for 14 days. PC-12 cells exposed to media conditioned with rhNGF-β adenovirus-infected Schwann cells exhibited increased neurite extension. In vivo experiment revealed that the injected rhNGF-β adenovirus was transfected into the cells at the injected site and promoted expression of NGF, p75NTR and brain derived neurotrophic factor at the sciatic nerve and dorsal root ganglia.

关键词： nerve growth factor Schwann cell peripheral nerve regeneration adenoviral vector HEK293 cells myoblasts fibroblasts

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Effect of the secondary air distribution layer on separation density in a dense-phase gas–solid fluidized bed

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International Journal of Mining Science and Technology 2015年第6期25卷 969-973页

作者： Lv bo Luo Zhenfu Zhang bo Zhao Yuemin Zhou Chenyang Yuan Wenchao School of Chemical Engineering and Technology China University of Mining and Technology

Dry coal separation has been the most significant process in the field of coal beneficiation to date, because of its special advantage of operation with no water consumption. Mineral dry separation research has received wide attention, particularly in countries and regions experiencing drought and water shortages. During the process of dense coal gas-solid fluidized bed beneficiation, the material is stratified according to its density; the high density material layer remains at the bed bottom, and thus the high density coarse particle bed becomes an important infuencing factor in fluidized bed stability. In the steady fluidization stage, a small number of large radius bubbles are the direct cause of unsteady fluidization in the tradi- tional fluidized bed. The dispersion effect of the secondary air distribution bed for air flow is mainly apparent in the gas region; when the particle size exceeds 13 mm, the secondary air distribution bed has a synergistic effect on the density stability of the upper fluidized layer. When the particle size is small, especially when less than 6 ram, particles will constantly move, accounting for instability of the secondary air distribution bed and distorting the stability of the upper fluidized bed. Under optimum operation conditions, the probable deviation E of gas-solid separation fluidized with a high density coarse particle layer can be as low as 0.085 g/cm3.

关键词： Secondary air distribution layerDry coal separationGas-solid fluidized bedParticle size

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Weave-pattern-dependent fabric piezoelectric pressure sensors based on polyvinylidene fluoride nanofibers electrospun with 50 nozzles

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npj Flexible Electronics 2022年第1期6卷 699-707页

作者： Da Bin Kim Ju Han Sun Min Sung Min Seong Kim bo Kyoung choi Sung Jun Park Hyae Rim Hong Hong Je choi Byeong Kon Kim Chung Hee Park Jong Hoo Paik Joon-Seok Lee Yong Soo Cho Department of Materials Science and Engineering Yonsei UniversitySeoul 03722Republic of Korea Department of Textile Engineering and Technology Yeungnam UniversityGyeongsan 38541Republic of Korea Deparment of Textile Merchandising and Fashion DesignSeoul National UniversitySeoul 08826Republic of Korea Samsung Electro-Mechanics Co.Ltd. Suwon 16674Republic of Korea Ceratorq Inc. Seoul 08511Republic of Korea Electronic Materials and Component Center Korea Institute of Ceramic Engineering and TechnologyJinju 52851Republic of Korea

Wearable pressure sensors having versatile device structures have been extensively investigated to achieve high sensitivity under mechanical ***,we introduce piezoelectric pressure sensors based on fabrics woven using polyvinylidene fluoride(PVDF)weft and polyethylene terephthalate(PET)warp yarns with different weave structures:1/1(plain),2/2,and 3/3 weft rib *** dependence of the pressure-sensing performance on the weave pattern is demonstrated with an actual large-scale fabric up to the~2 m *** optimized pressure sensor with a 2/2 weft rib pattern produced a high sensitivity of 83 mV N−1,which was 245%higher than that of the 1/1 *** detection performance of the optimal fabric was extensively evaluated with a variety of ambient input sources,such as pressing,bending,twisting,and crumpling,as well as various human ***,a large allfabric pressure sensor with arrayed touch pixel units demonstrated highly sensitive and stable sensing performance.

关键词： pattern fluoride piezoelectric

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基于压缩残差网络的雷达辐射源识别方法研究

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强激光与粒子束 2024年第4期36卷 113-122页

作者：郭恩泽刘正堂崔博刘国彬史航宇蒋旭中国人民解放军63893部队河南洛阳471003 中国人民解放军63896部队河南洛阳471003

针对低信噪比条件下,现有的雷达辐射源信号识别方法存在识别正确率低、时效性差的问题,提出了一种基于压缩残差网络的雷达辐射源信号识别方法。首先,利用choi-Williams分布的时频分析方法将时域信号转换为二维时频图像;然后,根据应用场... 详细信息

针对低信噪比条件下,现有的雷达辐射源信号识别方法存在识别正确率低、时效性差的问题,提出了一种基于压缩残差网络的雷达辐射源信号识别方法。首先,利用choi-Williams分布的时频分析方法将时域信号转换为二维时频图像;然后,根据应用场景特点,选择卷积神经网络(Convolutional Neural Networks,CNN)“压缩”范围;最后,构建压缩残差网络来自动提取图像特征并完成分类。仿真实验结果表明,在同等体量的设计下,与当前较为常用的标准CNN以及ResNet模型相比,所提模型能够降低信号识别运行时间约88%,在信噪比为-14 dB条件下对14种雷达辐射源信号的平均识别率高约5%。提供了一种高效的雷达辐射源信号智能识别方法,具有潜在的工程应用前景。

关键词：压缩残差网络时频分析雷达辐射源识别深度学习扩张卷积

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PIV Measurement of Velocity Distribution in the Moderator Circulation Test (MCT)

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Journal of Power and Energy Engineering 2014年第9期2卷 74-80页

作者： Hyoung Tae Kim bo Wook Rhee Hwa-Lim choi Severe Accident and PHWR Safety Research Division Korea Atomic Energy Research Institute (KAERI) Daejeon Republic of Korea

Korea Atomic Energy Research Institute (KAERI) has been carrying out a scaled-down moderator test program to simulate the CANDU-6 moderator circulation phenomena during steady state operation and accident conditions. The Moderator Circulation Test (MCT) facility was designed and constructed as 1/4 scale of the prototype of CANDU-6 reactor. In the present work spatial distribution of two-dimensional velocity in the MCT facility under isothermal condition was measured using planar Particle Image Velocimetry (PIV) system which consists of double pulsed laser, synchronizer, and high speed camera. TSI’s Insight TM 4G software was used to perform PIV image capturing, PIV calculation and post processing. 10 μm sized silver-coated hollow sphere particles were used as flow tracer particles. Multiple experiments were conducted to cover large area of the MCT facility with limited field of view of a single camera. Instantaneous and averaged velocity field were analyzed for each spatial position and flow rate. This research is capable of offering validation data for self-reliant CFD tools to predict moderator subcooling margin in CANDU-6 reactor.

关键词： PIV CANDU-6 Moderator Circulation Test (MCT) Moderator Subcooling

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Usefulness of magnifying endoscopy for iodine-unstained lesions in a high-risk esophageal cancer population

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World Journal of Gastroenterology 2010年第37期16卷 4709-4715页

作者： Ik Seong choi Jae Young Jang Won Young Cho Tae Hee Lee Hyun Gun Kim bo Young Lee Soung Won Jeong Joo Young Cho Joon Seong Lee So Young Jin Institute for Digestive Research SoonChunHyang University College of Medicine

AIM: To investigate the usefulness of magnified observations of iodine-unstained esophageal lesions in the histological diagnosis of esophageal mucosa abnormalities, in high-risk esophageal cancer groups. METHODS: The subjects included 38 patients who had at least one of the four criteria known to be highrisk factors for esophageal cancer. Following endoscopic observation, magnified observations were performed on iodine-unstained lesions of the esophagus. The total number of lesions was 43. These lesions were classified as type A (clear papilla), type B (fused papilla), and type C (non-visible papilla) according to the findings. Tissue biopsy was then carried out. Finally the histological findings were graded in terms of histological factors, and their relationships were compared. RESULTS: Of the 43 lesions, 11 were type A, 17 were type B, and 15 were type C under magnifying endoscopy. Histological findings such as inflammatory cell infiltration and basal cell hyperplasia were significantly increased in type B and type C lesions compared with type A lesions (P < 0.05). Low-grade esophageal dysplasia was apparent in 1 (9%) of 11 type A lesions, in 3 (18%) of 17 type B lesions, and in 6 (40%) of 15 type C lesions, with the highest rate in type C. CONCLUSION: Magnified observations of the esophagus, classified by papillary aspects using magnifying endoscopy of iodine-unstained lesions in high-risk esophageal cancer groups, are considered useful in estimating dysplasia and inflammation of esophageal mucosa.

关键词： Esophageal cancer Iodine Magnifying endoscopy

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A novel cytokine consisting of the p40 and EBI3 subunits suppresses experimental autoimmune arthritis via reciprocal regulation of Th17 and Treg cells

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Cellular & Molecular Immunology 2022年第1期19卷 79-91页

作者： Seon-Yeong Lee Su-Jin Moon Young-Mee Moon Hyeon-Beom Seo Jun-Geol Ryu A ram Lee Chae Rim Lee Da-Som Kim Yang-Mi Her Jeong Won choi Seung-Ki Kwok Sung-Hwan Park Mi-La Cho The Rheumatism Research Center The Catholic University of KoreaSeoulSouth Korea Division of Rheumatology Department of Internal MedicineSeoul St.Mary’s HospitalCollege of MedicineThe Catholic University of KoreaSeoulSouth Korea Department of Biomedicine&Health Sciences College of MedicineThe Catholic University of KoreaSeoulSouth Korea Department of Medical Lifescience College of MedicineThe Catholic University of KoreaSeoulSouth Korea These author contributed equally:Seon-Yeong Lee Su-Jin MoonYoung-Mee Moon

OBJECTIVE:The interleukin(IL)-12 cytokine family is closely related to the development of T helper cells,which are responsible for autoimmune disease enhancement or ***-12 family members are generally heterodimers and share threeα-subunits(p35,p19,and p28)and twoβ-subunits(p40 and EBI3).However,aβ-sheet p40 homodimer has been shown to exist and antagonize IL-12 and IL-23 signaling ***,we assumed the existence of a p40-EBI3 heterodimer in nature and sought to investigate its role in immune ***:The presence of the p40-EBI3 heterodimer was confirmed by ELISA,immunoprecipitation,and western blotting.A p40-EBI3 vector and p40-EBI3-Fc protein were synthesized to confirm the immunological role of this protein in mice with collageninduced arthritis(CIA).The anti-inflammatory effects of p40-EBI3 were analyzed with regard to clinical,histological,and immune cell-regulating features in mice with ***:Clinical arthritis scores and the expression levels of proinflammatory cytokines(e.g.,IL-17,IL-1β,IL-6,and TNF-α)were significantly attenuated in p40-EBI3-overexpressing and p40-EBI3-Fc-treated mice with CIA compared to vehicle-treated mice with *** joint damage and vessel formation-related gene expression were also reduced by p40-EBI3 heterodimer *** vitro,the p40-EBI3-Fc protein significantly suppressed the differentiation of Th17 cells and reciprocally induced CD4+CD25+Foxp3+(regulatory T)cells.p40-EBI3 also inhibited osteoclast formation in a concentrationdependent ***:In this study,p40-EBI3 ameliorated proinflammatory conditions both in vivo and in *** propose that p40-EBI3 is a novel anti-inflammatory cytokine involved in suppressing the immune response through the expansion of Treg cells and suppression of Th17 cells and osteoclastogenesis.

关键词： cytokine p40-EBI3 Rheumatoid arthritis Autoimmune disease regulatory T cell

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Gut microbiota as a potential key to modulating humoral immunogenicity of new platform COVID-19 vaccines

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Signal Transduction and Targeted Therapy 2023年第6期8卷 2447-2450页

作者： Hye Seong bo Kyu choi Young-Hee Han Jun Hyoung Kim Jeong-An Gim Sooyeon Lim Ji Yun Noh Hee Jin Cheong Woo Joo Kim Joon Young Song Department of Internal Medicine Korea University College of MedicineSeoulRepublic of Korea Asia Pacific Influenza Institute Korea University College of MedicineSeoulRepublic of Korea Vaccine Innovation Center Korea University College of MedicineSeoulRepublic of Korea Department of Biomedical Systems Informatics Yonsei University College of MedicineSeoulRepublic of Korea Department of Food and Nutrition Chungbuk National UniversityCheongjuRepublic of Korea Department of Internal Medicine Chungbuk National University HospitalCheongjuRepublic of Korea Medical Science Research Center Korea University College of MedicineSeoulRepublic of Korea

Dear Editor,Coronavirus disease 2019(COVID-19)is a novel respiratory infectious disease,caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),which led to a global *** vaccination is the best measure to overcome a pandemic,the immunogenicity of vaccines can be influenced by diverse factors,including intrinsic(age,sex,genetics,and comorbidities),extrinsic(diet,nutrition,and behavior),and vaccine-associated characteristics.

关键词： vaccines respiratory acute

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