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The RNA-binding domain of DCL3.is required for long-distance RNAi signaling

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aBIOTECH 2024年第1期5卷 17-28页

作者： Jie Li bo-Sen Zhang Hua-Wei Wu Cheng-Lan Liu Hui-Shan Guo Jian-Hua zhao State Key Laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China CAS Center for Excellence in Biotic Interactions University of the Chinese Academy of SciencesBeijing 100049China Qilu Zhongke Academy of Modern Microbiology Technology Jinan 250022China

Small RNA(sRNA)-mediated RNA silencing(also known as RNA interference,or RNAi)is a conserved mechanism in eukaryotes that includes RNA degradation,DNA methylation,heterochromatin formation and protein translation *** plants,sRNAs can move either cell-to-cell or systemically,thereby acting as mobile silencing signals to trigger noncell autonomous ***,whether and what proteins are also involved in noncell autonomous silencing have not been *** this study,we utilized a previously reported inducible RNAi plant,PDSi,which can induce systemic silencing of the endogenous PDS gene,and we demonstrated that DCL3.is involved in systemic PDS silencing through its RNA binding *** confirmed that the C-terminus of DCL3.including the predicted RNA-binding domain,is capable of binding short *** affecting RNA binding,but not processing activity,reduced systemic PDS silencing,indicating that DCL3.binding to RNAs is required for the induction of systemic *** mosaic virus infection assays showed that the RNA-binding activity of DCL3.is required for antiviral RNAi in systemically noninoculated *** findings demonstrate that DCL3.acts as a signaling agent involved in noncell autonomous silencing and an antiviral effect in addition to its previously known function in the generation of 24-nucleotide sRNAs.

关键词： RNAi signaling DCL3 RBD RNAs Cucumber mosaic virus

Sorafenib sensitization in tumor therapy by iron overload and AMPK activation

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Nano Research 2024年第7期17卷 6386-6399页

作者： Jun Zhu Shoujie zhao Yejing Zhu Shouzheng Ma Tenghui Han Fenghua Xu Huichen Li bo Wang Zhixiong Guo Da Chen Yongquan Qu Zhimin Tian Junlong zhao Lei Liu State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers National Clinical Research Center for Digestive DiseasesXijing HospitalFourth Military Medical UniversityXi’an 710032China Department of General Surgery The Southern Theater Air Force HospitalGuangzhou 510602China Department of General Surgery Tangdu HospitalFourth Military Medical UniversityXi’an 710038China Department of Thoracic Surgery Tangdu HospitalFourth Military Medical UniversityXi’an 710038China Department of Neurology Airborne Army HospitalWuhan 430012China Key Laboratory of Modern Teaching Technology(Ministry of Education) Shaanxi Normal UniversityXi’an 710062China State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers Department of Biochemistry and Molecular BiologySchool of Basic MedicineFourth Military Medical UniversityXi’an 710032China Key Laboratory of Special Functional and Smart Polymer Materials of Ministry of Industry and Information Technology School of Chemistry and Chemical EngineeringNorthwestern Polytechnical UniversityXi’an 710072China State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers Department of Medical Genetics and Developmental BiologyFourth Military Medical UniversityXi’an 710032China Department of Pediatrics Tangdu HospitalFourth Military Medical UniversityXi’an 710000China

Sorafenib,as a first-line drug for advanced hepatocellular carcinoma(HCC),could trigger ferroptosis by inhibiting cystine/glutamate ***,low-level intracellular iron and insufficient activation of adenosine monophosphate(AMP)-activated protein kinase(AMPK)confer impaired response to *** this study,a unique sorafenib nanocomposite dexterously modified with Fe-Material of Institut Lavoisier(sora@Fe-MIL)was synthesized to escalate intracellular iron level and activate AMPK,further potentiating the ferroptotic effect of ***,this strategic deployment of sora@Fe-MIL triggered an extensive demise of cancer cells,while manifesting negligible deleterious impact on normal *** prominent ferroptosis biomarkers,glutathione peroxidase 4(GPX4)and solute carrier family 7 member 11(SLC7A11),underwent pronounced downregulation,underscoring the efficacy of this strategy in inducing ***,the bioactivity of AMPK was considerably elevated,and its downstream targets were conspicuously inhibited by the treatment with sora@*** orthotopic HCC animal models,we observed a substantial suppression of primary in situ tumor growth,and ribonucleic acid(RNA)sequencing elucidated an elevated degree of ferroptosis and AMPK activation with the treatment of sora@*** conclusion,we proposed that the meticulously designed strategy for secure and efficacious iron release and AMPK activation could significantly potentiate the ferroptotic impact of sorafenib,thus resuscitating its therapeutic response in HCC patients.

关键词： anti-tumor ferroptosis Fe-Material of Institut Lavoisier(Fe-MIL) sorafenib adenosine monophosphate(AMP)-activated protein kinase(AMPK)

Distribution and Formation Causes of PM_(2.5) and O_(3. Double High Pollution Events in China during 2013.20

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Advances in Atmospheric Sciences 2024年第6期41卷 1235-1250,I0004-I0021页

作者： Zhixuan TONG Yingying YAN Shaofei KONG Jintai LIN Nan CHEN bo ZHU Jing MA Tianliang zhao Shihua QI Department of Atmospheric Science School of Environmental SciencesChina University of GeosciencesWuhan 430074China State Key Laboratory of Atmospheric boundary Layer Physics and Atmospheric Chemistry Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China Research Centre for Complex Air Pollution of Hubei Province Wuhan 430074China Laboratory for Climate and Ocean–Atmosphere Studies Department of Atmospheric and Oceanic SciencesSchool of PhysicsPeking UniversityBeijing 100871China Eco-Environmental Monitoring Centre of Hubei Province Wuhan 430074China Key Laboratory of Atmospheric Chemistry China Meteorological AdministrationBeijing 100081China School of Atmospheric Physics Nanjing University of Information Science and TechnologyNanjing 210044China State Key Laboratory of Biogeology and Environmental Geology China University of GeosciencesWuhan 430074China

Fine particulate matter(PM_(2.5))and ozone(O_(3.)double high pollution(DHP)events have occurred frequently over China in recent years,but their causes are not completely *** this study,the spatiotemporal distribution of DHP events in China during 2013.20 is *** synoptic types affecting DHP events are identified with the Lamb–Jenkinson circulation classification *** meteorological and chemical causes of DHP events controlled by the main synoptic types are further *** show that DHP events(1655 in total for China during 2013.20)mainly occur over the North China Plain,Yangtze River Delta,Pearl River Delta,Sichuan Basin,and Central *** occurrence frequency increases by 5.1%during 2013.15,and then decreases by 56.1%during 2015–*** main circulation types of DHP events are“cyclone”and“anticyclone”,accounting for over 40%of all DHP events over five main polluted regions in China,followed by southerly or easterly flat airflow types,like“southeast”,“southwest”,and“east”.Compared with non-DHP events,DHP events are characterized by static or weak wind,high temperature(20.9℃ versus 23.1℃)and low humidity(70.0%versus 64.9%).The diurnal cycles of meteorological conditions cause PM_(2.5)(03.0–1200 LST,Local Standard Time=UTC+8 hours)and O_(3.(1500–2100 LST)to exceed the national standards at different periods of the DHP *** pollutant conversion indices further indicate the rapid secondary conversions during DHP events,and thus the concentrations of NO_(2),SO_(2) and volatile organic compounds decrease by 13.1%,4.7%and 4.4%,*** results of this study can be informative for future decisions on the management of DHP events.

关键词： double high pollution events PM_(2.5) ozone spatiotemporal distribution meteorological causes chemical composition characteristics

Multiple Matching Attenuation Based on Curvelet Domain Extended Filtering

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Journal of Ocean University of China 2024年第4期23卷 924-932页

作者： HUA Qingfeng CHEN Zhang HE Huili TAN Jun CHEN Haifeng LI Guanbao SONG Peng zhao bo JIANG Xiuping Key Laboratory of Marine Geology and Metallogeny First Institute of OceanographyMinistry of Natural ResourcesQingdao 266061China Laboratory for Marine Mineral Resources Qingdao Marine Science and Technology CenterQingdao 266237China College of Marine Geoscience Ocean University of ChinaQingdao 266100China BGP Inc. China National Petroleum CorporationZhuozhou 072750China Key Laboratory of Submarine Geosciences and Prospecting Techniques Ministry of EducationQingdao 266100China

The paper develops a multiple matching attenuation method based on extended filtering in the curvelet domain,which combines the traditional Wiener filtering method with the matching attenuation method in curvelet ***,the method uses the predicted multiple data to generate the Hilbert transform records,time derivative records and time derivative records of Hilbert ***,the above records are transformed into the curvelet domain and multiple matching attenuation based on least squares extended filtering is ***,the attenuation results are transformed back into the time-space *** on the model data and field data show that the method proposed in the paper effectively suppress the multiples while preserving the primaries ***,it has higher accuracy in eliminating multiple reflections,which is more suitable for the multiple attenuation tasks in the areas with complex structures compared to the time-space domain extended filtering method and the conventional curvelet transform method.

关键词： multiple matching attenuation curvelet domain extended filtering

Genome-wide DNA methylome analysis reveals a critical role of methylation-dysregulated lncRNAs in autophagy regulation in glioblastoma

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Genes & Diseases 2024年第5期11卷 51-54页

作者： Hongying zhao Ying Liu Meiting Fei Lin bo Lixia Wang Yaopeng Shu Peiqi Ben Li Wang College of Bioinformatics Science and Technology Harbin Medical UniversityHarbinHeilongjiang 150081China

Glioblastoma(GBM)is the most common primary malignant intracranial tumor with a very poor *** this study,we systematically analyzed the DNA methylation alterations of autophagy-related lncRNAs and their association with drug therapies in *** identified 9 DNA methylation-dysregulated lncRNA regulators of autophagy-related genes as autophagy-related lncRNAs.A dysregulated regulatory network consisting of 9 autophagy-related lncRNAs and 23. differentially expressed autophagy-related genes was *** of small molecule drug candidates that may affect DNA methylation dysregulated lncRNA activity,including 45 drug lncRNA pairs,involving 7 DNA methylation dysregulated lncRNAs and 43.***,we identified a DNA methylation-dysregulated autophagy-related lncRNA MIR155HG as an independent prognostic indicator for GBM.

关键词： regulated alterations drugs

The action mechanism by which C1q/tumor necrosis factor-related protein-6 alleviates cerebral ischemia/reperfusion injury in diabetic mice

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Neural Regeneration Research 2024年第9期19卷 2019-2026页

作者： bo zhao Mei Li Bingyu Li Yanan Li Qianni Shen Jiabao Hou Yang Wu Lijuan Gu Wenwei Gao Department of Anesthesiology Renmin Hospital of Wuhan UniversityWuhanHubei ProvinceChina Central Laboratory Renmin Hospital of Wuhan UniversityWuhanHubei ProvinceChina Department of Critical Care Medicine Renmin Hospital of Wuhan UniversityWuhanHubei ProvinceChina

Studies have shown that C1q/tumor necrosis factor-related protein-6 (CTRP6) can alleviate renal ischemia/reperfusion injury in mice. However, its role in the brain remains poorly understood. To investigate the role of CTRP6 in cerebral ischemia/reperfusion injury associated with diabetes mellitus, a diabetes mellitus mouse model of cerebral ischemia/reperfusion injury was established by occlusion of the middle cerebral artery. To overexpress CTRP6 in the brain, an adeno-associated virus carrying CTRP6 was injected into the lateral ventricle. The result was that oxygen injury and inflammation in brain tissue were clearly attenuated, and the number of neurons was greatly reduced. In vitro experiments showed that CTRP6 knockout exacerbated oxidative damage, inflammatory reaction, and apoptosis in cerebral cortical neurons in high glucose hypoxia-simulated diabetic cerebral ischemia/reperfusion injury. CTRP6 overexpression enhanced the sirtuin-1 signaling pathway in diabetic brains after ischemia/reperfusion injury. To investigate the mechanism underlying these effects, we examined mice with depletion of brain tissue-specific sirtuin-1. CTRP6-like protection was achieved by activating the sirtuin-1 signaling pathway. Taken together, these results indicate that CTRP6 likely attenuates cerebral ischemia/reperfusion injury through activation of the sirtuin-1 signaling pathway.

关键词： brain C1q/tumor necrosis factor-related protein-6 cerebral apoptosis diabetes inflammation ischemia/reperfusion injury neuron neuroprotection oxidative damage Sirt1

Self-consistent and precise measurement of time-dependent radiative albedo of gold based on specially symmetrical triple-cavity Hohlraum

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Matter and Radiation at Extremes 2024年第3期9卷 57-64页

作者： Zhiyu Zhang Yang zhao Xiaoying Han Liling Li bo Qing Lifei Hou Yulong Li YuXue Zhang Huan Zhang Xiangming Liu bo Deng Gang Xiong Min Lv Tuo Zhu Chengwu Huang Tianming Song Yan zhao Yingjie Li Lu Zhang Xufei Xie Jiyan Zhang Jiamin Yang Laser Fusion Research Center China Academy of Engineering PhysicsMianyang 621900China National Key Laboratory of Plasma Physcis Mianyang 621900China Institute of Applied Physics and Computational Mathematics Beijing 100088China

A self-consistent and precise method to determine the time-dependent radiative albedo,i.e.,the ratio of the reemission flux to the incident flux,for an indirect-drive inertial confinement fusion Hohlraum wall material is proposed.A specially designed symmetrical triple-cavity gold Hohlraum is used to create approximately constant and near-equilibrium uniform radiation with a peak temperature of 160 *** incident flux at the secondary cavity waist is obtained from flux balance analysis and from the shock velocity of a standard *** results agree well owing to the symmetrical radiation in the secondary cavity.A self-consistent and precise time-dependent radiative albedo is deduced from the reliable reemission flux and the incident flux,and the result from the shock velocity is found to have a smaller uncertainty than that from the multi-angle flux balance analysis,and also to agree well with the result of a simulation using the HYADES opacity.

关键词： precise cavity radiative

Proteomic study of vitreous in proliferative diabetic retinopathy patients after treatment with aflibercept:a quantitative analysis based on 4D label-free technique

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International Journal of Ophthalmology(English edition) 2024年第4期17卷 676-685页

作者： Ting-Ting Feng Xiang Gao An-Ran Liang bo-Wen zhao Guang-Hui He Song Chen Clinical College of Ophthalmology Tianjin Medical UniversityTianjin 300070China Tianjin Key Laboratory of Ophthalmology and Visual Science Tianjin Eye InstituteTianjin Eye HospitalTianjin 300020China Key Laboratory of Ophthalmology Anyang Eye HospitalAnyang 455000Henan ProvinceChina Department of Ophthalmology Jining No.1 People’s HospitalJining 272011Shandong ProvinceChina

AIM:To identify different metabolites,proteins and related pathways to elucidate the causes of proliferative diabetic retinopathy(PDR)and resistance to anti-vascular endothelial growth factor(VEGF)drugs,and to provide biomarkers for the diagnosis and treatment of ***:Vitreous specimens from patients with diabetic retinopathy were collected and analyzed by Liquid Chromatography-Mass Spectrometry(LC-MS/MS)analyses based on 4D label-free *** differentially expressed proteins(DEPs),Gene Ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway representation and protein interactions were ***:A total of 12 samples were *** proteomics results showed that a total of 58 proteins were identified as DEPs,of which 47 proteins were up-regulated and 11 proteins were *** found that C1q and tumor necrosis factor related protein 5(C1QTNF5),Clusterin(CLU),tissue inhibitor of metal protease 1(TIMP1)and signal regulatory protein alpha(SIRPα)can all be specifically regulated after aflibercept *** functional analysis showed that some DEPs are related to changes in inflammatory regulatory pathways caused by *** addition,protein-protein interaction(PPI)network evaluation revealed that TIMP1 plays a central role in neural *** addition,CD47/SIRPαmay become a key target to resolve anti-VEGF drug resistance in ***:Proteomic analysis is an approach of choice to explore the molecular mechanisms of *** data show that multiple proteins are differentially changed in PDR patients after intravitreal injection of aflibercept,among which C1QTNF5,CLU,TIMP1 and SIRPαmay become targets for future treatment of PDR and resolution of anti-VEGF resistance.

关键词： vitreous proliferative diabetic retinopathy proteome 4D label-free

AGTR1 A1166C gene polymorphism is associated with the effectiveness of valsartan monotherapy in Chinese patients with essential hypertension:A retrospective analysis

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Asian Pacific Journal of Tropical Medicine 2024年第9期17卷 418-424页

作者： Hanzhong Yu Lei Li Shuyao Wei Qianqian Kong Wei Nu bo Dong Yuewu zhao Li Wang Department of Clinical Laboratory the Affiliated Xuzhou Municipal Hospital of Xuzhou Medical UniversityXuzhouJiangsu 221116China Department of Clinical Laboratory Xuzhou Tumor HospitalXuzhouJiangsu 221005China Department of Cardiovascular Medicine the Affiliated Xuzhou Municipal Hospital of Xuzhou Medical UniversityXuzhouJiangsu 221116China

Objective:To investigate whether angiotensinⅡtype 1 receptor(AGTR1 A1166C)gene polymorphism was associated with the effectiveness of valsartan monotherapy in Chinese patients with essential ***:This retrospective analysis included 198 patients(≥18 years of age)who received valsartan monotherapy(80 mg/day)for newly developed essential hypertension at the authors’center between January 1,2020 and December 3.,*** for AGTR1 A1166C gene polymorphism was done by polymerase chain reaction(PCR)-melting curve analysis of genomic DNA from peripheral blood samples.A dominant genetic model for AGTR1 A1166C(AA genotype versus AC+CC genotype)was *** regression analysis of baseline variables and AGTR1 polymorphism was conducted to identify predictors of target blood pressure attainment(3.7±13.5)years,and 58%were *** assays showed that 164 patients had the AA genotype,and 3. patients were of the AC/CC genotype,including 3. with the AC genotype and 4 with the CC *** distribution was consistent with Hardy Weinberg equilibrium.109 Patients(55.1%)attained the blood pressure *** analysis showed that smoking(versus no smoking,HR 0.3.4,95%CI 0.159-0.619,P=0.001)and AGTR1 A1166C AA genotype(versus AC/CC,HR 2.927,95%CI 1.296-6.611,P=0.023.were significant and independent predictors of target attainment.25 Patients(73.5%)with AGTR1 A1166C AC/CC genotype attained the target versus 51.2%(51/164)of patients with AGTR1 A1166C AA genotype(P=0.017).Patients with AGTR1 A1166C AC/CC genotype had a significantly greater reduction in systolic blood pressure[(3..1±10.8)mmHg versus(29.2±11.7)mmHg in AA carriers;(P=0.029)].Conclusions:Hypertensive patients carrying one or two C alleles of the AGTR1 A1166C gene were more responsive to valsartan treatment.

关键词： Essential hypertension AngiotensinⅡtype 1 receptor antagonist Valsartan AGTR1 A1166C Gene polymorphism