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Display of aggregation-prone ligand binding domain of human PPAR gamma on surface of bacteriophage lambda

Acta Pharmacologica Sinica 2006年第1期27卷 91-99页

作者： bo kong~(2,4) wei-{3. ma~(2,3.5)2.institute of health sciences.Shanghai institutes for biological sciences.chinese Academy of sciences.and {13. school of Shanghai jiao tong university.Shanghai 2.002..china 3.Shaughai Research Center of Biotechnology,Shanghai institutes for biological sciences.Chiese Academy og sciences.Shanghai 20023..{23. 4 Graduate school of the chinese Academy of sciences institute of health {2. Shanghai Institutes for Biological Sciences Chinese Academy of Sciences and Medical School of Shanghai Jiao Tong University Shanghai China Graduate school of the chinese Academy of {2. China Shanghai Research Center of Biotechnology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China

Aim:To display the aggregation-prone ligand binding domain (LBD) of the hu-man peroxisome proliferator-activated receptor gamma (PPARγ) on the surface ofbacteriophages to establish an easy screening assay for the identification of PPARγ***:Plasmids were constructed for the expression of the PPARγLBD as a fusion to the N-terminus of the g3. protein of filamentous phage or theC-terminus of the capsid protein D (pD) of phage *** fusion proteinswere expressed in E coli and solubility characteristics were *** against the LBD as well as the pD protein were prepared for Westernblot analysis and phage capture ***:The pD-LBD fusion protein waspartially soluble,whereas the LBD-g3. fusion protein was detected only in theinsoluble *** pD-LBD fusion protein was efficiently incorporated inphage ***,the LBD was shown to be displayed on the surfaceof bacteriophage *** average,the pD-LBD fusion protein accounted for{3.% of the total pD protein in the lambda head ***:The hydro-phobic PPARγ LBD was expressed as a soluble form of fusion protein in E coli anddisplayed on the surface of bacteriophage lambda when it was fused to the lambdapD *** lambda pD fusion system could be used for improving the solu-bility of proteins that tend to form inclusion bodies when expressed in E *** phage particles displaying the LBD of PPARy may be of great value for theidentification of novel PPARγ ligands.

关键词： protein expression lambda phage surface display nuclear receptor peroxisome proliferator-activated receptors ligand binding domain

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Erythropoietin Receptor Positive Circulating Progenitor Cells and Endothelial Progenitor Cells in Patients with Different Stages of Diabetic Retinopathy

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chinese medical {2.Journal 2011年第2期26卷 69-76页

作者： Liu-mei Hu Xia Lei bo ma Yu Zhang Yan Yan Ya-lan Wu Ge-zhi Xu Wen Ye Ling Wang Guo-xu Xu Guo-{2. Xu {3.-ye Li Department of Ophthalmology Peking Union Medical College Hospital Chinese Academy of Medical Sciences ＆ Peking Union Medical College Beijing 100730 China tongji Eye institute and Department of Regenerative Medicine Tongji University School of Medicine Shanghai 200092 China Laboratory of Clinical Visual sciences Institute of Health Sciences Shanghai Institutes for Biological Sciences Chinese Academy of Sciences ＆ Shanghai Jiao Tong University School of Medicine Shanghai 200025 China Department of Ophthalmology Eye and ENT Hospital Fudan University Shanghai 200031 China Department of Ophthalmology Huashan Hospital Affiliated to Fudan University Shanghai 200040 China Department of Ophthalmology Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai 200025 China Department of Ophthalmology Second Affiliated Hospital of Soochow University Suzhou Jiangsu 215004 China Department of Ophthalmology Drexel University College of Medicine Philadelphia PA 19107 USA

Objective To investigate the possible involvement of erythropoietin （EPO）/erythropoietin receptor （EPOR） system in neovascularization and vascular regeneration in diabetic retinopathy （DR）. Methods EPOR positive circulating progenitor cells （CPCs： CD3.^＋） and endothelial progenitor cells （EPCs： CD3.^＋KDR^＋） were assessed by flow cytometry in type 2.diabetic patients with different stages of DR. The cohort consisted of age- and sex-matched control patients without diabetes （n=7）, non-prolif- erative DR （NPDR, n=7）, proliferative DR （PDR, n=8）, and PDR complicated with diabetic nephropathy （PDR-DN, n=7）. Results The numbers of EPOR^＋ CPCs and EPOR^＋ EPCs were reduced remarkably in NPDR corn pared with the control group （{2.th P（0.01）, whereas re{2.unded in PDR and PDR-DN groups in varying degrees. Similar changes were observed in respect of the proportion of EPOR^＋ CPCs in CPCs （NPDR vs. control, P（0.01） and that of EPOR^＋ EPCs in EPCs （NPDR vs. control, P〈0.05）. Conclusion Exogenous EPO, mediated via the EPO/EPOR system of EPCs, may alleviate the impaired vascular regeneration in NPDR, whereas it might aggravate retinal neovascularization in PDR due to a re{2.und of EPOR^＋ EPCs associated with ischemia.

关键词： circulating progenitor cells endothelial progenitor cells erythropoietin re-ceptor diabetic retinopathy

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Synthetic anti-angiogenic genomic therapeutics for treatment of neovascular age-related macular degeneration

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Asian Journal of Pharmaceutical sciences 2021年第5期16卷 623-632页

作者： Jing Wang Xiang Shi Qiyu bo Hong Wang Fang wei {2. Liu Hao Wang Liuwei Zhang Yan Qi Zhen Li Qixian Chen Xiaodong Sun Department of Ophthalmology Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China Shanghai Key Laboratory of Ocular Fundus Diseases Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China Shanghai Engineering Center for Visual Science and Photomedicine Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China National Clinical Research Center for Eye Diseases Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China Ningbo Hygeia medical Technology Co. Ltd.Ningbo 315201China school of Bioengineering Dalian University of TechnologyDalian 116024China College of Pharmacy Dalian Medical UniversityDalian 116044China

In light of the intriguing potential of anti-angiogenic approach in suppressing choroidal neovascularization, we attempted to elaborate synthetic gene delivery systems encapsulating anti-angiogenic plasmid DNA as alternatives of clinical antibody-based therapeutics. Herein, block copolymer of cyclic Arg-Gly-Asp-poly(ethylene glycol)-poly(lysine-thiol) [RGD-PEG-PLys(thiol)] with multifunctional components was tailored in {2.nufacture of core-shell DNA delivery nanoparticulates. Note that the polycationic PLys segments were electrostatically complexed with anionic plasmid DNA into nanoscaled core, and the tethered biocompatible PEG segments presented as the spatial shell(minimizing non-specific reactions in {3. milieu). Furthermore, the aforementioned self-assembly was introduced with redox-responsive disulfide crosslinking due to the thiol coupling. Hence, reversible stabilities, namely stable in extracellular milieu but susceptible to disassemble for liberation of the DNA payloads in intracellular reducing microenvironment, were verified to facilitate transcellular gene transportation. In addition, RGD was installed onto the surface of the proposed self-assemblies with aim of targeted accumulation and internalization into angiogenic endothelial cells given that RGD receptors were specifically overexpressed on their cytomembrane surface. The proposed anti-angiogenic DNA therapeutics were validated to exert efficient expression of anti-angiogenic proteins in endothelial cells and elicit potent inhibition of ocular neovasculature post intravitreous administration. Hence, the present study approved the potential of gene therapy in treatment of choroidal neovascularization. In light of sustainable gene expression properties of DNA therapeutics, our proposed synthetic gene delivery system inspired prosperous potentials in long-term treatment of choroidal neovascularization, which should be emphasized to develop further towards clinical translations.

关键词： Age-related macular degeneration Anti-angiogenesis Gene therapy Polymer Vascular endothelial growth factor

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Induction of a 55 kDa acetylcholinesterase protein during apoptosis and its negative regulation by the Akt pathway

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分子细胞生物学报:英文版 2011年第4期3卷 250-259页

作者： Jing Xie Hua Jiang Yi-Han Wan Ai-Ying Du Kai-Jie Guo Te Liu wei-Yuan Ye Xin Niu jun Wu Xiao-Qin Dong Xue-jun Zhang Laboratory of Molecular Cell Biology Institute of Biochemistry and Cell BiologyShanghai Institutes for Biological SciencesChinese Academy of Sciences320 YueYang RoadShanghai 200031China International Peace maternity and Child Care Center Medical School of Jiao-Tong UniversityShanghai 200030China

Acetylcholinesterase(AChE)is emerging as an important contributor to apoptosis in various cell ***,overexpression of AChE does not initiate apoptosis,and cells which express AChE at basal levels grow normally,suggesting that AChE may function differently between normal and apoptotic *** this study,we determined that an AChE-derived protein(∼55 kDa)positively correlated with cellular apoptotic *** 55 kDa AChE protein was not a result of a novel splice variant of the AChE primary ***,it was determined to be a cleaved fragment of the full-length 68 kDa AChE protein that could not be inhibited by cycloheximide(CHX)but could be suppressed by caspase inhibitors in apoptotic PC-12.***,activation of the Akt cascade abolished the 55 kDa protein,and both AChE protein forms(68 and 55 kDa)accumulated in the nucleus during *** a mouse model for ischemia/reperfusion(I/R)-induced acute renal failure,the 55 kDa AChE protein was detected in the impaired organs but not in the normal ones,and its levels correlated with the genotype of the *** summary,a 55 kDa AChE protein resulting from the cleavage of 68 kDa AChE is induced during apoptosis,and it is negatively regulated by the Akt *** study suggests that an alternative form of AChE may play a role in apoptosis.

关键词： acetylcholinesterase apoptosis 55 kDa AChE caspase PI3./Akt pathway

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Systematic evaluation of IgG responses to SARS-CoV-2.spike protein-derived peptides for monitoring COVID-19 patients

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Cellular & Molecular Immunology 2021年第3期18卷 621-631页

作者： Yang Li Dan-yun La Qing Lei Zhao-wei Xu Feng Wang Hongyan Hou Lingyun Chen jiaoxiang Wu Yan Ren Ming-liang {2. {3. Zhang Hong Chen Caizheng Yu jun-biao Xue Yun-xiao Zheng Xue-ning Wang He-wei Jiang Hai-nan Zhang Huan Qi Shu-juan Guo Yandi Zhang Xiaosong Lin Zongjie Yao Pengfei Pang Dawei Shi wei Wang Xiao Yang Jie Zhou Huiming Sheng Ziyong Sun Hong Shan Xionglin Fan Sheng-ce Tao Shanghai Center for Systems Biomedicine Key Laboratory of Systems Biomedicine(Ministry of Education)Shanghai Jiao Tong UniversityShanghaiChina Department of Pathogen Biology School of Basic MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina Key Laboratory of Gastrointestinal Cancer(Fujian medical {2. Ministry of EducationSchool of Basic Medical SciencesFujian Medical UniversityFuzhouChina Department of Clinical Laboratory Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina SBGI Education Center University of Chinese Academy of SciencesShenzhen 518083China tongren Hospital Shanghai Jiao Tong University School of MedicineShanghaiChina BGI-Shenzhenf Shenzhen 518083 China Department of Public health Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina Center for Interventional Medicine The Fifth Affiliated Hospital of Sun Yat-sen UniversityZhuhai 519000China National institutes for Food and Drug Control Beijing 100050China Foshan Fourth Peopled Hospital Foshan 528000China Key Laboratory of RNA Biology Institute of BiophysicsChinese Academy of SciencesBeijing 100101China

Serological tests play an essential role in monitoring and combating the COVID-19 *** spike protein(S protein),especially the S1 protein,is one of the major reagents used for serological ***,the high cost of S protein production and possible cross-reactivity with other human coronaviruses pose unavoidable *** taking advantage of a peptide microarray with full spike protein coverage,we analyzed 2.43. sera from 858 COVID-19 patients,63.asymptomatic patients and 610 controls collected from multiple clinical *** on the results,we identified several S protein-derived 12.mer peptides that have high diagnostic *** particular,for monitoring the IgG response,one peptide(aa 1148-1159 or S2.78)exhibited a sensitivity(95.5%,95%CI 93.7-96.9%)and specificity(96.7%,95%CI 94.8-98.0%)comparable to those of the S1 protein for the detection of both symptomatic and asymptomatic COVID-19 ***,the diagnostic performance of the S2.78(aa 1148-1159)IgG was successfully validated by ELISA in an independent sample cohort.A panel of four peptides,S1-93.aa 553.564),S1-97(aa 577-588),S1-101(aa 601-612.and S1-105(aa 62.-63.),that likely will avoid potential cross-reactivity with sera from patients infected by other coronaviruses was *** peptides identified in this study may be applied independently or in combination with the S1 protein for accurate,affordable,and accessible COVID-19 diagnosis.

关键词： SARS-CoV-2 Spike peptide Antibody Diagnosis

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Questions about NgAgo

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Protein & Cell 2016年第12期7卷 913-915页

作者： Shawn Burgess Linzhao Cheng Feng Gu Zhiwei Huang Shuo Lin Jinsong Li wei Li wei Qin Yujie Sun Zhou Songyang Wensheng wei Qiang Wu Haoyi Wang Xiaoqun Wang Jing-wei Xiong Jianzhong Xi Hui Yang Bin Zhou bo Zhang junjiu Huang National Human Genome Research institute NIH Bethesda MD 20892 USA Division of Hematology in Department of Medicine Johns HopkinsUniversity School of Medicine Baltimore MD 21205 USA Stem Cell Program in the institute for Cell Engineering Johns Hopkins University School of Medicine Baltimore MD 21205 USA Center for Vision Research Eye Hospital Wenzhou Medical University Wenzhou 325035 China Stem Cell and Functional Genomics Laboratory School of LifeSciences Sun Yat-sen University Guangzhou 510275 China HIT Center of Life sciences Harbin Institute of Technology Harbin 150080 China Department of Molecular Cell and Developmental Biology University of California Los Angeles Los Angeles CA 90095 USA institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China State Key Laboratory of Stem cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing 100101 China College of Chemical Biology and Biotechnology Peking Univer- sity Shenzhen Graduate School Shenzhen 518055 China PKU BIOPIC Beijing 100871 China BIOPIC ICG CLS and School of Life Sciences Peking Univer-sity Beijing 100871 China Center for Comparative Biomedicine Institute of SystemsBiomedicine Shanghai Jiao Tong University Shanghai 200240China institute of Biophysics Chinese Academy of Sciences Beijing100101 China institute of Molecular Medicine Peking University Beijing100871 China College of Engineering Peking University Beiiing 100871 China Laboratory of Disease Models in Non-Human Primates Instituteof Neuroscience Shanghai Chinese Academy of SciencesShanghai 200031 China College of Life sciences Peking University Beijing 100871 China

Dear Editor： Gao et al. published data in Nature Biotechnology （Nat Biotechnol. 2.16 may 2. showing that DNA-guided genome editing using the Natronobacterium gregoryi Argonaute （NgAgo） protein targeted 47 mammalian genomic loci with a 100% success rate and an efficiency of 2..3.-41.3. at various targets. This report led us to test NgAgo＇s utility in various cells and organisms such as mouse and zebrafish for gene editing.

关键词： Supplementary Information Genomic Locus Genome Editing Gene Editing Online Forum

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Biomarkers of aging

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Science china(Life sciences. 2023年第5期66卷 893-1066页

作者： Aging Biomarker Consortium Hainan Bao Jiani Cao Mengting Chen Min Chen wei Chen Xiao Chen Yanhao Chen Yu Chen Yutian Chen Zhiyang Chen Jagadish K Chhetri Yingjie Ding junlin Feng jun Guo Mengmeng Guo Chuting He Yujuan Jia Haiping Jiang Ying Jing Dingfeng Li Jiaming Li Jingyi Li Qinhao Liang Rui Liang Feng Liu Xiaoqian Liu Zuojun Liu Oscar junhong Luo Jianwei Lv Jingyi ma Kehang mao Jiawei Nie Xinhua Qiao Xinpei Sun Xiaoqiang Tang Jianfang Wang Qiaoran Wang Siyuan Wang Xuan Wang Yaning Wang Yuhan Wang Rimo Wu Kai Xia Fu-Hui Xiao Lingyan Xu Yingying Xu Haoteng Yan Liang Yang Ruici Yang Yuanxin Yang Yilin Ying Le Zhang weiwei Zhang Wenwan Zhang Xing Zhang Zhuo Zhang Min Zhou Rui Zhou Qingchen Zhu Zhengmao Zhu Feng Cao Zhongwei Cao Piu Chan Chang Chen Guobing Chen Hou-Zao Chen jun Chen weimin Ci Bi-Sen Ding Qiurong Ding Feng Gao Jing-Dong JHan Kai Huang Zhenyu Ju Qing-Peng kong Ji Li Jian Li Xin Li Baohua Liu Feng Liu Lin Liu Qiang Liu Qiang Liu Xingguo Liu Yong Liu Xianghang Luo Shuai ma Xinran ma Zhiyong mao Jing Nie Yaojin Peng Jing Qu Jie Ren Ruibao Ren Moshi Song Zhou Songyang Yi Eve Sun Yu Sun Mei Tian Shusen Wang Si Wang Xia Wang Xiaoning Wang Yan-Jiang Wang Yunfang Wang Catherine CL Wong Andy Peng Xiang Yichuan Xiao Zhengwei Xie Daichao Xu Jing Ye Rui Yue Cuntai Zhang Hongbo Zhang Liang Zhang weiqi Zhang Yong Zhang Yun-Wu Zhang Zhuohua Zhang {23.biao Zhao Yuzheng Zhao Dahai Zhu weiguo Zou Gang Pei Guang-Hui Liu Aging Biomarker Consortium(ABC) Beijing 100101China CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijing 100101China State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Department of Dermatology Xiangya HospitalCentral South UniversityChangsha 410008China Hunan Key Laboratory of Aging Biology Xiangya HospitalCentral South UniversityChangsha 410008China National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangsha 410008China Clinic Center of Human Gene Research Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Hubei Clinical Research Center of Metabolic and Cardiovascular Disease Huazhong University of Science and TechnologyWuhan 430022China Hubei Key Laboratory of Metabolic Abnor{2.lities and Vascular Aging Huazhong University of Science and TechnologyWuhan 430022China Stem Cell Translational Research Center Tongji HospitalTongji University School of MedicineShanghai 200065China Department of Nuclear Medicine Daping HospitalThird Military Medical UniversityChongqing 400042China CAS Key Laboratory of Nutrition Metabolism and Food SafetyShanghai Institute of Nutrition and HealthUniversity of Chinese Academy of SciencesChinese Academy of SciencesShanghai 200031China Shanghai Key Laboratory of maternal Fetal Medicine Clinical and Translational Research Center of Shanghai First Maternity and Infant HospitalFrontier Science Center for Stem Cell ResearchShanghai Key Laboratory of Signaling and Disease ResearchSchool of Life Sciences and TechnologyTongji UniversityShanghai 200092China The Department of Endovascular Surgery The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China Key Laboratory of Regenerative Medicine of Ministry of Education Institute of Ageing and Regenerative MedicineJ

Aging biomarkers are a combination of biological parameters to(i)assess age-related changes,(ii)track the physiological aging process,and(iii)predict the transition into a pathological *** a broad spectrum of aging biomarkers has been developed,their potential uses and limitations remain poorly *** immediate goal of biomarkers is to help us answer the following three fundamental questions in aging research:How old are we?Why do we get old?And how can we age slower?This review aims to address this ***,we summarize our current knowledge of biomarkers developed for cellular,organ,and organismal levels of aging,comprising six pillars:physiological characteristics,medical imaging,histological features,cellular alterations,molecular changes,and secretory *** fulfill all these requisites,we propose that aging biomarkers should qualify for being specific,systemic,and clinically relevant.

关键词： aging senescence biomarker clock

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Erratum to： Questions about NgAgo

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Protein & Cell 2017年第1期8卷 77-77页

作者： Shawn Burgess Linzhao Cheng Feng Gu junjiu Huang Zhiwei Huang Shuo Lin Jinsong Li wei Li wei Qin Yujie Sun Zhou Songyang wensheng wei Qiang Wu Haoyi Wang Xiaoqun Wang Jing-wei Xiong Jianzhong Xi Hui Yang Bin Zhou bo Zhang National Human Genome Research institute NIH Bethesda MD USA Division of Hematology in Department of Medicine Johns Hopkins University School of Medicine Baltimore MD 21205 USA Stem Cell Program in the institute for Cell Engineering Johns Hopkins University School of Medicine Baltimore MD 21205 USA Center for Vision Research Eye Hospital Wenzhou Medical University Wenzhou 325035 China Stem Cell and Functional Genomics Laboratory School of Life Sciences Sun Yat-sen University Guangzhou 510275 China HIT Center of Life sciences Harbin Institute of Technology Harbin 150080 China Department of Molecular Cell and Developmental Biology University of California Los Angeles Los Angeles CA 90095 USA institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China State Key Laboratory of Stem cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing 100101 China College of Chemical Biology and Biotechnology Peking University Shenzhen Graduate School Shenzhen 518055 China PKU BIOPIC Beijing 100871 China BIOPIC ICG CLS and School of Life Sciences Peking University Beijing 100871 China Center for Comparative Biomedicine Institute of Systems Biomedicine Shanghai Jiao Tong University Shanghai 200240 China institute of Biophysics Chinese Academy of Sciences Beijing 100101 China institute of Molecular Medicine Peking University Beijing 100871 China College of Engineering Peking University Beijing 106871 China Laboratory of Disease Models in Non-Human Primates Institute of Neuroscience Shanghai Chinese Academy of Sciences Shanghai 200031 China College of Life sciences Peking University Beijing 100871 China

In the original publication of this article the Figure 1E legend has been incorrectly published.

关键词： Erratum Questions NgAgo

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Structure and degradation of circular RNAs regulate PKR activation in innate immunity

Structure and degradation of circular RNAs regulate PKR acti...

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中国生物化学与分子生物学会2.19年全国学术会议暨学会成立四十周年

作者： Chu-Xiao Liu Xiang Li Fang Nan Shan Jiang Xiang Gao Si-Kun Guo wei Xue Yange Cui Kaige Dong Huihua Ding bo Qu Zhaocai Zhou Nan Shen Li Yang Ling-Ling Chen State Key Laboratory of Molecular Biology Shanghai Key Laboratory of Molecular Andrology CAS Center for Excellence in Molecular Cell Science Shanghai Institute of Biochemistry and Cell Biology University of Chinese Academy of Sciences Chinese Academy of Sciences Key Laboratory of Computational Biology CAS-MPG Partner Institute for Computational Biology Shanghai Institute of Nutrition and Health Shanghai Institutes for Biological Sciences University of Chinese Academy of Sciences Chinese Academy of Sciences school of Life Science and Technology ShanghaiTech University Laboratory of Molecular Rheumatology Shanghai Institute of Nutrition and Health Shanghai Institutes for Biological Sciences University of Chinese Academy of Sciences Chinese Academy of Sciences Shanghai institute of Rheumatology China-Australia Centre for Personalized Immunology Renji Hospital School of Medicine Shanghai Jiao Tong University Center for Autoimmune Genomics and Etiology Cincinnati Children’s Hospital Medical Center Department of Pediatrics University of Cincinnati College of Medicine

CircRNAs produced from back-splicing of exons of pre-mRNAs are widely expressed, but current understanding of their functions is limited. These RNAs are stable in general and are thought to have unique structural conformations distinct from their linear RNA cognates. Here we uncover that endogenous circRNAs tend to form 16-2. bp imperfect RNA duplexes and act as inhibitors of double-stranded RNA(dsRNA)-activated protein kinase(PKR) related to innate immunity. Upon poly(I:C) stimulation or viral infection, circRNAs are globally degraded by RNase L, a process required for PKR activation in early cellular innate immune responses. Augmented PKR phosphorylation and circRNA reduction are found in peripheral blood mononuclear cells(PBMCs) derived from patients of autoimmune disease systemic lupus erythematosus(SLE). Importantly, over-expression of the dsRNA-containing circRNA in PBMCs or T cells derived from SLE can alleviate the aberrant PKR activation cascade, thus providing a connection between circRNAs and SLE.

关键词： Circular RNAs(circRNAs) circRNA structure circRNA degradation double-stranded RNAs(dsRNAs) RNase L PKR innate immune responses autoimmune disease SHAPE-maP systemic lupus erythematosus(SLE)

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Abstracts from the 15th International Myopia Conference

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Eye and Vision 2016年第1期3卷 211-221页

作者： Alexandra Benavente-Perez Ann Nour Tobin Ansel Kathleen Abarr Luying Yan Keisha Roden David Troilo Chanyi Lu Miaozhen Pan Min Zheng Jia Qu Xiangtian Zhou Christine F. Wildsoet Fan Lu Jie Chen Jinhua Bao Liang Hu Qinmei Wang Zibing Jin Frances Rucker Stephanie Britton Stephan Hanowsky Molly Spatcher Hui-Ying Kuo Ching-Hsiu Ke I-Hsin Kuo Chien-Chun Peng Han-Yin Sun Ian G. Morgan Jeremy A. Guggenheim Rupal L. Shah Cathy Williams Jinglei Yang Peter S. Reinach Sen Zhang Wenfeng Sun bo Liu Fen Li Xiaoqing Li Aihua Zhao Tianlu Chen wei Jia {2. Jiang Haoran Wu Kazuo Tsubota Hiroko Ozawa Hidemasa Torii Shigemasa Takamizawa Toshihide Kurihara Kazuno Negishi Klaus Graef Daniel Rathbun Frank Schaeffel Ladan Ghodsi William K. Stell machelle T. Pardue Ranjay Chakraborty Han na Park Curran S. Sidhu P. Michael Iuvone Michael J Collins Nethrajeith Srinvasalu Sally A. McFadden Paul N. Baird Pablo Artal Pauline Cho SW Cheung Pei-Chang Wu Quan V. Hoang Duk C. Lee Erica G. Landis Michael A. Bergen Curran Sidhu Samer Hattar Richard A. Stone Ravi Metlapally Ruiqin Li Qinglin Xu Hong Zhong Chenglin Pan weizhong Lan Xiaoning Li Ling Chen Zhikuan Yang Scott A. Read Seang-Mei Saw Shi-{2. Weng Xiao-Hua Wu Kang-wei Qian Yun-Yun Li Guo-Zhong Xu Furong Huang Xiong-Li Yang Yong-Mei Zhong Earl L Smith, III Baskar Arumugam Li-Fang Hung Lisa A. Ostrin Klaus Trier Monica Jong Brien A. Holden Thomas Chuen Lam Samantha Shan Bing Zuo Dennis Yan-yin Tse Jingfang Bian King-Kit Li Quan Liu Chi-ho To Timothy J. Gawne John T. Siegwart, Jr. Alexander H. Ward Thomas T. Norton Yan Zhang Yue Liu Carol Ho Eileen Phan Abraham Hang Emily Eng Christine Wildsoet Department of biological sciences SUNY College of Optometry New York NY 10036 USA school of Optometry and Ophthalmology and Eye Hospital Wenzhou Medical University Wenzhou Zhejiang 325027 China Center for Eye Disease & Development School of Optometry & Vision Science Program University of California Berkeley Berkeley CA 94720-2020 USA Wenzhou medical university Wenzhou Zhejiang 325027 China Biomedical Science and Disease New England College of Optom Boston MA USA school of Optometry Chung Shan Medical University Taichung City 402 Taiwan institute of Medicine Chung Shan Medical University Taichung City 402 Taiwan Division of Biochemistry and Molecular Biology Research School of Biology Australian National University Canberra Australia State Key Laboratory of Ophthalmology and Division of Preventive Ophthalmology Zhongshan Ophthalmic Center Sun Yat-Sen University Guangzhou China school of Optometry & Vision sciences Cardiff University Cardiff UK school of Social and Community Medicine University of Bristol Bristol UK school of and Eye Hospital Wenzhou Medical University Wenzhou Zhejiang 325027 China State Key Laboratory Cultivation Base and Key Laboratory of Vision Science Ministry of Health and Zhejiang Provincial Key Laboratory of Ophthalmology and Optometry Wenzhou Zhejiang 325027 People’s Republic of China Shanghai Key Laboratory of Diabetes Mellitus and Center for Translational Medicine Shanghai Jiao Tong University Affiliated Sixth People’s Hospital Shanghai 200233 China Eye Hospital of Wenzhou medical university Wenzhou Zhejiang 325027 China Department of Ophthalmology Keio University School of Medicine Shinjuku-ku Tokyo 160-8582 Japan General Medicine Ichikawa Medical Center Urayasu City Chiba 279-0001 Japan Ophthalmic Research institute University of Tuebingen 72076 Tuebingen Germany Bernstein Center for Computational Neuroscience Tuebingen 72076 Tuebingen Germany Werner Reichardt Centre for Integrative Neuroscience Otfried-Mueller-Straße 25 Tueb

To assess the peripheral refractive changes that occur when eye growth decelerates in *** We measured peripheral refraction and on-axis vitreous chamber depth on a total of 53.marmosets;3. treated monocularly for 12.weeks 9 hrs/day(2. with-5 D contact lenses,10 with+5 D)and 17 untreated *** the 2. marmosets treated with-5 D,10 wore contact lenses 9 hrs/day without interruptions and were mea-sured at the end of 10 weeks of treatment(T10)and after 4 weeks of recovery(R14).Eight had {2.th contact lenses removed for 3. mins twice/day(mid-morning and mid-afternoon)during the first four weeks of treatment(early interruption group)and eight had the lenses removed during the second four weeks of treatment(late interruption group).The two interruption groups were measured after 4,8 and 12.weeks of treatment only(T4,T8,T12..

关键词： removed treatment untreated

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