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Improved cryopreservation of human hepatocytes using a new xeno free cryoprotectant solution

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World Journal of Hepatology 2012年第5期4卷 176-183页

作者： Mohammed Saliem Frida Holm Rosita Bergstrm Tengzelius Carl Jorns Lisa-Mari Nilsson bo-gran ericzon Ewa Ellis Outi Hovatta Division of Obstetrics and gynecology Department of Clinical ScienceIntervention and TechnologyKarolinska Institute141 86 StockholmSweden Division of Transplantation Surgery Department of Clinical ScienceIntervention and TechnologyKarolinska Institute141 86 StockholmSweden Division of Pediatrics Department of Clinical ScienceIntervention and TechnologyKarolinska Institute141 86 StockholmSweden

AIM:To optimize a xeno-free cryopreservation protocol for primary human ***:The demand for cryopreserved hepatocytes is increasing for both clinical and research *** several hepatocyte cryopreservation protocols being available,improvements are urgently *** first compared controlled rate freezing to polystyrene box freezing and did not find any significant change between the *** the polystyrene box freezing,we compared two xeno-free freezing solutions for freezing of primary human hepatocytes:a new medium(STEM-CELLBANKER,CB),which contains dimethylsulphoxide(DMSO) and anhydrous dextrose,both permeating and non-permeating cryoprotectants,and the frequently used DMSO-University of Wisconsin(DMSOUW) *** viability of the hepatocytes was assessed by the trypan blue exclusion method as well as a calcein-esterase based live-dead assay before and after *** function of the hepatocytes was evaluated before and after cryopreservation by assessing enzymatic activity of 6 major cytochrome P450 isoforms(CYPs):CYP1A2,CYP2C9,CYP2C19,CYP2D6,CYP3A4 and ***:The new cryoprotectant combination preserved hepatocyte viability significantly better than the standard DMSO-UW protocol(P gnificant difference in viability estimation between both the trypan blue(TB) and the Live-Dead Assay *** was a correlation between viability of fresh hepatocytes and the difference in cell viability between CB and DMSO protocols(r 2 = 0.69) using the TB ***,due to high within-group variability in the activities of the major CYPs,any statistical between-group differences were *** of human hepatocytes using the cryoprotectant combination was a simple and xeno-free procedure yielding better hepatocyte ***,it may be a better alternative to the standard DMSO-UW *** CYP activities did not seem to be a relevant way to compare hepatocyte function

关键词： Human hepatocytes Viability Cytochrome P540 Dimethylsulphoxide Cryoprotectant Cryopreservation

Symptomatic Val122del mutated hereditary transthyretin amyloidosis: Need for early diagnosis and prioritization for heart and liver transplantation

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Hepatobiliary & Pancreatic Diseases International 2021年第4期20卷 323-329页

作者： Adriano-Valerio Schettini Laura Llado JulieK Heimbach Josegonzalez Costello Marie Tranäng Olivier Van Caenegem Richard C Daly Peter Van den Bergh Carlos Casasnovas Joan Fabregat John J Poterucha Maxime Foguenne bo göran ericzon Jan Lerut Institute for Experimental and Clinical Research(IREC) Catholic University of Louvain(UCL)Avenue Hippocrate 55Brussels 1200Belgium Liver Transplant Unit Department of SurgeryHospital Universitari de BellvitgeCalle de la Feixa LlargaBarcelona 08907Spain Division of Transplantation Surgery William J.von Liebig Transplant CenterMayo Clinic2001st St SWRochesterMN 55905USA Advanced Heart Failure and Transplant Unit Cardiology DepartmentHospital Universitari de BellvitgeCalle de la Feixa LlargaBarcelona 08907Spain Familial Amyloidotic Polyneuropathy World Transplant Registry(FAPWTR) Division of Transplantation SurgeryKarolinska Universitetssjukhuset HuddingeF82Karolinska InstitutetStockholm 14186Sweden Cardiothoracic Intensive Care Unit Department of Cardiovascular DiseasesUniversity Hospitals Saint-Luc-UCLAvenue Hippocrate 10Brussels 1200Belgium Cardiovascular Surgery Department Mayo Clinic2001st St SWRochesterMN 55905USA Neuromuscular Reference Centre University Hospital Saint-Luc-UCLAvenue Hippocrate 10Brussels 1200Belgium Neuromuscular Unit Neurology DepartmentHospital Universitari de BellvitgeCalle de la Feixa LlargaBarcelona 08907Spain Division of gastroenterology and Hepatology Mayo Clinic2001st St SWRochester MN 08907USA Division of Transplantation Surgery CLINTECKarolinska Universitetssjukhuset HuddingeF82Karolinska InstitutetStockholm 14186Sweden

Background: Hereditary transthyretin(ATTRv) amyloidosis is an autosomal dominant disease linked to transthyretin gene mutations which cause instability of the transthyretin tetramer. After dissociation and misfolding they reassemble as insoluble fibrils(i.e. amyloid). Apart from the common Val30 Met mutation there is a very heterogeneous group of non-Val30 Met mutations. In some cases, the clinical picture is dominated by a rapidly evolving restrictive and hypertrophic cardiomyopathy. Methods: A case series of four liver recipients with the highly clinically relevant, rare and particularly aggressive Val122 del mutation is presented. Medical and surgical therapeutic options, waiting list policy for ATTRv-amyloidosis, including the need for heart transplantation, and status of heart-liver transplantation are discussed. Results: Three patients needed a staged(1 patient) or simultaneous(2 patients) heart-liver transplant due to rapidly progressing cardiac failure and/or neurologic disability. Domino liver transplantation was impossible in two due to fibrotic hepatic transformation caused by cardiomyopathy. After a follow-up ranging from 3.5 to 9.5 years, cardiac(allograft) function was maintained in all patients, but neuropathy progressed in three patients, one of whom died after 80 months. Conclusions: This is the first report in(liver) transplant literature about the rare Val122 del ATTRv mutation. Due to its aggressiveness, symptomatic patients should be prioritized on the liver and, in cases with cardiomyopathy, heart waiting lists in order to avoid the irreversible neurological and cardiac damage that leads to a rapid lethal outcome.

关键词： Hereditary transthyretin amyloidosis Heart transplantation Liver transplantation Non-Val30Met mutation Val122del mutation Domino liver transplantation

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66例原位肝脏移植及围手术期处理分析

华西医科大学学报 2002年第4期33卷 661-663页

作者：伍晓汀严律南 Carl-gustav groth bo-gran ericzon 周勇庄文张明鸣四川大学华西医院普通外科成都610041 Department of Transplantation Surgery Huddinge University HospitalKarolins ka Institute

目的　总结 6 6例肝移植及围手术期处理的经验 ,探讨适合我国目前及今后提高肝移植成活率和康复率的方法。方法　 6 6例接受肝移植手术患者 ,12例行劈离式肝移植 (SL T)术 ,5 4例行原位肝移植 (OL T)术。结果　移植的 6 6例肝脏 ,术后... 详细信息

目的　总结 6 6例肝移植及围手术期处理的经验 ,探讨适合我国目前及今后提高肝移植成活率和康复率的方法。方法　 6 6例接受肝移植手术患者 ,12例行劈离式肝移植 (SL T)术 ,5 4例行原位肝移植 (OL T)术。结果　移植的 6 6例肝脏 ,术后无 1例发生肝脏功能衰竭 ,也无重大并发症发生 ,病人全部存活 ,多数已康复。结论　改善供肝条件 ,严格受体选择 ,提高手术水平。

关键词：肝脏移植围手术期处理免疫抑制剂

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Molecular mechanism and research progress of traditional Chinese medicine in the treatment of prostate cancer

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Traditional Medicine Research 2025年第1期10卷 62-72页

作者： Hai-Luo Wang Ruo-ran Zhang Hao Wang Peng Xu Jing-Kai Wang bo Chen Hao Xu Deng Pan Yu-Yang Ma Kun Pang Department of Urology Xuzhou Central HospitalXuzhou Clinical School of Xuzhou Medical CollegeXuzhou 221009China graduate School Jiangsu UniversityZhenjiang 212013China graduate School Bengbu Medical UniversityBengbu 233040China Department of Urology The Peixian People’s HospitalXuzhou 221699China.

Prostate cancer(PCa)is one of the most common malignant tumors in the male genitourinary system,ranking second in incidence *** Chinese medicine(TCM),as an important component of complementary and alternative medicine,shows unique advantages in cancer *** herbal medicine is usually composed of multiple ingredients and involves multiple signaling pathways,which showed function of inducing apoptosis of cancer cells,arresting the cell cycle,inhibiting invasion and metastasis,reducing drug resistance,and regulating immune *** therapy is also an important treatment of ***,Physical therapy such as acupuncture or Tai Chi and Qigong are gaining increased recognition in the management of PCa,particularly in addressing issues like urinary incontinence and bone metastasis-related *** article reviews the TCM treatment and therapy of PCa,in order to provide new research avenues and treatment options for the treatment of PCa with TCM and improve the quality of life of patients.

关键词： traditional Chinese medicine prostate cancer Chinese herbal medicine physical therapy

Traffic safety helmet wear detection based on improved YOLOv5 network

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Optoelectronics Letters 2025年第1期21卷 35-42页

作者： gUI Dongdong SUN bo College of Software Southeast University Quanzhou Equipment Research Centre Haixi Institute Chinese Academy of Sciences

Aiming at the problem that the current traffic safety helmet detection model can't balance the accuracy of detection with the size of the model and the poor generalization of the model, a method based on improving you only look once version 5(YOLOv5) is proposed. By incorporating the lightweight ghost Net module into the YOLOv5 backbone network, we effectively reduce the model size. The addition of the receptive fields block(RFB) module enhances feature extraction and improves the feature acquisition capability of the lightweight model. Subsequently, the high-performance lightweight convolution, gSConv, is integrated into the neck structure for further model size compression. Moreover, the baseline model's loss function is substituted with efficient insertion over union(EIoU), accelerating network convergence and enhancing detection precision. Experimental results corroborate the effectiveness of this improved algorithm in real-world traffic scenarios.

关键词： Traffic control

Mechanism of action of Linggui Zhugan Decoction in treating non-alcoholic fatty liver disease using untargeted metabolomics approach

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Traditional Medicine Research 2025年第2期10卷 65-76页

作者： Huan Pei Ren-Lin Li Xin-ran Song Qian-Qian Wan Yu-Ming Wang Han-Zhou Li Wei-Quan Xu Jia-Bao Liao Wei-bo Wen Jing Miao Huan-Tian Cui School of Basic Medicine Yunnan University of Chinese MedicineKunming 650599China School Clinical Medical College Yunnan University of Chinese MedicineKunming 650599China First School of Clinical Medicine Yunnan University of Chinese MedicineKunming 650599China School of Integrative Chinese and Western Medicine Tianjin University of Traditional Chinese MedicineTianjin 301617China Department of Pharmacy Tianjin Second People's HospitalTianjin 300192China

Background:Non-alcoholic fatty liver disease(NAFLD)is a liver disorder characterized by the accumulation and degeneration of fat in the liver cells,a condition that may further deteriorate and lead to cirrhosis and liver *** studies showed that metabolic dysfunction can promote NAFLD *** Zhugan Decoction(LgZgD)has therapeutic effects on *** mechanism of LgZgD still remains *** study was to examine the impact of LgZgD on the metabolic processes involved in the development of ***:A mice model of NAFLD was treated with *** therapeutic potential of LgZgD was evaluated by assessing the activity of transaminases,lipids levels of blood,and pathological changes in the liver of the mice model of ***,this study also evaluated the influence of LgZgD on liver inflammation and oxidative ***:The results of untargeted metabolomics analysis showed that LgZgD reduced the disordered lipid metabolism in NAFLD *** improved the oxidative stress and also reduced the levels of pro-inflammatory cytokines in the *** metabolomics analysis of liver samples revealed that LgZgD treatment improved metabolic disorders,including alanine,aspartate,glutamate,glycerophospholipid metabolism,and citrate *** RT-qPCR and Western blot results showed that LgZgD could regulate the expression of key enzymes in the metabolic pathway of the citrate cycle,including ATP-citrate lyase(ACLY),alanine-glyoxylate aminotransferase-2(AgXT2),phosphatidylethanolamine N-methyltransferase(PEMT),and succinate dehydrogenase(SDH).Conclusion:We found that LgZgD can treat NAFLD by reducing inflammatory responses,inhibiting oxidative stress,regulating alanine,aspartate,glutamate,and glycerophospholipid metabolism,and citrate cycle pathways.

关键词： NAFLD LgZgD untargeted metabolomics glycerophospholipid metabolism citrate cycle

Correlation between anxiety, depression, and social stress in young patients with thoracolumbar spine fractures

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World Journal of Psychiatry 2025年第1期15卷 83-92页

作者： bo Wang Da Shi Yin-Di Sun bo Dong Joint Ward of Orthopedics Department of TCM Honghui HospitalXi'an Jiaotong UniversityXi’an 710054Shaanxi ProvinceChina Pain Ward of Orthopedics Department of TCM Honghui HospitalXi'an Jiaotong UniversityXi’an 710054Shaanxi ProvinceChina

BACKgROUND Traumatic injuries,such as falling,car accidents,and crushing mostly cause spinal fractures in young and middle-aged people,and>50%of them are thoracolumbar *** kind of fracture is easily combined with serious injuries to peripheral nerves and soft tissues,which causes paralysis of the lower limbs if there is no timely rehabilitation *** patients with thoracolumbar fractures find it difficult to recover after the operation,and they are prone to depression,low self-esteem,and other negative *** To investigate the association between anxiety,depression,and social stress in young patients with thoracolumbar spine fractures and the effect on rehabilitation *** This study retrospectively analyzed 100 patients admitted to the orthopedic department of Honghui Hospital,Xi’an Jiaotong University who underwent thoracolumbar spine fracture surgery from January 2022 to June *** general data of the patients were assessed with the Hamilton anxiety scale(HAMA),Hamilton depression scale(HAMD),life events scale,and social support rating scale(SSRS)to identify the correlation between anxiety,depression scores,and social stress and social *** Japanese Orthopedic Association(JOA)was utilized to evaluate the rehabilitation outcomes of the patients and to analyze the effects of anxiety and depression scores on *** According to the scores of HAMD and HAMA in all patients,the prevalence of depression in patients was 39%(39/100),and the prevalence of anxiety was 49%(49/100).Patients were categorized into non-depression(n=61)and depression(n=39),non-anxiety(n=51),and anxiety(n=49)*** significant differences in gender,occupation,Pittsburgh Sleep Quality Index(PSQI)score,and monthly family income were observed between the non-depression and depression groups(Pgnificant difference in occupation and PSQI score was found between the non-anxiety and anxiety *** depression(r=0

关键词： Patients with thoracolumbar fractures Anxiety Depression Social pressure Social support

Effects of aggregate size distribution and carbon nanotubes on the mechanical properties of cemented gangue backfill samples under true triaxial compression

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 311-324页

作者： Qian Yin Fan Wen Zhigang Tao Hai Pu Tianci Deng Yaoyao Meng Qingbin Meng Hongwen Jing bo Meng Jiangyu Wu State Key Laboratory of Intelligent Construction and Healthy Operation and Maintenance of Deep Underground Engineering China University of Mining and TechnologyXuzhou 221116China State Key Laboratory of Mining Response and Disaster Prevention and Control in Deep Coal Mines Anhui University of Science and TechnologyHuainan 232001China State Key Laboratory for geomechanics&Deep Underground Engineering China University of Mining and Technology(Beijing)Beijing 100083China Key Laboratory of Xinjiang Coal Resources green Mining Ministry of EducationXinjiang Institute of EngineeringUrumqi 830023China

The mechanical behavior of cemented gangue backfill materials(CgBMs)is closely related to particle size distribution(PSD)of aggregates and properties of cementitious ***,the true triaxial compression tests,CT scanning,SEM,and EDS tests were conducted on cemented gangue backfill samples(CgBSs)with various carbon nanotube concentrations(P_(CNT))that satisfied fractal theory for the PSD of *** mechanical properties,energy dissipations,and failure mechanisms of the CgBSs under true triaxial compression were systematically *** results indicate that appropriate carbon nanotubes(CNTs)effectively enhance the mechanical properties and energy dissipations of CgBSs through micropore filling and microcrack bridging,and the optimal effect appears at P_(CNT)of 0.08wt%.Taking PSD fractal dimension(D)of 2.500 as an example,compared to that of CgBS without CNT,the peak strength(σ_(p)),axial peak strain(ε_(1,p)),elastic strain energy(Ue),and dissipated energy(U_(d))increased by 12.76%,29.60%,19.05%,and90.39%,***,excessive CNTs can reduce the mechanical properties of CgBSs due to CNT agglomeration,manifesting a decrease inρ_(p),ε_(1,p),and the volumetric strain increment(Δε_(v))when P_(CNT)increases from 0.08wt%to 0.12wt%.Moreover,the addition of CNTs improved the integrity of CgBS after macroscopic failure,and crack extension in CgBSs appeared in two modes:detour and pass through the ***σ_(p)and U_(d)firstly increase and then decrease with increasing D,and porosity shows the opposite ***ε_(1,p)andΔε_(v)are negatively correlated with D,and CgBS with D=2.150 has the maximum deformation parameters(ε_(1,p)=0.05079,Δε_(v)=0.01990)due to the frictional slip effect caused by coarse *** increasing D,the failure modes of CgBSs are sequentially manifested as oblique shear failure,"Y-shaped"shear failure,and conjugate shear failure.

关键词： cemented gangue backfill materials particle size distribution true triaxial compression test carbon nanotubes mechanical properties failure modes

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Sky location of massive black hole binaries in the foreground of galactic white dwarf binaries

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 97-108页

作者： Pan guo Hong-bo Jin Cong-Feng Qiao Yue-Liang Wu The International Centre for Theoretical Physics Asia-Pacific University of Chinese Academy of Sciences The School of Physical Sciences University of Chinese Academy of Sciences Institute for Frontiers in Astronomy and Astrophysics Beijing Normal University National Astronomical Observatories Chinese Academy of Sciences Institute of Theoretical Physics Chinese Academy of Sciences

For space-based gravitational wave （gW） detection,the main noise source for massive black hole binaries （MBHBs） is attributed to approximately 10～7double white dwarf binaries in the *** a gW source,the amplitude of the detector response,recorded by a space-based gravitational wave detector,exhibits a modulation effect with a year period when observing the source from various orbital *** the adverse conditions mentioned above,where there is a strong foreground noise and annual modulation in the signals,we employed the wavelet transform and the strong-amplitude relevant orbital position search methods,which allows the weak MBHB sources to achieve higher locating *** detail,for two MBHB sources of lower intensity,the precision of luminosity distance,represented by the ratio ?DL/DLat the 95%confidence level,is enhanced by factors of ～*** angular resolutions,denoted by??s,are enhanced by a factor of ～*** improvements increase the number of detectable gW sources,facilitate multi-messenger follow-up observations and provide constraints on the cosmological constant.

关键词： space-based gravitational wave detection gravitational waves massive black hole binaries