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Curcumin induces apoptosis through the mitochondria-mediated apoptotic pathway in HT-29 cells

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第2期10卷 93-102页

作者： jin-bo WANG Li-li QI Shui-di ZHENG Tian-xing WU Department of Chemistry College of Science Zhejiang University Hangzhou 310027 China Ningbo Institute of Technology Zhejiang University Ningbo 315100 China

Objective： To investigate the effects of curcumin on release of cytochrome c and expressions of Bcl-2, Bax, Bad, Bcl-xL, caspase-3, poly ADP-ribose polymerase （PARP）, and survivin of HT-29 cells. Methods： HT-29 cells were treated with curcumin （0-80 μmol/L） for 24 h. The release of cytochrome c from the mitochondria and the apoptosis-related proteins Bax, Bcl-2, Bcl-xL, Bad, caspase-3, PARP, and survivin were determined by Western blot analysis and their mRNA expressions by reverse transcriptase-polymerase chain reaction （RT-PCR）. Results： Curcumin significantly induced the growth inhibition and apoptosis of HT-29 cells. A decrease in expressions of Bcl-2, Bcl-xL and survivin was observed after exposure to 10-80 μmol/L curcumin, while the levels of Bax and Bad increased in the curcumin-treated cells. Curcumin also induced the release of cyto- chrome c, the activation ofcaspase-3, and the cleavage of PARP in a dose-dependent manner. Conclusion： These data suggest that curcumin induced the HT-29 cell apoptosis possibly via the mitoehondria-mediated pathway.

关键词： Curcumin Apoptosis Mitochondrial pathway HT-29 cells

Pharmacokinetic analysis and tissue distribution of Vam3 in the rat by a validated LC-MS/MS method

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Acta Pharmaceutica Sinica B 2015年第3期5卷 254-263页

作者： Ruixia Zhang Ping Mao Tingting Zhang Chen Ma bo jin Tong Li Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College

Vam3 is a potential pharmacologically active ingiedient isolated from Vitis amurensis Rupr. A rapid, simple and sensitive method to determine Vam3 levels in rat plasma and tissue was developed based on EC-MS/MS. Vam3 and an internal standard (IS) were chromatographed on a C18 short column with acetonitrile-0.1% formic acid in water by gradient elution. MS detection was performed by electrospray ionization in negative ion multiple reaction monitoring modes,This method monitored the transitions m/z 451.0 -> 345.0 and m/z 301.0 -> 164.0 for Vam3 and IS, respectively. The calibration curve was linear over a concentration range of 1,64-1000 ng/mE, The inter-day and intra-day variabilities in precision was less than 12.8%, while the inter -day and intim-day accuracies ranged from 10.60% to 9.084 in plasma and tissue homogenates. This method was applied to investigate the pharmacokinetics and tissue distribution of Vain3 in rats. The results indicated that Vain3 had poor absorption into systemic circulation and extensive tissue distribution after oral administration, and the absolute bioavailability was low (0.79%). Vam3 had a relatively long terminal elimination half-life in lung, and the highest concentration was found in small intestinal tissue. The developed method and the pharmacokinetic data can provide a basis for further studies on the bioactivity of Vam3. (C) 2015 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V.

关键词： Vam3 Pharmacokinetics Tissue distribution Absolve bioavialability LC-MS/MS

Inhibiting shuttle effect of lithium polysulfides by double metal selenides for high-performance lithium-sulfur batteries

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Rare Metals 2024年第6期43卷 2546-2559页

作者： Lei Li Xue-jing Yang Yi-Yang Li bo jin Hui Liu Meng-Yang Cui Dong-bo Guan Xing-You Lang Qing Jiang Key Laboratory of Automobile Materials Ministry of Educationand College of Materials Science and EngineeringJilin UniversityChangchun 130022China

Lithium-sulfur batteries(LSBs)have attracted the attention of more and more researchers due to the advantages of high energy density,environmental friendliness,and low production ***,the low electronic conductivity of active material and shuttling effect of lithium polysulfides(LiPSs)limit the commercial development of *** solve these problems,we design a core-shell composite with nitrogen-doped carbon(NC)and two types of selenides(FeSe_(2)-NC@ZnSe-NC).The FeSe_(2)-NC@ZnSe-NC has a strong adsorption capacity,and can effectively adsorb *** the same time,it also effectively alleviates the shuttling effect of LiPSs,and improves the utilization of the active substance during the charge/discharge reaction *** mechanism involved in FeSe_(2)-NC@ZnSe-NC is demonstrated by both experiments and density-functional theory(DFT)*** electrochemical test results indicate that LSB with S/FeSe_(2)-NC@ZnSe-NC delivers an initial discharge capacity of 1260 mAh·g^(-1)at *** after 500 cycles at 1C,the capacity decay rate per cycle is 0.031%,and the capacity retention rate is 85%.The FeSe_(2)-NC@ZnSe-NC core-shell structure verifies a rational strategy to construct an electrode material for high-performance LSBs.

关键词： Lithium-sulfur batteries Shuttling effect Metal selenide Nitrogen-doped carbon Lithium polysulfides

Clinical and genomic analysis of baseline and acquired MET fusions with an intact kinase domain in lung cancer patients

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Genes & Diseases 2024年第1期11卷 76-79页

作者： bo jin Yutong Ma Qian Wu Qiuxiang Ou Yang Shao Shun Xu Department of Medical Oncology The First Hospital of China Medical UniversityShenyangLiaoning 110001China Geneseeq Research Institute Nanjing Geneseeq Technology Inc.NanjingJiangsu 210000China School of Public Health Nanjing Medical UniversityNanjingJiangsu 211166China Department of Thoracic Surgery The First Hospital of China Medical UniversityShenyangLiaoning110001China

MET gene alterations in lung cancer patients mainly include exon 14 skipping and gene amplification,which are the key therapeutic targets and drive resistance to tyrosine kinase inhibitors(TKls).1 However,the structural variants of MET,such as MET fusions,are much rarer(0.26%),as reported in a Chinese non-small cell lung cancer(NSCLC)cohort.2 Several recurrent MET fusions,such as KIF5B-MET and HLADRB1-MET,were reported as oncogenic drivers and showed favorable responses to crizotinib.

关键词： lung patients alterations

High-performance size exclusion chromatography with a multi-wavelength absorbance detector study on dissolved organic matter characterisation along a water distribution system

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Journal of Environmental Sciences 2016年第6期28卷 235-243页

作者： Huiping Huang Emma Sawade David Cook Christopher W.K.Chow Mary Drikas bo jin School of Chemical Engineering The University of Adelaide Australian Water Quality Centre SA Water Corporation250 Victoria Square School of Earth and Environmental Sciences The University of Adelaide

This study examined the associations between dissolved organic matter（DOM） characteristics and potential nitrification occurrence in the presence of chloramine along a drinking water distribution system. High-performance size exclusion chromatography（HPSEC） coupled with a multiple wavelength detector（200–280 nm） was employed to characterise DOM by molecular weight distribution, bacterial activity was analysed using flow cytometry, and a package of simple analytical tools, such as dissolved organic carbon, absorbance at 254 nm, nitrate,nitrite, ammonia and total disinfectant residual were also applied and their applicability to indicate water quality changes in distribution systems were also evaluated. Results showed that multi-wavelength HPSEC analysis was useful to provide information about DOM character while changes in molecule weight profiles at wavelengths less than 230 nm were also able to be related to other water quality parameters. Correct selection of the UV wavelengths can be an important factor for providing appropriate indicators associated with different DOM compositions. DOM molecular weight in the range of 0.2–0.5 kDa measured at210 nm correlated positively with oxidised nitrogen concentration（r = 0.99）, and the concentrations of active bacterial cells in the distribution system（r = 0.85）. Our study also showed that the changes of DOM character and bacterial cells were significant in those sampling points that had decreases in total disinfectant residual. HPSEC-UV measured at210 nm and flow cytometry can detect the changes of low molecular weight of DOM and bacterial levels, respectively, when nitrification occurred within the chloraminated distribution system.

关键词： HPSEC-UV Distribution system DOM Sum of nitrate and nitrite concentration

A long-term in situ calibration system for chemistry analysis of seawater

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2010年第9期11卷 701-708页

作者： Chun-yang TAN bo jin Kang DING William E. SEYFRIED Jr. Ying CHEN State Key Lab of Fluid Power Transmission and Control Zhejiang University Hangzhou 310027 China Department of Geology and Geophysics University of Minnesota Minneapolis 55455 USA

An in situ calibration system is a versatile exploration instrument for electrochemical sensors investigating the biochemical properties of the marine environment. The purpose of this paper is to describe the design of an auto-calibrating system for electrochemical (pH) sensors, which permits two-point in situ calibration, suitable for long-term measurement in deep sea aqueous environments. Holding multiple sensors, the instrument is designed to perform long-term measurements and in situ calibrations at abyssal depth (up to 4000 m). The instrument is composed of a compact fluid control system which is pressure-equilibrated and designed for deep-sea operation. In situ calibration capability plays a key role in the quality and reproducibility of the data. This paper focuses on methods for extending the lifetime of the instrument, considering the fluidics design, mechanical design, and low-power consumption of the electronics controller. The instrument can last 46 d under normal operating conditions, fulfilling the need for long-term operation. Data concerning pH measured during the KNOX18RR cruise (Mid-Atlantic Ridge, July-August, 2008) illustrate the desirable properties of the instrument. Combined with different electrodes (pH, H2, H2S, etc.), it should be of great utility for the study of deep ocean environments, including water column and diffuse-flow hydrothermal fluids.

关键词： pH Long-term In situ calibration Flow control Low power

Hidden Tree Structure is a Key to the Emergence of Scaling in the World Wide Web

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Chinese Physics Letters 2011年第1期28卷 218-221页

作者： ZHENG bo-jin WANG Jian-Min CHEN Gui-Sheng JIANG Jian SHEN Xian-Jun School of Software Tsinghua University Beijing 100084 College of Computer Science South-Central University for Nationalities Wuhan 430074 Institute of Chinese Electronic Engineering Beijing 100840 Institute of Command and Technology of Equipment Beijing 101416 Department of Computer Science Central-China Normal University Wuhan 430072

Preferential attachment is the most popular explanation for the emergence of scaling behavior in the World Wide Web, but this explanation has been challenged by the global information hypothesis, the existence of linear preference and the emergence of new big internet companies in the real world. We notice that most websites have an obvious feature that their pages are organized as a tree （namely hidden tree） and hence propose a new model that introduces a hidden tree structure into the Erd6s^R~nyi model by adding a new rule： when one node connects to another, it should also connect to all nodes in the path between these two nodes in the hidden tree. The experimental results show that the degree distribution of the generated graphs would obey power law distributions and have variable high clustering coefficients and variable small average lengths of shortest paths. The proposed model provides an alternative explanation to the emergence of scaling in the World Wide Web without the above-mentioned difficulties, and also explains the ＂preferential attachment＂ phenomenon.

关键词： Statistical physics and nonlinear systems

IEEE 1588 based time synchronization system for a seafloor observatory network

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Journal of Zhejiang University-Science C(Computers and Electronics) 2013年第10期14卷 766-776页

作者： De-jun LI Gang WANG Can-jun YANG bo jin Yan-hu CHEN State Key Laboratory of Fluid Power Transmission and Control Zhejiang University

An IEEE 1588 based application scheme was proposed to achieve accurate time synchronization for a deep seafloor observatory network based on the communication topological structure of the Zhejiang University Experimental and Research *** principles of the network time protocol(NTP)and precision time protocol(PTP)were *** framework for time synchronization of the shore station,undersea junction box layer,and submarine science instrument layer was *** and PTP network signals were decoded by a PTP master clock on a shore station that receives signals from the Global Positioning System and the BeiDou Navigation Satellite System as reference time *** signals were remotely transmitted by a subsea optical–electrical composite cable through an Ethernet passive optical *** time was determined by time synchronization devices in each *** monitoring experiments performed within a laboratory environment indicated that the proposed system is valid and has the potential to realize microsecond accuracy to satisfy the time synchronization requirements of a high-precision seafloor observatory network.

关键词： Seafloor observatory network Junction box IEEE 1588 Time synchronization

Microstructural characterization and oxidation resistance of multicomponent equiatomic CoCrCuFeNi–TiO high-entropy alloy

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International Journal of Minerals,Metallurgy and Materials 2018年第11期25卷 1286-1293页

作者： Qing-dong Qin jin-bo Qu Yong-e Hu Yu-jiao Wu Xiang-dong Su Key Laboratory of Light Metal Materials Processing Technology of Guizhou Province Guizhou Institute of TechnologyGuiyang 550003China Department of Materials&Metallurgy Engineering Guizhou Institute of TechnologyGuiyang 550003China Merchant Marine college Shanghai Maritime UniversityShanghai 201306China 2011 Special Functional Materials Collaborative Innovation Center of Guizhou Province Guiyang 550003China

CoCrCuFeNi–TiO was prepared by arc melting of the pure elements and Ti_2CO powder under an Ar atmosphere. both CoCrCuFe Ni and CoCrCuFeNi–TiO alloys are composed of a face-centered cubic(fcc) solid solution, whereas the alloys of CoCrCuFeNi–TiO are basically composed of an fcc solid solution and TiO crystals. The microstructures of CoCrCuFeNi–TiO are identified as dendrite and interdendrite structures such as CoCrCuFeNi. The morphology of TiO is identified as an equiaxed crystal with a small amount of added Ti_2CO. By increasing the amount of Ti_2CO added, the TiO content was dramatically increased and part of the equiaxed crystals changed to a dendrite structure. A test of the oxidation resistance demonstrates that the oxidation resistance of CoCrCuFeNi–TiO is better than that of CoCrCu Fe Ni. However, as the TiO content increases further, a corresponding decrease is observed in the oxidation resistance.

关键词： multicomponent equiatomic high-entropy alloys microstructure oxidation resistance