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Clinical, cellular, microscopic, and ultrastructural studies of a case of fibrogenesis imperfecta ossium

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Bone Research 2017年第2期5卷 123-136页

作者： Melissa L Barron Mark S Rybchyn Sutharshani Ramesh Rebecca S Mason S Fiona Bonar Paul Stalley Sundeep Khosla Bernie Hudson Christopher Arthur Edward Kim Roderick J Clifton-bligh Phillip B Clifton-bligh department of Physiology School of Medical Sciences Bosch Institute University of Sydney Sydney 2006 New South Wales Australia douglas HanlyMoir Pathology Macquarie Park 2113 New South Wales Australia department of Orthopaedics Royal Prince Alfred HospitalCamperdown 2050 New South Wales Australia department of Endocrinology Mayo Clinic Rochester 55905 MN USA department of Microbiology Royal North Shore Hospital St Leonards 2065 New South Wales Australia department ofMicrobiology Royal North Shore Hospital St Leonards 2065 New South Wales Australia department of Haematology Royal North Shore Hospital St Leonards 2065 New South Wales Australia department of Endocrinology Royal North Shore Hospital St Leonards 2065 New SouthWales Australia and Faculty of Medicine University of Sydney Sydney 2006 New South Wales Australia

Fibrogenesis imperfecta ossium is a rare disorder of bone usually characterized by marked osteopenia and associated with variable osteoporosis and osteosclerosis, changing over time. Histological examination shows that newly formed collagen is abnormal, lacking birefringence when examined by polarized light. The case presented demonstrates these features and, in addition, a previously undocumented finding of a persistent marked reduction of the serum C3 and C4. Osteoblasts established in culture from a bone biopsy showed abnormal morphology on electron microscopy and increased proliferation when cultured with benzoylbenzoyl-ATP and 1,25-dihydroxyvitamin d, contrasting with findings in normal osteoblasts in culture. A gene microarray study showed marked upregulation of the messenger RNA(mRNA) for G-protein-coupled receptor 128(GPR 128), an orphan receptor of unknown function and also of osteoprotegerin in the patient's osteoblasts in culture. When normal osteoblasts were cultured with the patient's serum, there was marked upregulation of the mRNA for aquaporin 1. A single pathogenetic factor to account for the features of this disorder has not been defined, but the unique findings described here may facilitate more definitive investigation of the abnormal bone cell function.

关键词： ultrastructural cellular Clinical

Characterization of the Lipid Components in desmodesmusand ScenedesmusStrains: Lipid Content, Lipid Classes and Fatty Acid Profile

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American Journal of Plant Sciences 2020年第12期11卷 2103-2121页

作者： Carolina Vieira Viegas Gisel Chenard díaz Yordanka Reyes Cruz Leonardo Brantes Bacellar Mendes GREETEC Laboratory Federal University of Rio de Janeiro (UFRJ) Rio de Janeiro Brazil CENPES/PETROBRAS—Leopoldo Américo Miguez de Mello Research Center Rio de Janeiro Brazil

The lipid composition in terms of the amount of neutral lipids, free fatty acids and polar lipid content is of great importance to make full use possible of this fraction and to define the suitability of its application, either as a raw material for fuel production, nutraceutical purposes or feed. In addition to the fatty components present in the lipid extract, other components may be included, such as: carotenoids, pigments and sterols. The microalgae studied in this work, Scenedesmus sp. and Desmodesmus sp., were subjected to the same growth conditions and evaluated for lipid content, quantification and diversity of lipid components as well as its fatty acid profile. For lipid determination two extraction methods were compared: the J: Schmid-Bondzynski-Ratzlaff and Bligh & Dyer method. For Desmodesmus sp. 5.43% ± 0.41% and 9.18% ± 0.33% of lipids were obtained on an ash-free dry weight basis and for Scenedesmus sp. 12.46% ± 0.38% and 8.16% ± 0.42% of lipids were obtained on an ash-free dry weight basis using for both methods J: dana;">Schmid-Bon

关键词： Microalgae desmodesmus sp. Scenedesmus sp.

Quantification of Lipid Content and Identification of the Main Lipid Classes Present in Microalgae Extracts Scenedesmus sp. for Obtaining Fatty Compounds for Biofuel Production

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Energy and Power Engineering 2020年第6期12卷 273-287页

作者： Carolina Vieira Viegas Leonardo Brantes Bacellar Mendes Rafael Richard João Gisel Chenard díaz donato A. G. Aranda Yordanka Reyes Cruz GREETEC Laboratory Federal University of Rio de Janeiro (UFRJ) Rio de Janeiro Brazil CENPES/PETROBRáS—Leopoldo Américo Miguez de Mello Research Center Rio de Janeiro Brazil

Microalgae biomass has been reported in the literature as one of the most promising sources for obtaining different products of industrial interest such as lipids, fatty acids, carotenoids, proteins and fibers. The lipid fraction of microalgae comprises neutral lipids, free fatty acids and polar lipids. It is of great importance to estimate the composition of the lipid fraction to define the potential for use, either as a raw material for the production of biofuels or for use for nutraceuticals and/or food purposes. The microalgae Scenedesmus sp. cultivated in a photobioreactor, the sky open raceway type, was evaluated for lipid content, identification and quantification of lipid components obtained from different extracts. In the quantification of the lipid content, extraction methods were proposed without chemical treatment (use of solvents only) such as chloroform:methanol (2:1 v/v)—bligh & dyer, Ethanol, Ethyl acetate:Hexane (1:1 v/v) and others with chemical treatment such as J-Schmid-Bondzynski-Ratzlaff (acid) and saponification (basic). For the identification of the main lipid components present in the extracts, the Thin layer chromatography (TLC) technique was used. This made it possible, using a simple and inexpens

关键词： Microalgae Lipids Extraction Saponifiable Compounds Biofuels

Cultivation of Microalgae Monoraphidiumsp., in the Plant Pilot the Grand Valle Bio Energy, for Biodiesel Production

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Natural Science 2015年第7期7卷 370-378页

作者： Gisel Chenard díaz Yordanka Reyes Cruz René González Carliz Rosa C. Vitorino de Paula donato A. Gomes Aranda Marcellus A. G. dario Gustavo Saraiva Marass Nelson C. Furtado Grand Valle Bio Energy Ltda. Rio de Janeiro Brazil Greentec Laboratory School of Chemistry Federal University of Rio de Janeiro Rio de Janeiro Brazil Brazilian Center for Physical Researches Rio de Janeiro Brazil

At present, Brazil imports approximately 11 billion liters/year of diesel. With the interruption of the works in the new Petrobras refineries, the projection is that by 2025 this volume will increase to 24.2 billion liters of diesel/year. In this sense, the biodiesel factory Grand Valle Bio Energy Ltda., located in the state of Rio de Janeiro, in conjunction with the FAPERJ makes some investments in technology development for the cultivation and use of microalgae as an alternative raw material in the production of biodiesel. Based on arguments previously said, this work presents the results of the microalgae cultivation Monoraphidium sp. in photobioreactors the pilot plant of the company. The installation with an area of 120 m2 is included with 2 open photobioreactors of type falling film (20 m × 1 m), with a cascade of 18mm and capacity of 4000 L. The lineage cultivated is selected from previous ecophysiological studies that are identified as promising for biodiesel production by having a high potential for the production of lipids. This lineage is maintained at collection of the stock of cultures Laboratory of Green Technologies of the School of Chemistry/ UFRJ. The cultivation was performed in means ASM-1 (Gorham et al., 1964), initial pH 8.0, with aeration and circulation average of 8 hours a day during 19 days. The culture was started with an inoculum of 1 × 107 cel/ml. The lipid production was determined in two phases of growth: on day 4 (exponential phase) and 15 day (stationary phase). For the determination and quantification of lipid content, two different methods were assessed for a sample of biomass, submitted to the same processes the separation and drying. The results showed the methodology of bligh & dyer with modifications as the most efficient in extracting lipids. The total lipid content of the biomass Monoraphidium sp. was 30.58%. The growth rate varied between 0.74 ± 0.01 and 0.68 ± 0.02.

关键词： Cultivation Photobioreactors Microalgae Biodiesel