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Characterisation of the Bacteria and Archaea Community Associated with Wild Oysters, At Three Possible Restoration Sites in the North Sea

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Open Journal of Marine Science 2024年第2期14卷 19-40页

作者： Natacha M. S. Juste-Poinapen Yang Lu bla.ca.bola.os de hoyos George C. Birch camille Saurel Oyster Heaven Rotterdam Netherlands Nucleus Rotterdam Netherlands Microbial Ecology and Wastewater Microbiology Queensland University of Technology Brisbane Australia National Institute of Aquatic Resources Technical University of Denmark Lyngby Denmark

With 85% of the global oyster reefs destroyed, there is an urgent need for large scale restoration to benefit from the ecosystem services provided by biogenic oyster reefs and their associated biodiversity, including microorganisms that drive marine biogeochemical cycles. This experiment established a baseline for the monitoring of the bacterial and archaeal community associated with wild oysters, using samples from their immediate environment of the Voordelta, with cohabiting Crassostrea gigas and ostrea edulis, Duikplaats with only C. gigas attached to rocks, and the Dansk Skaldyrcentre, with no onsite oysters. The microbial profiling was carried out through DNA analysis of samples collected from the surfaces of oyster shells and their substrate, the sediment and seawater. Following 16S rRNA amplicon sequencing and bioinformatics, alpha indices implied high species abundance and diversity in sediment but low abundance in seawater. As expected, Proteobacteria, Bacteroidetes, Firmicutes and Thaumarchaeota dominated the top 20 OTUs. In the Voordelta, OTUs related to Colwellia, Shewanella and Psychrobium differentiated the oysters collected from a reef with those attached to rocks. Duikplaats were distinct for sulfur-oxidizers Sulfurimonas and sulfate-reducers from the Sva 0081 sediment group. Archaea were found mainly in sediments and the oyster associated microbiome, with greater abundance at the reef site, consisting mostly of Thaumarchaeota from the family Nitrosopumilaceae. The oyster free site displayed archaea in sediments only, and algal bloom indicator microorganisms from the Rhodobacteraceae, Flavobacteriaceae family and genus [Polaribacter] huanghezhanensis, in addition to the ascidian symbiotic partner, Synechococcus. This study suggests site specific microbiome shifts, influenced by the presence of oysters and the type of substrate.

关键词： Oyster Reefs Microbiome Marine Bacteria Marine Archaea Restoration

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Growing arugula plants using aeroponic culture with an automated irrigation system

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International Journal of Agricultural and Biological Engineering 2020年第3期13卷 52-56页

作者： Jhon D.Ríos Salazar John E.candelo-Becerra Fredy E.hoyos Velasco Escuela de Física Facultad de CienciasUniversidad Nacional de ColombiaSede MedellínColombia departamento de Energía Eléctrica y Automática Facultad de MinasUniversidad Nacional de ColombiaSede MedellínColombia

The paper presents an efficient form of growing arugula plants by means of automatic control of an aeroponic culture irrigation *** system considers a reprogrammable electronic circuit that uses software to generate different irrigation cycles to obtain an adequate growth of arugula *** show how different samples grown in a greenhouse had the same growth behavior as field-grown samples during the test *** was possible to obtain a more efficient and sustained five-week production to supply consumers by having a continuous cycle irrigation system,which was operated for 35 *** growth and number of leaves were maintained in a similar way for different plants *** grow similarly,but some of them showed size differences during the five weeks.

关键词： aeroponic culture arugula plants food production irrigation systems smart system

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Function of hepatocyte spheroids in bioactive microcapsules is enhanced by endogenous and exogenous hepatocyte growth factor

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Bioactive Materials 2023年第10期28卷 183-195页

作者： Kihak Gwon Daheui Choi Jose Mde hoyos-Vega Harihara Baskaran Alan M.Gonzalez-Suarez Seonhwa Lee Hye Jin Hong Kianna M.Nguyen Ether Dharmesh Go Sugahara Yuji Ishida Takeshi Saito Gulnaz Stybayeva Alexander Revzin department of Physiology and Biomedical Engineering Mayo ClinicRochesterMNUSA department of Chemical and Biomolecular Engineering Case Western Reserve UniversityClevelandOHUSA Biomedical Engineering Saint Louis UniversitySt.LouisMOUSA University of Southern californi1 Keck School of MedicineDepartment of MedicineDivision of Gastrointestinal and Liver DiseasesLos AngelesCAUSA Research and development department PhoenixBioCo.LtdKagamiyam1Higashi-Hiroshim1Hiroshim1Japan USC Research Center for Liver Diseases Los AngelesCAUSA

The ability to maintain functional hepatocytes has important implications for bioartificial liver development,cell-based therapies,drug screening,and tissue *** approaches can be used to restore hepatocyte function in vitro,including coating a culture substrate with extracellular matrix(ECM),encapsulating cells within biomimetic gels(Collagen-or Matrigel-based),or co-cultivation with other *** paper describes the use of bioactive heparin-based core-shell microcapsules to form and cultivate hepatocyte *** microcapsules are comprised of an aqueous core that facilitates hepatocyte aggregation into spheroids and a heparin hydrogel shell that binds and releases growth *** demonstrate that bioactive microcapsules retain and release endogenous signals thus enhancing the function of encapsulated *** also demonstrate that hepatic function may be further enhanced by loading exogenous hepatocyte growth factor(HGF)into microcapsules and inhibiting transforming growth factor(TGF)-β1 ***,bioactive microcapsules described here represent a promising new strategy for the encapsulation and maintenance of primary hepatocytes and will be beneficial for liver tissue engineering,regenerative medicine,and drug testing applications.

关键词： Droplet microfluidics Bioactive microcapsules Hepatocyte spheroid Heparin Hepatocyte growth factor

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利用可自动灌溉的雾化栽培系统种植芝麻作物

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农业工程技术 2020年第33期40卷 88-88页

作者： Jhon D.Ríos Salazar John E.candelo-Becerra Fredy E.hoyos Velasco 国立哥伦比亚大学科学学院物理系国立哥伦比亚大学电能自动化系

介绍了一种利用可灌溉雾化栽培系统自动控制种植芝麻作物的有效方式。该系统采用一个可重新编程的电子电路,使用软件生成不同的灌溉周期,以获得芝麻作物的充分生长。结果表明,在试验期间,温室中生长的样品与田间生长的样品具有相同的生... 详细信息

介绍了一种利用可灌溉雾化栽培系统自动控制种植芝麻作物的有效方式。该系统采用一个可重新编程的电子电路,使用软件生成不同的灌溉周期,以获得芝麻作物的充分生长。结果表明,在试验期间,温室中生长的样品与田间生长的样品具有相同的生长行为。通过连续循环灌溉35天,可以获得更高效、更持久的五周产量,以供应消费者。所分析的不同植物的叶片生长和数量均保持一致。根系生长状况相似,只是其中一些在五周内表现出大小差异。

关键词：雾化栽培芝麻作物粮食生产灌溉系统智能系统

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