检索结果-南通市图书馆

cDNA cloning,pituitary location and extra-pituitary expression of pro-opiodmelanocortin（POMC） gene in Rare Gudgeon（Gobiocypris rarus）

在线全文

学校读者我要写书评

暂无评论

cDNA cloning,pituitary location and extra-pituitary expressi...

Twelve novel microsatellite markers for the Chinese golden loach Sinibotia superciliaris

中国鱼类学会2008年学术研讨会

作者： Xiaohong Liu,biwen xie,Yaoguang Zhang,Zhijian Wang 西南大学生命科学学院

＜正＞A cDNA encoding pro-opiodmelanocortin（POMC） was cloned form pituitary of the Rare gudgeon（Gobiocypris rarus）,a Chinese suvivaling small fresh water fish,and its pituitary and tissue specific expression were ...

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Twelve novel microsatellite markers for the Chinese golden l...

Induction and identification of artificial triploid in snakehead(Channa argus)

第十三届全国野生动物生态与资源保护学术研讨会暨第六届中国西部动物学学术研讨会

作者： Shiying Liu Yongming Wang biwen xie Zuogang Peng Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education) Life Sciences Conservation and Utilization of Fish Resources in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province Neijiang Normal University College of Life Sciences

The Chinese golden loach,Sinibotia superciliaris(Teleostei,Cypriniformes,Botiidae),is a tetraploid fish species and endemic to the middle and upper reaches of the Yangtze River in *** to its high possibility of vulner...

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Induction and identification of artificial triploid in snake...

Status of aquatic organisms resources and their environments in Yangtze River system(2017-2021)⋆

四川省动物学会第十届会员代表大会暨第十一次学术研讨会

作者： Yuanchao ZOU Zhong LI Zhengyong WEN Chuan Jie QIN biwen xie Xingjian YUE Yu WANG Zemin QI College of Life Sciences Neijiang Normal UniversityKey Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River Key Laboratory of Freshwater Fish Reproduction and Development Ministry of EducationSchool of Life ScienceSouthwest University

snakehead(Channa argus) is a commercially important cultured fresh fish in *** induced triploidy resulting in genetic ***,triploidy induction can control unwanted reproduction in culture and can be an advantage in those species with high mortality during or after the spawning *** this,the development of all-triploid stock would be of significant benefit for *** the present study,a set of experiments were carried out for developing artificial all-triploids offspring in the *** heat shock to inhibit the second polar body extrusion of fertilized egg and the optimum conditions for retention of the second polar body in *** were investigated by altering initiation time,temperature and duration of heat shock *** the incubation temperature is about 28 ℃±0.5 ℃,after fertilization 4 min,use 42 ℃ heat shock treatment temperature for 4 min,highest triploid induction rate of up to 87.69%.Ploidy of fry was analyzed using flow *** triploid cellular DNA contents of *** was 1.50 times to the diploid’*** red blood cell size identification method to identify the *** the erythrocyte volume,nucleus volume there is a significant difference(P﹤0.01).The erythrocyte volume and nucleus volume of triploid are 1.69 and 1.60 times to the diploid,*** technique lays foundation and provides important tool for sex control and production of high quality seeds in ***.

关键词： snakehead(Channa argus) triploid induction heat shock

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Aquaculture and Fisheries 2024年第5期9卷 833-850页

作者： Haile Yang Li Shen Yongfeng He Huiwu Tian Lei Gao Jinming Wu Zhigang Mei Nian Wei Lin Wang Tingbing Zhu Feifei Hu Jinling Gong Hongchun Du Xinbin Duan Huatang Deng Daoqun Wang Fengyue Zhu Yunfeng Li Fan Wu Huijun Ru Yan Zhang Junyi Li Junlin Yang Yuntao Zhou Dongdong Fang Yinping Wang Danqing Lin Yanping Yang Peijie Li Silei Liu Jian Yang Ping Zhuang Sikai Wang Tao Zhang Gang Yang Wenbo Yang Lilai Yuan Kun Cao Shuo Xu Huiyuan Liu Zhiqiang Liang Chongrui Wang Hong Li Xiping Yuan Xin Yang Yilong Fu Yanping Zhang Haixin Zhang Zhiying Tao Sheng Wang Xiaoping Gao Binsong Jin Kemao Li Guojie Wang Shenglong Jian Yingqin Li Chenjiang Xue Chunyun Lei Shaowei Xue Yi Sun Bin Zhu Ke Shao Xingkun Hu Meihua Xiong Jun Du Bin He Tao Yan Yingying Huang Yuanchao Zou biwen xie Yongming Wang Bin Li Fei Liu Yaoyao Zhang Fei Fan Zhijian Wang Jing Huang Haoran Gu Hailong Ge Yan Dan Yan Li Shuqiao Wang Chuang Zhang Lu Zhou Xue Wang Sheng Zeng Yan Xiang Xugang He Jianhui Qin Chengxing Xia Jie Hou Yifu Shi Lifang Gao Zhiqiang Zhu Hongbao Shen Yun Du Xuejun Duan Jiawu Xiong Deguo Yang Shaoping Liu Zhaohui Ni Hui Zhang Kai Liu Feng Zhao Yingren Li Jianwei Wang Qiwei Wei Yangtze River Fisheries Research Institute Chinese Academy of Fishery SciencesWuhan430223China Institute of Hydrobiology Chinese Academy of SciencesWuhan430072China Fishery Resource and Environment Research Center Chinese Academy of Fishery SciencesBeijing100141China Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization Ministry of Agriculture and Rural AffairsFreshwater Fisheries Research CenterChinese Academy of Fishery SciencesWuxi214081China East China Sea Fisheries Research Institute Chinese Academy of Fishery SciencesShanghai200090China Institute of Fisheries Engineering Chinese Academy of Fishery SciencesBeijing100141China Hunan Fisheries Science Institute Changsha410153China Jiangxi Fisheries Research Institute Nanchang330039China Jiujiang Academy of Fishery Sciences Jiujiang332000China School of Life Sciences Nanchang UniversityNanchang330031China Qinghai Provincial Fishery Technology Extension Center Xining810012China Yunnan Academy of Fishery Sciences Kunming650111China Institute of Hydroecology Ministry of Water Resources and Chinese Academy of SciencesWuhan430079China Fisheries Institute Sichuan Academy of Agricultural SciencesChengdu611731China School of Life Sciences Neijiang Normal UniversityNeijiang641100China School of Life Sciences Southwest UniversityChongqing400715China Chongqing Fishery Sciences Research Institute Chongqing401120China Guizhou Fisheries Research Institute Guiyang550025China College of Fisheries Huazhong Agricultural UniversityWuhan430072China Hubei Fisheries Science Research Institute Wuhan430070China Shaanxi Fisheries Research Institute Xi’an710086China Innovation Academy for Precision Measurement Science and Technology Chinese Academy of SciencesWuhan430077China Nanjing Institute of Geography and Limnology Chinese Academy of SciencesNanjing210008China Central South Inventory and Planning Institute of State Forestry and Grassland Administration Changsha410014China

The Yangtze River is the mother river of *** promote the aquatic ecosystem protection of the great river,the Project of Yangtze Fisheries Resources and Environment Investigation(2017-2021)supported by the Ministry of Agriculture and Rural Affairs,*** carried out by 24 institutes and universities that located in the Yangtze River basin surveys the status of(1)fish species composition and spatial distribution,(2)current fish abundance,(3)endangered fishes,(4)Yangtze finless porpoise,(5)aquatic eco-environments,(6)water-level fluctuation areas,(7)capture fisheries and recreational fisheries of the Yangtze River mainstream and 10 of its main tributaries,including Yalong River,Heng River,Min River(including Dadu River),Chishui River,Tuo River,Jialing River,Wu River,Han River,Dongting Lake and Poyang *** results showed that there were 443 fish species(belonging to 163 genus,37 families,and 18 orders)before 2017,but only 323 fish species(including 15 new recorded exotic species)were recorded in the project of *** them,Cyprinus carpio,Carassius auratus,Hypophthalmichthys molitrix,Pelteobagrus fulvidraco,Coilia brachygnathus,Silurus asotus,Saurogobio dabryi,Ctenopharyngodon idellus,Pelteobagrus nitidus,Hemiculter leucisculus,Siniperca chuatsi,Coreius heterodon,Culter alburnus,Parabramis pekinensis,and Aristichthys nobilis were the dominant species across the whole Yangtze River *** is estimated that there were 886 million individuals weighing 124.8 million kg,merely equivalent to 27.3%of the resources in 1950s,30.9%of the resources in 1960s,or 58.7%of the resources in *** the new list of protected fishes that recorded in the Yangtze River system,only 15 of 29 were collected in this project of *** gladius has been affirmed to be Extinct by International Union for Conservation of Nature(IUCN).The wild individuals of Tenualosa reevesii and Luciobrama macrocephalus have disappeared for many years and maybe have been extinct

关键词： Fishes Yangtze finless porpoise Resources Aquatic ecosystem Fisheries Background investigation Yangtze river system

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

5种经济鱼类基因组大小的测定

华中农业大学学报 2018年第3期37卷 87-91页

作者：邹远超符鹏李骞吴田王永明谢碧文长江上游鱼类资源保护与利用四川省重点实验室/内江师范学院生命科学学院内江641112

采用流式细胞术,以鸡(Gallus gallus)血细胞DNA含量(2.3pg/2C,C指单倍体)为标准,对5种经济鱼类白甲鱼(Onychostoma sima)、三倍体兴国红鲤(triploid Cyprinus carpio ***)、胭脂鱼(Myxocyprinus asiaticus)、中华沙鳅(Sinibotia superciliaris)和花鱼骨(Hemibarbus maculatus)的基因组大小(或称C-值)和倍性进行测定。结果表明,5种淡水鱼类的的C-值分别为:白甲鱼(1.18±0.01)pg,三倍体兴国红鲤(1.92±0.07)pg,胭脂鱼(2.62±0.28)pg,中华沙鳅(0.89±0.01)pg,花鱼骨(1.24±0.02)pg,C-值从大到小排列依次为:胭脂鱼>兴国红鲤>花鱼骨>白甲鱼>中华沙鳅。在5种经济鱼类中,除了亲缘关系很近的白甲鱼与花鱼骨(同一科)的C-值大小差异不显著外(P>0.05),其余的种间(不同科)的C-值大小差异显著。倍性分析结果表明,白甲鱼、花鱼骨和中华沙鳅属于二倍体,兴国红鲤属于三倍体,胭脂鱼属于四倍体。

关键词：基因组大小 DNA含量流式细胞术

在线全文

学校读者我要写书评

暂无评论

地方本科院校养殖水化学实践教学改革的探索

实验室研究与探索 2018年第5期37卷 245-247,265页

作者：陶敏谢碧文文正勇王永明李斌内江师范学院生命科学学院四川内江641112

人才培养目标和学生实际情况是进行教学改革必须兼顾的两个方面。以内江师范学院为例,从专业实践教学体系的角度出发,依据培养实用技能型水产人才的目标以及职业高中生源的学情,探讨了地方本科院校养殖水化学实践教学改革的途径。实践表... 详细信息

人才培养目标和学生实际情况是进行教学改革必须兼顾的两个方面。以内江师范学院为例,从专业实践教学体系的角度出发,依据培养实用技能型水产人才的目标以及职业高中生源的学情,探讨了地方本科院校养殖水化学实践教学改革的途径。实践表明,合理的行课时间、课时分配、实践内容和教学方式能有效激发学生的积极性,提高学生操作技能的熟练程度,还能培养学生独立思考的能力与深厚的专业情感。

关键词：地方本科院校养殖水化学实践教学课程实习职业高中生源

在线全文

学校读者我要写书评

暂无评论

切尾拟鲿外周血细胞的显微结构

四川动物 2014年第2期33卷 234-238页

作者：邹远超岳静岳兴建王永明谢碧文齐泽民内江师范学院生命科学学院长江上游鱼类资源保护与利用四川省重点实验室四川内江641000

应用Wright-Giemsa染液联合染色方法,通过光镜对切尾拟鲿Pseudobagrus truncatus外周血细胞的形态进行观察。结果表明,切尾拟鲿外周血细胞可分为红细胞、单核细胞、淋巴细胞、血栓细胞和嗜中性粒细胞、嗜酸性粒细胞两种类型的粒细胞。... 详细信息

应用Wright-Giemsa染液联合染色方法,通过光镜对切尾拟鲿Pseudobagrus truncatus外周血细胞的形态进行观察。结果表明,切尾拟鲿外周血细胞可分为红细胞、单核细胞、淋巴细胞、血栓细胞和嗜中性粒细胞、嗜酸性粒细胞两种类型的粒细胞。未发现嗜碱性粒细胞。白细胞中,血栓细胞数量最多,占白细胞总数的39.32%;嗜酸性粒细胞数量最少,仅占白细胞总数的2.30%;单核细胞体积最大,大小为(14.58±3.95)μm×(12.96±2.67)μm;血栓细胞体积最小,大小为(6.53±0.96)μm×(3.65±0.41)μm。此外,统计了红细胞密度为(1.68±0.39)×106/mm3,白细胞密度为(5.84±0.73)×103/mm3。

关键词：切尾拟鲿血细胞显微结构

中华沙鳅(♀)与宽体沙鳅(♂)杂交种的胚胎发育

在线全文

学校读者我要写书评

暂无评论

四川动物 2014年第1期33卷 90-93,98页

作者：王永明谢碧文岳兴建王芳齐泽民王淯吴英长江上游鱼类资源保护与利用四川省重点实验室、特色农业资源研究与利用四川省高校重点实验室四川内江641112 内江市第六中学四川内江641000

以中华沙鳅为母本,宽体沙鳅为父本,通过人工杂交获得受精卵,运用数码体视显微镜对杂交种的胚胎发育进行观察拍照,并对杂交种胚胎发育各时期特征进行了详细描述。受精卵直径1.41 mm±0.16 mm,吸水膨胀后直径达1.91 mm±0.09 mm,半漂流性... 详细信息

以中华沙鳅为母本,宽体沙鳅为父本,通过人工杂交获得受精卵,运用数码体视显微镜对杂交种的胚胎发育进行观察拍照,并对杂交种胚胎发育各时期特征进行了详细描述。受精卵直径1.41 mm±0.16 mm,吸水膨胀后直径达1.91 mm±0.09 mm,半漂流性。25℃±0.5℃水温条件下,受精卵历时25 h 50 min孵出。胚胎发育过程分为7个阶段、27个发育期。初孵仔鱼全长4.71 mm±0.12 mm。经统计,杂交种受精率46.84%,孵化率72.84%,仔鱼畸形率8.62%,表明这两种沙鳅属间杂交表现出亲和性。

关键词：杂交种胚胎发育中华沙鳅宽体沙鳅

在线全文

学校读者我要写书评

暂无评论

黄石爬鮡消化系统组织学观察

重庆师范大学学报（自然科学版） 2015年第6期32卷 42-45,F0002页

作者：王永明申绍祎史晋绒谢碧文齐泽民王芳内江师范学院生命科学学院长江上游鱼类资源保护与利用四川省重点实验室四川内江641112

采用解剖和光镜技术详细观察了黄石爬鮡(Euchiloglanis kishinouyei)消化系统的组织结构。结果显示黄石爬鮡的消化系统由消化道和消化腺组成:1)消化道包括口咽腔、食道、胃、肠及肛门共5部分组成,肠道系数(肠道长度与鱼体全长的比值)为0... 详细信息

采用解剖和光镜技术详细观察了黄石爬鮡(Euchiloglanis kishinouyei)消化系统的组织结构。结果显示黄石爬鮡的消化系统由消化道和消化腺组成:1)消化道包括口咽腔、食道、胃、肠及肛门共5部分组成,肠道系数(肠道长度与鱼体全长的比值)为0.52±0.05,为典型的肉食性鱼类;口咽腔及食道粘膜层上皮为复层扁平上皮,内含杯状细胞、棒状细胞及味蕾;胃呈"V"形,粘膜层上皮为单层柱状上皮,无杯状细胞,胃腺在贲门部及盲囊部丰富,幽门部缺失;肠由前肠、中肠、后肠等3部分组成,肠道由前向后粘膜皱褶数量依次减少,粘膜皱褶及粘膜上皮柱状细胞高度依次降低,杯状细胞数量逐渐增多,肌肉层逐渐增厚。2)消化腺由肝胰脏和胆囊组成,肝脏分两叶,胰腺弥散分布于肝脏和肠系膜上,胆囊呈椭圆形,体积较大。黄石爬鮡消化系统的组织结构特点与其消化、吸收作用密切相关。

关键词：黄石爬鮡消化系统组织学