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Increasingγ-CD conversion rates by improving thermostability of Bacillus ***-44876γ-CGTase

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Food Bioscience 2023年第1期51卷 346-354页

作者： Xiaoxiao Li Danni Zheng Jing Wu Zhengyu Jin birte svensson Yuxiang Bai State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxiJiangsu214122China School of Food Science and Technology Jiangnan UniversityWuxiJiangsu214122China Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province Jiangnan UniversityWuxiJiangsu214122China Enzyme and Protein Chemistry Department of Biotechnology and BiomedicineTechnical University of DenmarkDK-2800 KgsLyngbyDenmark

Poor thermostability is a limiting factor for applications ofγ-CGTase that affects starch utilization and yield ofγ-*** thermostability of Bacillus ***-44876γ-CGTase(BFγ-CGTase)was improved by addition of Ca^(2+)and site-directed ***,10 mM Ca^(2+)increased the half-life(t1/2)at 55 and 60℃ from 3.0 to 0.3 h to 17.4 and 2.0 h,*** spectra indicated that Ca^(2+)stabilized the tertiary structure of the BFγ-CGTase and hence improved the *** to serine of a glycine residue in anα-helix related to thermostability also improved the stability especially at the higher ***,in 10 mM Ca^(2+)the G208S mutant further increased t1/2 to 20.0 h and 4.0 h at 55 and 60℃,*** G208S mutant in 10 mM Ca^(2+)producedγ-CD from tapioca starch with 40%increased yield of that from BFγ-*** work involving rational protein engineering provided a new tool for enzymaticγ-CD production.

关键词： Starch Protein engineering Three-dimensional structure

Atligator Web:A Graphical User Interface for Analysis and Design of Protein-Peptide Interactions

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BioDesign Research 2023年第1期5卷 80-85页

作者： Josef Paul Kynast birte Höcker Department of Biochemistry University of BayreuthBayreuthGermany.

A key functionality of proteins is based on their ability to form interactions with other proteins or *** interactions are neither easily described nor fully understood,which is why the design of specific protein–protein interaction interfaces remains a challenge for protein *** recently developed the software ATLIGATOR to extract common interaction patterns between different types of amino acids and store them in a *** tool enables the user to better understand frequent interaction patterns and find groups of ***,frequent motifs can be directly transferred from the database to a user-defined scaffold as a starting point for the engineering of new binding *** three-dimensional visualization is a crucial part of ATLIGATOR,we created ATLIGATOR web—a web server offering an intuitive graphical user interface(GUI)available at https://*** new interface empowers users to apply ATLIGATOR by providing easy access with having all parts directly ***,we extended the web by a design functionality so that,overall,ATLIGATOR web facilitates the use of ATLIGATOR with a more intuitive UI and advanced design options.

关键词： Web server intuitive

Coding variants of the interleukin-11 receptor with reduced protein maturation show protease-dependent trans-signaling and transduce normal STAT3 signaling

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Genes & Diseases 2023年第2期10卷 373-376页

作者： birte Kespohl Roland Hartig Yvonne Garbers Juliane Lokau Christoph Garbers Department of Pathology Otto-von-Guericke-UniversityMedical Faculty39120 MagdeburgGermany Health Campus Immunology Infectiology and InflammationOtto-von-Guericke-University39120 MagdeburgGermany Center for Health and Medical Prevention (ChaMP) Otto-von-Guericke-University39120 MagdeburgGermany Institute for Molecular and Clinical Immunology and Service Unit Multiparametric Bioimaging and Cytometry Medical FacultyUniversity of Magdeburg39120 MagdeburgGermany Institute of Communication Management Faculty of ManagementCulture and Technology (Lingen Campus)Osnabrück University of Applied Sciences49809 LingenGermany

Interleukin-11(IL-11)is a member of the IL-6 family of cytokines.1 IL-11 activates its target cells via binding to the IL-11 receptor(IL-11R),which also exists in soluble forms generated by proteolytic cleavage(sIL-11R,required for trans-signaling).2 Formation of the IL-11/IL-11R complex leads to the recruitment and homodimerization of two molecules of the signal-transducingβ-receptor gp130,which then activates intracellular signaling cascades,including the Jak/STAT,PI3K and ERK pathways.

关键词： STAT3 PI3K gp130

Interventional strategies in infected necrotizing pancreatitis:Indications,timing,and outcomes

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World Journal of Gastroenterology 2022年第27期28卷 3383-3397页

作者： birte Purschke Louisa Bolm Max Nikolaus Meyer Hiroki Sato Department of Gastrointestinal Surgery Massachusetts General HospitalBostonMA 02114United States Department of Radiation Oncology Massachusetts General HospitalBostonMA 02114United States Department of Medicine Asahikawa Medical UniversityAsahikawa 0788510HokkaidoJapan Department of Gastroenterology Asahikawa Kosei HospitalAsahikawa 0788211HokkaidoJapan

Acute pancreatitis(AP)is one of the most common gastrointestinal diseases and remains a life-threatening *** AP resolves to restitutio ad integrum in approximately 80%of patients,it can progress to necrotizing pancreatitis(NP).NP is associated with superinfection in a third of patients,leading to an increase in mortality rate of up to 40%.Accurate and early diagnosis of NP and associated complications,as well as state-of-the-art therapy are essential to improve patient *** emerging role of endoscopy and recent trials on multidisciplinary management of NP established the“step-up approach”.This approach starts with endoscopic interventions and can be escalated to other interventional and ultimately surgical procedures if *** showed that this approach decreases the incidence of new multiple-organ failure as well as the risk of interventional ***,the optimal interventional sequence and timing of interventional procedures remain *** review aims to summarize the indications,timing,and treatment outcomes for infected NP and to provide guidance on multidisciplinary decision-making.

关键词： Pancreatitis Acute necrotizing pancreatitis Necrosis Superinfection Endoscopy Surgery

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一所上海小学给我的印象

上海教育 2002年第7期 50-51页

作者： birte Seffert 本刊实习生

作为一个来自德国首都柏林的四年级大学生,我来中国的目的不只是把中文学好,而且要了解与研究中国快速发展的教育。在上海我有机会在《上海教育》杂志实习。通过对一些上海学校的采访,我发觉德国与中国教育的一些不同。这篇小文章不是... 详细信息

作为一个来自德国首都柏林的四年级大学生,我来中国的目的不只是把中文学好,而且要了解与研究中国快速发展的教育。在上海我有机会在《上海教育》杂志实习。通过对一些上海学校的采访,我发觉德国与中国教育的一些不同。这篇小文章不是系统地分析两个国家的教育,这种任务超出我的能力。作为《上海教育》杂志的实习生,我访问了一所上海的小学,并将在这

关键词：小学生学生服愚园路

Limited resection for duodenal gastrointestinal stromal tumors:Surgical management and clinical outcome

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World Journal of Gastrointestinal Surgery 2013年第2期5卷 16-21页

作者： Jens Hoeppner birte Kulemann Goran Marjanovic Peter Bronsert Ulrich Theodor Hopt Department of General Surgery University of Freiburg79106 FreiburgGermany Department of Pathology University of Freiburg79106 FreiburgGermany

AIM:To analyze our experience in patients with duodenal gastrointestinal stromal tumors(GIST) and review the appropriate surgical ***:We retrospectively reviewed the medical records of all patients with duodenal GIST surgically treated at our medical institution between 2002 and *** files,operative reports,radiological charts and pathology were *** surgical therapy open and laparoscopic wedge resections and segmental resections were performed for limited resection(LR).For extended resection pancreatoduodenectomy was ***,gender,clinical symptoms of the tumor,anatomical localization,tumor size,mitotic count,type of resection resectional status,neoadjuvant therapy,adjuvant therapy,risk classification and follow-up details were investigated in this retrospective ***:Nine patients(5 males/4 females) with a median age of 58 years were surgically *** median follow-up period was 45 mo(range 6-111 mo).The initial symptom in 6 of 9 patients was gastrointestinal bleeding(67%).Tumors were found in all four parts of the duodenum,but were predominantly located in the first and second part of the duodenum with each 3 of 9 patients(33%).Two patients received neoadjuvant medical treatment with 400 mg imatinib per day for 12 wk before *** one patient,the GIST resection was done by *** 8 LRs included a segmental resection of pars 4 of the duodenum,5 wedge resections with primary closure and a wedge resection with luminal closure by Roux-Y *** of these LRs was done minimally invasive;seven were done in open *** median diameter of the tumors was 54 mm(14-110 mm).Using the Fletcher classification scheme,3/9(33%) tumors had high risk,1/9(11%) had intermediate risk,4/9(44%) had low risk,and 1/9(11%) had very low risk for aggressive *** resections showed microscopically negative transsection margins(R0),two showed positive margins(R1).No patient developed local r

关键词： Gastrointestinal stromal tumor Duodenum Surgery Limited resection Survival

Impact of remote ischemic preconditioning on wound healing in small bowel anastomoses

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World Journal of Gastroenterology 2011年第10期17卷 1308-1316页

作者： Philipp Anton Holzner birte Kulemann Simon Kuesters Sylvia Timme Jens Hoeppner Ulrich Theodor Hopt Goran Marjanovic Surgical Metabolic and Anastomotic Research Team Department of General and Visceral SurgeryAlbert Ludwigs University of Freiburg Institute of Pathology Albert Ludwigs University of Freiburg

AIM： To investigate the influence of remote ischemic preconditioning （RIPC） on anastomotic integrity. METHODS： Sixty male Wistar rats were randomized to six groups. The control group （n = 10） had an end- to-end ileal anastomosis without RIPC. The preconditioned groups （n = 34） varied in time of ischemia and time of reperfusion. One group received the amino acid L-arginine before constructing the anastomosis （n = 9）. On postoperative day 4, the rats were re-laparotomized, and bursting pressure, hydroxyproline concentration, intra-abdominal adhesions, and a histological score concerning the mucosat ischemic injury were collected. The data are given as median （range）.RESULTS： On postoperative day 4, median bursting pressure was 124 mmHg （60-146 mmHg） in the control group. The experimental groups did not show a statistically significant difference （P 〉 0.05）. Regarding the hydroxyproline concentration, we did not find any significant variation in the experimental groups. We detected significantly less mucosal injury in the RIPC groups. Furthermore, we assessed more extensive intra-abdominal adhesions in the preconditioned groups than in the control group. CONCLUSION： RIPC directly before performing small bowel anastomosis does not affect anastomotic stability in the early period, as seen in ischemic preconditioning.

关键词： Anastomotic healing Hydroxyproline Bursting pressure Mucosal injury index Wound healing Remote ischemic preconditioning

Cross-neutralization and cross-protection among SARS-CoV-2 viruses bearing different variant spikes

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Signal Transduction and Targeted Therapy 2022年第9期7卷 3202-3205页

作者： Yang Liu Jianying Liu Jing Zou birte Kalveram Rafael R.G.Machado Ping Ren Sina Tureli Derek J.Smith Scott C.Weaver Xuping Xie Pei-Yong Shi Department of Biochemistry and Molecular Biology University of Texas Medical BranchGalvestonTXUSA Institute of Infectious Diseases Shenzhen Bay LaboratoryShenzhenChina Department of Microbiology and Immunology University of Texas Medical BranchGalvestonTXUSA Department of Pathology University of Texas Medical BranchGalvestonTXUSA Center for Pathogen Evolution Department of ZoologyUniversity of CambridgeCambridgeUK Sealy Institute for Drug Discovery University of Texas Medical BranchGalvestonTXUSA Sealy Center for Structural Biology&Molecular Biophysics University of Texas Medical BranchGalvestonTXUSA

Dear Editor The rapid evolution of SARS-CoV-2 mandates a better under-standing of cross-neutralization and cross-protection among *** information is essential to guide vaccine strategy and public *** examine the cross-protection among different variant spikes,we initially prepared four chimeric SARS-CoV-2 viruses(Fig.1a),each bearing the spike gene from Alpha,Beta,Gamma,or Epsilon in the backbone of USA-WA1/2020[isolated in January 2020 and defned as wild-type(WT)].

关键词： vaccine cross backbone

中国与北欧国家的视角：全球化与本土化背景下的性别平等促进

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