检索结果-南通市图书馆

In Vitro Reassembly of Tobacco Ribulose-1,5-bisphosphate Carboxylase/Oxygenase from Fully Denatured Subunits

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2006年第10期38卷 737-745页

作者： Zhen-Hua YONG Gen-Yun CHEN* Jiao-Nai SHI Da-Quan XU Institute of Plant Physiology and Ecology,shanghai institutes for biological {3.,chinese academy of {3.,and Graduate School of the chinese academy of sciences,shanghai 20003.,china *** Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences and Graduate School of the Chinese Academy of Sciences Shanghai 200032 China

It has been generally proved impossible to reassemble ribulose-1,5-bisphosphate carboxylase/oxygenase （Rubisco） from fully denatured subunits in vitro in higher plant,because large subunit of fullydenatured Rubisco is liable to precipitate when the denaturant is removed by common methods of directdilution and one-step *** our experiment,the problem of precipitation was resolved by an improvedgradual dialysis method,which gradually decreased the concentration of ***,fully denaturedRubisco subunits still could not be reassembled into holoenzyme using gradual dialysis unless chaperonin 60was *** restored activity of reassembled Rubisco was approximately 8% of natural *** of reassembled Rubisco increased greatly when heat shock protein 70 was present in the *** and Mg2+ were unnecessary for in vitro reassembly of Rubisco,and Mg2+ inhibited the *** reassembly was weakened when ATP,Mg2+ and K+ existed together in the reassembly process.

关键词： Rubisco reassembly gradual dialysis chaperonin 60 Hsp70

Intermittent hypoxia attenuates ischemia/reperfusion induced apoptosis in cardiac myocytes via regulating Bcl-2/Bax expression

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Cell Research 2003年第5期13卷 385-391页

作者： JIAN WEN DONG, HAI FENG ZHU, WEI ZHONG ZHU, HAI LEI DING, TIE MIN MA, ZHAO NIAN ZHOU 1 Laboratory of Hypoxic Cardiovascular Physiology, shanghai institutes for biological {3., chinese academy of {3., shanghai 20003., china.2 Department of Physiology and Pathophysiology, Health Science Center Peking University, Beijing 100083.8} 1Laboratory of Hypoxic Cardiovascular Physiology Shanghai Institutes for Biological SciencesChinese Academy of Sciences Shanghai 200031 China. E-mail: znzhou@*** 1Laboratory of Hypoxic Cardiovascular Physiology Shanghai Institutes for Biological SciencesChinese Academy of Sciences Shanghai 200031 China. E-mail: znzhou@*** 1Laboratory of Hypoxic Cardiovascular Physiology Shanghai Institutes for Biological SciencesChinese Academy of Sciences Shanghai 200031 China. E-mail: znzhou@*** 1Laboratory of Hypoxic Cardiovascular Physiology Shanghai Institutes for Biological SciencesChinese Academy of Sciences Shanghai 200031 China. E-mail: znzhou@*** 1Laboratory of Hypoxic Cardiovascular Physiology Shanghai Institutes for Biological SciencesChinese Academy of Sciences Shanghai 200031 China. E-mail: znzhou@*** 1Laboratory of Hypoxic Cardiovascular Physiology Shanghai Institutes for Biological SciencesChinese Academy of Sciences Shanghai 200031 China. E-mail: znzhou@***

Intermittent hypoxia has been shown to provide myocardial protection against ishemia/reperfusion-induced injury. Cardiac myocyte loss through apoptosis has been reported in ischemia/reperfusion injury. Our aim was to investigate whether intermittent hypoxia could attenuate ischemia/reperfusion-induced apoptosis in cardiac myocytes and its potential mechanisms. Adult male Sprague-Dawley rats were exposed to hypoxia simulated 5000 m in a hypobaric chamber for 6 h/day, lasting 42 days. Normoxia group rats were kept under normoxic conditions. Isolated perfused hearts from both groups were subjected to 3. min of global ischemia followed by 60 min reperfusion. Incidence of apoptosis in cardiac myocytes was determined by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling （TUNEL） and DNA agarose gel electrophoresis. Expressions of apoptosis related proteins, Bax and Bcl-2, in cytosolic and membrane fraction were detected by Western Blotting. After ischemia/reperfusion, enhanced recovery of ca

关键词： intermittent hypoxia apoptosis cardiac myocytes Bax Bcl-2.

Toosendanin interferes with pore formation of botulinum toxin type A in PC12 cell membrane

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2006年第1期27卷 66-70页

作者： Mu-feng LI Yu-liang SHI Institute of Physiology,shanghai institutes for biological {3.,chinese academy of {3.,shanghai 20003.,china InstituteofPhysiology ShanghaiInstitutesforBiologicalSciencesChineseAcademyofSciencesShanghai200031China

Aim:Botulinum neurotoxins (BoNT) abort the process of neurotransmitter re-lease at presynaptic motor nerve terminals,causing muscle *** abilityof botulinum toxin to produce its effect is dependent on the ability of the lightchain to cleave the SNARE proteins associated with transmitter ***-cation of the light chain protease through the heavy chain-formed channel is apivotal step in the intoxication *** (TSN),a triterpenoid de-rivative extracted from a chinese traditional medicine,has been demonstrated tobe an effective cure for experimental *** study was designed to explorethe antibotulismic mechanisms of ***:The inside-out single-channel recording patch-clamp technique was used to record the BoNT/A-in-duced currents in the presence and absence of ***:Channel formationwas delayed and the sizes of the channels were reduced in the TSN-treated PC12cell ***:The antibotulismic effect of TSN might occur viainterference with toxin translocation.

关键词： toosendanin botulinum toxin type A ion channel antibotulismic mechanism single channel recording

Research progress on Drosophila visual cognition in china

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science china(Life sciences) 2010年第3期53卷 374-384页

作者： GUO AiKe1,2,ZHANG Ke1,PENG YueQin1 & XI Wang1 1 Institute of Neuroscience,State Key Laboratory of Neuroscience,shanghai institutes for biological {3.,chinese academy of {3.,shanghai 20003.,china 2 State Key Laboratory of Brain and Cognitive Science,Institute of Biophysics,chinese academy of {3.,Beijing 100101,china 1. Institute of Neuroscience State Key Laboratory of Neuroscience Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China2. State Key Laboratory of Brain and Cognitive Science Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China

Visual cognition,as one of the fundamental aspects of cognitive neuroscience,is generally associated with high-order brain functions in animals and ***,as a model organism,shares certain features of visual cognition in common with mammals at the genetic,molecular,cellular,and even higher behavioral *** learning and memory to decision making,Drosophila covers a broad spectrum of higher cognitive behaviors beyond what we had *** with powerful tools of genetic manipulation in Drosophila,an increasing number of studies have been conducted in order to elucidate the neural circuit mechanisms underlying these cognitive behaviors from a genes-brain-behavior *** goal of this review is to integrate the most important studies on visual cognition in Drosophila carried out in china's Mainland during the last decade into a body of knowledge encompassing both the basic neural operations and circuitry of higher brain function in ***,we consider a series of the higher cognitive behaviors beyond learning and memory,such as visual pattern recognition,feature and context generalization,different feature memory traces,salience-based decision,attention-like behavior,and cross-modal leaning and *** discuss the possible general gain-gating mechanism implementing by dopamine-mushroom body circuit in fly's visual *** hope that our brief review on this aspect will inspire further study on visual cognition in flies,or even beyond.

关键词： Visual invariance recognition visual memory traces feature and context generalization selective attention cross-modality memory integration salience-based decision making

维普期刊数据库

Novel Tumor-associated Antigen of Hepatocellular Carcinoma Defined by Monoclonal Antibody E4-65

在线全文

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2007年第5期39卷 359-365页

作者： Ke ZOU~(1,2), Jihang JU~(1,2), and Hong XIE~~(1*)1 Institute of Biochemist13. and Cell Biology, shanghai institutes for {3. sciences, chinese academy of sciences, shanghai 20003., china. 2 Graduate School of the chinese academy of sciences Beijing 100049, china InstituteofBiochemistryandCellBiology ShanghaiInstitutesforBiologicalSciencesChineseAcademyofSciencesShanghai200031China GraduateSchoolofthechineseacademyofsciences Beijing100049China

A monoclonal antibody, E4-65, produced by immunizing mice with SMMC-7721 cells, a human hepatocellular carcinoma (HCC) cell line, was used to identify and characterize an unreported HCC-associated antigen. Indirect immunofluorescence studies showed that E4-65 antibody reacted with five out of eight HCC cell lines, but not with 10 non-HCC tumor cell lines or a normal liver cell line. Using immunohistochemical examination, E4-65 antigen was detected on the cell membranes and in the cytoplasm of human liver tumor tissues, but was not found in most other tumors, or normal adult or fetal tissues, except for a weakly positive reaction in tissues of the digestive system. Western blot analysis showed that E4-65 antibody bound to a 45 kDa protein in the human HCC cell line and tissue lysates. Enzyme treatment and lectin blotting did not detect the carbohydrate chain in E4-65 antigen. This HCC-associated protein represents a potentially useful target for diagnoses and immunotherapy of human HCC.

关键词： hepatocellular carcinoma immunohistochemistry monoclonal antibody neoplasm antigen

Expression of Hepatitis C Virus E2 Ectodomain in *** and Its Application in the Detection of Anti-E2 Antibodies in Human Sera

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2004年第1期36卷 57-63页

作者： Jing LIU, Xin-Xin ZHANG, Shen-Ying ZHANG, Min LU, Yu-Ying KONG, Yuan WANG, and Guang-Di LI ( State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, shanghai institutes for biological {3., the chinese academy of sciences, shanghai 20003., china 2 Department of Infectious Diseases, Ruijin Hospital, Second Medical University of shanghai, shanghai 200025, china ) State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200031 Chin

The second envelope glycoprotein （E2） of hepatitis C virus has been shown to bind human target cells and has become a major target for the development of anti-HCV vaccines. Anti-E2 antibodies have been suggested to be of clinical significance in diagnosis, treatment and prognosis of hepatitis C. However, large-scale expression and purification of E2 proteins in mammalian cells is difficult. As an alternative, E2 fragment （aa 3.5-73.） with a four-amino-acid mutation （aa 568-571 PCNI to RVTS） was expressed as hexa-histidine-tagged full length protein [E2N73.（m）] in E. coli and purified to over 85% purity. Purified E2N73.（m） was specifically recognized by homologous hepatitis C patient serum in Western blot, suggesting that it displayed E2specific antigenicity. Rabbit antiserum raised against E2N73.（m） recognized E2 glyco-proteins expressed in mammalian cells in Western blot. Purified E2N73.（m） was used to detect anti-E2 antibodies in human sera and showed better specificity and sensitivity than previousl

关键词： hepatitis C virus envelope protein E2 expression and purification Escherichia coll

GABA transporter 1 transcriptional starting site exhibiting tissue specific difference

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Cell Research 2001年第2期11卷 161-163页

作者： JIN XIAO PING, FANG HUANG, NA YANG, BU FENG LU, JIAN FEI, LI HE GUO Institute of Biochemistry and Cell Biology, shanghai institutes for biological Science, {3. academy of sciences, 3.0 Yueyang Road, shanghai 20003., china Institute of Biochemistry and Cell Biology Chinese Academy of Sciences Shanghai.

GABA transporter 1(GAT1) takes important roles in multiple physiological processes through the uptake and release of GABA, but the regulation of GAT1 gene expression in different tissues is rarely known. To address the question, first, 5’ Rapid amplification of cDNA end (RACE) was used to determine GAT1 transcriptional starting sites in neonatal mouse cerebral cortex and intestine, adult mouse brain and adult rat testis. The products of 5’RACE were confirmed by DNA sequencing. We found that the transcript of GAT1 in neonatal mouse cerebral cortex and adult mouse brain starts at the same site (inside of exon 1), while in mouse intestine, GAT1 starts transcription in intron 1, and in rat testis, the transcript of GAT1 has an additional untranslation exon to the 5’ direction.

关键词： GABA transporter 1(GAT1) transcriptional starting site 5’ RACE tissue spedcific difference DNA sequencing.

The involvement of p3. MAPK in transforming growth factor β1-induced apoptosis in murine hepatocytes

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Cell Research 2001年第2期11卷 89-94页

作者： LIAO JIN HUI, JUN SONG CHEN, MIN QIANG CHAI, SHENG ZHAO, JIAN GUO SONG State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, shanghai institutes for biological {3., chinese academy of {3., 3.0 Yue Yang Road, Shan 1State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yue Yang Road Shanghai 200031 China 1State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yue Yang Road Shanghai 200031 China 1State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yue Yang Road Shanghai 200031 China 1State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yue Yang Road Shanghai 200031 China 1State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yue Yang Road Shanghai 200031 China

We reported in this manuscript that TGF-β1 induces apoptosis in AML12 murine hepatocytes, which is associated with the activation of p3. MAPK signaling pathway. SB202190, a specific inhibitor of p3. MAPK, strongly inhibited the TGF-β1-induced apoptosis and PAI-1 promoter activity. Treatment of cells with TGF-β1 activates p3.. Furthermore, over-expression of dominant negative mutant p3. also reduced the TGF-β1-induced apoptosis. The data indicate that the activation of p3. is involved in TGF-β1-mediated gene expression and apoptosis.

关键词： Transforming growth factor β apoptosis p3. hepatocyte signal transduction.

Development of a complex scintillation proximity assay for highthroughput screening of PPAR7 modulators

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2005年第3期26卷 339-344页

作者： Bin WU Jie GAO Ming-wei WANG~2 The National Center for Drug Screening,shanghai Institute of Materia Medica,shanghai institutes for {3. sciences,chinese academy of sciences Graduate School of chinese academy of sciences,shanghai 201203.china The National Center for Drug Screening Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of chinese academy of sciences Shanghai 201203 China

Aim:To develop a complex high-throughput screening （HTS） assay based onscintillation proximity assay （SPA） technology for identification of novel peroxi-some proliferator-activated receptor gamma （PPARγ） ***:Full-length PPARγ and retinoid X receptor alpha （RXRα）,biotinylated PPAR responseelement （PPRE）,[3.]BRL49653.and streptavidin-coated FlashPlate or microbeadwere used to develop an HTS assay based on SPA ***‘ABCDE’method was validated against conventional hydroxyapatite （HA） assay and ap-plied to large-scale screening of 16 000 synthetic compounds and natural ***:（1） IC50values of positive control compounds （BRL49653.andtroglitazone） obtained from the‘ABCDE’method and HA assay were compa-rable and consistent with those reported elsewhere;（2） Approximately 178compounds,showing more than 70% competitive inhibition on BRL49653.bind-ing to PPARγ,were identified initially by the‘ABCDE’method （microbead）;（3.Secondary screening using FlashPlate and cross-reactivity studies with RARα,β,γ and RXRα,β,γ confirmed that 12 compounds possessed specific PPARγ bind-ing properties including 2 with IC50values less than 0.5 μmol/L and novel chemi-cal ***:The‘ABCDE’method using either FlashPlate ormicrobead,is a highly efficient,automatable,and robust tool to screen potentialPPARγ modulators in HTS *** application may be expanded to othernuclear receptors that form heterodimers upon activation.

关键词： peroxisome proliferator-activated receptor gamma retinoid X receptor alpha scintillation proximity assay high-throughput screening