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The bacterial abundance and production in the East China Sea: seasonal variations and relationships with the phytoplankton biomass and production

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Acta Oceanologica Sinica 2014年第9期33卷 166-177页

作者： chen bingzhang HUANG Bangqin XIE Yuyuan GUO Cui SONG Shuqun LI Hongbo LIU Hongbin Fujian Provincial Joint Key Laboratory of Coastal Ecology and Environmental Studies and State Key Laboratory of Marine Environmental Science Xiamen University Division of Life Science Hong Kong University of Science and Technology Key Laboratory of Marine Ecology and Environmental Science Institute of Oceanology Chinese Academy of Sciences National Marine Environmental Monitoring Center State Oceanic Administration

The East China Sea is a productive marginal sea with a wide continental shelf and plays an important role in absorbing atmospheric carbon dioxide and transferring terrigenous organic matter to the open ocean. To investigate the roles of heterotrophic bacteria in the biogeochemical dynamics in the East China Sea, bacterial biomasses （BB） and productions （BP） were measured in four cruises. The spatial distributions of the BB and the BP were highly season-dependent. Affected by the Changjiang River discharge, the BB and the BP were high in shelf waters （bottom depth not deeper than 50 m） and generally decreased offshore in August 2009. In December 2009 to lanuary 2010, and November to December 2010, the BB and the BP were high in waters with medium bottom depth. The onshore-offshore decreasing trends of the BB and the BP also existed in May-June 2011, when the BB was significantly higher than in other cruises in shelf break waters （bottom depth deeper than 50 m but not deeper than 200 m）. The results of generalized additive models （GAM） suggest that the BB increased with the temperature at a range of 8-20~C, increased with the chlorophyll concentration at a range of 0.02-3.00 mg/m3 and then declining, and decreased with the salinity from 28 to 35. The relationship between the temperature and the log-transformed bacterial specific growth rate （SGR） was linear. The estimated temperature coefficient （Q10） of the SGR was similar with that of the phytoplankton growth. The SGR also increased with the chlorophyll concentration. The ratio of the bacterial to phytoplankton production ranged from less than 0.01 to 0.40, being significantly higher in November December 2010 than in May-June 2011. Calculated from the bacterial production and growth efficiency, the bacterial respiration consumed, on average, 59%, 72% and 23% of the primary production in August 2009, November-December 2010, and May-/une 2011, respectively.

关键词： bacterial production generalized additive model chlorophyll temperature East China Sea

Making comparable measurements of bacterial respiration and production in the subtropical coastal waters

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Marine Life Science & Technology 2022年第3期4卷 414-427页

作者： Cui Guo Ying Ke bingzhang chen Shuwen Zhang Hongbin Liu College of Marine Life Sciences Institute of Evolution and Marine BiodiversityFrontiers Science Center for Deep Ocean Multispheres and Earth SystemOcean University of ChinaQingdao 266003China Division of Life Science The Hong Kong University of Science and TechnologyHong Kong SARChina Department of Mathematics and Statistics University of StrathclydeGlasgow G11XHUK Hong Kong Branch of Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou) Hong Kong University of Science and TechnologyHong Kong SARChina College of Life Science South China Normal UniversityGuangzhou 510631China Department of Ocean Science Hong Kong University of Science and TechnologyHong Kong SARChina

Accurate estimates of bacterial carbon metabolic rates are indispensable for understanding the regulation of carbon fluxes in aquatic ***,changes in bacterial growth,production,and cell volume in both pre-filtered and unfiltered seawater during 24 h incubation were *** methodological artifacts during Winkler bacterial respiration(BR)measurements in subtropical Hong Kong coastal waters were *** abundance increased by 3-and 1.8-fold in the pre-filtered and unfiltered seawater after incubation,*** production(BP)and cell volume also showed significant *** with the BR measurements obtained by the Winkler method,the instantaneous free-living BR measurements,after correction,decreased by~70%.The time-integrated free-living BR and BP during 24 h incubation in the pre-filtered sample provided an improved estimate of bacterial growth efficiency,which increased by~52%compared to the common estimations using the noncomparable measurements of integrated free-living BR and instantaneous total *** overestimation of BR also exaggerated the contribution of bacteria to community respiration,affecting the understanding on the metabolic state of the marine ***,the BR estimates by the Winkler method may be more biased in environments with a higher bacterial growth rate and tightly coupled grazing mortality,as well as in those with higher nutrient *** results reveal obvious problems associated with the BR methodology and raise a warning for caution when comparing BP and BR,as well as when making estimations of carbon flow through the complex microbial networks in aquatic ecosystems.

关键词： Bacterial respiration Bacterial growth efficiency Bacterial production Free-living bacteria Winkler method

The importance of picophytoplankton and its top-down control in marine environments

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生态科学 2008年第5期27卷 303-309页

作者： Hongbin Liu bingzhang chen Atmospheric Marine and Coastal Environment (AMCE) Program Hong Kong University of Science and Technology Clear Water Bay Hong Kong Department of Biology Hong Kong University of Science and Technology Clear Water Bay Hong Kong

Phytoplankton are classfied into three groups based on size: microphytoplankton (>20 μm), nanophytoplankton (2-20 μm), and picophytoplankton (<2 μm) (Sieburth et al. 1978) [1]. Picophytoplankton are composed of thr... 详细信息

关键词：海洋环境生态系统物种生态环境

微台阶工法在软弱围岩高风险隧道中的应用研究

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铁道建筑技术 2021年第8期 136-139页

作者：陈兵章中铁二十一局集团第二工程有限公司甘肃兰州730000

软弱围岩条件下隧道施工存在技术难度高、安全风险大、进度缓慢等一系列问题。本文结合微台阶工法在兴隆山隧道施工中的应用,详细介绍微台阶工法施工特点及施工工艺流程,并对应用微台阶工法后兴隆山隧道工程进行效果评价。结果表明,采... 详细信息

软弱围岩条件下隧道施工存在技术难度高、安全风险大、进度缓慢等一系列问题。本文结合微台阶工法在兴隆山隧道施工中的应用,详细介绍微台阶工法施工特点及施工工艺流程,并对应用微台阶工法后兴隆山隧道工程进行效果评价。结果表明,采用微台阶工法可大幅提升隧道施工效率,且可有效控制隧道施工产生的不均匀沉降及收敛变形,减小了塌方概率,为软岩隧道顺利开挖提供保障,并取得了显著的经济效益。

关键词：隧道施工软弱围岩微台阶工法施工工艺效益

大悬挑SRC空间桁架结构施工全过程力学特性分析

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铁道建筑技术 2022年第8期 185-189页

作者：陈兵章中铁二十一局集团第二工程有限公司甘肃兰州730000

为研究大悬挑SRC空间桁架结构在施工全过程中的力学响应,基于广东省某展览馆项目,运用有限元软件对该结构施工吊装全过程进行模拟分析。结果表明:各分析对象内力、应力和竖向位移最值总体上均呈现随施工阶段进行逐渐增大的趋势,仅局部... 详细信息

为研究大悬挑SRC空间桁架结构在施工全过程中的力学响应,基于广东省某展览馆项目,运用有限元软件对该结构施工吊装全过程进行模拟分析。结果表明:各分析对象内力、应力和竖向位移最值总体上均呈现随施工阶段进行逐渐增大的趋势,仅局部存在异常情况;轴力正负最值分别在桁架HJ4、结构柱处产生,弯矩正负最值分别出现在屋面梁、二层梁部位,剪力正负最值均出现在二层梁,应力最值产生于桁架HJ3处;通过对方案A、B进行优选,得出方案A最大优化效果更高的结论,应采取方案A进行结构施工。

关键词：大悬挑SRC结构空间桁架施工全过程临时支撑力学特性方案对比

基于PCI4732的高定位精度光声成像系统设计

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西南交通大学学报 2012年第1期47卷 115-120页

作者：荣健陈炳章钟晓春黄林蒋华北电子科技大学物理电子学院四川成都610031 西南交通大学物理科学与技术学院四川成都610031 弗罗里达大学生物医学工程学院弗罗里达盖恩思维尔33063

为了研究光声成像技术对不同吸收体的成像性能,利用PCI4732和高精度旋转台RT-5-10,在Labview软件设计平台上开发了一套光声成像系统.系统由脉冲激光器、水浸探头和放大器等硬件组成,采集卡通过PCI总线与电脑连接.采用高分辨率滤波反投... 详细信息

为了研究光声成像技术对不同吸收体的成像性能,利用PCI4732和高精度旋转台RT-5-10,在Labview软件设计平台上开发了一套光声成像系统.系统由脉冲激光器、水浸探头和放大器等硬件组成,采集卡通过PCI总线与电脑连接.采用高分辨率滤波反投影重建算法对数据进行处理,得到较高对比度和分辨率的图像.分别采用2.25、5.00和10.00 MHz的超声探头对发丝进行试验,以验证系统的实用性.试验结果显示:采用5.00 MHz的探头比采用2.25和10.00 MHz的探头得到的发丝的图像更清晰,表明对于一定尺寸的吸收体,需要选择一定中心频率的探头,才能得到清晰的图像.

关键词：高定位精度超声探头光声成像技术分辨率 PCI4732

Profile of Dr. Songlin chen

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Science China Life Sciences 2024年第10期67卷 2062-2063页

作者： chen, Songlin Yellow Sea Fisheries Res Inst Qingdao Peoples R China

For decades, Dr. chen has been engaged in fish germplasm preservation, sex control and research focusing on molecular breeding for disease resistance. He discovered the first sex-specific molecular marker of fish(chen et al., 2007),deciphered the first fish genome(Chinese tongue sole) in China, revealed the sex determination mechanism in fish(chen et al., 2014), and created molecular sex control technology. He also discovered the molecular mechanism of metamorphosis in flatfish(Shao et al., 2017) and developed China's first fish disease-resistant breeding gene chip, “YUXIN No.1”.

关键词： TURBOT SCOPHTHALMUS-MAXIMUS GENOMIC SELECTION JAPANESE FLOUNDER SEX-CHROMOSOME CELL-LINE CRYOPRESERVATION EVOLUTION EMBRYOS

用于光声层析成像的虚拟阵列探测及逆拉东变换(英文)

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强激光与粒子束 2013年第3期25卷 783-786页

作者：鲁奎刘立新钟晓春黄林陈炳章荣健电子科技大学物理电子学院成都610054 西南交通大学物理科学与技术学院成都610031

提出用固定孔径的换能器虚拟阵列探测器进行数据采集,该换能器只需对样品进行180°扫描。实验中,使用调QNd:YAG激光器作为光源,激光器输出激光波长为532nm,重复频率为10Hz,脉宽为7ns;使用非聚焦换能器进行光声信号探测,其接收面直径为6... 详细信息

提出用固定孔径的换能器虚拟阵列探测器进行数据采集,该换能器只需对样品进行180°扫描。实验中,使用调QNd:YAG激光器作为光源,激光器输出激光波长为532nm,重复频率为10Hz,脉宽为7ns;使用非聚焦换能器进行光声信号探测,其接收面直径为6mm,中心频率为2.25MHz,接收到光声信号用逆拉东变换进行图像重建。以小白鼠的离体爪子作为成像对象,换能器由旋转电机控制并以2°作为旋转步长进行180°旋转扫描获取实验数据,运用逆拉东变换进行了图像重建。所重建的图像比较清晰地反映了爪子内部的骨骼分布。由此表明,逆拉东变换可以用于光声成像,并且实现了有限角度的光声成像。

关键词：光声成像阵列探测器逆拉东变换图像重建

Effect of Reclamation on the Groundwater-Lake Water Interaction in chen Lake

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Journal of Earth Science 2024年第3期35卷 1083-1086页

作者： Kun Lei Teng Ma Liuzhu chen Zhenxing Li Yu chen School of Environmental Studies State Key Laboratory of Biogeology and Environmental GeologyChina University of GeosciencesWuhan 430078China

0 INTRODUCTION To relieve the short supply of suitable land for residing,cultivating,and commerce,it is accepted to reclaim land from lakes,river estuaries,and coastal regions in many countries(Mancini et al.,2020).The large-scale reclamation activities have an irreversible effect on the mico-geomorphology and hydrodynamic system of seasonally flooded areas(Zhang et al.,2019;Yu et al.,2018).For example,coastal regions reclaimed extended the groundwater flow paths,as well as created groundwater level rise,resulting in water-logging in the reclaimed area(Hu et al.,2008).Similar changes in groundwater flow systems may alter groundwater-lake water interactions,and it can ultimately affect the water cycle in the lake ***,such detailed studies are presently *** addition,natural exchanges between the lake and groundwater systems can be strongly affected by physical hydrological processes(Javadzadeh et al.,2020),chemical properties(Kamtchueng et al.,2015),and bioto behavior(Hancock et al.,2005).

关键词： groundwater coastal logging