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Science China Chemistry 2014年第4期57卷 586-595页

作者： yuAN Wei ZHAO NaNa yu bingran JIANG Hui MA Jie XU FuJian State Key Laboratory of Molecular Oncology Cancer Institute & Hospital Chinese Academy of Medical Sciences State Key Laboratory of Chemical Resource Engineering Key Laboratory of Carbon Fiber and Functional Polymers Ministry of Education College of Materials Science & Engineering Beijing University of Chemical Technology

Efficient local gene transfection on a tissue scaffold is dependent on good cell-adhesion characteristics. In this work, the thermoresponsive gelatin-functionalized polycaprolactone(PCL) films were proposed for improvement of cell adhesion and intelligent recovery of gene-transfected cells. Functional copolymer brushes(PCL-g-P(NIPAAm-co-MAAS)) were first prepared via surface-initiated ATRP of N-isopropylacrylamide(NIPAAm) and methacrylic acid sodium salt(MAAS) from the initiatorfuncationalized PCL surfaces. The pendant carboxyl end-groups of the PCL-g-P(NIPAAm-co-MAAS) surface were subsequently coupled with gelatin via carbodiimide chemistry to produce the thermo-responsive gelatin-functionalized PCL surface. The thermo-responsive gelatin-functionalized PCL film surface can improve cell adhesion and proliferation above the LCST of P(NIPAAm) without destroying cell detachment properties at lower temperatures. The dense transfected cells can be recovered simply by lowering culture temperature. The thermo-responsive gelatin-functionalized PCL films are potentially useful as intelligent adhesion modifiers for directing cellular functions within tissue scaffolds.

关键词： surface functionalization PCL film ATRP gene NIPAAm

Chemiluminescence:From mechanism to applications in biological imaging and therapy

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Aggregate 2021年第6期2卷 43-59页

作者： Zhijia Wang Jiaguo Huang Jingsheng Huang bingran yu Kanyi Pu Fu-Jian Xu Laboratory of Biomedical Materials and Key Lab of Biomedical Materials of Natural Macromolecules(Beijing University of Chemical Technology Ministry of Education)Beijing University of Chemical TechnologyBeijingP.R.China School of Chemical and Biomedical Engineering Nanyang Technological UniversitySingapore Division of Chemistry and Biological Chemistry School of Physical and Mathematical SciencesNanyang Technological UniversitySingapore

Chemiluminescence(CL)is an emission phenomenon induced by chemical *** from the photoluminescence,CL is free from external excitation source,which is expected to show great advantages such as higher signal-to-background ratio(SBR)in bioimaging,and deeper tissue penetration in photodynamic therapy(PDT).This review introduces the theoretical aspects of CL mechanism,such as classification,energy consideration and chemiexcited/photoexcited *** of CL in bioimaging is *** particular,the approaches to modulate the brightness and the wavelength of CL are summarized,which are two fundamental parameters in ***,the application of CL in PDT is *** potential challenges and perspectives of CL in bioimaging and therapy are also discussed.

关键词： chemiluminescence imaging molecular probes photodynamic therapy

Biguanide chitosan microneedles with cell-free DNA scavenging ability for psoriasis therapy

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Bioactive Materials 2024年第3期33卷 497-505页

作者： Zihao Liu yu Wang yuhan Zhang Liufu Hu Bozhi Chen Yang Li Xindong Guo bingran yu Fu-Jian Xu State Key Laboratory of Chemical Resource Engineering Key Lab of Biomedical Materials of Natural Macromolecules(Beijing University of Chemical TechnologyMinistry of Education)and Laboratory of Biomedical MaterialsBeijing University of Chemical TechnologyBeijing 100029China

High levels of cell-free DNA(cfDNA)induce ***,the treatment of psoriasis has the disadvantages of penetration difficulty,suppression of normal immunity,and skin *** this study,biguanide chitosan microneedles(BGC-MNs)were prepared to treat psoriasis by removing cfDNA from the dermis through the skin *** effects of chitosan with different bisguanidine contents on DNA-binding capacity,biocompatibility,and inflammation inhibition were compared,revealing that chitosan containing 20%bisguanidine(BGC2)was found to have the best overall *** vitro,BGC2 effectively cleared cfDNA and inhibited the production of inflammatory ***-MN made from BGC2 had good mechanical and solubility *** vivo,BGC-MNs cleared cfDNA,reduced the level of inflammatory factors in the dermis,and exerted a good therapeutic effect on mice with *** results suggested that BGC-MNs provided a new approach to treating psoriasis in terms of scavenging cfDNA and exerting anti-inflammatory effects.

关键词： Biguanide chitosan cfDNA Microneedles Psoriasis

Reduction-responsive nucleic acid nanocarrier-mediated miR-22 inhibition of PI3K/AKT pathway for the treatment of patient-derived tumor xenograft osteosarcoma

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Bioactive Materials 2023年第10期28卷 376-385页

作者： Dafu Chen Chengyue Lei Weifeng Liu Meiyu Shao Meizhou Sun Jianxun Guo Jingjing Cao Jing-Jun Nie Peng Luo yuwen Luo bingran yu Renxian Wang Shun Duan Fu-Jian Xu Laboratory of Bone Tissue Engineering Beijing Laboratory of Biomedical MaterialsNational Center for OrthopaedicsBeijing Research Institute of Traumatology and OrthopaedicsBeijing Jishuitan HospitalBeijing100035China State Key Laboratory of Chemical Resource Engineering Key Lab of Biomedical Materials of Natural Macromolecules(Beijing University of Chemical Technology)Ministry of EducationBeijing Laboratory of Biomedical MaterialsBeijing University of Chemical TechnologyBeijing100029China Department of Orthopaedic Oncology Surgery Beijing Jishuitan HospitalPeking UniversityBeijing100035China JST Sarcopenia Research Centre Beijing Research Institute of Traumatology and OrthopaedicsBeijing Jishuitan HospitalBeijing100035China

miRNAs are important regulators of gene expression and play key roles in the development of cancer, including osteosarcoma. During the development of osteosarcoma, the expression of miR-22 is significantly downregulated, making miR-22 as a promising therapeutic target against osteosarcoma. To design and fabricate efficient delivery carriers of miR-22 into osteosarcoma cells, a hydroxyl-rich reduction-responsive cationic polymeric nanoparticle, TGIC-CA (TC), was developed in this work, which also enhanced the therapeutic effects of Volasertib on osteosarcoma. TC was prepared by the ring-opening reaction between amino and epoxy groups by one-pot method, which had the good complexing ability with nucleic acids, reduction-responsive degradability and gene transfection performance. TC/miR-22 combined with volasertib could inhibit proliferation, migration and promote apoptosis of osteosarcoma cells in vitro. The anti-tumor mechanisms were revealed as TC/ miR-22 and volasertib could inhibit the PI3K/Akt signaling pathway synergistically. Furthermore, this strategy showed outstanding tumor suppression performance in animal models of orthotopic osteosarcoma, especially in patient-derived chemo-resistant and chemo-intolerant patient-derived xenograft (PDX) models, which reduced the risk of tumor lung metastasis and overcame drug resistance. Therefore, it has great potential for efficient treatment of metastasis and drug resistance of osteosarcoma by the strategy of localized, sustained delivery of miR-22 using the cationic nanocarriers combined with non-traditional chemotherapy drugs.

关键词： Nucleic acid delivery Nanoparticle Gene therapy Osteosarcoma Responsive Patient-Derived Xenograft(PDX)

Flexible electrostatic hydrogels from marine organism for nitric oxide-enhanced photodynamic therapy against multidrug-resistant bacterial infection

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Science China Materials 2022年第10期65卷 2850-2860页

作者： yujie Sun Rong-Lai Wen Dan yu Yiwen Zhu Liang Zheng Xiangdong Liu Haoran Wang bingran yu Fu-Jian Xu State Key Laboratory of Chemical Resource Engineering Key Lab of Biomedical Materials of Natural Macromolecules(Beijing University of Chemical TechnologyMinistry of Education)and Laboratory of Biomedical MaterialsCollege of Materials Science and EngineeringBeijing University of Chemical TechnologyBeijing 100029China School of Materials Science and Engineering Zhejiang Sci-Tech UniversityXiasha Higher Education ZoneHangzhou 310018China

The abuse of antibiotics in treating microbial infections has led to the emergence and prevalence of drugresistant ***,the development of novel antibacterial materials is attracting increasing ***,a series of flexible electrostatic hydrogels with excellent antibacterial ability were constructed using a mixture of nitric oxide(NO)-releasing nitrated chitosan(CSNO)and mesotetra(4-carboxyphenyl)porphine(TCPP)with salmon sperm DNA(ssDNA)*** cultured with gram-negative bacteria under solar simulator irradiation,TCPP-CSNO_(m)ssDNA_(n) hydrogels released reactive oxygen species(ROS)and NO to produce peroxynitrite ions(ONOO^(−)).ONOO−is efficient at killing bacteria,thereby improving the antimicrobial ability of photodynamic therapy against gram-negative *** hydrogels exhibited powerful antibacterial activity in vivo when used to treat skin infections caused by drugresistant bacteria,making them a promising candidate for clinical applications.A string of antibacterial hydrogels that release ROS and NO synergistically can bring new possibilities for effectively killing drug-resistant bacteria and be of great value in anti-infection wound dressings and other applications.

关键词： electrostatic hydrogel drug-resistant bacteria peroxynitrite photodynamic therapy

Infection-responsive polysaccharide-based drug-loaded nano-assembly for dual-modal treatment against drug-resistant bacterial lung infection

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BMEMat(BioMedical Engineering Materials) 2024年第3期2卷 123-136页

作者： Lin Han Zhonghua yuan Hui-Min Ren Weizhuo Song Ruonan Wu Jie Li Zhaoyan Guo bingran yu Shun Duan Fu-Jian Xu State Key Laboratory of Chemical Resource Engineering Key Lab of Biomedical Materials of Natural Macromolecules(Beijing University of Chemical TechnologyMinistry of Education)Beijing Laboratory of Biomedical MaterialsBeijing University of Chemical TechnologyBeijingChina Beijing Research Institute of Chemical Industry SinopecBeijingChina

The escalating issue of lung infections induced by multi-drug resistant(MDR)bacteria is threatening human ***,the development of efficient drug delivery systems is essential to eliminate MDR bacterial lung infections ***,we designed inhalable drug-loaded nano-assemblies by the electrostatic interaction between negatively charged sodium alginate and a positively charged antibacterial polymer,quaternized polyethyleneimine(QPEI-C_(6)),as well as a kind of typical antibiotic for therapy of lung infection,azithromycin(AZT).By adjusting the feed ratios,we optimized the size of the nano-assembly to approximately 200 nm(STQ_(12)),which was beneficial for penetration through the mucus layer and *** the slightly acidic environment of the infected site,the nano-assembly could dissemble responsively and release AZT and QPEI-C_(6).Because of the combined bactericidal effect,STQ_(12)exhibited high bactericidal efficiency against MDR *** animal experiments,STQ_(12)showed notable efficacy against MDR bacterial lung *** transcriptomic results showed that the main effects of STQ_(12)against bacteria were through influencing the bacterial cell components and metabolic processes,and affecting their growth and *** work provides a promising strategy to treat MDR bacterium-induced lower respiratory tract infections.

关键词： antibacterial infection responsiveness multi-drug resistant bacteria nano-assembly polysaccharide

光动力抗菌聚合物功能化钛片用于骨科植入体抗感染

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中华骨与关节外科杂志 2024年第7期17卷 641-647页

作者：聂井君高敏政朱艺文俞丙然陈大福首都医科大学附属北京积水潭医院北京市创伤骨科研究所国家骨科医学中心北京100035 北京化工大学材料科学与工程学院生物医用材料北京实验室北京100029

目的:评估表面修饰光动力抗菌聚合物钛片(Ti-PGMB)植入体的抗感染性能。方法:用活性氧(ROS)荧光探针验证在光照条件下Ti-PGMB植入体产生ROS,分别建立耐甲氧西林金黄色葡萄球菌(MRSA)生物膜模型及植入体部位感染动物模型评估Ti-PGMB抗细... 详细信息

目的:评估表面修饰光动力抗菌聚合物钛片(Ti-PGMB)植入体的抗感染性能。方法:用活性氧(ROS)荧光探针验证在光照条件下Ti-PGMB植入体产生ROS,分别建立耐甲氧西林金黄色葡萄球菌(MRSA)生物膜模型及植入体部位感染动物模型评估Ti-PGMB抗细菌感染的效果。将小鼠随机分为钛片对照(Ti)组、Ti-PGMB无光照(Ti-PGMB-L)组和Ti-PGMB光照(Ti-PGMB+L)组,每组各10只。Ti组植入钛片,Ti-PGMB+L组和Ti-PGMB-L组植入Ti-PGMB。在植入位置接种等量MRSA细菌悬液(5×10^(7)CFU/mL),24 h后对Ti-PGMB+L组连续光照干预7 d,Ti组和Ti-PGMB-L组不进行其他干预措施。观察3组小鼠植入体部位缝合处切口恢复情况,统计干预3 d、7 d后3组小鼠植入体附近组织匀浆中MRSA数量,并通过酶联免疫吸附实验(ELISA)测定组织匀浆中炎症因子的水平,通过苏木精-伊红染色评估组织炎症感染的程度。结果:在光照条件下,Ti-PGMB植入体能够产生ROS并可消散成熟生物膜。在体内抗感染实验中,干预3d、7d后,Ti-PGMB-L组细菌存活率与Ti组(100%)比较,差异均无统计学意义(P均>0.05);而Ti-PGMB+L组干预3 d、7 d后细菌存活率与Ti组(100%)比较,差异均有统计学意义(P均<0.05)。干预7 d后,Ti-PGMB+L组组织匀浆中的白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)炎症因子水平低于Ti-PGMB-L组和Ti组,差异均有统计学意义(P均<0.05)。结论:Ti-PGMB利用其可调控的光动力效应生成ROS,从而消杀感染性细菌及其形成的生物膜。Ti-PGMB能够抑制以MRSA为代表的植入式医疗器械感染、减少炎症反应,具有重要的临床价值。

关键词：光动力抗菌表面功能化骨科植入体

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光动力抗菌高分子材料研究进展

中国材料进展 2022年第7期41卷 508-519页

作者：孙玉洁高敏政朱艺文郑良俞丙然徐福建北京化工大学材料科学与工程学院北京100029

细菌感染是目前人类面临的最为严重的健康问题之一,常规抗生素治疗面临着细菌耐药性等问题,光动力抗菌疗法因具有抗菌谱广、非侵入性以及不易引发耐药性的特点而广受关注,但传统小分子光敏剂由于水溶性较差、缺乏组织靶向性以及对正常... 详细信息

细菌感染是目前人类面临的最为严重的健康问题之一,常规抗生素治疗面临着细菌耐药性等问题,光动力抗菌疗法因具有抗菌谱广、非侵入性以及不易引发耐药性的特点而广受关注,但传统小分子光敏剂由于水溶性较差、缺乏组织靶向性以及对正常组织细胞存在光照毒性等缺点而限制了后续的生物应用。首先介绍了光动力抗菌治疗的基本原理,然后概述了广谱性光动力抗菌高分子材料、细菌靶向性光动力高分子材料、微环境响应性光动力高分子材料这几种光动力材料的研究进展,并对每种光动力治疗材料的构建进行了简要分析,最后总结了目前构建新型聚合物基光动力抗菌材料所面临的主要问题,提出了未来构建新型光动力抗菌材料的发展方向,希望为我国光动力抗菌治疗的发展提供参考。

关键词：聚合物材料光动力抗菌治疗广谱性细菌靶向微环境响应

维普期刊数据库博看期刊

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钛镍形状记忆合金冷拉拔的加工硬化及再结晶

稀有金属 2015年第8期39卷 680-685页

作者：于孟牛中杰毛江虹薛飒贾兵然西安思维金属材料有限公司陕西西安710021

通过真空感应熔炼制备了Ti-50.9Ni（%,原子分数,下同）形状记忆合金铸锭,铸锭经锻造、轧制及热拉拔至Φ2.0 mm线材,对其退火后进行不同拉拔变形量的冷拉拔,并对不同冷拉拔变形量的丝材进行500-800℃退火。借助拉伸实验、X射线衍射（XRD... 详细信息

通过真空感应熔炼制备了Ti-50.9Ni（%,原子分数,下同）形状记忆合金铸锭,铸锭经锻造、轧制及热拉拔至Φ2.0 mm线材,对其退火后进行不同拉拔变形量的冷拉拔,并对不同冷拉拔变形量的丝材进行500-800℃退火。借助拉伸实验、X射线衍射（XRD）和金相显微镜（OM）等手段研究了Ti-50.9Ni形状记忆合金冷拉拔的加工硬化速率和再结晶,得到了Ti-50.9Ni形状记忆合金冷拉拔加工硬化规律及退火温度对冷拉拔Ti-50.9Ni合金显微组织的影响。研究结果表明,Ti-50.9Ni合金的加工硬化速率较高,平均加工硬化速率可达20 MPa·%-1以上,其加工硬化速率dσb/dε曲线分为3个区域。随着冷拉拔变形量的增加,加工硬化速率dσb/dε先降低,然后升高,再降低,在冷拉拔真应变ε分别为0和0.27时出现了极大值,其原因是Ti-50.9Ni合金冷加工时马氏体的产生、生长及相互交织,以及马氏体量随加工量的变化。进行充分冷拉拔变形后的Ti-50.9Ni形状记忆合金的纤维组织在600℃（约为0.55Tm）退火时发生再结晶现象,在700℃时晶粒将发生长大现象,且Ti-50.9Ni合金发生再结晶的临界变形量在10%-20%之间。因此,冷拉拔加工的Ti-50.9Ni形状记忆合金的退火温度宜在600-700℃之间选择。

关键词：钛镍形状记忆合金冷拉拔加工硬化再结晶

Ni含量和第三元素对退火态钛镍合金相变的影响

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稀有金属 2016年第9期40卷 877-881页

作者：于孟薛飒贾兵然毛江虹牛中杰西安思维金属材料有限公司陕西西安710021

采用差示扫描量热仪（DSC）分析了不同镍含量（49.7%,50.0%,50.5%,50.8%,50.9%;原子分数）及加入第三元素（Fe,Cu）的退火态钛镍合金的相变特征。结果表明：当Ni含量在49.7%~50.9%范围内变化时,退火态二元钛镍合金相变只存在B2B19＇... 详细信息

采用差示扫描量热仪（DSC）分析了不同镍含量（49.7%,50.0%,50.5%,50.8%,50.9%;原子分数）及加入第三元素（Fe,Cu）的退火态钛镍合金的相变特征。结果表明：当Ni含量在49.7%~50.9%范围内变化时,退火态二元钛镍合金相变只存在B2B19＇一阶段相变。加入3%Fe元素后,其退火态相变类型为B19＇RB2二阶段相变。加入5%Cu元素后,其退火态相变类型与二元钛镍合金相似,只出现B2B19＇一阶段相变。对于二元钛镍合金,其各相变温度随着镍含量的增加近似呈线性降低,相变滞后ΔTp随着镍含量的增加而减小,相变滞后ΔTs随着镍含量的增加先增大再减小。马氏体相变峰宽ΔTM和逆马氏体相变峰宽ΔTA在Ni含量为50.2%~50.4%时取得最小值。退火态二元钛镍合金的相变潜热ΔH随着镍含量的增加而下降。加入3%Fe元素的钛镍合金,其相变潜热ΔH为3~5 J·g-1,相转变更易进行。加入5%Cu元素的钛镍合金的相变潜热ΔH与等原子比二元钛镍合金相当,其相变峰宽ΔTM和ΔTA皆低于二元钛镍合金,相变滞后ΔTs和ΔTp更小,分别可达到8.5和24.3℃,更适合制作高精度、快响应热驱动系统。

关键词：成分钛镍形状记忆合金相变