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MicroRNA-7 regulates glioblastoma cell invasion via targeting focal adhesion kinase expression

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Chinese Medical Journal 2011年第17期124卷 2616-2621页

作者： WU De-gang WANG Ying-yi FAN Li-gang LUO Hui han bin sun Li-hua WANG Xie-feng ZhanG Jun-xia CAO Lei WANG Xi-rui YOU Yong-ping LIU Ning Department of Neurosurgery First Affiliated Hospital of Nanjing Medical University Nanjing Jiangsu 210029 China

Background Invasion growth is the most characteristic biological phenotype of glioblastoma, but the molecular mechanism in glioma cell invasion is poorly understood. Recent data have showed that microRNA plays an essential role in tumor invasion. Our study aimed to explore the mechanism of miR-7 involved in the control of glioblastoma cell invasion. Methods Glioma cell invasion was evaluated by transwell and scratch assays after up-regulation of miR-7 using miR-7 mimics in U87 and U251 cells. Luciferase reporter assay was used to determine focal adhesion kinase （FAK） as a target of miR-7. The levels of miR-7, matrix metalloproteinases （MMP）-2 and MMP-9 mRNA were detected by PCR assay, and the levels of FAK, MMP-2, MMP-9, total and phosphorylation serine/threonine kinase （AKT）, and extracellular signal-regulated kinase （ERK） 1/2 were measured by Western blotting analysis. Results Over-expression of miR-7 inhibited the invasion and migration activity of U87 and U251 cells. And up-regulation of miR-7 reduced FAK protein expression, Further, luciferase reporter assay showed that miR-7 modulated FAK expression directly by binding 3＇UTR of FAK mRNA. In addition, miR-7 repressed p-ERK1/2 and p-AKT level, MMP-2 and MMP-9 expression. Finally, the inverse relationship between FAK and miR-7 expression was certificated in human glioma tissues. Conclusion To our knowledge, these data indicate for the first time that miR-7 directly regulates cell invasion by targeting FAK in glioblastoma and that miR-7 could be a potential therapeutic target for glioblastoma intervention.

关键词： microRNA glioblastoma cell invasion focal adhesion kinase

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从燕尾海角的小木屋看设计中的理性与感性

家具与室内装饰 2018年第2期25卷 110-111页

作者：孙迟韩斌沈阳建筑大学设计艺术学院辽宁沈阳110168

社会的持续发展,让人们在室内设计中越来越推崇"功能主义",为实现设计的"功能主义"并使设计达到最优化这一目的,注重设计的过程与方法是十分必要的,文章从感性与理性的角度出发结合设计过程的特点指出设计的过程是一个理性与感性交替相... 详细信息

社会的持续发展,让人们在室内设计中越来越推崇"功能主义",为实现设计的"功能主义"并使设计达到最优化这一目的,注重设计的过程与方法是十分必要的,文章从感性与理性的角度出发结合设计过程的特点指出设计的过程是一个理性与感性交替相互融合的不断循环上升的过程,通过分析勒·柯布西耶的设计理念与其晚期作品燕尾海角的小木屋的设计过程,进一步对理性思维与感性思维在设计中的运用进行了归纳总结,旨在为了更好地提升设计的质量献力。

关键词：感性理性设计过程柯布西耶燕尾海角的小木屋

Deep eutectic solvents for separation and purification applications in critical metal metallurgy:Recent advances and perspectives

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 1-19页

作者： Shuo Chen Shengpeng Su Yanfang Huang bingbing Liu Hu sun Shuzhen Yang Guihong han School of Chemical Engineering Zhengzhou UniversityZhengzhou 450001China Zhongyuan Critical Metals Laboratory Henan ProvinceZhengzhou 450001China

Solvent extraction,a separation and purification technology,is crucial in critical metal *** solvents commonly used in solvent extraction exhibit disadvantages,such as high volatility,high toxicity,and flammability,causing a spectrum of hazards to human health and environmental *** solvents have been recognized as potential alternatives to these harmful organic *** the past two decades,several neoteric solvents have been proposed,including ionic liquids(ILs)and deep eutectic solvents(DESs).DESs have gradually become the focus of green solvents owing to several advantages,namely,low toxicity,degradability,and low *** this critical review,their classification,formation mechanisms,preparation methods,characterization technologies,and special physicochemical properties based on the most recent advancements in research have been systematically ***,the major separation and purification applications of DESs in critical metal metallurgy were comprehensively ***,future opportunities and challenges of DESs were explored in the current research *** conclusion,this review provides valuable insights for improving our overall understanding of DESs,and it holds important potential for expanding separation and purification applications in critical metal metallurgy.

关键词： deep eutectic solvents preparations properties separation and purification critical metal metallurgy

Flexible Graphene Field‑Effect Transistors and Their Application in Flexible Biomedical Sensing

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Nano-Micro Letters 2025年第2期17卷 252-313页

作者： Mingyuan sun Shuai Wang Yanbo Liang Chao Wang Yunhong Zhang Hong Liu Yu Zhang Lin han Institute of Marine Science and Technology Shandong UniversityQingdao 266237ShandongPeople’s Republic of China State Key Laboratory of Crystal Materials Shandong UniversityJinan 250100ShandongPeople’s Republic of China School of Integrated Circuits Shandong UniversityJinan 250100ShandongPeople’s Republic of China Shandong Engineering Research Center of Biomarker and Artificial Intelligence Application Jinan250100ShandongPeople’s Republic of China

Flexible electronics are transforming our lives by making daily activities more *** to this innovation are field-effect transistors(FETs),valued for their efficient signal processing,nanoscale fabrication,low-power consumption,fast response times,and ***,known for its exceptional mechanical properties,high electron mobility,and biocompatibility,is an ideal material for FET channels and *** combination of graphene and FETs has given rise to flexible graphene field-effect transistors(FGFETs),driving significant advances in flexible electronics and sparked a strong interest in flexible biomedical ***,we first provide a brief overview of the basic structure,operating mechanism,and evaluation parameters of FGFETs,and delve into their material selection and patterning *** ability of FGFETs to sense strains and biomolecular charges opens up diverse application *** specifically analyze the latest strategies for integrating FGFETs into wearable and implantable flexible biomedical sensors,focusing on the key aspects of constructing high-quality flexible biomedical ***,we discuss the current challenges and prospects of FGFETs and their applications in biomedical *** review will provide valuable insights and inspiration for ongoing research to improve the quality of FGFETs and broaden their application prospects in flexible biomedical sensing.

关键词： Flexible Graphene Field-effect transistor Wearable Implantable Biosensor

Peripheral mitochondrial DNA as a neuroinflammatory biomarker for major depressive disorder

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Neural Regeneration Research 2025年第6期20卷 1541-1554页

作者： Jinmei Ye Cong Duan Jiaxin han Jinrong Chen Ning sun Yuan Li Tifei Yuan Daihui Peng Division of Mood Disorder Shanghai Mental Health CenterShanghai Jiao Tong University School of MedicineShanghaiChina Department of Psychiatry First Hospital of Shanxi Medical UniversityTaiyuanShanxi ProvinceChina Shanghai Key Laboratory of Psychotic Disorders Brain Health InstituteNational Center for Mental DisordersShanghai Mental Health CenterShanghai Jiao Tong University School of MedicineShanghaiChina Co-Innovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina

In the pathogenesis of major depressive disorder, chronic stress-related neuroinflammation hinders favorable prognosis and antidepressant response. Mitochondrial DNA may be an inflammatory trigger, after its release from stress-induced dysfunctional central nervous system mitochondria into peripheral circulation. This evidence supports the potential use of peripheral mitochondrial DNA as a neuroinflammatory biomarker for the diagnosis and treatment of major depressive disorder. Herein, we critically review the neuroinflammation theory in major depressive disorder, providing compelling evidence that mitochondrial DNA release acts as a critical biological substrate, and that it constitutes the neuroinflammatory disease pathway. After its release, mitochondrial DNA can be carried in the exosomes and transported to extracellular spaces in the central nervous system and peripheral circulation. Detectable exosomes render encaged mitochondrial DNA relatively stable. This mitochondrial DNA in peripheral circulation can thus be directly detected in clinical practice. These characteristics illustrate the potential for mitochondrial DNA to serve as an innovative clinical biomarker and molecular treatment target for major depressive disorder. This review also highlights the future potential value of clinical applications combining mitochondrial DNA with a panel of other biomarkers, to improve diagnostic precision in major depressive disorder.

关键词： biomarker cytokine exosomes inflammasome major depressive disorder microglia mitochondrial DNA mitochondrial dysfunction neuroinflammation Toll-like receptor

经皮椎间孔镜下与椎板间开窗椎间盘切除术治疗腰椎间盘突出症的Meta分析

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中国矫形外科杂志 2017年第3期25卷 236-242页

作者：任佳彬韩雪馨孙兆忠任强李瑞滨州医学院附属医院山东省滨州市256603

[目的]对经皮椎间孔镜下椎间盘切除术(percutaneous transforaminal endoscopic discectomy,PTED)与椎板间开窗椎间盘切除术治疗腰椎间盘突出症(LDH)的疗效进行系统评价。[方法]计算机检索建库至2016年4月Cochrane Library、Pub Med、Medline、Academic Search Premier、中国生物医学文献数据库、中国知网、维普和万方数据库文献,同时手工检索辅助,收集关于PTED和椎板间开窗椎间盘切除术治疗LDH的随机对照试验。根据纳入与排除标准进行文献筛选、质量评价和数据提取后,应用Revman 5.3软件对各项指标进行Meta分析。[结果]最终纳入10篇文献708例观察对象,其中PTED组349例,椎板间开窗组359例。分析结果显示,PTED组与椎板间开窗组在手术时间、术后疗效评分及优良率方面差异无统计学意义,而在切口长度、出血量、术后卧床时间及住院时间方面PTED组结果更优,差异具有统计学意义。[结论]PTED治疗腰椎间盘突出症与椎板间开窗术效果相当,但PTED具有出血少、创伤小及恢复快等优势。不过因受限于纳入研究的数量和质量,上述结论仍需更多高质量长期随访的随机对照试验进一步验证。

关键词：腰椎间盘突出症椎间孔镜椎板间开窗 Meta分析

Trends of alkaline phosphatase to prealbumin ratio in patients with hepatitis B linked to hepatocellular carcinoma development

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World Journal of Gastroenterology 2025年第2期31卷 42-51页

作者： Wen-Chong Zhen Jing sun Xue-Ting Bai Qian Zhang Zi-han Li Yi-Xin Zhang Rong-Xuan Xu Wei Wu Zhi-han Yao Chun-Wen Pu Xiao-Feng Li School of Public Health Dalian Medical UniversityDalian 116044Liaoning ProvinceChina Graduate School Dalian Medical UniversityDalian 116044Liaoning ProvinceChina Dalian Public Health Clinical Center Dalian Municipal Research Institute for Public HealthDalian 116001Liaoning ProvinceChina Department of Epidemiology and Health Statistics Dalian Medical UniversityDalian 116044Liaoning ProvinceChina

BACKGROUND Chronic hepatitis B often progresses silently toward hepatocellular carcinoma(HCC),a leading cause of mortality *** detection of HCC is crucial,yet *** To investigate the role of dynamic changes in alkaline phosphatase to prealbumin ratio(APR)in hepatitis B progression to *** Data from 4843 patients with hepatitis B(January 2015 to January 2024)were *** incidence rates in males and females were compared using the log-rank *** were evaluated using Kaplan–Meier *** Linear Mixed-Effects Model was applied to track the fluctuation of APR levels over ***,Joint Modeling of Longitudinal and Survival data was employed to investigate the temporal relationship between APR and HCC *** The incidence of HCC was higher in *** ensure the model’s normality assumption,this study applied a logarithmic transformation to APR,yielding *** levels were higher in females(t=5.26,P<0.01).A 1-unit increase in ratio correlated with a 2.005-fold higher risk of HCC in males(95%CI:1.653-2.431)and a 2.273-fold higher risk in females(95%CI:1.620-3.190).CONCLUSION Males are more prone to HCC,while females have higher APR *** no baseline APR link,rising APR indicates a higher HCC risk.

关键词： Alkaline phosphatase to prealbumin ratio Chronic hepatitis B patients Hepatocellular carcinoma Retrospective cohort study Linear Mixed-Effect Mode Joint Modelling of Longitudinal and Survival data

Confinement characteristic of primary electrons with the variation of channel width in the discharge chamber of annular ion thruster

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Chinese Journal of Aeronautics 2021年第5期34卷 79-92页

作者： Chang LU Guangqing XIA bin sun Yajie han State Key Laboratory of Structural Analysis for Industrial Equipment Dalian University of TechnologyDalian 116024China Key Laboratory of Advanced Technology for Aerospace Vehicles of Liaoning Province Dalian University of TechnologyDalian 116024China

In this paper,the confinement characteristic of primary electrons in the non-axisymmetric discharge chamber of annular ion thruster is investigated by a three-dimensional(3 D)non-self-consistent particle tracking model with Monte Carlo Collision(MCC)*** results show that:The density of primary electrons upstream of ion optics on cathode axis is about 5–50 times higher than that of the other side,which means that the density of primary electrons is obviously *** channel width has a significant effect on the average density and uniformity of primary *** average density can be increased by nearly 1.5 times under the appropriate channel width,meanwhile,the variance of density distribution can be reduced by more than 2 *** is because that increasing the channel width can improve the average confinement length of primary electrons ***,there is an infection point in the increase of primary electron average confinement length with the channel width,which caused by the significant change of magnetic field *** the case after the inflection point,primary electron mainly(more than 50%)moves to the channel center,which makes the average confinement length of primary electrons and their number in the discharge chamber increase largely.

关键词： Annular ion thruster Average confinement length Electric propulsion Ion thruster Primary electrons

Impacts of Nutlin-3a and exercise on murine double minute 2-enriched glioma treatment

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Neural Regeneration Research 2025年第4期20卷 1135-1152页

作者： Yisheng Chen Zhongcheng Fan Zhiwen Luo Xueran Kang Renwen Wan Fangqi Li Weiwei Lin Zhihua han Beijie Qi Jinrong Lin Yaying sun Jiebin Huang Yuzhen Xu Shiyi Chen Department of Sport Medicine Huashan HospitalFudan UniversityShanghaiChina Department of Orthopedic Surgery Hainan Province Clinical Medical CenterHaikou Affiliated Hospital of Central South University Xiangya School of MedicineHaikouHainan ProvinceChina Department of Basic Medicine Shanghai Jiao Tong University School of MedicineShanghai Jiao Tong UniversityShanghaiChina Department of Neurosurgery Second Affiliated Hospital of Zhejiang University School of MedicineZhejiang UniversityHangzhouZhejiang ProvinceChina Department of Orthopedics Shanghai General HospitalSchool of Medicine Shanghai Jiao Tong UniversityShanghaiChina Department of Infectious Diseases Children’s Hospital of Fudan UniversityNational Children’s Medical CenterShanghaiChina Department of Rehabilitation The Second Affiliated Hospital of Shandong First Medical UniversityTaianShandong ProvinceChina

Recent research has demonstrated the impact of physical activity on the prognosis of glioma patients,with evidence suggesting exercise may reduce mortality risks and aid neural *** role of the small ubiquitin-like modifier(SUMO)protein,especially post-exercise,in cancer progression,is gaining attention,as are the potential anti-cancer effects of *** used machine learning to create the exercise and SUMO-related gene signature(ESLRS).This signature shows how physical activity might help improve the outlook for low-grade glioma and other *** demonstrated the prognostic and immunotherapeutic significance of ESLRS markers,specifically highlighting how murine double minute 2(MDM2),a component of the ESLRS,can be targeted by *** underscores the intricate relationship between natural compounds such as nutlin-3 and immune *** comprehensive CRISPR screening,we validated the effects of specific ESLRS genes on low-grade glioma *** also revealed insights into the effectiveness of Nutlin-3a as a potent MDM2 inhibitor through molecular docking and dynamic ***-3a inhibited glioma cell proliferation and activated the p53 *** efficacy decreased with MDM2 overexpression,and this was reversed by Nutlin-3a or *** using a low-grade glioma mouse model highlighted the effect of physical activity on oxidative stress and molecular pathway ***,both physical exercise and Nutlin-3a administration improved physical function in mice bearing tumors derived from MDM2-overexpressing *** results suggest the potential for Nutlin-3a,an MDM2 inhibitor,with physical exercise as a therapeutic approach for glioma *** research also supports the use of natural products for therapy and sheds light on the interaction of exercise,natural products,and immune regulation in cancer treatment.

关键词： exercise and SUMO-related gene signatures(ESLRS) glioblastoma management low-grade glioma natural bioactives neural regeneration physical exercise