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17β-estradiol inhibits TGF-β-induced collagen gel contraction mediated by human Tenon fibroblasts via Smads and MAPK signaling pathways

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International Journal of Ophthalmology(English edition) 2023年第9期16卷 1441-1449页

作者： Cheng-Cheng Yang Meng-Jie liu Yun-Ze-Peng li Zheng-Hua Xu Yang liu Zi-Han Guo bin-hui li Xiu-Xia Yang Department of Ophthalmology the Fifth Affiliated HospitalSun Yat-sen UniversityZhuhai 519000Guangdong ProvinceChina Eye Institute of Xiamen University Fujian Provincial Key Laboratory of Ophthalmology and Visual ScienceSchool of MedicineXiamen UniversityXiamen 361000Fujian ProvinceChina

AIM:To investigate the impact of 17β-estradiol on the collagen gels contraction(CGC)and inflammation induced by transforming growth factor(TGF)-βin human Tenon fibroblasts(HTFs).METHODS:HTFs were three-dimensionally cultivated in type I collagen-generated gels with or without TGF-β(5 ng/mL),17β-estradiol(12.5 to 100μmol/L),or progesterone(12.5 to 100μmol/L).Then,the collagen gel diameter was determined to assess the contraction,and the development of stress fibers was analyzed using immunofluorescence *** and gelatin zymography assays were used to analyze matrix metalloproteinases(MMPs)and tissue inhibitors of metalloproteinases(TIMPs)being released into culture ***-linked immunosorbent assay(EliSA)and reverse transcription-quantitative polymerase chain reaction(RT-PCR)were used to detect interleukin(IL)-6,monocyte chemoattractant proteins(MCP)-1,and vascular endothelial growth factor(VEGF)in HTFs at the translational and transcriptional *** phosphorylation levels of Sma-and Mad-related proteins(Smads),mitogen-activated protein kinases(MAPKs),and protein kinase B(AKT)were measured by *** analysis was performed using either the Tukey-Kramer test or Student’s unpaired t-test to compare the various ***:The CGC caused by TGF-βin HTFs was significantly inhibited by 17β-estradiol(25 to 100μmol/L),and a statistically significant difference was observed when comparing the normal control group with 17β-estradiol concentrations exceeding 25μmol/L(P<0.05).The suppressive impact of 17β-estradiol became evident 24h after administration and peaked at 72h(P<0.05),whereas progesterone had no ***,17β-estradiol attenuated the formation of stress fibers,and the production of MMP-3 and MMP-1 in HTFs stimulated by TGF-β.The expression of MCP-1,IL-6,and VEGF mRNA and protein in HTFs were suppressed by 100μmol/L 17β-estradiol(P<0.01).Additionally,the phosphorylation of Smad2 Smad3,p38,and extracellula

关键词： Tenon fibroblasts transforming growth factor-β 17β-estradiol fibrosis wound healing

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Semaphorin 7A impairs barrier function in cultured human corneal epithelial cells in a manner dependent on nuclear factor-kappa B

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International Journal of Ophthalmology(English edition) 2024年第3期17卷 444-453页

作者： Cheng-Cheng Yang Xiu-Xia Yang Xiao-Jing Zhao Heng Wang Zi-Han Guo Kai Jin Yang liu bin-hui li Department of Ophthalmology the Fifth Affiliated HospitalSun Yat-sen UniversityZhuhai 519000Guangdong ProvinceChina Department of Ophthalmology Hangzhou TCM Hospital Affiliated to Zhejiang Chinese Medical UniversityHangzhou 310006Zhejiang ProvinceChina Eye Institute of Xiamen University Fujian Provincial Key Laboratory of Ophthalmology and Visual ScienceSchool of MedicineXiamen UniversityXiamen 361005Fujian ProvinceChina

●AIM:To evaluate the role of semaphorin 7A(Sema7A)and its associated regulatory mechanisms in modulating the barrier function of cultured human corneal epithelial cells(HCEs).●METHODS:Barrier models of HCEs were treated with recombinant human Sema7A at concentrations of 0,125,250,or 500 ng/mL for 24,48,or 72h in *** electrical resistance(TEER)as well as Dextran-fluorescein isothiocyanate(FITC)permeability assays were conducted to assess barrier *** quantify tight junctions(TJs)such as occludin and zonula occludens-1(ZO-1)at the mRNA level,reverse transcriptionpolymerase chain reaction(RT-PCR)analysis was *** was used to examine the activity of the nuclear factor-kappa B(NF-κB)signaling pathway and the production of TJs *** analyses were employed to localize the ***-linked immunosorbent assay(EliSA)and RT-PCR were utilized to observe changes in interleukin(IL)-1β*** investigate the role of NF-κB signaling activation and IL^(-1)βin Sema7A’s anti-barrier mechanism,we employed 0.1μmol/L IκB kinase 2(IKK2)inhibitor IV or 500 ng/mL IL^(-1)receptor(IL-1R)antagonist.●RESULTS:Treatment with Sema7A resulted in decreased TEER and increased permeability of Dextran-FITC in HCEs through down-regulating mRNA and protein levels of TJs in a time-and dose-dependent manner,as well as altering the localization of ***,Sema7A stimulated the activation of inhibitor of kappa B alpha(IκBα)and expression of IL-1β.The anti-barrier function of Sema7A was significantly suppressed by treatment with IKK2 inhibitor IV or IL-1R antagonists.●CONCLUSION:Sema7A disrupts barrier function through its influence on NF-κB-mediated expression of TJ proteins,as well as the expression of IL-1β.These findings suggest that Sema7A could be a potential therapeutic target for the diseases in corneal epithelium.

关键词： human corneal epithelial barrier function transepithelial electrical resistance zonula occludens-1 occludin nuclear factor-kappa B

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Pretreatment with interleukin-33 reduces warm hepatic ischemia/ reperfusion injury in mice

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Chinese Medical Journal 2013年第10期126卷 1855-1859页

作者： li Shu ZHU Feng-xue ZHANG Hong-bin li hui AN You-zhong Department of Critical Care Medicine Peking University People'sHospital Beijing 100044 China

Background Interleukin （IL）-33 is a recently identified member of the IL-1 family that binds to the receptor, ST2L. This study examined IL-33 production in mouse liver and investigated its role in hepatic ischemia/reperfusion （I/R） injury. Methods Male BALB/c mice （（22＋3） g） were subjected to 90 minutes partial hepatic ischemia, followed by 6 hours reperfusion. First, mice were randomized into two groups： control group （laparotomy only, without blocking blood supply） and ischemia model group. IL-33 mRNA and serum protein levels were measured at 30, 60, 90 minutes after ischemia and 2 and 6 hours after reperfusion. Second, mice were randomized into four groups： control, model （injection of rabbit IgG polyclonal antibody）, recombinant IL-33 intervention and anti-ST2L antibody intervention group. Mice were sacrificed 6 hours after reperfusion. liver pathology was observed via transmission electron microscopy. Serum aspartate aminotransferase （AST）, alanine aminotransferase （ALT）, IL-4, IL-5, IL-13, interferon-y （IFN-y） and tumor necrosis factor-a （TNF-a） levels were measured. Results Levels of IL-33 mRNA and protein did not change during ischemia （P 〉0.05） but increased significantly during reperfusion （P 〈0.05）. After reperfusion for 6 hours, serum levels of ALT, AST, IL-4, IL-5, IL-13, IFN-~ and TNF-a were significantly increased （P 〈0.05）, and hepatocellular ultrastructure was damaged. Pretreatment with IL-33 attenuated severity of liver damage compared with controls, but pretreatment with anti-ST2L antibody increased severity. Serum levels of IL-4, IL-5 and IL-13 protein increased whereas IFN-y decreased following IL-33 pretreatment. Pretreatment with anti-ST2L antibody significantly decreased serum IL-4, IL-5, IL-13 levels and increased serum IFN-r levels compared with controls （P 〈0.05）. There was no change in the level of TNF-a. Conclusion IL-33 is produced systematically and locally in liver during I/R injury. Pretreatment with IL-33 is therapeutic for hepatic

关键词： liver ischemia/reperfusion T helper lymphocyte,interleukin-33

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Reversal of Apoptotic Resistance by Lycium barbarum Glycopeptide 3 in Aged T Cells

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Biomedical and Environmental Sciences 2008年第3期21卷 212-217页

作者： LONG-GUO YUAN HONG-bin DENG li-hui CHEN DIAN-DONG li AND QI-YANG HE Institute of Medicinal BiotechnoIogy Peking Union Medical College and Chinese Academy of Medical Sciences Beijing 100050 China

Objective To study whether Lycium barbarurn glycopeplide 3 （LBGP3） affects T cell apoptosis in aged mice. Methods LBGP3 was purified with DEAE cellulose and Sephadex columns. Apoptotic ＂sub-G1 peak＂ was detected by flow cytometry and DNA ladder was resolved by agarose gel electrophoresis. Levels of IFN-7 and IL-10 were measured with specific kits and mRNA expression was detected by RT-PCR. Apoptosis-related proteins of FliP, FasL, and Bcl-2 were determined by Western blotting. Results LBGP3 was purified from Fructus Lycii water extracts and identified as a 41 kD glycopeptide. Treatment with 200 p.g/mL LBGP3 increased the apoptotic rate of T cells from aged mice and showed a similar DNA ladder pattern to that in young T cells. The reversal of apoptotic resistance was involved in down-regulating the expression of Bcl-2 and FliP, and up-regulating the expression of FasL. Conclusion Lycium barbarum glycopeptide 3 reverses apoptotic resistance of aged T cells by modulating the expression of apoptosis-related molecules.

关键词： Lycium barbarum glycopeptide 3 Aged T cells Cytokines Apoptosis Senescence

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基于PPCA修正模型的HRRP稳健识别方法

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西北工业大学学报 2016年第6期34卷 1022-1027页

作者：李彬李辉黄伟斌西北工业大学电子信息学院陕西西安710129

高信噪比情况下,利用概率主成分分析(PPCA,probabilistic principal component analysis)模型识别雷达高分辨距离像(HRRP,high resolution range profile)取得了较高的识别率。但在实际工作环境中,测试阶段获取的HRRP常为低信噪比样本,... 详细信息

高信噪比情况下,利用概率主成分分析(PPCA,probabilistic principal component analysis)模型识别雷达高分辨距离像(HRRP,high resolution range profile)取得了较高的识别率。但在实际工作环境中,测试阶段获取的HRRP常为低信噪比样本,由此造成的模型失配问题极大影响了识别性能。为此文章利用不同噪声来源造成的模型失配先验信息,在模型空间针对不同信噪比的测试样本补偿PPCA模型参数,以达到稳健识别的目的。另一方面,利用2种方法通过直接估计测试样本的噪声功率省去最优化计算的步骤,避免了求解最优补偿参数时需要大量计算的问题,提高了识别效率。最后,利用最大后验概率确定目标所属类别,证明了2种方法在信噪比低于20 d B时的可行性。

关键词：雷达目标识别飞机目标探测脉冲重复频率高分辨距离像概率主成分分析模型修正

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Preliminary study on road-region ecosystem stability evaluation

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Journal of Environmental Science and Engineering 2008年第5期2卷 48-54页

作者： QIN Zhi-bin liU Zhao-hui li Yu-zhi Changsha University of Science and Technology School of Highway Engineering Changsha 410076 China

A scientific and rational evaluation on road-region ecosystem stability is the premise to properly deal with the relationship between highway constructing and ecological environment protecting to a sustainable development. Based on the connotative meaning and the procedure of evaluation on road-region ecosystem stability, this paper studied the principles and the method on determining the evaluation index system on road-region ecosystem stability. It put forward an index system for assessing road-region ecosystem stability as a reference. On the basis of detailed analyze of the multidimensional space of road-region ecosystem, a new multi-objective comprehensive evaluation method for road-region ecosystem stability is presented and a calculation tormula for multi-objective comprehensive level of road-region ecosystem stability. This method was used to evaluate road-region ecosystem stability of lin-Chang highway. This method possessed definite theoretical value and reliability in practice.

关键词： road-region ecosystem evaluation of stability multi-objective multidimensional space

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Decoding the nexus:branched-chain amino acids and their connection with sleep,circadian rhythms,and cardiometabolic health

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Neural Regeneration Research 2025年第5期20卷 1350-1363页

作者： hui li Laurent Seugnet Department of Neurology Xijing HospitalXi’anShaanxi ProvinceChina Centre de Recherche en Neurosciences de Lyon Integrated Physiology of the Brain Arousal Systems(WAKING)UniversitéClaude Bernard Lyon 1INSERM U1028CNRS UMR 5292BronFrance

The sleep-wake cycle stands as an integrative process essential for sustaining optimal brain function and,either directly or indirectly,overall body health,encompassing metabolic and cardiovascular *** the heightened metabolic activity of the brain,there exists a considerable demand for nutrients in comparison to other *** these,the branched-chain amino acids,comprising leucine,isoleucine,and valine,display distinctive significance,from their contribution to protein structure to their involvement in overall metabolism,especially in cerebral *** the first amino acids that are released into circulation post-food intake,branched-chain amino acids assume a pivotal role in the regulation of protein synthesis,modulating insulin secretion and the amino acid sensing pathway of target of ***-chain amino acids are key players in influencing the brain's uptake of monoamine precursors,competing for a shared *** their involvement in protein synthesis,these amino acids contribute to the metabolic cycles ofγ-aminobutyric acid and glutamate,as well as energy ***,they impact GABAergic neurons and the excitation/inhibition *** rhythmicity of branchedchain amino acids in plasma concentrations,observed over a 24-hour cycle and conserved in rodent models,is under circadian clock *** mechanisms underlying those rhythms and the physiological consequences of their disruption are not fully *** sleep,obesity,diabetes,and cardiovascular diseases can elevate branched-chain amino acid concentrations or modify their oscillatory *** mechanisms driving these effects are currently the focal point of ongoing research efforts,since normalizing branched-chain amino acid levels has the ability to alleviate the severity of these *** this context,the Drosophila model,though underutilized,holds promise in shedding new light on these *** findings indicate its po

关键词： branched-chain amino acids cardiovascular health circadian clock drosophila insulin metabolism sleep γ-aminobutyric acid

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Resistance of Cement-based Grouting Materials with Nano- SiO_(2) Emulsion to Chloride Ion Penetration

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Journal of Wuhan University of Technology(Materials Science) 2025年第1期40卷 114-119页

作者： li Shuiping CHENG Jian WEI Chao YUAN bin YU Chengxiao College of Architectural Science and Engineering Yangzhou UniversityYangzhou 225127China

The chloride penetration resistance of cement-based grout materials was improved by nano-silica *** of mixtures containing different nano-silica particles or emulsions were exposed in sodium chloride solutions of specific concentrations with different test *** properties of the mixes were assessed in terms of weight loss and compressive strength.X-ray diffraction(XRD)and scanning electron microscopy(SEM)of mixes were performed to analysis the phase evolution and *** results demonstrated that the introduction of nano-SiO_(2) emulsion significantly decreased the compressive strength loss and calcium hydroxide(CH)crystal content of hydration production,and then enhanced the resistance of cement-based grouting materials to chloride ion *** improvement derives from the filling and pozzolanic effects of nano-SiO_(2) particles,which were incorporated via an emulsion and attributed to a well dispersion in grouting matrix.

关键词： grouting materials nano-SiO_(2)emulsion chloride ion penetration,weight loss strength loss

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Wave effect of gravitational waves intersected with a microlens field Ⅱ:An adaptive hierarchical tree algorithm and population study

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 241-254页

作者： Xikai Shan Guoliang li Xuechun Chen Wen Zhao bin Hu Shude Mao Department of Astronomy Tsinghua University Purple Mountain Observatory Chinese Academy of Sciences Institute for Frontier in Astronomy and Astrophysics Beijing Normal University School of Physics and Astronomy Beijing Normal University School of Astronomy and Space Science University of Science and Technology of China CAS Key Laboratory for Researches in Galaxies and Cosmology Department of Astronomy University of Science and Technology of ChinaChinese Academy of Sciences

The gravitational lensing wave effect generated by a microlensing field embedded in a lens galaxy is an inevitable phenomenon in strong lensed gravitational waves （SLGWs）.This effect presents both challenges and opportunities for the detection and application of ***,investigating this wave effect requires computing a complete diffraction integral over each microlens in the *** is extremely time-consuming due to the large number of microlenses （10～3-10～6）.Therefore,simply adding all the microlenses is ***,the complexity of the time delay surface makes the lens plane resolution a crucial factor in controlling numerical *** this paper,we propose a trapezoid approximation-based adaptive hierarchical tree algorithm to meet the challenges of calculation speed and *** find that this algorithm accelerates the calculation by four orders of magnitude compared to the simple adding method and is one order of magnitude faster than the fixed hierarchical tree algorithm proposed for electromagnetic *** importantly,our algorithm ensures controllable numerical errors,increasing confidence in the *** with our previous work （***.66,239511,2023）,this paper addresses all numerical issues,including integral convergence,precision,and computational time1).Finally,we conducted a population study on the microlensing wave effect of SLGWs using this algorithm and found that the microlensing wave effect cannot be ignored,especially for Type II SLGWs （from saddle position of the time delay surface） due to their intrinsic geometric structures and their typical intersection with a denser microlensing ***,more than 33%（11%） of SLGWs have a mismatch larger than 1%（3%） compared to the unlensed ***,we found that the mismatch between signal pairs in a doubly imaged GW is generally larger than 10-3,and 61%（25%） of signal pairs have a mismatch larger than 1%（3%）.Therefo

关键词： gravitational lensing diffractional integral algorithm gravitational wave

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Peripheral blood RNA biomarkers can predict lesion severity in degenerative cervical myelopathy

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Neural Regeneration Research 2025年第6期20卷 1764-1775页

作者： Zhenzhong Zheng Jialin Chen Jinghong Xu bin Jiang Lei li Yawei li Yuliang Dai bing Wang Department of Spine Surgery The Second Xiangya Hospital of Central South UniversityChangshaHunan ProvinceChina

Degenerative cervical myelopathy is a common cause of spinal cord injury,with longer symptom duration and higher myelopathy severity indicating a worse *** numerous studies have investigated serological biomarkers for acute spinal cord injury,few studies have explored such biomarkers for diagnosing degenerative cervical *** study involved 30 patients with degenerative cervical myelopathy(51.3±7.3 years old,12 women and 18 men),seven healthy controls(25.7±1.7 years old,one woman and six men),and nine patients with cervical spondylotic radiculopathy(51.9±8.6 years old,three women and six men).Analysis of blood samples from the three groups showed clear differences in transcriptomic *** analysis identified 128 differentially expressed genes that were enriched in patients with neurological *** least absolute shrinkage and selection operator analysis,we constructed a five-gene model(TBCD,TPM2,PNKD,EIF4G2,and AP5Z1)to diagnose degenerative cervical myelopathy with an accuracy of 93.5%.One-gene models(TCAP and SDHA)identified mild and severe degenerative cervical myelopathy with accuracies of 83.3%and 76.7%,*** of two immune cell types(memory B cells and memory-activated CD4^(+)T cells)predicted levels of lesions in degenerative cervical myelopathy with 80%*** results suggest that peripheral blood RNA biomarkers could be used to predict lesion severity in degenerative cervical myelopathy.

关键词： biomarkers candidate genes degenerative cervical myelopathy gene expression analysis immune cell types neurological disabilities peripheral blood RNA profiles spinal cord injury

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