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Chemical diversity and biological function of indolediketopiperazines from marine-derived fungi

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Marine Life Science & Technology 2020年第1期2卷 31-40页

作者： Jin Cao bin-gui wang Key Laboratory of Experimental Marine Biology Institute of OceanologyChinese Academy of Sciences and Laboratory of Marine Biology and BiotechnologyQingdao National Laboratory for Marine Science and TechnologyQingdao 266071China University of Chinese Academy of Sciences Beijing 100049China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China

Natural products from marine-derived fungi have attracted considerable attention in the recent two *** are one of the most important classes of marine natural products,mainly discovered from the fungal genera Penicillium,Aspergillus and *** compounds span a wide range of chemical structures and *** review summarizes 155 indolediketopiperazines that were discovered from marine-derived fungi from 2000 to early 2019 and primarily focuses on their chemical diversity and biological function.

关键词： Indolediketopiperazines Marine-derived fungi Natural products Chemical diversity Biological function

Mycochemistry of marine algicolous fungi

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Fungal Diversity 2016年第5期80卷 301-342页

作者： Nai-Yun Ji bin-gui wang Yantai Institute of Coastal Zone Research Chinese Academy of SciencesChunhui Road 17Yantai 264003China Laboratory of Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyKey Laboratory of Experimental Marine BiologyInstitute of OceanologyChinese Academy of SciencesNanhai Road 7Qingdao 266071China

Marine algicolous fungi refer to the fungi inhabiting or associated with marine algae,which have attracted a great attention for natural product researchers due to their chemical diversity and biological activity during the past two *** to the end of 2014,a total of 366 new natural products,including polyketides,terpenoids,polyketide-terpenoids,peptides,alkaloids,and shikimate derivatives,have been characterized from *** these metabolites,240 compounds feature cytotoxic,antibacterial,antifungal,antimicroalgal,zooplankton-toxic,antiprotozoal,antioxidative,enzyme-modulatory,and/or some other *** review gives a comprehensive coverage of both chemical and biological aspects of the secondary metabolites from marine algicolous fungi.

关键词： Marine algicolous fungus Endophyte Epiphyte Secondarymetabolite Bioactivity

Cloud point extraction coupled with HPLC-UV for the determination of phthalate esters in environmental water samples

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Journal of Environmental Sciences 2007年第7期19卷 874-878页

作者： wang Ling JIANG gui-bin CAI Ya-qi HE bin wang Ya-wei SHEN Da-zhong School of Chemistry and Chemical Engineering Shah Dong University Jinan 250100 China. State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences Chinese Academy of Scienees Beijing 100085 China

A method based on cloud point extraction was developed to determine phthalate esters including di-ethyl-phthalate （DEP）, di- （2-ethylhexyl）-phthalate （DEHP） and di-cyclohexyl-phthalate （DCP） in environmental water samples using high-performance liquid chromatography separation and ultraviolet detection （HPLC-UV）. The non-ionic surfactant Triton X-114 was chosen as extraction solvent. The parameters affecting extraction efficiency, such as concentrations of Triton X-114 and Na2SO4, equilibration temperature, equilibration time and centrifugation time were evaluated and optimized. Under the optimum conditions, the method can achieve preconcentration factors of 35, 88, 111 and detection of limits of 2.0, 3.8, 1.0 ng/ml for DEP, DEHP and DCP in 10-ml water sample, respectively. The proposed method was successfully applied to the determination of trace amount of phathalate esters in effluent water of the wastewater treatment plant and the lixivium of plastic fragments.

关键词： phthalate esters cloud point extraction Triton X-114 non-ionic surfactant, HPLC-UV

Prime Factorization in the Duality Computer

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Communications in Theoretical Physics 2007年第3期47卷 471-473页

作者： wang Wan-Ying SHANG bin wang Chuan LONG gui-Lu Key Laboratory for Quantum Information and Measurements and Department of Physics Tsinghua University Beijing 100084 China School of Computer Science ＆ Technology Beijing Institute of Technology Beijing 100081 China Key Laboratory of Atomic and Molecular Nanosciences Tsinghua University Beijing 100084 China

We give algorithms to factorize large integers in the duality computer. We provide three duality algorithms for factorization based on a naive factorization method, the Shor algorithm in quantum computing, and the Fer... 详细信息

关键词： duality computer prime factorization Fermat's method

Direct hydride derivatization of methyl- and ethylmercury chlorides in aqueous solution with KBH_4

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Journal of Environmental Sciences 2000年第3期12卷 275-278页

作者： HE bin JIANG gui-bin Research Centre for Eco Environmental Sciences Chinese Academy of SciencesBeijing 10085China

A convenient hydride derivatization procedure of methyl\|and ethylmercury chlorides to volatile hydrides was reported. In sealed vials methylmercury and ethylmercury compounds in acidic aqueous solutions were converted into their volatile forms by the reaction with potassium tetrahydroborate(KBH 4) and elvolved to the headspace of the vials. The gaseous analytes in the headspace were extracted and concentrated by solid phase microextraction(SPME) and injected into gas chromatography (GC) for separation and identified by mass selective detector(MS).

关键词： hydride derivatization methyl\| and ethylmercury gas chromatography mass selective detector

Evaluation of Stabilization Performances of Antioxidants in Poly(ether ether ketone)

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Chemical Research in Chinese Universities 2012年第5期28卷 907-911页

作者： ZHOU Zheng ZHOU Fu-gui ZHANG Shu-ling MU Jian-xin YUE Xi-gui wang gui-bin High Performance Plastics Engineering Research Center Ministry of EducationCollege of ChemistryJilin UniversityChangehun 130012P.R.China

Two types of antioxidants（a primary antioxidant and a secondary antioxidant） were used to improve the stability of poly（ether ether ketone）（PEEK）. The effects of the antioxidants on the properties of PEEK and the stabilization mechanism were investigated by some characterization methods, such as rheometer, thermogravimetric ana- lysis（TGA）, universal tester and electron spin resonance（ESR）. The results indicate that the efficiency of the phosphorous antioxidant（DS） in improving the stability of PEEK was better than that of the phenolic antioxidant（DN） in both melting stability and thermal stability, and the thermal stability of PEEK sample containing 0.07%（mass fraction） DS was the best among all the samples due to the decrease of the free radicals density, as proven by ESR measurement. Additionally, no obvious changes could be observed in mechanical properties of PEEK containing antioxidants compared to those of virgin PEEK.

关键词： Poly(ether ether ketone) Antioxidant Stability Mechanical property Radical

Involvement of glucose transporter 4 in ovarian development and reproductive maturation of Harmonia axyridis(Coleoptera:Coccinellidae)

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Insect Science 2022年第3期29卷 691-703页

作者： Yan Li Sha-Sha wang Su wang Shi-gui wang bin Tang Fang Liu College of Horticulture and Plant Protection Yangzhou UniversityYangzhouJiangsu225009China College of Life and Environmental Sciences Hangzhou Normal UniversityHangzhouZhejiang310036China Institute of Plant and Environment Protection Beijing Academy of Agricultural and Forestry SciencesBeijing100097China

Glucose is vital to embryogenesis,as are glucose *** transporter 4(Glut4)is one of the glucose transporters,which is involved in rapid uptake of glucose by various cells and promotes glucose *** energy metabolism in insect reproduction is well known,the molecular mechanism of Glut4 in insect reproduction is poorly *** suspect that Glut4 is involved in maintaining glucose concentrations in the ovaries and affecting vitellogenesis,which is critical for subsequent oocyte maturation and insect *** axyridis(Pallas)is a model organism for genetic research and a natural enemy of insect *** studied the influence of the Glut4 gene on the reproduction and development of *** using RNA interference *** transcription quantitative polymerase chain reaction analysis revealed that HaGlut4 was most highly expressed in *** of the HaGlut4 gene reduced the transcript levels of HaGlut4,and the weight and number of eggs produced significantly *** addition,the transcript levels of vitellogenin receptor and vitellogenin in the fat bodies and the ovaries of *** decreased after the interference of Glut4,and decreased the triglyceride,fatty acid,total amino acid and adenosine triphosphate content of *** resulted in severe blockage of ovary development and reduction of yolk formation;there was no development of ovarioles in the developing *** changes indicate that a lack of HaGlut4 can impair ovarian development and oocyte maturation and result in decreased fecundity.

关键词： glucose transporter 4(Glut4) Harmonia axyridis RNAi reproduction vitellogenin

Construction and characterization of calreticulin-HBsAg fusion gene recombinant adenovirus expression vector

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World Journal of Gastroenterology 2010年第24期16卷 3078-3082页

作者： Ma, Chun-Ling wang, gui-bin Gu, Run-Guo wang, Fang Shandong Med Coll Immunol Lab Linyi 276002 Shandong Peoples R China Renmin Hosp Linyi City Imaging Div Linyi 276003 Shandong Peoples R China Nanjing Med Univ Affiliated Hosp 1 Dept Lab Med Nanjing 210029 Jiangsu Prov Peoples R China

AIM: To generate recombinant adenoviral vector con-taining calreticulin (CRT)-hepatitis B surface antigen (HBsAg) fusion gene for developing a safe, effective and HBsAg-specific therapeutic ***: CRT and HBsAg gene were fused using polymerase chain reaction (PCR), endonuclease diges-tion and ligation methods. The fusion gene was cloned into pENTR/D-TOPO transfer vector after the base pairs of DNA (CACC) sequence was added to the 5′ end. Adenoviral expression vector containing CRT-HBsAg fusion gene was constructed by homologous recombinan-tion. The human embryo kidney (HEK) 293A cells were transfected with linearized DNA plasmid of the recombi-nant adenoviral vector to package and amplify recombi-nant adenovirus. The recombinant adenovirus titer was characterized using the end-dilution assay. The expres-sion of the CRT/HBsAg fusion protein in Ad-CRT/HBsAg infected 293A cells was detected by Western ***: The CRT-HBsAg fusion gene was char-acterized by PCR and sequencing and its length and sequence were confirmed to be accurate. The CRT-HB-sAg fusion gene recombinant pENTR/D-TOPO transfer vector was constructed. The recombinant adenoviral vector, Ad-CRT/HBsAg, was generated successfully. The titer of Ad-CRT/HBsAg was characterized as 3.9 × 1011 pfu/mL. The CRT-HBsAg fusion protein was ex-pressed by HEK 293A cells correctly. CONCLUSION: CRT/HBsAg fusion gene recombinant replication-defective adenovirus expression vector is constructed successfully and this study has provided an experimental basis for further studies of Hepatitis B vi-rus gene therapy.

关键词： Calreticulin Hepatitis B virus Hepatitis B surface antigen Adenovirus expression vector Fusion protein Therapeutic vaccine

Functional characterization of sex pheromone receptors in Spodoptera frugiperda, S. exigua, and S. litura moths

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Insect Science 2023年第2期30卷 305-320页

作者： Sai Zhang Fang Liu bin Yang Yang Liu gui-Rong wang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBejingChina College of Horticulture and Plant Protection Yangzhou UniversityYangzhouChina Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenChina

Moths possess an extremely sensitive and diverse sex pheromone processing system,in which pheromone receptors(PRs)are essential to ensure communication between mating *** properties of some PRs are conserved among species,which is important for ***,functional differentiation has occurred in some homologous PR genes,which may drive species ***,using genome analysis,17 PR genes were identified from Spodoptera frugiperda,***,and ***,which belong to 6 homologous groups(odorant receptor[OR]6,11,13,16,56,and 62);of which 6 PR genes(OR6,OR11,OR13,OR16,OR56,and OR62)were identified in *** and ***,and 5 PR genes were identified in ***,excluding *** heterologous expression in Xenopus oocytes,we characterized the functions of PR orthologs including OR6,OR56,and OR62,which have not been clarified in previous ***6 orthologs were specifically tuned to(Z,E)-9,12-tetradecadienyl acetate(Z9,E12-14:OAc),and OR62 orthologs were robustly tuned to Z7-12:OAc in *** and *** optimal ligand for OR56 was Z7-12:OAc in ***,but responses were minimal in *** and *** addition,SfruOR6 was male antennae-specific,whereas SfruOR56 and SfruOR62 were male *** study further clarified the functional properties of PRs in 3 Spodoptera moth species,providing a comprehensive understanding of the mechanisms of intraspecific communication and interspecific isolation in Spodoptera.

关键词： pheromone pheromone receptor Spodoptera Xenopus oocytes