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A new naphthoquinoneimine derivative from the marine algal-derived endophytic fungus Aspergillus niger EN-13

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Chinese Chemical Letters 2007年第8期18卷 951-953页

作者： Yi Zhang Xiao Ming Li Chang Yun Wang bin gui Wang Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China Key Laboratory of Marine Drugs Ministry of Education Institute of Marine Drugs Ocean University of China Qingdao 266003 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China

Cultivation of an endophytic fungus Aspergillus niger EN-13 that was isolated from the inner tissue of the marine brown alga Colpomenia sinuosa resulted in the characterization of a new naphthoquinoneimine derivative, namely, 5,7-dihydroxy-2-[1-（4- methoxy-6-oxo-6H-pyran-2-yl）-2-phenylethylamino]-[ 1,4]naphthoquinone. The structure of the new compound was established on the basis of various NMR spectroscopic analyses including 2D NMR techniques, El-MS, and HR-ESI-MS. This compound displayed moderate antifungal activity.

关键词： Colpomenia sinuosa Endophytic fungus Aspergillus niger Naphthoquinoneimine 5,7-Dihydroxy-2-[1-(4-methoxy-6-oxo-6H-pyran-2-yl)-2-phenylethylamino]-[ 1,4]naphthoquinone

Construction and characterization of calreticulin-HBsAg fusion gene recombinant adenovirus expression vector

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World Journal of Gastroenterology 2010年第24期16卷 3078-3082页

作者： Ma, Chun-Ling Wang, gui-bin Gu, Run-Guo Wang, Fang Shandong Med Coll Immunol Lab Linyi 276002 Shandong Peoples R China Renmin Hosp Linyi City Imaging Div Linyi 276003 Shandong Peoples R China Nanjing Med Univ Affiliated Hosp 1 Dept Lab Med Nanjing 210029 Jiangsu Prov Peoples R China

AIM: To generate recombinant adenoviral vector con-taining calreticulin (CRT)-hepatitis B surface antigen (HBsAg) fusion gene for developing a safe, effective and HBsAg-specific therapeutic ***: CRT and HBsAg gene were fused using polymerase chain reaction (PCR), endonuclease diges-tion and ligation methods. The fusion gene was cloned into pENTR/D-TOPO transfer vector after the base pairs of DNA (CACC) sequence was added to the 5′ end. Adenoviral expression vector containing CRT-HBsAg fusion gene was constructed by homologous recombinan-tion. The human embryo kidney (HEK) 293A cells were transfected with linearized DNA plasmid of the recombi-nant adenoviral vector to package and amplify recombi-nant adenovirus. The recombinant adenovirus titer was characterized using the end-dilution assay. The expres-sion of the CRT/HBsAg fusion protein in Ad-CRT/HBsAg infected 293A cells was detected by Western ***: The CRT-HBsAg fusion gene was char-acterized by PCR and sequencing and its length and sequence were confirmed to be accurate. The CRT-HB-sAg fusion gene recombinant pENTR/D-TOPO transfer vector was constructed. The recombinant adenoviral vector, Ad-CRT/HBsAg, was generated successfully. The titer of Ad-CRT/HBsAg was characterized as 3.9 × 1011 pfu/mL. The CRT-HBsAg fusion protein was ex-pressed by HEK 293A cells correctly. CONCLUSION: CRT/HBsAg fusion gene recombinant replication-defective adenovirus expression vector is constructed successfully and this study has provided an experimental basis for further studies of Hepatitis B vi-rus gene therapy.

关键词： Calreticulin Hepatitis B virus Hepatitis B surface antigen Adenovirus expression vector Fusion protein Therapeutic vaccine

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双眼分视治疗弱视的进展

中国斜视与小儿眼科杂志 2024年第1期32卷 46-48+31页

作者：罗桂彬刘涵方晏红张蓝月重庆大学附属江津医院眼科

弱视除了影响视力,还影响双眼视觉,传统的弱视治疗往往只关注弱视眼的视力恢复而忽略了更高阶的视觉恢复。研究发现,双目闪烁眼镜和虚拟现实、双眼视频游戏、双眼显示屏等双眼治疗不仅可以提升弱视眼视力,还可以提升高阶视功能,且患者... 详细信息

弱视除了影响视力,还影响双眼视觉,传统的弱视治疗往往只关注弱视眼的视力恢复而忽略了更高阶的视觉恢复。研究发现,双目闪烁眼镜和虚拟现实、双眼视频游戏、双眼显示屏等双眼治疗不仅可以提升弱视眼视力,还可以提升高阶视功能,且患者依从性更好,对大龄儿童、成年弱视也有一定的效果。探索双眼分视治疗弱视,为弱视治疗提供新的方向。

关键词：弱视双眼分视治疗闪烁眼镜虚拟现实双眼视频游戏双眼显示屏

Determination of alkali-labile phosphoprotein phosphorus from fish plasma using the Tb^(3+)-tiron complex as a fluorescence probe

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Journal of Environmental Sciences 2007年第5期19卷 616-621页

作者： LV Xue-fei ZHAO Yi-bing ZHOU Qun-fang JIANG gui-bin SONG Mao-yong State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beifing 100085 China Department of Chemistry the Key Laboratory of Analytical Sciences of Ministry of Education Xiamen University Xiamen 361005 China

A sensitive method based on the fluorescence quenching effect of the Tb^3＋-Tiron complex is proposed for the determination of alkali-labile phosphoprotein phosphorus （ALP） released from fish plasma. The detection limit was 5.4 ng/ml （S/N=2）, and the relative standard deviation of the quenching effect （6 replicates） was 4.6%. The results obtained by the proposed method were in good agreement with those obtained by the colorimetric assay. The advantages of the present method are its relatively simple detection procedure, the lack of toxic organic solvents, and high sensitivity.

关键词： alkali-labile phosphoprotein phosphorus (ALP) Chinese loach fluorescence quenching Tb^3+-Tiron complex vitellogenin

Development and Evaluation of a 7-DOF Haptic Interface

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IEEE/CAA Journal of Automatica Sinica 2018年第1期5卷 261-269页

作者： Jian-Long Hao Xiao-Liang Xie gui-bin Bian Zeng-Guang Hou Xiao-Hu Zhou Key Laboratory of Management and Control for Complex Systems Institute of Automation University of Chinese Academy of Sciences Beijing 100190 China IEEE

With the development of human robot interaction technologies, haptic interfaces are widely used for 3 D applications to provide the sense of touch. These interfaces have been utilized in medical simulation, virtual assembly and remote manipulation tasks. However, haptic interface design and control are still critical problems to reproduce the highly sensitive touch sense of humans. This paper presents the development and evaluation of a7-DOF(degree of freedom) haptic interface based on the modified delta mechanism. Firstly, both kinematics and dynamics of the modified mechanism are analyzed and presented. A novel gravity compensation algorithm based on the physical model is proposed and validated in simulation. A haptic controller is proposed based on the forward kinematics and the gravity compensation algorithm. To evaluate the control performance of the haptic interface, a prototype has been implemented. Three kinds of experiments: gravity compensation, static response and force tracking are performed respectively. The experimental results show that the mean error of the gravity compensation is less than 0.7 N and the maximum continuous force along the axis can be up to 6 N. This demonstrates the good performance of the proposed haptic interface.

关键词： Dynamic modeling evaluation haptic interface impedance control

Two new brominated diterpenes from Laurencia decumbens

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Chinese Chemical Letters 2007年第8期18卷 957-959页

作者： Nai Yun Ji Xiao Ming Li Chuan Ming Cui bin gui Wang Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China Graduate School of the Chinese Academy of Sciences Beijing 100049 China

Two new brominated diterpenes, namely, laurendecumtriol and 11-deacetylpinnaterpene C, were isolated and identified from the marine red alga Laurencia decumbens. Their structures were established on the basis of vario... 详细信息

关键词： Laurencia decumbens Diterpene Laurendecumtriol 11-Deacetylpinnaterpene C

Gridded inventories of historical usage for selected organochlorine pesticides in Heilongjiang River Basin, China

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Journal of Environmental Sciences 2006年第4期18卷 822-826页

作者： LIU Li-yan JIANG An-xi REN Nan-qi JIANG gui-bin LI Yi-fan School of Municipal and Environmental Engineering ＇Harbin Institute of Technology Harhin 150090 China Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China Atmospheric Environment Service Environment Canada Downsview Ontario Canada

The use of technical HCH （1, 2, 3, 4, 5, 6-hexachlorocyclohexane）, DDT （dichlorodiphenyltrichloroethane） and lindane in Heilongjiang River Basin （HRB） of China was studied. Between 1952 and 1984 the total usage in the HRB was 108900 t for technical HCH, and 4900 t for DDT, respectively, and the use of DDT due to dicofol application in the HRB was 220 t from 1984 to 2003. The usage of lindane in HRB was 109 t from 1991 to 2000. The results show that the highest technical HCH use （41800 t） in HRB was on maize, accounting for 38.3% of the total usage on all crops, followed by the use on wheat （28000 t, 25.7%） and on soybean （26600 t, 24.4%）. The highest DDT use （2300 t） was on soybean, accounting for 46.8% of the total usage on all crops, followed by the use on maize （1500 t, 31.3%） and on sorghum （600 t, 13.2%）. The major use of lindane was mainly on wheat. Gridded usage inventories of these three OCPs （organochlorine pesticides）, in HRB with a 1/6° latitude by 1/4° longitude resolution have been created by using different gridded cropland as surrogates. Some soil samples have also been collected in HRB. Data of usage and soil concentrations of 5： HCH in HRB match quite well to those in Shanghai region, and much higher soil concentration of 5： DDT in Shanghai region could be due to much heavier dicofol use in this region.

关键词： persistent organic pollutants (POPs) organochlorine pesticides (OCPs) DDT HCH lindane inventories Heilongjiang River Basin

Two new aristolane sesquiterpenes from Laurencia similis

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Chinese Chemical Letters 2007年第2期18卷 178-180页

作者： Nai Yun Ji Xiao Ming Li Lan Ping Ding bin gui Wang Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China Graduate School of the Chinese Academy of Sciences Beijing 100049 China

Two new aristolane sesquiterpenes, namely, aristolan-10-ol-9-one and aristolan-8-en-1-one, were isolated from the red alga Laurencia similis. Their structures were established on the basis of various NMR spectroscopic... 详细信息

关键词： Laurencia similis Aristolane Aristolan-10-ol-9-one Aristolan-8-en-1-one

A Novel Hydroperoxyl Substituted Cembranolide Diterpene from Marine Soft Coral Lobophytum crassum

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Chinese Chemical Letters 2005年第11期16卷 1489-1491页

作者： Shuai Wen YIN Yan Ping SHI Xiao Ming LI bin gui WANG Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 Key Laboratory for Natural Medicine of Gansu Province Lanzhou Institute of Chemical Physics Chinese Academy of Sciences Lanzhou 730000 Graduate School of the Chinese Academy of Sciences Beijing 100039

A new cembranolide diterpene with a hydroperoxyl substitution was isolated from the marine soft coral Lobophytum crassum. The structure was elucidated on the basis of chemical and spectral methods.

关键词： Lobophytum crassum 2-hydroperoxysarcophine cembranolide diterpene.