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Co-overexpression of genes for nitrogen transport, assimilation, and utilization boosts rice grain yield and nitrogen use efficiency

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The Crop Journal 2023年第3期11卷 785-799页

作者： Jie Luo Junnan Hang bilong wu Xilin Wei Quanzhi Zhao Zhongming Fang Institute of Rice Industry Technology Research Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education)College of Agricultural SciencesGuizhou UniversityGuiyang 550025GuizhouChina Center of Applied Biotechnology Wuhan University of BioengineeringWuhan 430415HubeiChina

Nitrogen(N)fertilization is necessary for obtaining high rice *** excessive N fertilizer reduces rice plant N efficiency and causes negative effects such as environmental *** this study,we assembled key genes involved in different nodes of N pathways to boost nitrate and ammonium uptake and assimilation,and to strengthen amino acid utilization to increase grain yield and nitrogen use efficiency(NUE)in *** combinations OsNPF8.9a×OsNR2,OsAMT1;2×OsGS1;2×OsAS1,and OsGS2×OsAS2×OsANT3 optimized nitrate assimilation,ammonium conversion,and N reutilization,*** co-overexpressing rice lines obtained by co-transformation,the tiller number,biomass,and grain yield per plant of the OsAMT1;2×OsGS1;2×OsAS1-overexpressing line exceeded those of wild-type ZH11,the OsNPF8.9a×OsNR2×OsGS1;2×OsAS1-overexpressing line,and the OsGS2×OsAS2×OsANT3-overexpressing *** glutamine synthase activity,free amino acids,and nitrogen utilization efficiency(NUt E)of the OsAMT1;2×OsGS1;2×OsAS1-overexpressing line exceeded those of ZH11 and other lines that combined key genes.N influx efficiency was increased in the OsAMT1;2×OsGS1;2×OsAS1-overexpressing line and OsNPF8.9a×OsNR2×OsGS1;2×OsAS1-overexpressing line under a low ammonium and a low nitrate treatment,*** propose that combining overexpression of OsAMT1;2,OsGS1;2,and OsAS1 is a promising breeding strategy for systematically increasing rice grain yield and NUE by focusing on key nodes in the N pathway.

关键词： Nitrogen Rice tillering Grain yield Nitrogen use efficiency Co-transformation

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雅万高铁引入雅加达枢纽方案研究

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铁道工程学报 2022年第6期39卷 4-10页

作者：吴必龙中国铁路设计集团有限公司天津300308

研究目的:通过分析雅加达铁路枢纽现状布局,结合雅万高铁功能定位,从客流吸引能力、工程建设条件、与其他轨道交通方式换乘条件、市政配套设施条件、城市总体规划等方面,采用权重分析法确定雅万高铁引入雅加达枢纽的站址选择方案,并针... 详细信息

研究目的:通过分析雅加达铁路枢纽现状布局,结合雅万高铁功能定位,从客流吸引能力、工程建设条件、与其他轨道交通方式换乘条件、市政配套设施条件、城市总体规划等方面,采用权重分析法确定雅万高铁引入雅加达枢纽的站址选择方案,并针对哈利姆站三台六线规模,通过时分计算和图解分析分别研究非高峰和高峰时段的车站作业能力。研究结论:(1)雅万高铁引入雅加达枢纽,近期新建哈利姆站,预留远期延伸至甘比尔站、芒加莱站条件,该方案既能满足近、远期客运需求,又契合城市重点发展方向,近期投资省,建设难度小,也充分预留了远期发展条件;(2)本研究成果可为后续海外类似铁路项目设计提供借鉴和参考。

关键词：雅万高铁哈利姆站站址选择车站规模

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借鉴德国,思考我国节能建筑设计

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工业建筑 2006年第9期36卷 104-105页

作者：吴必龙李颖浙江科技学院杭州310012 中国美术学院综合设计系杭州310007

节约能源和保护环境是德国政府开发利用能源的一贯政策。德国特别重视建筑的节能,并收到了很好的效果,很多经验值得我们借鉴。从建筑节能设计角度分析,认为我国在建筑节能设计时,不应该简单复制节能建筑,应当变革现有的设计模式,从建筑... 详细信息

节约能源和保护环境是德国政府开发利用能源的一贯政策。德国特别重视建筑的节能,并收到了很好的效果,很多经验值得我们借鉴。从建筑节能设计角度分析,认为我国在建筑节能设计时,不应该简单复制节能建筑,应当变革现有的设计模式,从建筑的寿命周期着眼,系统运用高新技术,使建筑技术和建筑艺术相融合,并得到政府的支持。

关键词：节能建筑建筑设计设计模式设计理念

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铁路客运站候车区运用方案优化方法和模型

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交通信息与安全 2013年第4期31卷 53-56页

作者：吴必龙刘作义北京交通大学交通运输学院北京100044

候车区是铁路客运站内旅客聚集密度最大、停留时间最长的区域,其合理运用对提高客运站的运输组织效率有着重要的作用。文章对铁路客运站候车区车次分配问题建立了基于三目标的数学优化模型(优化的3个目标分别是:一个候车区在同一时间只... 详细信息

候车区是铁路客运站内旅客聚集密度最大、停留时间最长的区域,其合理运用对提高客运站的运输组织效率有着重要的作用。文章对铁路客运站候车区车次分配问题建立了基于三目标的数学优化模型(优化的3个目标分别是:一个候车区在同一时间只能被一个车次占用、便于旅客进站上车、保持候车室运用和负荷均衡性),并对该模型进行算法设计和求解。通过实例验证和参数微调对比,对候车室运用方案的优劣进行了评价,验证了数学模型和算法的正确性。

关键词：铁路客运站候车区运用方案

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Co-overexpression of genes for nitrogen transport, assimilation, and utilization boosts rice grain yield and nitrogen use efficiency

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The Crop Journal 2023年第3期

作者： Jie Luo Junnan Hang bilong wu Xilin Wei Quanzhi Zhao Zhongming Fang

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对多普勒效应演示实验的建议

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物理教学探讨 2008年第8期26卷 47-47页

作者：吴必龙丹阳高级中学江苏省丹阳市212309

高中物理选修3-4《多普勒效应》一节中,课文用演示实验《蜂鸣器音调的变化》引入新课,为学生提供了直观的生活实例,使学生能迅速准确地理解多普勒效应作好铺垫,确实是一个好实笔验。者做了这个实验,学生感觉到声音的变化

关键词：演示实验蜂鸣器圆周运动多普勒效应波源频率

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Sex-dependent alterations in extracellular vesicles linking chronic spinal cord injury to brain neuroinflammation and neurodegeneration

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Neural Regeneration Research 2025年第2期20卷 483-484页

作者： Yun Li Junfang wu Department of Anesthesiology&Shock Trauma and Anesthesiology Research(STAR)CenterUniversity of Maryland School of MedicineBaltimoreMDUSA

Traumatic spinal cord injury(SCI)is a devastating exogenous injury with long-lasting consequences and a leading cause of death and disability *** in assistive technology,rehabilitative interventions,and the ability to identify and intervene in secondary conditions have significantly increased the long-term survival rate of SCI patients,with some people even living well into their seventh or eighth *** survival changes have led neurotrauma researchers to examine how SCI interacts with brain *** health and epidemiological data showed that patients with long-term SCI can have a lower life expectancy and quality of life,along with a higher risk of comorbidities and complications.

关键词： alterations inflammation injury

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Gut microbiota shifts in hepatitis B-related portal hypertension after transjugular intrahepatic portosystemic shunt:Mechanistic and clinical implications

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World Journal of Gastroenterology 2025年第3期31卷 134-137页

作者： Qi-Rong Jiang Da-wu Zeng Department of Hepatology Hepatology Research InstituteThe First Affiliated HospitalFujian Medical UniversityFuzhou 350005Fujian ProvinceChina Department of Infectious Diseases The First Affiliated Hospital of Fujian Medical UniversityFuzhou 350004Fujian ProvinceChina

In this article,we provide commentary on the recent article by Zhao et *** focus on the shifts in the gut microbiota of patients with hepatitis B virus(HBV)-associated cirrhosis/portal hypertension(PH)following transjugular intrahepatic portosystemic shunt(TIPS)and the implications for understanding the mechanisms,diagnosis,and *** comparing the gut microbiota composition and dynamic changes before and after TIPS in patients with and without hepatic encephalopathy,the authors found an increase in non-probiotic bacteria in those who developed hepatic encephalopathy post-TIPS,with Morganella species present only in the hepatic encephalopathy *** gut microbiota changes post-TIPS among patients without the occurrence of hepatic encephalopathy suggest potential therapeutic benefits through prophylactic microbiome ***,the specific gut microbiota alterations may hold promise to predict the risk of hepatic encephalopathy in individuals undergoing TIPS for HBVrelated *** these promising findings,future studies are needed to address limitations,including a small sample size,a relatively short evaluation period for gut microbiota alterations,the absence of data on dynamic alterations in gut microbiota post-TIPS and their correlation with blood ammonia levels,and the lack of validation in animal *** conclusion,Zhao et al's study has shed new light on the link of gut microbiota with post-TIPS hepatic encephalopathy,potentially through the intricate gut-liver axis,and has important clinical implications for improving the management of patients with HBV-related PH.

关键词： Hepatitis B virus Cirrhosis Portal hypertension Hepatic encephalopathy Transjugular intrahepatic portosystemic shunt Gut microbiota

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Retrospective analysis of pathological types and imaging features in pancreatic cancer: A comprehensive study

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World Journal of Gastrointestinal Oncology 2025年第1期17卷 121-129页

作者： Yang-Gang Luo Mei wu Hong-Guang Chen Pathology Department Xuanhan County People’s HospitalDazhou 636150Sichuan ProvinceChina

BACKGROUND Pancreatic cancer remains one of the most lethal malignancies worldwide,with a poor prognosis often attributed to late *** the correlation between pathological type and imaging features is crucial for early detection and appropriate treatment *** To retrospectively analyze the relationship between different pathological types of pancreatic cancer and their corresponding imaging *** We retrospectively analyzed the data of 500 patients diagnosed with pancreatic cancer between January 2010 and December 2020 at our *** types were determined by histopathological examination of the surgical spe-cimens or biopsy *** imaging features were assessed using computed tomography,magnetic resonance imaging,and endoscopic *** analyses were performed to identify significant associations between pathological types and specific imaging *** There were 320(64%)cases of pancreatic ductal adenocarcinoma,75(15%)of intraductal papillary mucinous neoplasms,50(10%)of neuroendocrine tumors,and 55(11%)of other rare *** imaging features were identified in each pathological *** ductal adenocarcinoma typically presents as a hypodense mass with poorly defined borders on computed tomography,whereas intraductal papillary mucinous neoplasms present as characteristic cystic lesions with mural *** tumors often appear as hypervascular lesions in contrast-enhanced *** analysis revealed significant correlations between specific imaging features and pathological types(P<0.001).CONCLUSION This study demonstrated a strong association between the pathological types of pancreatic cancer and imaging *** findings can enhance the accuracy of noninvasive diagnosis and guide personalized treatment approaches.

关键词： Pancreatic cancer Pathological types Imaging features Retrospective analysis Diagnostic accuracy

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Characteristics of gut microbiota dysbiosis in patients with colorectal polyps

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World Journal of Gastrointestinal Oncology 2025年第1期17卷 13-21页

作者： Xian-Rong wu Xiao-Hong He Yong-Fang Xie School of Life Health Information Science and Engineering Chongqing Post and Communications UniversityChongqing 400065China

This editorial,inspired by a recent study published in the World Journal of Gastrointestinal Oncology,covers the research findings on microbiota changes in various *** recurrent colorectal polyps,the abundances of Klebsiella,Parvimonas,and Clostridium increase,while those of Bifidobacterium and Lactoba-cillus *** dysbiosis may promote the formation and recurrence of *** microbial changes have also been observed in colorectal cancer,inflammatory bowel disease,autism spectrum disorder,and metabolic syndrome,indicating the role of increased pathogens and decreased probiotics in these *** the gut microbiota,particularly by increasing probiotic levels,may help prevent polyp recurrence and promote gut *** microbial intervention strategy holds promise as an adjunctive treatment for patients with colorectal polyps.

关键词： Recurrent colorectal polyps Gut microbiota dysbiosis Klebsiella Probiotics Intestinal inflammation Microbial intervention strategy

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