检索结果-南通市图书馆

Hypobaric-hypoxia Induces Alteration in Microbes and Microbes-associated Enzyme Profile in rat Colonic Samples

Biomedical and Environmental Sciences 2013年第10期26卷 869-873页

作者： Chiranjit MAITY Atanu ADAK Kuntal GHOSH bikas r pati Keshab C MONDAL Department of Microbiology Vidyasagar University Special Center for Molecular Medicine Jawaharlal Nehru University

Present study deals with the straight impact of hypobaric hypoxia on the quantity and composition of some predominant fecal microflora and its functional aspects. For that, isolated fecal contents of rat were exposed to two different simulated air pressures （70 kPa and 40 kPa） for different time durations （1, 3, and 5 h） and the bacterial community composition was compared with normobaric groups （101.3 kPa）. It was found that the total anaerobes, Escherichio coli, Enterbocters spp., Bi^idobocterium spp., CIostridium spp. were increased whereas total aerobes were decreased at both hypobaric treatments. The increased number of amplicon was detected in the pressure-treated groups than the control that clearly mentioned the disruption of microbiota structure at different simulated hypobaric-hypoxia. The amylase, protease, tannase, 13-glucuronidase, and alkaline phosphatase activities were increased at these atmospheric pressures. Thus, the present investigation demonstrates that the hypobaric hypoxia is an important environmental factor which can strongly modulate the composition of intestinal flora as well as microfiora-derived functional aspects.

关键词： Hypobaric-hypoxia Induces Alteration in Microbes and Microbes-associated Enzyme Profile in rat Colonic Samples

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Molecular exposition of broad‑spectrum antibacterial efficacy by p‑coumaric acid from an edible mushroom Termitomyces heimii:in vitro and in silico approach

引用

Systems Microbiology and Biomanufacturing 2023年第4期3卷 750-764页

作者： Krishanu Singha Amrita Banerjee Arijit Jana Poushali Bandyopadhyay Smarajit Maiti bikas ranjan pati Pradeep Kumar Das Mohapatra Department of Microbiology Vidyasagar UniversityMidnaporeWest Bengal 721102India Department of Biotechnology Oriental Institute of Science and TechnologyMidnaporeWest Bengal 721102India Department of Chemical Engineering IIT RoorkeeUttarakhand 247667India Department of Microbiology Raiganj UniversityRaiganjWest Bengal 733134India

P-Coumaric acid was previously reported to contain antioxidant,antidiabetic,anti-inflammatory,antiplatelet,antiulcer and anticancer *** with these,the present work has been conducted to study the antibacterial activity of p-coumaric *** could be used to control broad-spectrum microbiome-based inflammation or in cancer *** analysis of methanolic extract from the mushroom Termitomyces heimii has exhibited a rich fraction of p-coumaric acid(p-CA),which in the pure form showed significant bactericidal *** predict the molecular interactions associated with the bactericidal mechanism of p-CA,the transmembrane protein sequences of Staphylococcus aureus and Escherichia coli were retrieved from the IMG–JGI database,screened and then aligned using Clustal X2 and PHYLIP 3.69 *** common sequences were subjected to tertiary structure prediction using Phyre2 server,followed by quality assessment through the ramachandran ***,the 3D molecular structure of p-CA was downloaded from PubChem and docked with the selected tertiary structures using Patchdock and showed higher affinity towards 12 common transmembrane protein structures,amongst which CDP-diacylglycerol–glycerol-3-phosphate 3-phosphatidyl transferase(PgsA)exhibited best dock-ing with p-CA on the basis of ACE value(–249 kcal/mol).This fact revealed that p-CA can block the normal functioning of membrane-bound enzyme PgsA,consequently leading to the interruption in the synthesis and recycling of an essential membrane component phosphatidylglycerol(PG),resulting in membrane disruption followed by cell ***,for the first time we reported the molecular insights and fundamental biochemical events underlying the bactericidal action by p-CA,to explore new perspective to combat multidrug-resistant bacteria.

关键词： p-Coumaric acid Bactericidal Docking ACE value PgsA Lysis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：