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Fluorescent nanoswitch for monitoring specific pluripotency-related microRnas of induced pluripotent stem cells： Development of polyethyleneimine- oligonucleotide hybridization probes

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nano Research 2017年第8期10卷 2545-2559页

作者： Seungmin Han Hye Young Son Byunghoon Kang Eunji Jang Jisun Ki na Geum Lee Jongjin Park Moo-Kwang shin Byeonggeol Mun Jeong-Ki Min Seungjoo Haam Department of Chemical and Biomolecular Engineering College of Engineering Yonsei University Seou1120-749 Republic of Korea Department of Radiology College of Medicine Yonsei University Seou1120-752 Republic of Korea Severance Biomedical Science Institute College of Medicine Yonsei University Seou1120-752 Republic of Korea Biotherapeutics Translational Research Center Korea Research Institute of Bioscience and Biotechnology Daejeon 34141 Republic of Korea Department of Biomolecular Science University of Science & Technology Daejeon 34113 Republic of Korea

The isolation of high-grade （i.e. high-pluripotency） human induced pluripotent stem cells （hiPSCs） is a decisive factor for enhancing the purity of hiPSC populations or differentiation efficiency. A non-invasive imaging system that can monitor microRna （miRna） expression provides a useful tool to identify and analyze specific cell populations. However, previous studies on the monitoring/isolation of hiPSCs by miRna expression have limited hiPSCs＇ differentiation system owing to long-term incubation with miRna imaging probe-nanocarriers. Therefore, we focused on monitoring high-grade hiPSCs without influencing the pluripotency of hiPSCs. We reduced nanoparticle transfection time, because hiPSCs are prone to spontaneous differentiation under external factors during incubation. The fluorescent nanoswitch （＂ON＂ with target miRna）, which can be applied for either imaging or sorting specific cells by fluorescence signals, contains an miRna imaging probe （miP） and a PEI-PEG nanoparticle （miP-P）. Consequently, this nanoswitch can sense various endogenous target miRnas within 30 min in vitro, and demonstrates strong potential for not only imaging but also sorting pluripotent hiPSCs without affecting pluripotency. Moreover, miP-P-treated hiPSCs differentiate well into endothelial cells, indicating that miP-P does not alter the pluripotency of hiPSCs. We envisage that this miRna imaging system could be valuable for identifying and sorting high-grade hiPSCs for improved practical applications.

关键词： induced pluripotent stem cells (iPSCs) pluripotency microRna (miRna) fluorescence imaging nanoparticle

采用等通道转角挤压通过晶粒细化同步提高Mg-Zn-Ca合金的耐腐蚀性能和力学性能(英文)

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Transactions of Nonferrous Metals Society of China 2024年

作者：王艺卓罗小萍任广笑王红霞王利飞程伟丽李航鲁小鹏 shin Kwang-seon 太原理工大学材料科学与工程学院太原科技大学材料科学与工程学院山西江淮重工有限责任公司东北大学材料科学与工程学院 Magnesium Technology Innovation Center， School of Materials Science and Engineering， Seoul national University， Gwanak-ro

为了研究显微组织演变对Mg-1Zn-1Ca(wt%)合金的腐蚀行为和强化机制的影响，对铸态Mg-1Zn-1Ca合金分别进行了ECAP一道次和四道次挤压。通过光学显微镜（OM）、通过扫描电子显微镜（SEM）、电子背散射衍射（EBSD）、电化学试验、浸泡试验... 详细信息

为了研究显微组织演变对Mg-1Zn-1Ca(wt%)合金的腐蚀行为和强化机制的影响，对铸态Mg-1Zn-1Ca合金分别进行了ECAP一道次和四道次挤压。通过光学显微镜（OM）、通过扫描电子显微镜（SEM）、电子背散射衍射（EBSD）、电化学试验、浸泡试验和拉伸试验研究了合金的腐蚀行为和力学性能。结果表明，ECAP一道次挤压后，力学性能有所改善，耐腐蚀性能有所下降，这由于ECAP挤压产生了高密度位错和破碎的第二相。相比之下，合金经过ECAP四道次挤压后通过细化晶粒实现了力学性能和耐腐蚀性的同步提升，细晶粒使屈服强度、极限抗拉强度、伸长率和腐蚀速率显著提高，分别达到103MPa、223MPa、30.5%和1.5843mm/y，耐腐蚀性能的增强归因于细晶粒形成致密的腐蚀产物膜以及高密度晶界对腐蚀的阻挡效应。这表明Mg-1Zn-1Ca 合金在生物医学材料中具有广阔的应用前景。

关键词： Mg-Zn-Ca合金等通道转角挤压晶粒细化腐蚀行为力学性能

A Generalization of Haussler's Convolution Kernel——Mapping Kernel and Its Application to Tree Kernels

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Journal of Computer Science & Technology 2010年第5期25卷 1040-1054页

作者： Kilho shin Tetsuji Kuboyama Graduate School of Applied Informatics University of HyogoKobeJapan Carnegie Mellon CyLab PittsburghU.S.A Computer Center Gakushuin UniversityTokyoJapan

Haussler＇s convolution kernel provides an effective framework for engineering positive semidefinite kernels, and has a wide range of *** the other hand,the mapping kernel that we introduce in this paper is its natural generalization,and will enlarge the range of application *** main theorem with respect to positive semidefiniteness of the mapping kernel（1） implies Haussler＇s theorem as a corollary,（2） exhibits an easy-to-check necessary and sufficient condition for mapping kernels to be positive semidefinite,and（3） formalizes the mapping kernel so that significant flexibility is provided in engineering new *** an evidence of the effectiveness of our results,we present a framework to engineer tree *** tree is a data structure widely used in many applications,and tree kernels provide an effective method to analyze tree-type ***,not only is the framework important as an example but also as a practical research *** description of the framework accompanies a survey of the tree kernels in the literature,where we see that 18 out of the 19 surveyed tree kernels of different types are instances of the mapping kernel,and examples of novel interesting tree kernels.

关键词： kernel convolution kernel tree edit distance

URECA:Efficient Resource Location Middleware for Ubiquitous Environment

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Journal of Computer Science & Technology 2008年第6期23卷 929-943页

作者： Donggeon Noh Heonshik shin Mobile System and Software Laboratory School of Computer Science and EngineeringSeoul National University

We describe an effective resource location framework for ubiquitous computing environments populated by a diverse set of networks, devices, services and computational entities. Our framework provides context adaptation with the aid of a middleware service to improve the quality of resource location. A resource location protocol suitable to each type of network locates resource effectively by means of dynamic reconfiguration to the current context. Our framework is also refined by support for interoperability between different types of resource location protocols occurring across a hybrid ubiquitous network. These characteristics also reduce the control overhead for resource location, saving resource, decreasing latency and permitting a considerable degree of scalability.

关键词： resource location resource discovery middleware context-aware interoperability ubiquitous

Evaluation of leaching characteristic and kinetic study of lithium from lithium aluminum silicate glass-ceramics by naOH

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Journal of Environmental Sciences 2021年第9期33卷 98-110页

作者： Dongseok Lee Sung-Ho Joo Dong Ju shin Shun Myung shin Resources Recovery Research Center Korea Institute of Geoscience and Mineral Resources(KIGAM)124 Gwahak-roYuseong-guDaejeon 34132Republic of Korea Department of Resources Recycling University of Science and Technology(UST)217Gajeong-roYuseong-guDaejeon 34113Republic of Korea

The behavior and mechanism of Li leaching from lithium aluminum silicate glass-ceramics which can be used as a secondary source of Li using aqueous naOH solution was *** Li leaching efficiency is increased with increasing concentration of naOH, specific surface area, and reaction *** leached under optimum conditions, 2 mol/L naOH, 53 μm particle undersize, 1:10 solid/liquid ratio, 250 r/min stirring speed, 100℃ reaction temperature, 12 hr, the Li leaching efficiency was approximately 70%.However, when the leaching experiment was performed for 48 hr, the concentration of Li+ ions contained in the leach liquor decreased from 1160 to 236 mg/*** investigate the origin of this phenomenon, the obtained leach residue was analyzed by X-ray diffraction, scanning electron microscopy, and energy-dispersive X-ray *** analyses show that zeolite was formed around the lithium aluminum silicate glass-ceramics, which affected the leaching of by adsorbing Li+ *** addition, using the shrinking-core model and the Arrhenius equation, the leaching reaction with naOH was found to depends on the chemical reaction of the two reactants, with a higher than 41.84 kJ/mol of the activation energy.

关键词： Lithium aluminum silicate glass-ceramics naOH Lithium Leaching

Tunable ferroelectricity in oxygen-deficient perovskites with Grenier structure

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npj Computational Materials 2023年第1期9卷 118-125页

作者： Yongjin shin Giulia Galli Pritzker School of Molecular Engineering University of ChicagoChicagoIL 60637USA Department of Chemistry University of ChicagoChicagoIL 60637USA Center for Molecular Engineering and Materials Science Division Argonne National LaboratoryLemontIL 60439USA

Using first-principles calculations,we predict that tunable ferroelectricity can be realized in oxide perovskites with the Grenier structure and ordered oxygen ***,we show that R_(1/3)A_(2/3)FeO_(2.67) solids(where R is a rare-earth ion and A an alkaline-earth cation)exhibit polar phases,with a spontaneous polarization tunable by an appropriate choice of R and *** find that larger cations combined with small R elements lead to a maximum in the polarization and to a minimum in the energy barriers required to switch the sign of the *** arises from cooperative distortions of octahedral and tetrahedral units,where a combination of rotational and sliding modes controls the emergence of polarization within three-dimensional connected *** results indicate that polar Grenier phases of oxide perovskites are promising materials for microelectronic applications and,in general,for the study of phenomena emerging from breaking inversion symmetry in solids.

关键词： polarization ferroelectric structure

Polymer Surface Treatment by Atmospheric Pressure Low Temperature Surface Discharge Plasma:Its Characteristics and Comparison with Low Pressure Oxygen Plasma Treatment

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Plasma Science and Technology 2007年第2期9卷 181-189页

作者： AtsushiKUWABARA shin-ichiKURODA HitoshiKUBOTA Department of Chemistry Faculty of EngineeringGunma UniversityTenjin-cho 1-5-1KiryuGunma 376-8515Japan

The polymer treatment with a low-temperature plasma jet generated on the atmospheric pressure surface discharge （SD） plasma is performed. The change of the surface property over time, in comparison with low pressure oxygen （O2） plasma treatment, is examined. As one compares the treatment by atmospheric pressure plasma to that by the low pressure O2 plasma of PS （polystyrene） the treatment effects were almost in complete agreement. However, when the atmospheric pressure plasma was used for PP（polypropylene）, it produced remarkable hydrophilic effects.

关键词： atmospheric-pressure plasma low-temperature plasma plasma jet polymer surface treatment

Euler Product Expressions of Absolute Tensor Products of Dirichlet L -Functions

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Parameter estimation for building energy models using GRcGAN

Advances in Pure Mathematics（理论数学进展） 2024年第6期 451页

作者： Hidenori Tanaka shin-ya Koyama

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Building Simulation 2023年第4期16卷 629-639页

作者： Hansol shin Cheol-Soo Park Department of Architecture and Architectural Engineering College of EngineeringSeoul National UniversitySeoul08826R.O.Korea Institute of Construction and Environmental Engineering Institute of Engineering ResearchCollege of EngineeringSeoul National UniversitySeoul08826R.O.Korea

Parameter estimation methods can be classified into(1)manual(trial-and-error),(2)numerical optimization(optimization,sampling),(3)Bayesian inference(Bayes filter,Bayesian calibration),and(4)machine learning(generative model).Bayesian calibration has been widely used because it can capture stochastic nature of uncertain ***,the results of Bayesian calibration could be biased by(1)the prior distribution assumed by the expert’s subjective judgment;(2)the likelihood function that cannot always describe the true likelihood;and(3)the posterior distribution approximation method,such as the Markov Chain Monte Carlo,which requires significant computation *** overcome this,a new approach using a generator-regularized continuous conditional generative adversarial network(GRcGAN)is presented in this *** target parameters of the DOE reference building model were *** was trained to estimate uncertain parameters using simulated monthly electricity and gas *** can successfully estimate five uncertain parameters based on 1,000 training data *** proposed approach presents a potential for stochastic parameter estimation.

关键词： generative adversarial networks generative model parameter estimation inverse problem model calibration parameter uncertainty