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Progranulin regulation of autophagy contributes to its chondroprotective effect in osteoarthritis

Genes & Diseases 2023年第4期10卷 1582-1595页

作者： Yiming Pan Yuyou Yang Mengtian Fan Cheng Chen Rong Jiang Li Liang Menglin Xian biao kuang Nana Geng Naibo Feng Lin Deng Wei Zheng Fengmei Zhang Xiaoli Li Fengjin Guo Department of Cell Biology and Genetics Core Facility of Development BiologyChongqing Medical UniversityChongqing 400016China Department of Orthopaedics The 1st Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Laboratory of Stem Cells and Tissue Engineering Chongqing Medical UniversityChongqing 400016China Department of Orthopaedics The 2nd Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Chongqing Medical University Laboratory Animal Center Chongqing Medical UniversityChongqing 400016China

Progranulin(PGRN)is a multifunctional growth factor involved in many physiolog-ical processes and disease states.The apparent protective role of PGRN and the importance of chondrocyte autophagic function in the progression of osteoarthritis(OA)led us to investi-gate the role of PGRN in the regulation of chondrocyte autophagy.PGRN knockout chondro-cytes exhibited a deficient autophagic response with limited induction following rapamycin,serum starvation,and IL-1b-induced autophagy.PGRN-mediated anabolism and suppression of IL-1b-induced catabolism were largely abrogated in the presence of the BafA1 autophagy inhibitor.Mechanistically,during the process of OA,PGRN and the ATG5eATG12 conjugate form a protein complex;PGRN regulates autophagy in chondrocytes and OA through,at least partially,the interactions between PGRN and the ATG5eATG12 conjugate.Furthermore,the ATG5eATG12 conjugate is critical for cell proliferation and apoptosis.Knockdown or knockout of ATG5 reduces the expression of ATG5eATG12 conjugate and inhibits the chondroprotective effect of PGRN on anabolism and catabolism.Overexpression of PGRN partially reversed this effect.In brief,the PGRN-mediated regulation of chondrocyte autophagy plays a key role in the chondroprotective role of PGRN in OA.Such studies provide new insights into the pathogen-esis of OA and PGRN-associated autophagy in chondrocyte homeostasis.

关键词： Anabolism ATG12 ATG5 Autophagy Catabolism Osteoarthritis PGRN

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Fretting wear behaviour of machined layer of nickel-based superalloy produced by creep-feed profile grinding

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Chinese Journal of Aeronautics 2022年第10期35卷 401-411页

作者： Weijie kuang Qing MIAO Wenfeng DING Yanjun ZHAO biao ZHAO Xuebing WEN Shaopeng LI National Key Laboratory of Science and Technology on Helicopter Transmission Nanjing University of Aeronautics and AstronauticsNanjing 210016China College of Mechanical Engineering Suzhou University of Science and TechnologySuzhou 215009China State Key Laboratory of Super abrasives Zhengzhou Research Institute for Abrasive&Grinding Co.LtdZhengzhou 450001China AECC Xi'An Aero-Engine LTD Xi'an 710021China

Fretting wear has an adverse impact on the fatigue life of turbine blade roots.The current work is to comparatively investigate the fretting wear behaviour of the nickel-based superalloy surfaces produced by polishing and creep-feed profile grinding,respectively,in terms of surface/subsurface fretting damage,the friction coefficient,wear volume and wear rate.Experimental results show that the granulated tribolayer aggravates the workpiece wear,while the flat compacted tribolayer enhances the wear resistance ability of workpiece,irrespective of whether the workpiece is processed by polishing or grinding.However,the wear behaviors of tribolayers are different.For the polished surface,when the normal load exceeds 100 N,the main defects are crack,rupture,delamination and peeling of workpiece materials;the wear mechanism changes from severe oxidative wear to fatigue wear and abrasive wear when the loads increase from 50 to 180 N.As for the ground surface,the main wear mechanism is abrasive wear.Particularly,the ground surface possesses better wear-resistant ability than the polished surface because the former has the lower values in coefficient friction(0.23),wear volume(0.06×10^(6)μm^(3))and wear rate(0.25×10^(-16)Pa^(-1)).Finally,an illustration is given to characterize the evolution of wear debris on such nickel-based superalloy on the ground surface.

关键词： Creep-feed profile grinding Fretting wear Nickel-based superalloy Tribolayer Wear debris

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富氧双侧吹铜熔池熔炼炉上升烟道结渣的矿物学分析

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矿冶 2024年第3期33卷 438-447页

作者：张敏匡彪李东波胡建杭杨贵杨鹏昆明理工大学冶金节能减排教育部工程研究中心昆明650093 云南铜业股份有限公司西南铜业分公司昆明650102

富氧双侧吹铜熔池熔炼炉上升烟道烟尘结渣严重,阻塞烟道,是制约熔炼炉长周期高负荷高作业率生产的关键因素。分别利用X射线荧光光谱仪、X射线衍射仪、扫描电子显微镜和电子探针显微分析仪,研究富氧双侧吹铜熔池熔炼炉上升烟道结渣烟尘... 详细信息

富氧双侧吹铜熔池熔炼炉上升烟道烟尘结渣严重,阻塞烟道,是制约熔炼炉长周期高负荷高作业率生产的关键因素。分别利用X射线荧光光谱仪、X射线衍射仪、扫描电子显微镜和电子探针显微分析仪,研究富氧双侧吹铜熔池熔炼炉上升烟道结渣烟尘的化学成分、物相组成及微观形貌。结果表明,上升烟道烟尘的主要元素为Fe、Cu、Pb、Zn和O,主要物相是(Cu, Zn, Fe)Fe_(2)O_(4)和Fe_(3)O_(4),表面微观形貌呈致密无孔洞。结合生产过程工况条件,对烟道结渣原因进行分析,认为富氧双侧吹铜熔池熔炼炉生产过程中,富氧空气通过喷嘴鼓入炉内,强烈搅动熔体,熔体中的气泡破裂逸出,喷溅形成的小颗粒熔渣与冰铜被热气流带入上升烟道,熔渣与冰铜被氧化为高熔点的(Cu, Zn, Fe)Fe_(2)O_(4)和Fe_(3)O_(4),在烟道壁面遇冷凝结形成结渣;同时,熔炼炉负压运行,入炉原料水分低,铜精矿等未反应的粉料被烟气带入到上升烟道高温段,在氧化气氛下反应生成黏结结渣;此外,原料中Pb、Zn、As等易挥发性物质进入烟道,被氧化为低熔点化合物后与高熔点的(Cu, Zn, Fe)Fe_(2)O_(4)和Fe_(3)O_(4)黏结在一起,形成致密的结渣烟尘。因此,在冶炼生产中,建议通过控制入炉原料水分和杂质含量,减少粉料入炉;控制渣型和熔炼温度,减少熔体喷溅;使用喷嘴燃烧重油,烧熔结渣烟尘,保证生产正常稳定运行。

关键词：铜冶炼烟道结渣烟尘工艺矿物学富氧双侧吹熔池熔炼

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Nitrosoarenes as Nitrogen Source for Generation of Sulfonamides with the Insertion of Sulfur Dioxide under Metal-Free Conditions

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Chinese Journal of Chemistry 2020年第10期38卷 1098-1102页

作者： Xuefeng Wang Yanmei Lin Jin-biao Liu Fu-Sheng He Yunyan kuang Jie Wu Department of Chemistry Fudan University2005 Songhu RoadShanghai 200438China Schoo/of Pharmaceutical and Materials Engineering&Institute for Advanced Studies Taizhou University1139 Shifu AvenueTaizhouZhejiang 318000China School of Metollurgical and Chemical Engineering Jiangxi University of Science and TechnologyGanzhouJiangxi 341000China State Key Laboratory of Organometallic Chemistry Shanghai Institute of Organic ChemistryChinese Academy of Sciences345 Lingling RoadShanghai 200032China

Summary of main observation and conclusion A metal-free reaction of nitrosoarenes,aryldiazonium tetrafluoroborates,and sulfur dioxide under mild conditions is developed,giving rise to sulfonamides in moderate to good yields.This transformation proceeds eficiently at room temperature in the presence of cyclohexa-1,4-diene with a broad reaction scope.Good functional group compatibility is observed,including cyano,halo,and ester.A plausi-ble mechanism involving a radical process with the insertion of sulfur dioxide is proposed,and cyclohexa-1,4-diene serves as the reductant during the transformation.

关键词： dioxide sulfur Sulfur

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THE GPL-STABILITY OF RUNGE-KUTTA METHODS FORDELAY DIFFERENTIAL SYSTEMS

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Journal of Computational Mathematics 2000年第1期18卷 75-82页

作者： biao Yang Lin Qiu Jiao-xun kuang(Department of Mathematics, Shanghai Normal University, Shanghai 200234, China.) 上海师范大学上海 200234

Presents a study which dealt with stability of the implicit Runge-Kutta methods for the numerical solutions of the systems of delay differential equations. Stability behavior of the methods; Exponential solutions of t... 详细信息

关键词： delay differential equation implicit Runge-Kutta methods GPL-stability

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结构变迁视角下的粤港澳跨域协作治理

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地理科学 2023年第7期43卷 1174-1184页

作者：匡贞胜赵彪孙斌栋郑州大学政治与公共管理学院/公共管理研究中心河南郑州450001 中国社会科学院中国边疆研究所北京100101 华东师范大学中国行政区划研究中心上海200241

港澳回归以来粤港澳政府间合作面临诸多困难,因此中国中央政府以纵向嵌入的方式助推大湾区建设来破解跨域协作难题。基于多维动态过程视角,系统分析了三地产业结构、府际关系、社会文化变迁与跨域协作的内在关联,认为产业结构消长压缩... 详细信息

港澳回归以来粤港澳政府间合作面临诸多困难,因此中国中央政府以纵向嵌入的方式助推大湾区建设来破解跨域协作难题。基于多维动态过程视角,系统分析了三地产业结构、府际关系、社会文化变迁与跨域协作的内在关联,认为产业结构消长压缩了经济合作空间,社会文化交融不足弱化了三地协作动力,而全面管治权与中国香港国安法的实施则重构了府际间政治距离,这为中央政府高位推动跨域协作提供了必要性与可行性。研究指出,自上而下的国家战略虽可在短期内调动各方积极性,但受制于地方政府理性人与代理人的角色关系,难以维持跨域协作的长期能动性。未来应树立动态过程管控理念,建立制度性跨域协作框架与功能性跨域治理机构,督促港澳采取积极产业政策,并高度重视三地社会与文化交融问题。

关键词：粤港澳大湾区结构变迁跨域协作府际关系

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Inhibitory effects of baicalein against herpes simplex virus type 1

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Acta Pharmaceutica Sinica B 2020年第12期10卷 2323-2338页

作者： Zhuo Luo Xiu-Ping kuang Qing-Qing Zhou Chang-Yu Yan Wen Li Hai-biao Gong Hiroshi Kurihara Wei-Xi Li Yi-Fang Li Rong-Rong He Guangdong Engineering Research Center of Chinese Medicine&Disease Susceptibility College of PharmacyCollege of PharmacyJinan UniversityGuangzhou 612505China International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Chinese Ministry of Education(MOE) College of PharmacyJinan UniversityGuangzhou 612505China Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research College of PharmacyJinan UniversityGuangzhou 612505China Yunnan University of Traditional Chinese Medicine Kunming 650550China

Herpes simplex virus type 1(HSV-1)is a ubiquitous and widespread human pathogen,which gives rise to a range of diseases,including cold sores,corneal blindness,and encephalitis.Currently,the use of nucleoside analogs,such as acyclovir and penciclovir,in treating HSV-1 infection often presents limitation due to their side effects and low efficacy for drug-resistance strains.Therefore,new anti-herpetic drugs and strategies should be urgently developed.Here,we reported that baicalein,a naturally derived compound widely used in Asian countries,strongly inhibited HSV-1 replication in several models.Baicalein was effective against the replication of both HSV-1/F and HSV-1/Blue(an acyclovir-resistant strain)in vitro.In the ocular inoculation mice model,baicalein markedly reduced in vivo HSV-1/F replication,receded inflammatory storm and attenuated histological changes in the cornea.Consistently,baicalein was found to reduce the mortality of mice,viral loads both in nose and trigeminal ganglia in HSV-1 intranasal infection model.Moreover,an ex vivo HSV-1-EGFP infection model established in isolated murine epidermal sheets confirmed that baicalein suppressed HSV-1 replication.Further investigations unraveled that dual mechanisms,inactivating viral particles and inhibiting IκB kinase beta(IKK-β)phosphorylation,were involved in the anti-HSV-1 effect of baicalein.Collectively,our findings identified baicalein as a promising therapy candidate against the infection of HSV-1,especially acyclovir-resistant strain.

关键词： Anti-HSV-1 Baicalein Viral inactivation IKK-βphosphorylation NF-kβactivation HSV-1 infection

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Incidence and mortality of liver cancer in China, 2010

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Chinese Journal of Cancer 2014年第8期33卷 388-394页

作者： kuang-Rong Wei Xia Yu Rong-Shou Zheng Xia-biao Peng Si-Wei Zhang Ming-Fang Ji Zhi-Heng Liang Zhi-Xiong Ou Wan-Qing Chen Cancer Institute Zhongshan Municipal People’s HospitalZhongshan Guangdong National Central Cancer Registry National Cancer CenterBeijing Digestive Internal Department Zhongshan Municipal People’s HospitalZhongshan Guangdong

Liver cancer is a common malignant tumor in China and a major health concern. We aimed to estimate the liver cancer incidence and mortality in China in 2010 using liver cancer data from some Chinese cancer registries and provide reference for liver cancer prevention and treatment. We collected and evaluated the incidence and mortality data of liver cancer in 2010 from 145 cancer registries, which were included in the 2013 Chinese Cancer Registry Annual Report, calculated crude, standardized, and truncated incidences and mortalities, and estimated new liver cancer cases and deaths from liver cancer throughout China and in different regions in 2010 from Chinese practical population. The estimates of new liver cancer cases and deaths were 358,840 and 312,432, respectively, in China in 2010. The crude incidence, age-standardized rate by Chinese standard population(ASR China), and age-standardized rate by world standard population(ASR world) were 27.29/100,000, 21.35/100,000, and 20.87/100,000, respectively; the crude, ASR China, and ASR world mortalities were 23.76/100,000, 18.43/100,000, and 18.04/100,000, respectively. The incidence and mortality were the highest in western regions, higher in rural areas than in urban areas, and higher in males than in females. The age-specific incidence and mortality of liver cancer showed a rapid increase from age 30 and peaked at age 80-84 or 85+. Our results indicated that the 2010 incidence and mortality of liver cancer in China, especially in undeveloped rural areas and western regions, were among high levels worldwide. The strategy for liver cancer prevention and treatment should be strengthened.

关键词：中国标准死亡率肝癌世界人口死亡病例恶性肿瘤西部地区农村地区

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Transforming growth factor-β1 involved in urotensin Ⅱ-induced phenotypic differentiation of adventitial fibroblasts from rat aorta

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Chinese Medical Journal 2010年第24期123卷 3634-3639页

作者： ZHANG Yong-gang HU Yan-chao MAO Yan-yan WEI Rui-hong BAO Shi-lin WU Li-biao kuang Ze-jian Department of Cardiovascular Diseases First Affiliated Hospital Shantou University Medical College Shantou Guangdong 515041 China Laboratory of Molecular Biology and Cardiology First Affiliated Hospital Shantou University Medical College Shantou Guangdong 515041 China Department of Internal Medicine Second Affiliated Hospital Shantou University Medical College Shantou Guangdong 515041 China

Background Urotensin Ⅱ （UⅡ） is a new vasoconstrictive peptide that may activate the adventitial fibroblasts.Transforming growth factor-β1 （TGF-β1） is an important factor that could induce the phenotypical transdifferentiation of adventitial fibroblasts. This study aimed to explore whether TGF-β1 is involved in UⅡ-induced phenotypic differentiation of adventitial fibroblasts from rat aorta.Methods Adventitial fibroblasts were prepared by the explant culture method. TGF-β1 protein secretion from the cells was determined by enzyme-linked immunosorbent assay （ELISA）. The mRNA and protein expression of α-smooth nuscle actin （α-SM-actin）, the marker of phenotypic differentiation from fibroblasts to myofibroblasts, were determined using real-time quantitative RT-PCR （real-time RT-PCR） and Western blotting, respectively.Results UⅡ stimulated the secretion of TGF-β1 in cultured adventitial fibroblasts in a time-dependent manner. The secretion reached a peak at 24 hours, was higher by 69.8% （P ＜0.01）, than the control group. This effect was also concentration dependent. Maximal stimulation was reached at 10-8 mol/L of UⅡ （P ＜0.01）, which was increased by 59.9%,compared with in the control group （P ＜0.01）. The secretion of TGF-β1 induced by UⅡ was significantly blocked by SB-710411 （10-7 mol/L）, a specific antagonist of UⅡ receptor. In addition, both UⅡ （10-8 mol/L） and TGF-β1 significantly stimulated α-SM-actin mRNA and protein expression. Moreover, the α-SM-actin induced by UⅡ was inhibited by the specific neutralizing antibody （20 μg/ml） of TGF-β1, while the α-SM-actin expression stimulated by TGF-β1 （20 ng/ml）was inhibited by SB-710411 （10-7 mol/L）, the UⅡ receptor antagonist.Conclusion This study suggests that UⅡ could induce TGF-β1 secretion in adventitial fibroblasts via UT activation, and TGF-β1 might be involved in phenotypic differentiation from adventitial fibroblasts into myofibroblasts induced by UⅡ, and TGF-β1 signaling might be one of the important pathways by which UⅡ is involved in vascular fibrosis.

关键词： urotensin Ⅱ transforming growth factor-β1 adventitial fibroblasts phenotypic differentiation vascular fibrosis

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县级行政区划规模偏小对京津冀协同发展的影响

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地理与地理信息科学 2023年第1期39卷 97-104页

作者：赵彪王开泳匡贞胜中国社会科学院中国边疆研究所北京100101 中国科学院地理科学与资源研究所区域可持续发展分析与模拟重点实验室北京100101 郑州大学政治与公共管理学院河南郑州450001

县级政区是国家治理的空间基础,该文基于行政区划视角,通过县级行政区划的属性分析,发现县级行政区划规模偏小是制约当前京津冀协同发展的突出问题,县级政区过多、过小阻碍中小城市的正常发育,中小城市发展迟缓加剧了城镇体系的不协调... 详细信息

县级政区是国家治理的空间基础,该文基于行政区划视角,通过县级行政区划的属性分析,发现县级行政区划规模偏小是制约当前京津冀协同发展的突出问题,县级政区过多、过小阻碍中小城市的正常发育,中小城市发展迟缓加剧了城镇体系的不协调和区域发展不均衡问题。主要原因有:辖区面积偏小造成县(市)资源配置能力较弱,管理幅度较大增加了组织管理的复杂程度,行政边界较多抬高了生产要素的流通成本。据此提出政策建议:合理确定县级政区规模,推进小县合并,缓解县(市)内生发展动力不足问题;适时推进建制转换,积极培育中心城市,缓解中等城市发展能级不足问题;有序开展体制改革,理顺行政管理体制,缓解公共服务供给能力不足问题。

关键词：行政区划县级政区京津冀协同发展

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