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A WW Domain-c.ntaining Protein Forms Immune Nuc.ear Bodies against Begomoviruses

Molec.lar Plant 2018年第12期11卷 1449-1465页

作者： lara P. c.lil lana P.S. Quadros Thais c. Araujo c.ristiane E.M. Duarte bianc. c. gouveia-mageste Jose c.eydson F. Silva Otavio J.B. Brustolini Ruan M. Teixeira c.ue N. Oliveira Rafael W.M.M. Milagres Gilberto S. Martins Joanne c.ory Pedro A.B. Reis Joao Paulo B. Mac.ado Elizabeth P.B. Fontes Departament of Bioc.emistry and Molec.lar Biology Universidade Federal de Vigosa Vigosa Minas Gerais 36570-000 Brazil Nati onal In stitute of Sc.enc. and Tec. no logy in Plant-Pest Interac.ions Bioagro Universidade Federal de Vicosa Vinosa Minas Gerais 36570-000 Brazil Departament of Genetic. Universidade Federal do Rio de Janeiro Rio de Janeiro Rio de Janeiro Brazil Howard Hughes Medic.l Institute and Plant Biology Laboratory The Salk Institute of Biological Studies La Jolla CA 92037 USA Agronomy Institute Universidade Federal de Vigosa Campus Florestal Florestal Minas Gerais 35690-000 Brazil These authors c.ntributed equally to this artic.e

The bipartite begomoviruses (Geminiviridae family), whic. are DNA viruses that replic.te in the nuc.eus of infec.ed c.lls, enc.de the nuc.ear shuttle protein (NSP) to fac.litate the transloc.tion of viral DNA from the nuc.eus to the c.toplasm via nuc.ear pores. This intrac.llular traffic.ing of NSP-DNA c.mplexes is ac.essorized by the NSP-interac.ing guanosine triphosphatase (NIG) at the c.tosolic.side. Here, we report the nuc.ear redistribution of NIG by AtWWPI, a WW domain-c.ntaining protein that forms immune nuc.ear bodies (NBs) against begomoviruses. We demonstrated that AtWWPI reloc.tes NIG from the c.toplasm to the nuc.eus where it is c.nfined to AtWWP1-NBs, suggesting that the NIG-AtWWP1 interac.ion may interfere with the NIG pro-viral func.ion assoc.ated with its c.tosolic.loc.lization. c.nsistent with this assumpti on joss AtWWPI func.i on c.ased plants more susc.ptible to begomovirus infec.ion, whereas overexpression of AWWP1 enhaneed plant resistanc. to begomovirus. Furthermore, we found that a mutant versi on of AtWWPI defec.ive for NB formatio n was noIonger c.pable of interac.i ng with and reloc.ting NIG to the nuc.eus and lost its immune func.ion against begomovirus. The antiviral func.ion of AtWWP1-NBs, however, c.uld be antagonized by viral infec.ion that induc.d either the disruption or a dec.ease in the number of AtWWP1-NBs. c.llec.ively, these results led us to propose that AtWWPI organizes nuc.ear struc.ures into nuc.ear foc., whic. provide intrinsic.immunity against begomovirus infec.ion.

关键词： nuc.ear bodies antiviral immunity begomovirus NIG AtWWP1

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