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QTLs for Sugar Content of Stalk in Sweet Sorghum(Sorghum bicolor ***)

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Agricultural Sciences in China 2006年第10期5卷 736-744页

作者： bian yun-long Yazaki Seiji Inoue Maiko CAI Hong-wei Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology Department of Agronomy Yangzhou University Yangzhou 225009 P.R. China Forage Crop Research Institute Japan Grassland Agriculture and Forage Seed Association 388-5 Higashiakata Nasushiobara Tochigi 329-2742 Japan

High sugar content of sorghum stalk is an important factor in the sorghum silage production. To identify the genomic regions controlling sugar content and to develop molecular markers linked to sugar content in sweet sorghum, we used an F2：3 segregating population consisting of 207 individuals derived from a cross between a high sugar content inbred line, Early Folger, and a normal inbred line, N32B, for genetic linkage mapping and quantitative trait locus （QTL） analysis. We constructed a genetic linkage map spanning 983.5 cM based on a total of 327 markers comprising 31 restriction fragment length polymorphism （RFLP） markers, 254 amplified fragment length polymorphism （AFLP） markers, and 42 simple sequence repeat （SSR） markers. In the 20 linkage groups detected, 98.2% of markers aligned to the 10 linkage groups of sorghum. Variations in sugar content at different growth stages and among internodes suggested that the sugar content of middle internodes is stable and suitable for measuring at early dough stage. The broad sense heritability （hB0 of sugar content was 0.64 and 0.62 estimated from the data of F3 families and each parent in 2003 and 2004. We identified one and two QTLs accounting for 22.2 to 25.0% of phenotypic variance using simple interval mapping method in 2003 and 2004, respectively. These two QTLs showed a negative additive effect, and over-dominance effect. A QTL on LG-D was detected in both two years. Above results will be help us to understand the genetic mechanism of sugar content in sorghum and the QTL detected in this study might be useful in the improvement of sugar content by marker-assisted selection.

关键词： sugar content molecular marker QTL sorghum

Application and evaluation of improved surgical aseptic technique curriculum in specialty nurse training in Henan Province

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International Journal of Nursing Sciences 2016年第3期3卷 281-284页

作者： Bing Bai Sheng-yun Li bian-yun Wang Zeng-Mei Zhang Li-Qun Sun The First Affiliated Hospital of Zhengzhou University ZhengzhouHenan Province450052China

Objective:To develop and apply a novel surgical aseptic technique curriculum in specialty training for operating room(OR)***:As surgical environments were improved,related specifications were *** proposed surgical aseptic procedure was discussed,improved,and recorded as procedure *** the new training program,traditional theoretical lecture training was replaced with sequential theoretical lessons and video demonstration,operation presentation,and skill *** the end of the training,the trainees were given questionnaires to assess training *** in student satisfaction regarding the proposed training program and conventional training programs were then ***:A GPA of 3.61±0.58 was recorded after the new training program was *** minimum and maximum average scores of each item were 3.05±0.43 and 4.61±0.33,respectively The satisfaction rate of the trainees in 2013 was significantly higher than that in 2011(t=2.14,P=0.039).Conclusion:Novel surgical aseptic technique and application in the curriculum design of training for OR nurses should be developed to enhance their mastery of theoretical and practical skills and to modify their behaviors.

关键词： Specialized nurse Aseptic technique Operating room Training method

Genetic Analysis of the Resistance to Aspergillus flavus Infection in Maize (Zea mays L.)

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Agricultural Sciences in China 2009年第6期8卷 761-765页

作者： DENG De-xiang JIANG Si-xia WANG Yi-jun bian yun-long CHEN Jian-jian JIA Bo Jiangsu Province Key Laboratory of Crop Genetics and Physiology~Key Laboratory of Plant Functional Genomics Ministry of Education Yangzhou University Yangzhou 225009 P.R. China Yangzhou Environment and Resources Technical College Yangzhou 225009 P.R. China

Maize （Zea mays L.）, one of main crops in the world, is easily susceptible to Aspergillusflavus （Link： fr） infection, resulting huge loses worldwide. Breeding for A. flavus resistance has been proved an efficient way to solve the problem of aflatoxin contamination. Genetic analysis of the sources of resistance to *** in maize is necessary for this purpose. The complete diallel crosses of 6 inbred lines with different resistance to *** infection were implemented. Inoculation categorical data of each cross were analyzed with the additive-dominant and additive-dominant-epitasis genetic models. Results indicated some crosses fitted the 2 major genes with additive-dominant-epitasis genetic model. Others fitted the major gene and polygene mixed model. Moreover, the additive, dominant, and epitasis effects varied in crosses. The *** resistance was controlled by both major gene and polygene.

关键词： maize Aspergillus flavus resistance genetic model

ON THE ORIGIN OF TURBULENT SECONDARY FLOW IN A STRAIGHT DUCT OF NON-CIRCULAR CROSS-SECTION

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Journal of Hydrodynamics 1993年第3期5卷 98-111页

作者： Zhao, Lie Wang, Famin bian, yungui Yu, Xin Inst of Mechanics Academia Sinica Beijing China

The origin of turbulent secondary flow in a straight duct of non-circular cross-section was discussed from a theoretical standpoint and examined from a numerical simulation of the turbulent flow in a square duct. It was reached that the driving force of such secondary flow comes from the second-order terms of the difference in gradients of normal and transverse Reynolds stresses in the axial vorticity equation. The present results obtained compare well with available experimental results for both primary and secondary flow quantities.

关键词： Ducts Pipe flow Simulation

Quantification of pancreatic exocrine function of chronic pancreatitis with secretin-enhanced MRCP

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World Journal of Gastroenterology 2013年第41期19卷 7177-7182页

作者： yun bian Li Wang Chao Chen Jian-Ping Lu Jia-Bao Fan Shi-Yue Chen Bing-Hui Zhao Department of Radiology Changhai Hospitalthe Second Military Medical University Department of laboratory the 411st Hospital of Chinese PLA

AIM:To obtain reference values for pancreatic flow output rate(PFR)and peak time(PT)in healthy volunteers and chronic pancreatitis(CP);to correlate quantification of secretin enhanced magnetic resonance cholangiopancreatography(SMRCP)of pancreatic fluid output following secretin with fecal elastase-1(FE-1)***:The present study includes 53 subjects comprised of 17 healthy individuals and 36 patients with CP from April 2011 to January *** 36 patients with CP were divided into three groups of mild CP(n=14),moderate CP(n=19)and advanced CP(n=3)by M-ANNHEIM classification for CP..Fifty-three cases underwent FE-1 test and magnetic resonance imaging using 3.0 T-device(Signa EXCITE,GE Healthcare).Coronal T2-weighted single-shot turbo spin-echo,spiratory triggered,covering the papillae,duodenum and small *** was performed with a heavily T2-weighted fat-suppressed long TE HASTE sequence (thick slab 2D MRCP sequence),repeated every 2 min up to 11 min after 0.1 mL/kg secretin injection(Secrelux,Sanochemia,Germany).FE-1 test used sandwich enzyme-linked immunosorbent assay(ELISA)test(***,Germany).RESULTS:A good linear correlation showed between the calculated volume and the actual volume by Phantom ***-three paired Quantification of secretin enhanced magnetic resonance cholangiopancreatography(MRCPQ)and FE-1 data sets were *** mean FE-1 of 53 cases was 525.41±94.44μg/g for 17 healthy volunteers,464.95±136.13μg/g for mild CP,301.55±181.55μg/g for moderate CP,229.30±146.60μg/g for advanced ***,there was statistically significant difference in FE-1(P=0.0001)between health and *** mean values of PFR and PT were 8.18±1.11 mL/min,5.76±1.71 min for normal;7.27±2.04 mL/min,7.71±2.55 min for mild CP;4.98±2.57 mL/min,9.10±3.00 min for moderate CP;4.13±1.83 mL/min,12.33±1.55 min for advanced ***,statistically significant difference in PFR(P=0.0001)and PT(P=0.0001)was observed between health and ***,there was correlati

关键词： Secretin Magnetic resonance cholangiopancreatography Pancreatic exocrine function Chronic pancreatitis Magnetic resonance imaging

蒙古黄芪中黄酮类成分抗超氧阴离子活性研究及构效关系分析

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中国药学杂志 2008年第4期43卷 256-259页

作者：卞云云李萍中国药科大学现代中药教育部重点实验室、生药学教研室南京210038

目的研究蒙古黄芪[Astragalus membranaceus(Fish·)Bge·var·mongholicus(Bge·)Hsiao]中有效成分对超氧阴离子(O2-)的清除能力,并探讨黄酮类物质抗氧化能力与结构之间的关系。方法对蒙古黄芪中的黄酮类成分进行分离,通过化学发光法... 详细信息

目的研究蒙古黄芪[Astragalus membranaceus(Fish·)Bge·var·mongholicus(Bge·)Hsiao]中有效成分对超氧阴离子(O2-)的清除能力,并探讨黄酮类物质抗氧化能力与结构之间的关系。方法对蒙古黄芪中的黄酮类成分进行分离,通过化学发光法测定这些成分对邻苯三酚-鲁米诺-碳酸缓冲液(pH10·2)体系产生的超氧阴离子的清除能力。结果从蒙古黄芪中分离出12个黄酮类单体,分别为:槲皮素(Ⅰ),异槲皮苷(Ⅱ),芦丁(Ⅲ),山柰酚(Ⅳ)和异鼠李素(Ⅴ),芒柄花素(Ⅵ),芒柄花苷(Ⅶ),毛蕊异黄酮(Ⅷ),(3R)-8,2′-二羟基-7,4′-二甲氧基异黄烷(Ⅸ),2′,4′-二甲氧基-3′-羟基异黄烷-6-O-β-葡萄糖苷(Ⅹ),(6aR,11aR)-10-羟基-3,9-二甲氧基紫檀烷(Ⅺ),(6aR,11aR)9,10-二甲氧基紫檀烷-3-O-β-D-葡萄糖苷(Ⅻ)。结果表明,所有成分在一定浓度范围内(此浓度范围所对应的清除率在10%～90%之间),量效呈对数关系(y=alogx+b)。构效关系结果显示,化合物的分子结构决定其抗氧化活性。结论黄芪中的黄酮类成分具有明显的清除超氧阴离子活性,是黄芪抗氧化作用的主要活性成分,且黄酮类成分的清除超氧阴离子活性与其结构有明显的相关性。

关键词：蒙古黄芪黄酮抗氧化超氧阴离子

Adiponectin Attenuates Hypoxia/Reoxygenation-Induced Cardiomyocyte Injury Through Inhibition of Endoplasmic Reticulum Stress

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中国动脉硬化杂志 2011年第3期19卷 274-275页

作者： bian yun-Fei YANG Hui-Yu HAO Xiao-Yan GAO Feng ZHANG Na-Na XIAO Chuan-Shi Department of Cardiology the Second Hospital of Shanxi Medical University Taiyuan Shanxi 030001 China

Background and Aim Adiponectin(APN) is a potent cardioprotective *** present study aims to investigate the under-lying mechanism(s) for its cardioprotective *** Primary cardiomyocytes were isolated from neonatal rats and an invitro model of hypoxia-reoxygenation(H/R) was *** cardiomyocytes were randomly divided into six groups: salinegroup(control),dithiothreitol(DTT) group(5 mmol/L DTTfor 2 h),H/R group,H/R +APN group(incubation with 30 mg/LAPN,followed by H/R),H/R +APN +SB203580(SB) group(treatment with 30 mg/L APN and 5μmol/L SB,followed by H/R),and H/R +SB group(exposure to 5μmol/L SB and then H/R).Cell death was detected by measuring lactate dehydrogenase(LDH) *** expression levels of hypoxia-inducible factor-1alpha(HIF-1α) and endoplasmic reticulum(ER) stress-relatedgenes including GRP78,caspase-12,C/EBP homologus protein(CHOP),and p38 mitogen-activated protein kinase(MAPK) *** Cardiomyocytes exposed to H/R showed a significant increase in LDH leakage and HIF-1αprotein levelscompared with the control cells(P<0.05).The H/R-provoked cell death was profoundly attenuated by the pretreatment with APNalone,SB alone,or both,which was coupled with decreased expression of GRP78,caspase-12,CHOP,and p38 ***-sions These results provide new insights into the mechanism of APN-mediated cardioprotection,which may be partially due to inhibi-tion of ER stress response.

关键词：心脏治疗方法临床分析应激反应

《WHO儿童基本药物标准清单(2015年版)》与“《国家基本药物目录(2012年版)》中儿童可用药物清单”的比较和分析

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国际药学研究杂志 2016年第4期43卷 602-607页

作者：陈永法卞云云中国药科大学国际医药商学院南京211198

本文以《WHO儿童基本药物标准清单(2015年版)》和"《国家基本药物目录(2012年版)》中儿童可用药物清单"为研究对象,定量分析两目录药物品种、剂型、规格和用药信息,得知中国目录中儿童可用药物数量与WHO基本持平,药物规格更符合儿童需求... 详细信息

本文以《WHO儿童基本药物标准清单(2015年版)》和"《国家基本药物目录(2012年版)》中儿童可用药物清单"为研究对象,定量分析两目录药物品种、剂型、规格和用药信息,得知中国目录中儿童可用药物数量与WHO基本持平,药物规格更符合儿童需求,但儿童适宜剂型较少,儿童用药体质量限制、可联合用药和可替代药物信息标识较少。建议加强儿童药物剂型的研发,从根本上解决儿童适宜药物的短缺问题;完善药物临床试验,为儿童用药提供循证医学证据支持;适应儿童基本医疗卫生服务需求,制订中国儿童基本药物目录。

关键词： WHO儿童基本药物标准清单国家基本药物目录儿童用药比较研究

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磁共振对慢性胰腺炎诊断的临床研究进展

第二军医大学学报 2014年第9期35卷 1001-1006页

作者：边云王莉第二军医大学长海医院影像科上海200433 解放军411医院影像科上海200081

慢性胰腺炎(chronic pancreatitis,CP)在我国的发病率呈逐年增高的趋势,其诊断方法除结合病史、临床表现外,主要通过影像学检查。由于磁共振图像(magnetic resonance imaging,MRI)含有独特的化学结构信息,被认为比超声、计算机断层扫描(... 详细信息

慢性胰腺炎(chronic pancreatitis,CP)在我国的发病率呈逐年增高的趋势,其诊断方法除结合病史、临床表现外,主要通过影像学检查。由于磁共振图像(magnetic resonance imaging,MRI)含有独特的化学结构信息,被认为比超声、计算机断层扫描(computed tomography,CT)具有更大的潜在优越性,是近年来一种有价值的备受注目的新技术。MRI精髓在于既能显示形态学改变,亦能反映组织器官的功能性变化,甚至可提供生化过程的信息和动态的定量资料。本文就目前临床上运用磁共振(magnetic resonance,MR)诊断CP的研究现况作一综述。

关键词：慢性胰腺炎诊断磁共振成像磁共振波谱学