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Magnetic Bead‑Based Sandwich Immunoassay for Viral Pathogen Detection by Employing Gold Nanoparticle as Carrier

Journal of Analysis and Testing 2017年第4期1卷 298-305页

作者： Lei Zhan wen bi wu Chun Mei Li Cheng Zhi Huang Key Laboratory on Luminescence and Real‑Time Analytical Chemistry(Ministry of Education) College of Pharmaceutical ScienceSouthwest UniversityChongqing 400716China Chongqing Key Laboratory of biomedical Analysis(Chongqing Science&Technology Commission) College of Chemistry and Chemical EngineeringSouthwest UniversityChongqing 400715China

Enzyme-linked immunosorbent assay(ELISA)provides a convenient way for the detection of viral ***,conventional ELISA performed on mirowell plates suffers from poor sensitivity,laborious coating and complicated blocking ***,we designed a sensitive colorimetric immunoassay by taking advantages of the enrichment and isolation ability of magnetic beads(MBs)and the high loading capacity of gold nanoparticles(AuNPs)for detecting respiratory syncytial virus(RSV)as a pathogen *** was selectively captured and preconcentrated from samples with antibodies functionalized MBs,followed by binding with antibodies labeled AuNPs,which carrying a large amount of alkaline phosphatase(ALP)molecules for colorimetric signal amplification by catalyzing the dephosphorylation of non-colored pNPP to generate colored product *** optimizing the experimental conditions based on the principle of low nonspecific signal,low cost,and high sensitivity,the analytical sensitivity of the developed immunoassay can be improved to 0.27 pg/mL,which is over sevenfold higher than that of commercially available RSV ELISA kits(2 pg/mL).In addition,the total assay time was less than 2.5 h without any pretreatment,which is much more rapid than other reported ***,the proposed immunoassay holds great promise for the fabrication of rapid,sensitive,and economic method for the viral pathogen detection.

关键词： Magnetic bead Gold nanoparticles Enzyme immunoassay Signal amplification Respiratory syncytial virus

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Characteristics of the water-soluble components of aerosol particles in Hefei,China

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Journal of Environmental Sciences 2016年第4期28卷 32-40页

作者： Xue-liang Deng Chun-e Shi bi-wen wu Yuan-jian Yang Qi Jin Hong-lei Wang Song Zhu Caixia Yu Anhui Institute of Meteorology Key Laboratory of Atmospheric Science and Satellite Remote Sensing Hefei 230031 China Anhui Weather Modification Hefei 230031 China Key Laboratory for Aerosol-Cloud-Precipitation of China Meteorological Administration Nanjing University of Information Science and Technology Nanjing 210044 China Hefei Meteorological Bureau Hefei 230041 China

Size-classified daily aerosol mass concentrations and concentrations of water-soluble inorganic ions were measured in Hefei,China,in four representative months between September 2012 and August *** annual average mass concentration of 169.09μg/m^3 for total suspended particulate（TSP）was measured using an Andersen Mark-II cascade *** seasonal average mass concentration was highest in winter（234.73μg/m^3）and lowest in summer（91.71μg/m^3）.Water-soluble ions accounted for 59.49%,32.90%,48.62%and 37.08%of the aerosol mass concentration in winter,spring,summer,and fall,respectively,which indicated that ionic species were the primary constituents of the atmospheric *** four most abundant ions were NO3^-,SO4^2-,Ca^2＋ and NH4^＋.With the exception of Ca^2＋,the mass concentrations of water-soluble ions were in an intermediate range compared with the levels for other Chinese ***,nitrate,and ammonium were the dominant fine-particle species,which were bimodally distributed in spring,summer and fall;however,the size distribution became unimodal in winter,with a peak at 1.1–2.1μ*** Ca^2＋ peak occurred at approximately 4.7–5.8μm in all *** cation to anion ratio was close to 1.4,which suggested that the aerosol particles were alkalescent in *** average NO3^-/SO4^2-mass ratio was 1.10 in Hefei,which indicated that mobile source emissions were *** positive correlation coefficients between the concentrations of NH4^＋ and SO4^2-,NH4^＋ and NO3^-,SO4^2-and NO3^-,and Mg^2＋ and Ca^2＋ were also indicated,suggesting that aerosol particles may be present as（NH4）2SO4,NH4HSO4,and NH4NO3.

关键词： Aerosol Water-soluble ions Hefei Size distribution

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MUSIC算法在相干探海雷达中的应用

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武汉大学学报（理学版） 2001年第5期47卷 649-652页

作者：赵汉青文必洋吴敏武汉大学电子信息学院湖北武汉430072

根据海杂波的频谱特性 ,运用对称阵列方法中提到的思想对传统的 MU ltiple SIgnal Classification(MU SIC)算法进行了两个方面的改进 .一是对要进行特征值分解的矩阵进行重构 ,以解决有相干信号存在的问题 ;二是把算法推广到频域中执行 ... 详细信息

根据海杂波的频谱特性 ,运用对称阵列方法中提到的思想对传统的 MU ltiple SIgnal Classification(MU SIC)算法进行了两个方面的改进 .一是对要进行特征值分解的矩阵进行重构 ,以解决有相干信号存在的问题 ;二是把算法推广到频域中执行 ,以满足目标信号的背景噪声是海杂波的特性 .

关键词：多重信号分类算法相干信号海杂波相干雷达海洋回波高分辨率空间谱分析 MUSIC算法

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基于谈话“参与框架”的法庭口译员角色研究

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集美大学学报（哲学社会科学版） 2021年第4期24卷 122-128,136页

作者：温碧武集美大学外国语学院福建厦门361021

随着越来越多的外籍人士来华,我国的涉外案件随之增加,加上近年一些西方国家给中国处处设置障碍,越来越多的中国企业和个人卷入涉外诉讼中。因此,培养更加专业的法庭口译人才具有现实战略意义,而角色定位是培养专业法庭口译人才的前提... 详细信息

随着越来越多的外籍人士来华,我国的涉外案件随之增加,加上近年一些西方国家给中国处处设置障碍,越来越多的中国企业和个人卷入涉外诉讼中。因此,培养更加专业的法庭口译人才具有现实战略意义,而角色定位是培养专业法庭口译人才的前提之一。基于戈夫曼谈话“参与框架”理论,借助孙杨抗检一案公开听证会上口译员表现,从听和说两个角度,可以更好地解析法庭口译员在法庭机构话语语境中角色的构建与转换。

关键词：参与框架法庭口译员角色研究

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Effects of aggregate size distribution and carbon nanotubes on the mechanical properties of cemented gangue backfill samples under true triaxial compression

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 311-324页

作者： Qian Yin Fan wen Zhigang Tao Hai Pu Tianci Deng Yaoyao Meng Qingbin Meng Hongwen Jing Bo Meng Jiangyu wu State Key Laboratory of Intelligent Construction and Healthy Operation and Maintenance of Deep Underground Engineering China University of Mining and TechnologyXuzhou 221116China State Key Laboratory of Mining Response and Disaster Prevention and Control in Deep Coal Mines Anhui University of Science and TechnologyHuainan 232001China State Key Laboratory for Geomechanics&Deep Underground Engineering China University of Mining and Technology(Beijing)Beijing 100083China Key Laboratory of Xinjiang Coal Resources Green Mining Ministry of EducationXinjiang Institute of EngineeringUrumqi 830023China

The mechanical behavior of cemented gangue backfill materials(CGBMs)is closely related to particle size distribution(PSD)of aggregates and properties of cementitious ***,the true triaxial compression tests,CT scanning,SEM,and EDS tests were conducted on cemented gangue backfill samples(CGBSs)with various carbon nanotube concentrations(P_(CNT))that satisfied fractal theory for the PSD of *** mechanical properties,energy dissipations,and failure mechanisms of the CGBSs under true triaxial compression were systematically *** results indicate that appropriate carbon nanotubes(CNTs)effectively enhance the mechanical properties and energy dissipations of CGBSs through micropore filling and microcrack bridging,and the optimal effect appears at P_(CNT)of 0.08wt%.Taking PSD fractal dimension(D)of 2.500 as an example,compared to that of CGBS without CNT,the peak strength(σ_(p)),axial peak strain(ε_(1,p)),elastic strain energy(Ue),and dissipated energy(U_(d))increased by 12.76%,29.60%,19.05%,and90.39%,***,excessive CNTs can reduce the mechanical properties of CGBSs due to CNT agglomeration,manifesting a decrease inρ_(p),ε_(1,p),and the volumetric strain increment(Δε_(v))when P_(CNT)increases from 0.08wt%to 0.12wt%.Moreover,the addition of CNTs improved the integrity of CGBS after macroscopic failure,and crack extension in CGBSs appeared in two modes:detour and pass through the ***σ_(p)and U_(d)firstly increase and then decrease with increasing D,and porosity shows the opposite ***ε_(1,p)andΔε_(v)are negatively correlated with D,and CGBS with D=2.150 has the maximum deformation parameters(ε_(1,p)=0.05079,Δε_(v)=0.01990)due to the frictional slip effect caused by coarse *** increasing D,the failure modes of CGBSs are sequentially manifested as oblique shear failure,"Y-shaped"shear failure,and conjugate shear failure.

关键词： cemented gangue backfill materials particle size distribution true triaxial compression test carbon nanotubes mechanical properties failure modes

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Epidemiological investigation on mercury contamination in gold mining and its effect on health

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Journal of Environmental Sciences 2000年第S1期12卷 109-114页

In this paper, it is reported the environmental contamination of mercury in gold mining in China and the effect of mercury exposure on health. The results showed that Hg concentration of air in the Hg\|use workshop, the mining area and the population's living area were 0.058 mg/m 3, 0.047 mg/m 3 and 0.043 mg/m 3, respectively. Hg content of air in the suburbs 5 km from the mining area was 0.007 mg/m 3. The difference in Hg concentration of air was statistically significant ( p 0.05). All of the values determined above were excessed the Chinese national standards. Hg concentration of snowfall and soil in the mining area were 0.058 mg/L and 2.78 μg/g. Both of them differed from those of the living area ( pwenty\|five percent of workers in GI was higher than 4 ppm, and a small number of workers in this group was higher than 10 ppm. The Hg content of urine of eighty percent of workers in GI was higher than that of the Chinese normal human's standard. Some slight but typical clinical symptoms, of Hg poisoning were found in some worker's by clinical examination. It proved that the environment of the gold mining area was polluted by Hg vapor in varying degrees. The population w

关键词： gold mining mercury environment health

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储罐液位异常检测系统研究

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软件 2018年第3期39卷 32-36页

作者：齐秋平文必龙吴婷东北石油大学计算机与信息技术学院黑龙江大庆163318

目前,对于时序数据的异常检测方面有很多较为成熟的模型,但并没有一套基于储罐液位数据进行分析、预测以及异常检测的系统。油田上不同开采工程的数据大都来自测量仪表、液位仪、传感器,具有时序性的特点,为充分时序数据的价值,提出了... 详细信息

目前,对于时序数据的异常检测方面有很多较为成熟的模型,但并没有一套基于储罐液位数据进行分析、预测以及异常检测的系统。油田上不同开采工程的数据大都来自测量仪表、液位仪、传感器,具有时序性的特点,为充分时序数据的价值,提出了一套基于油田储罐液位数据的异常检测系统,用于检测储罐液位数据。该系统以对油田储油罐液位的特征提取、异常检测、模式识别、预测分析建立框架,并可将其推广到油田钻井、测井、录井、井下作业等业务的异常检测。

关键词：时序数据储罐液位异常检测特征提取预测分析

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Broadband and perfect terahertz absorber based on multilayer metamaterial with cross-ring patterned structures

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Optoelectronics Letters 2025年第1期21卷 1-6页

作者： wen Jin LIU Zhanzhi wu Zhengwei PAN Yu ZHANG Ying School of Science Xi'an Shiyou University Shaanxi Key Laboratory of Measurement and Control Technology for Oil and Gas Wells

Broadband and perfect terahertz absorber based on multilayer metamaterial using cross-ring patterned structures is proposed and investigated. The structure of the absorber is double absorption layers consisting of a chromium cross ring and eight isosceles right triangles. The unique structure of the double absorbing layers excites the electric dipole multimode resonance, giving rise to high absorption performance. Meanwhile, the influence of construal parameters on absorber behavior is also discussed. The numerical results show that the absorption achieves over 90% ranging from 2.45 THz to 6.25 THz and 99% absorption in the range of 3.7—5.3 THz. The realization of broadband and perfect absorber is described using the impedance matching principle. It is obviously found that the absorber is insensitive to the high angle of incidence for both transverse electric(TE) and transverse magnetic(TM) polarizations. Compared with the former reports, this absorber has remarkable improved absorption efficiency and smaller period. The terahertz absorber may be found applications in the fields of energy capture and thermal detection.

关键词： Metamaterials

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基于ANSYS的超薄扁平永磁直线电机的热设计研究

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机电产品开发与创新 2012年第1期25卷 52-54页

作者：毕文武范良志张楠武汉纺织大学机电工程学院湖北武汉430073

电机设计的重点在于其结构设计和热设计,而结构的制造工艺又与电机的散热系统的设计相关,所以电机的热设计研究是电机设计的重点和难点,论文针对一种自行设计的超薄扁平永磁直线电机,利用有限元软件ANSYS的热设计部分,解决了电机热设计... 详细信息

电机设计的重点在于其结构设计和热设计,而结构的制造工艺又与电机的散热系统的设计相关,所以电机的热设计研究是电机设计的重点和难点,论文针对一种自行设计的超薄扁平永磁直线电机,利用有限元软件ANSYS的热设计部分,解决了电机热设计数学模型的严重非线性化,不能直接得到收敛解的问题,并利用了ANSYS的APDL模块对电机有关热设计的参数进行了优化。

关键词：直线电机热设计 ANSYS APDL

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一种用于电织针的超薄直线电机磁路与结构设计方案的有限元分析

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武汉科技学院学报 2010年第4期23卷 1-3页

作者：范良志张楠毕文武武汉纺织大学机电工程学院湖北武汉430073

针对一种超薄永磁直线同步电机,在苛刻的尺寸约束条件下重点针对初级导线的布置、永磁的体积确定及布置方式进行研究,最后借助有限元分析软件ANSYS进行仿真,仿真结果能较好的满足电机的约束条件。分析计算表明,设计方案可行,为进一步开... 详细信息

针对一种超薄永磁直线同步电机,在苛刻的尺寸约束条件下重点针对初级导线的布置、永磁的体积确定及布置方式进行研究,最后借助有限元分析软件ANSYS进行仿真,仿真结果能较好的满足电机的约束条件。分析计算表明,设计方案可行,为进一步开展电机的实际试制工作打下了良好的理论基础。

关键词：永磁直线同步电机电机结构有限元 ANSYS

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