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Repairing whole facial nerve defects with xenogeneic acellular nerve grafts in rhesus monkeys

Neural Regeneration Research 2022年第5期17卷 1131-1137页

作者： Guo-Chen zhu Da-Jiang Xiao bi-wen zhu Yan Xiao Department of Otorhinolaryngology-Head and Neck Surgery Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical UniversityWuxiJiangsu ProvinceChina Department of Otorhinolaryngology-Head and Neck Surgery Affiliated Wuxi Clinical College of Nantong UniversityWuxiJiangsu ProvinceChina College of Animal Science&Technology Zhongkai University of Agriculture and EngineeringGuangzhouGuangdong ProvinceChina Department of Pathology Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical UniversityWuxiJiangsu ProvinceChina

Acellular nerve allografts conducted via chemical extraction have achieved satisfactory results in bridging whole facial nerve defects clinically,both in terms of branching a single trunk and in connecting multiple branches of an extratemporal segment.However,in the clinical treatment of facial nerve defects,allogeneic donors are limited.In this experiment,we exposed the left trunk and multiple branches of the extratemporal segment in six rhesus monkeys and dissected a gap of 25 mm to construct a monkey model of a whole left nerve defect.Six monkeys were randomly assigned to an autograft group or a xenogeneic acellular nerve graft group.In the autograft group,the 25-mm whole facial nerve defect was immediately bridged using an autogenous ipsilateral great auricular nerve,and in the xenogeneic acellular nerve graft group,this was done using a xenogeneic acellular nerve graft with trunk-branches.Examinations of facial symmetry,nerve-muscle electrophysiology,retrograde transport of labeled neuronal tracers,and morphology of the regenerated nerve and target muscle at 8 months postoperatively showed that the faces of the monkey appeared to be symmetrical in the static state and slightly asymmetrical during facial movement,and that they could actively close their eyelids completely.The degree of recovery from facial paralysis reached House-Brackmann grade II in both groups.Compound muscle action potentials were recorded and orbicularis oris muscles responded to electro-stimuli on the surgical side in each monkey.Fluoro Gold-labeled neurons could be detected in the facial nuclei on the injured side.Immunohistochemical staining showed abundant neurofilament-200-positive axons and soluble protein-100-positive Schwann cells in the regenerated nerves.A large number of mid-graft myelinated axons were observed via methylene blue staining and a transmission electron microscope.Taken together,our data indicate that xenogeneic acellular nerve grafts from minipigs are safe and effective for repairing whole facial nerve defects in

关键词： acellular nerve compound muscle action potentials facial nerve defect facial symmetry monkey nerve graft nerve regeneration retrograde labeling test synaptophysin xenogeneic

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UAV-assisted cooperative offloading energy efficiency system for mobile edge computing

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Digital Communications and Networks 2024年第1期10卷 16-24页

作者： Xue-Yong Yu wen-Jin Niu Ye zhu Hong-Bo zhu Jiangsu Key Laboratory of Wireless Communications Nanjing University of Posts and Telecommunications Nanjing210003China Engineering Research Centerof Health Service System Based on Ubiquitous Wireless Nework Ministyof EducationNanjng Universityof Posts and Telecommunication Nanjing210003China

Reliable communication and intensive computing power cannot be provided effectively by temporary hot spots in disaster areas and complex terrain ground infrastructure.Mitigating this has greatly developed the application and integration of UAV and Mobile Edge Computing(MEC)to the Internet of Things(loT).However,problems such as multi-user and huge data flow in large areas,which contradict the reality that a single UAV is constrained by limited computing power,still exist.Due to allowing UAV collaboration to accomplish complex tasks,cooperative task offloading between multiple UAVs must meet the interdependence of tasks and realize parallel processing,which reduces the computing power consumption and endurance pressure of terminals.Considering the computing requirements of the user terminal,delay constraint of a computing task,energy constraint,and safe distance of UAV,we constructed a UAV-Assisted cooperative offloading energy efficiency system for mobile edge computing to minimize user terminal energy consumption.However,the resulting optimization problem is originally nonconvex and thus,difficult to solve optimally.To tackle this problem,we developed an energy efficiency optimization algorithm using Block Coordinate Descent(BCD)that decomposes the problem into three convex subproblems.Furthermore,we jointly optimized the number of local computing tasks,number of computing offloaded tasks,trajectories of UAV,and offloading matching relationship between multi-UAVs and multiuser terminals.Simulation results show that the proposed approach is suitable for different channel conditions and significantly saves the user terminal energy consumption compared with other benchmark schemes.

关键词： Computation offloading Internet of things(IoT) Mobile edge computing(MEC) Block coordinate descent(BCD)

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ALKBH5 suppresses autophagic flux via N6-methyladenosine demethylation of ZKSCAN3 mRNA in acute pancreatitis

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World Journal of Gastroenterology 2024年第12期30卷 1764-1776页

作者： Tao Zhang Shuai zhu Geng-wen Huang Department of General Surgery Xiangya HospitalCentral South UniversityChangsha 410005HunanChina. National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangsha 410005HunanChina.

BACKGROUND Increasing evidence has demonstrated that N6-methyladenosine(m6A)RNA modification plays an essential role in a wide range of pathological conditions.Impaired autophagy is a critical hallmark of acute pancreatitis(AP).AIM To explore the role of the m6A modification of ZKSCAN3 in the regulation of autophagy in AP.METHODS The AP mouse cell model was established by cerulein-treated mouse pancreatic acinar cells(MPC-83),and the results were confirmed by the levels of amylase and inflammatory factors.Autophagy activity was evaluated by specific identification of the autophagy-related microstructure and the expression of autophagy-related genes.ZKSCAN3 and ALKBH5 were knocked down to study the function in AP.A m6A RNA binding protein immunoprecipitation assay was used to study how the m6A modification of ZKSCAN3 mRNA is regulated by ALKBH.RESULTS The increased expression of amylase and inflammatory factors in the supernatant and the accumulation of autophagic vacuoles verified that the AP mouse cell model was established.The downregulation of LAMP2 and upregulation of LC3-II/I and SQSTM1 demonstrated that autophagy was impaired in AP.The expression of ZKSCAN3 was upregulated in AP.Inhibition of ZKSCAN3 increased the expression of LAMP2 and decreased the expression of the inflammatory factors,LC3-II/I and SQSTM1.Furthermore,ALKBH5 was upregulated in AP.Knockdown of ALKBH5 downregulated ZKSCAN3 expression and restored decreased autophagic flux in AP.Notably,the bioinformatic analysis revealed 23 potential m6A modification sites on ZKSCAN3 mRNA.The m6A modification of ZKSCAN3 mRNA was significantly decreased in AP.Knockdown of ALKBH5 increased the modification of ZKSCAN3 mRNA,which confirmed that ALKBH5 upregulated ZKSCAN3 expression in a m6A-dependent manner.CONCLUSION ALKBH5 inhibits autophagic flux through m6A demethylation of ZKSCAN3 mRNA in AP,thereby aggravating the severity of the disease.

关键词： Acute pancreatitis Autophagy ZKSCAN3 N6-methyladenosine ALKBH5

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CdS/LaFeO_(3) heterojunctions with improved optical properties for visible-light catalytic applications

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Journal of Rare Earths 2024年第5期42卷 879-888,I0003页

作者： Jiaqi Wei wen zhu Xuelian Xu Xiao Xu Junjiang zhu Hubei Key Laboratory of biomass Fibers and Eco-dyeing&Finishing College of Chemistry and Chemical EngineeringWuhan Textile University ChemistryWuhan430200China

Construction of heterojunctions is a normal and effective strategy to improve the photocatalytic performances of semiconductors,through which both the lifetime and the redox ability of electrons/holes can be improved,as co mpared to the respective component.On this basis,we constructed Z-scheme CdS/LaFeO_(3) heterojunctions(CdS/LFO),by in-situ growing different amounts of CdS on the surface of LaFeO_(3),for photocatalytic degradation of tetracycline hydrochloride(TC)in aqueous solution at room temperature.The crystal structure,surface morphology and optoelectronic properties of the COS/LFO heterojunctions were systemically characterized to correlate the reaction activity.Photocatalytic tests indicate that the CdS/LFO heterojunctions exhibit promising activity for TC degradation under visible light irradiation(λ≥420 nm),with 85%TC conversion obtained at reaction time of 45 min,which is 3.4 and 1.9 times higher than that of CdS and LaFeO_(3).The CdS/LFO heterojunctions are also stable in the reaction and can be reused for four cycles with no appreciable activity loss.The applicability of CdS/LFO to photocatalytic degradation of organic dyes,as well as the reaction mechanism,was also explored.

关键词： LaFeO_(3) CdS Photocatalytic degradation Tetracycline hydrochloride Reaction mechanism Rare earths

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Divergent defluorocarboxylation of α-CF_(3) alkenes with formate via photocatalyzed selective mono-or triple C-F bond cleavage

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Science China Chemistry 2024年第1期67卷 368-373页

作者： Xing-Yu Wang Pei Xu wen-wen Liu Hao-Qiang Jiang Song-Lei zhu Dong Guo Xu zhu Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy School of PharmacyXuzhou Medical UniversityXuzhou 22100China

Unprecedented divergent synthesis of gem-difluorovinylacetic acid and glutaric acid derivatives fromα-CF_(3)alkenes with formate as the carbonyl source was disclosed.The reaction can undergo selective mono-or triple C-F bond cleavage by simply switching the photocatalyst and hydrogen atom transfer(HAT)catalyst under visible-light-induced conditions at room temperature.Foramte acts as both the C1 source and the reductant through the generation of CO_(2)^(·-)species,which underwent Giese radical addition to electron-deficient alkenes to trigger the consecutive C-F bond cleavage and carboxylation process.

关键词： C-F bond activation defluorocarboxylation carbon dioxide radical anion visible light

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Ferromagnetism and insulating behavior with a logarithmic temperature dependence of resistivity in Pb_(10-x)Cux(PO_(4))_(6)O

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Science China(Physics,Mechanics & Astronomy) 2024年第1期67卷 106-111页

作者： Yuhang Zhang Cong Liu Xiyu zhu Hai-Hu wen Center for Superconducting Physics and Materials National Laboratory of Solid State Microstructures and Department of PhysicsNational Collaborative Innovation Center of Advanced MicrostructuresNanjing UniversityNanjing 210093China

Recent claim of discovering above-room-temperature superconductivity(Tc≈400 K)at ambient pressure in copper doped apatite Pb10(PO_(4))_(6)O has stimulated world-wide enthusiasm and impulse of motion.A lot of follow-up works have been carried out with controversial conclusions.To check whether superconductivity is really present or absent in the material,we need samples which should have a rather pure phase of Pb_(10-x)Cux(PO_(4))_(6)O.Here we report the characterization results from the Pb_(10-x)Cux(PO_(4))_(6)O with a fraction of about 97 wt.%inferred from the fitting to the X-ray diffraction pattern,if assuming Cu2S as the only impurity.The resistivity measurements show that it is a semiconductor characterized roughly by a ln(1/T)temperature dependence in wide temperature region without trace of superconductivity.Magnetization measurements show that it has a general ferromagnetic signal with a weak superparamagnetic background.Many grains of the sample show clear interactions with a Nb Fe B magnet.The detected Cu concentration is much lower than the expected nominal one.Our results show the absence of metallicity and superconductivity in Pb_(10-x)Cux(PO_(4))_(6)O at ambient pressure,and suggest the presence of strong correlation effect.

关键词： superconductivity Pb10(PO4)6O ferromagnetic correlation effect

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Synergistic catalysis of the N-hydroxyphthalimide on flower-like bimetallic metal-organic frameworks for boosting oxidative desulfurization

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Petroleum Science 2024年第1期21卷 674-682页

作者： Jing He Kun zhu Wei Jiang Dong-Ao zhu Lin-Hua zhu Hai-Yan Huang wen-Shuai zhu Hua-Ming Li Institute for Energy Research Jiangsu UniversityZhenjiang212013JiangsuChina College of Chemistry and Chemical Engineering Hainan Normal UniversityKey Laboratory of Water Pollution Treatment and Resource Reuse of Hainan ProvinceHaikou571158HainanChina

Synergic catalytic effect between active sites and supports greatly determines the catalytic activity for the aerobic oxidative desulfurization of fuel oils.In this work,Ni-doped Co-based bimetallic metal-organic framework(CoNi-MOF)is fabricated to disperse N-hydroxyphthalimide(NHPI),in which the whole catalyst provides plentiful synergic catalytic effect to improve the performance of oxidative desulfurization(ODS).As a bimetallic MOF,the second metal Ni doping results in the flower-like morphology and the modification of electronic properties,which ensure the exposure of NHPI and strengthen the synergistic effect of the overall catalyst.Compared with the monometallic Co-MOF and naked NHPI,the NHPI@CoNi-MOF triggers the efficient activation of molecular oxygen and improves the ODS performance without an initiator.The sulfur removal of dibenzothiophene-based model oil reaches 96.4%over the NHPI@CoNi-MOF catalyst in 8 h of reaction.Furthermore,the catalytic product of this aerobic ODS reaction is sulfone,which is adsorbed on the catalyst surface due to the difference in polarity.This work provides new insight and strategy for the design of a strong synergic catalytic effect between NHPI and bimetallic supports toward high-activity aerobic ODS materials.

关键词： Metal-organic frameworks Doped bimetallic N-hydroxyphthalimide Aerobic processes Oxidative desulfurization

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Solvent-free carbon sphere nanofluids towards intelligent lubrication regulation

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Friction 2024年第1期12卷 95-109页

作者： Sheng ZHANG wen LI Xiaoliang MA Xiaoqiang FAN Minhao zhu Tribology Research Institute School of Mechanical EngineeringSouthwest Jiaotong UniversityChengdu 610031China Key Laboratory of Advanced Technologies of Materials Ministry of EducationSchool of Materials Science and EngineeringSouthwest Jiaotong UniversityChengdu 610031China

By simply switching the electrical circuit installed on steel/steel contact,the tribological behaviors of nanofluids(NFs)can be regulated in real time,thereby achieving the desired performance of friction reduction and wear resistance.Herein,solvent-free carbon spherical nanofluids(C-NFs)were successfully prepared for intelligent lubrication regulation.C-NFs with excellent lubrication performance can immediately reduce the coefficient of friction(COF)despite applying a weak electric potential(1.5 V).Moreover,polyethylene glycol 400(PEG400)containing 5.0 wt%C-NFs remained responsive to electrical stimulation under the intermittent voltage application with an average coefficient of friction(ACOF)reduction of 20.8%over PEG400.Such intelligent lubrication regulation of C-NFs under an external electric field(EEF)mainly depends on the orderly arranged double-electric adsorption film of ion canopy-adsorbed carbon spheres(CSs).The intermittent electrical application can continuously reinforce the adsorption film and its durability for real-time controlling the sliding interfaces.Electrical-stimulation-responsive intelligent lubricants provide a new technical support for realizing intelligent stepless control of devices.

关键词： solvent-free carbon spherical nanofluids(C-NFs) intelligent lubrication regulation lubrication performance adsorption film

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Advances in the differentiation of pluripotent stem cells into vascular cells

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World Journal of Stem Cells 2024年第2期16卷 137-150页

作者： Yi-Chang Jiao Ying-Xin Wang wen-zhu Liu Jing-wen Xu Yu-Ying Zhao Chuan-zhu Yan Fu-Chen Liu Department of Neurology Qilu Hospital of Shandong UniversityJinan 250012Shandong ProvinceChina Research Institute of Neuromuscular and Neurodegenerative Diseases Qilu Hospital of Shandong UniversityJinan 250012Shandong ProvinceChina Mitochondrial Medicine Laboratory Qilu Hospital(Qingdao)of Shandong UniversityQingdao 266103Shandong ProvinceChina Brain Science Research Institute Shandong UniversityJinan 250012Shandong ProvinceChina

Blood vessels constitute a closed pipe system distributed throughout the body,transporting blood from the heart to other organs and delivering metabolic waste products back to the lungs and kidneys.Changes in blood vessels are related to many disorders like stroke,myocardial infarction,aneurysm,and diabetes,which are important causes of death worldwide.Translational research for new appro-aches to disease modeling and effective treatment is needed due to the huge socio-economic burden on healthcare systems.Although mice or rats have been widely used,applying data from animal studies to human-specific vascular physiology and pathology is difficult.The rise of induced pluripotent stem cells(iPSCs)provides a reliable in vitro resource for disease modeling,regenerative medicine,and drug discovery because they carry all human genetic information and have the ability to directionally differentiate into any type of human cells.This review summarizes the latest progress from the establishment of iPSCs,the strategies for differentiating iPSCs into vascular cells,and the in vivo trans-plantation of these vascular derivatives.It also introduces the application of these technologies in disease modeling,drug screening,and regenerative medicine.Additionally,the application of high-tech tools,such as omics analysis and high-throughput sequencing,in this field is reviewed.

关键词： Induced pluripotent stem cell Blood vessels Vascular organoids Endothelial cells Smooth muscle cells Pericytes Tissue engineering vascular graft

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Abnormal function of EPHA2/p.R957P mutant in congenital cataract

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International Journal of Ophthalmology(English edition) 2024年第6期17卷 1007-1017页

作者： Jing-Jin Zhang Zong-Fu Cao bi-Ting Zhou Ju-Hua Yang Zhong Li Shuang Lin Xiao-Le Chen Nan-wen Zhang Qin Ye Xu Ma Yi-Hua zhu Department of Ophthalmology the First Affiliated Hospital of Fujian Medical UniversityFujian Institute of OphthalmologyFujian Provincial Clinical Medical Research Center of Eye Diseases and OptometryFuzhou 350005Fujian ProvinceChina Department of Ophthalmology National Regional Medical CenterBinhai Campus of the First Affiliated HospitalFujian Medical UniversityFuzhou 350005Fujian ProvinceChina Chinese Academy of Medical Sciences&Peking Union Medical College Beijing 100000China National Human Genetic Resources Center National Research Institute for Family PlanningBeijing 100081China Department of bioengineering and biopharmaceutics School of PharmacyFujian Medical UniversityFuzhou 350004Fujian ProvinceChina

AIM:To identify genetic defects in a Chinese family with congenital posterior polar cataracts and assess the pathogenicity.METHODS:A four-generation Chinese family affected with autosomal dominant congenital cataract was recruited.Nineteen individuals took part in this study including 5 affected and 14 unaffected individuals.Sanger sequencing targeted hot-spot regions of 27 congenital cataract-causing genes for variant discovery.The pathogenicity of the variant was evaluated by the guidelines of American College of Medical Genetics and InterVar software.Confocal microscopy was applied to detect the subcellular localization of fluorescence-labeled ephrin type-A receptor 2(EPHA2).Co-immunoprecipitation assay was implemented to estimate the interaction between EphA2 and other lens membrane proteins.The mRNA and protein expression were analyzed by reverse transcription-polymerase chain reaction(qRT-PCR)and Western blotting assay,respectively.The cell migration was analyzed by wound healing assay.Zebrafish model was generated by ectopic expression of human EPHA2/p.R957P mutant to demonstrate whether the mutant could cause lens opacity in vivo.RESULTS:A novel missense and pathogenic variant c.2870G>C was identified in the sterile alpha motif(SAM)domain of EPHA2.Functional studies demonstrated the variant’s impact:reduced EPHA2 protein expression,altered subcellular localization,and disrupted interactions with other lens membrane proteins.This mutant notably enhanced human lens epithelial cell migration,and induced a central cloudy region and roughness in zebrafish lenses with ectopic expression of human EPHA2/p.R957P mutant under differential interference contrast(DIC)optics.CONCLUSION:Novel pathogenic c.2870G>C variant of EPHA2 in a Chinese congenital cataract family contributes to disease pathogenesis.

关键词： congenital cataract EPHA2 missense variant function analysis

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