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Lipase and photodecarboxylase coexpression: A potential strategy for alkane-based biodiesel production from natural triglycerides

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Chinese Journal of Chemical Engineering 2024年第3期67卷 238-246页

作者： Yong-Yi Zeng Xin-Yi Xu Jin-Xuan Xie Wen-Li chen Lan Liu Xin-Jian Yin bi-shuang chen School of Marine Sciences Sun Yat-Sen UniversityZhuhai 519080China Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai) Zhuhai 519080China

Alkane-based biodiesel is considered the next generation of biodiesel owing to its potential environmental benefits and the fact that it exhibits much higher specific caloric values than traditional ***,the formidable obstacle impeding the commercialization of this cutting-edge fuel alternative lies in the cost associated with its *** this study,an engineered strain Escherichia coli(***)showcasing harmonized coexpression of a lipase(from Thermomyces lanuginosus lipase,TLL)and a fatty acid photodecarboxylase(from Chlorella variabilis,CvFAP)was first constructed to transform triglycerides into *** potential of *** BL21(DE3)/pRSFDuet-1-TLL-CvFAP for alkane synthesis was evaluated with tripalmitin as a model substrate under various process *** a comprehensive examination of the reaction parameters,the scope of the biotransformation was expanded to‘real’substrates(vegetable oils).The results showed that bioderived oils can be transformed into alkanes with high yields(0.80-10.20 mmol·L^(-1))under mild conditions(35℃,pH 8.0,and 36 h)and blue light *** selected processes were performed on an increased lab scale(up to 100 ml)with up to 24.77 mmol·L^(-1) tripalmitin,leading to a yield of 18.89 mmol·L^(-1) *** the employment of a method for efficiently producing alkanes under mild conditions and a simple procedure to isolate alkanes from the reaction system,the utilization of sustainable biomass as a fundamental feedstock emerges as the primary solution to lower the cost of alkane-based ***,this study proposes a readily implementable and highly effective approach for alkane-based biodiesel production.

关键词： Alkane-based biodiesel Renewable biomass Co-overexpression Lipase Photodecarboxylase

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Engineering atomic Rb-N configurations to tune radical pathways for highly selective photocatalytic H2O2 synthesis coupled with biomass valorization

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Journal of Energy Chemistry 2025年第1期100卷 215-225页

作者： Jiaye Li shuang Pan Yihuang chen Qiong Liu College of Chemistry and Materials Engineering Wenzhou University Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology (China) Institute of Analysis Guangdong Academy of Sciences (China National Analytical CenterGuangzhou)

Photocatalytic oxygen reduction for hydrogen peroxide （H2O2） synthesis presents a green and costeffective production ***,achieving highly selective H2O2synthesis remains challenging,necessitating precise control over free radical reaction pathways and minimizing undesirable oxidative ***,we report for the visible light-driven simultaneous co-photocatalytic reduction of O2to H2O2and oxidation of biomass using the atomic rubidium-nitride modified carbon nitride（CNRb）.The optimized CNRb catalyst demonstrates a record photoreduction rate of 8.01 mM h-1for H2O2generation and photooxidation rate of 3.75 mM h-1for furfuryl alcohol to furoic acid,achieving a remarkable solar-to-chemical conversion （SCC） efficiency of up to 2.27%.Experimental characterizations and DFT calculation disclosed that the introducing atomic Rb–N configurations allows for the high-selective generation of superoxide radicals while suppressing hydroxyl free radical *** is because the Rb–N serves as the new alternative site to perceive a stronger connection position for O2adsorption and reinforce the capability to extract protons,thereby triggering a high selective redox product *** study holds great potential in precisely regulating reactive radical processes at the atomic level,thereby paving the way for efficient synthesis of H2O2coupled with biomass valorization.

关键词： Atomic economy Radical pathway regulation Rubidium atom coordination biomass valorization

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Cortico-striatal gamma oscillations are modulated by dopamine D3 receptors in dyskinetic rats

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Neural Regeneration Research 2025年第4期20卷 1164-1177页

作者： Pengfei Wang Yuewei bi Min Li Jiazhi chen Zhuyong Wang Huantao Wen Ming Zhou Minjie Luo Wangming Zhang Neurosurgery Center Department of Pediatric NeurosurgeryGuangdong Provincial Key Laboratory on Brain Function Repair and RegenerationZhujiang HospitalSouthern Medical UniversityGuangzhouGuangdong ProvinceChina

Long-term levodopa administration can lead to the development of levodopa-induced *** oscillations are a widely recognized hallmark of abnormal neural electrical activity in levodopa-induced ***,studies have reported increased oscillation power in cases of levodopa-induced ***,little is known about how the other electrophysiological parameters of gamma oscillations are altered in levodopa-induced ***,the role of the dopamine D3 receptor,which is implicated in levodopa-induced dyskinesia,in movement disorder-related changes in neural oscillations is *** found that the cortico-striatal functional connectivity of beta oscillations was enhanced in a model of Parkinson’s ***,levodopa application enhanced cortical gamma oscillations in cortico-striatal projections and cortical gamma aperiodic components,as well as bidirectional primary motor cortex(M1)↔dorsolateral striatum gamma *** of PD128907(a selective dopamine D3 receptor agonist)induced dyskinesia and excessive gamma oscillations with a bidirectional M1↔dorsolateral striatum ***,administration of PG01037(a selective dopamine D3 receptor antagonist)attenuated dyskinesia,suppressed gamma oscillations and cortical gamma aperiodic components,and decreased gamma causality in the M1→dorsolateral striatum *** findings suggest that the dopamine D3 receptor plays a role in dyskinesia-related oscillatory activity,and that it has potential as a therapeutic target for levodopa-induced dyskinesia.

关键词： aperiodic components dopamine D3 receptor dorsolateral striatum functional connectivity gamma oscillations levodopa-induced-dyskinesia local field potentials neuromodulation Parkinson’s disease primary motor cortex

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Value of endoscopic methylene blue and Lugol's iodine double staining and detection of GST-n and telomerase in the early diagnosis of esophageal carcinoma

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World Journal of Gastroenterology 2005年第39期11卷 6090-6095页

作者： Xuan Zhu shuang-Hong Zhang Kun-He Zhang bi-Ming Li Jiang chen Department of Gastroenterology the First Affiliated Hospital Jiangxi Medical College Nanchang 330006 Jiangxi Province China Department of Digestive Institution the First Affiliated Hospital Jiangxi Medical College Nanchang 330006 Jiangxi Province China

AIM：To explore the expressions of GST-π and telomerase activity in esophageal carcinoma and premalignant lesions and to investigate the value of endoscopic methylene blue （MB） and Lugol＇s iodine double staining. METHODS： Seventy-two patients with esophagopathy were sprayed endoscopically with MB and Lugol＇s iodine in proper order and the areas stained blue and brown, and the area between the blue and brown stains were obtained. Depending on the pattern of mucosal staining, biopsy specimen was obtained. GST-π and telomerase activity in specimens were examined by immunohistochemistry and PCR-based silver staining telomeric repeat amplification protocol, respectively. RESULTS： After MB and Lugol＇s iodine staining, the area between both the colors was obtained in 64 of the 72 patients and the areas were stained blue and brown in all of the 72 patients. Association test of two simultaneous ordinal categorical data showed a correlation between the esophageal mucosal staining and the esophageal histology （P〈0.005）. The expression of GST-π and telomerase activity in esophageal carcinoma and premalignant lesions increased. The expression of GST-π and telomerase activity in dysplasia and carcinoma was significantly higher than that in normal epithelium （P〈0.005）. The expression in hyperplasia was slightly higher than that in normal epithelium. With the lesions progressing from low- to moderate- to high-grade dysplasia, the positive rate increased （P〈0.025）. Expression of GST-π was correlated with that of telomerase activity in dysplasia and carcinoma （φ= 0.4831, P〈0.005;φ= 0.3031, P〈0.025, respectively）; but there was no correlation between them in normal epithelium and hyperplasia. CONCLUSION： The expression of GST-π and telomerase may be an early event in the carcinogenesis of esophagus. They may play an induced and synergistic role with each other in the carcinogenesis of esophagus. Endoscopic MB and Lugol＇s iodine double staining and detection of GST-π and telomerase acti

关键词： Esophageal carcinoma Endoscopy Glutathione S-transferases Telomerase

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Combination of Organic and Inorganic Electrolytes for Composite Membranes Toward Applicable Solid Lithium Batteries

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Chemical Research in Chinese Universities 2021年第2期37卷 246-253页

作者： MU shuang bi Zhijie GAO Shenghan GUO Xiangxins College of Physics Qingdao UniversityQingdao266071P.R.China

To meet the demand for long-range electric vehicles with high-energy-density batteries,the solid-state batteries(SSBs)have attracted ever-increasing attention due to their enormous potential in affording the energy density greater than 400 W·h/*** the key materials,the solid electrolytes can be classified as inorganic electrolyte and organic *** former usually has high ionic conductivity,good stability and mechanical properties,whereas being heavy and *** latter is usually flexible,light and easy to mass produce,nevertheless has poor ionic conductivity and ***,the combination of the organic and the inorganic electrolytes for the composite membranes has become the inevitable trend to achieve the high energy density and safety of lithium *** the perspective of practical application,this paper discusses how to construct the ideal organic-inorganic composite solid electrolyte with low areal specific resistance,thin texture,wide electrochemical window and high safety for applicable ***,the critical challenges and future development directions are prospected for the composite solid electrolytes.

关键词： Composite electrolyte Solid-state lithium battery Energy density Security

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Outer synchronization of uncertain small-world networks via adaptive sliding mode control

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Applied Mathematics and Mechanics(English Edition) 2015年第3期36卷 319-328页

作者： shuang LIU Liqun chen Shanghai Institute of Applied Mathematics and Mechanics Shanghai University Department of Mechanics Shanghai University Shanghai Key Laboratory of Mechanics in Energy Engineering Shanghai University

The outer synchronization of irregular coupled complex networks is inves- tigated with nonidentical topological structures. The switching gain is estimated by an adaptive technique, and a sliding mode controller is designed to satisfy the sliding con- dition. The outer synchronization between two irregular coupled complex networks with different initial conditions is implemented via the designed controllers with the corre- sponding parameter update laws. The chaos synchronization of two small-world networks consisting of N uncertain identical Lorenz systems is achieved to demonstrate the appli- cations of the proposed approach.

关键词： adaptive synchronization sliding mode control Lyapunov stability small- world network

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Possible Streams of the Globular Clusters in the Galaxy

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Chinese Journal of Astronomy and Astrophysics 2007年第1期7卷 111-119页

作者： shuang Gao bi-Wei Jiang Yong-Heng Zhao Department of Astronomy Beijing Normal University Beijing 100875 National Astronomical Observatories Chinese Academy of Sciences Beijing 100012

We aim to retrieve ghost streams under the assumption that all the globular clusters in our Galaxy were formed in very early merge events. Our results are based on two speculations： that the specific energy and angular momentum of the globular clusters after merge are not changed in the course of evolution and that the globular clusters with a common origin would stay in the same orbit plane as the parent galaxy. After taking into account the apogalacticum distance of the orbits, we suggest with some Confidence five possible streams. The number of streams is consistent with the previous results. Three of the four well established members of the Sagittadus stream were found to be in one of our streams. Several other globular clusters in our result were also thought to come from accretion by previous researchers. The orbital parameters of the streams are derived, which provide a way to test whether these streams are true with the help of more accurate measurement of proper motions of the globular clusters.

关键词： globular clusters general-Galaxy formation-Galaxy halo

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Development of a seven-cell S-band standing-wave RF-deflecting cavity for Tsinghua Thomson scattering X-ray source

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Nuclear Science and Techniques 2021年第4期32卷 20-29页

作者： Xian-Cai Lin Hao Zha Jia-Ru Shi Liu-Yuan Zhou shuang Liu Jian Gao Huai-bi chen Department of Engineering Physics Tsinghua UniversityBeijing 100084China Key Laboratory of Particle and Radiation Imaging(Tsinghua University) Ministry of EducationBeijing 100084China

A 2856-MHz,π-mode,seven-cell standingwave deflecting cavity was designed and fabricated for bunch length measurement in Tsinghua Thomson scattering X-ray source(TTX)*** cavity was installed in the TTX and provided a deflecting voltage of 4.2 MV with an input power of 2.5 *** length diagnoses of electron beams with energies up to 39 MeV have been *** this article,the RF design of the cavity using HFSS,fabrication,and RF test processes are ***-power operation with accelerated beams and calibration of the deflecting voltage are also presented.

关键词： Deflecting RF cavity Standing wave Bunchlength measurement Thomson X-ray source

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A critical review of ferritin as a drug nanocarrier:Structure,properties,comparative advantages and challenges

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Particuology 2022年第5期20卷 65-84页

作者： shuang Yin Kenneth Davey Sheng Dai Yongdong Liu Jingxiu bi School of Chemical Engineering&Advanced Materials Faculty of EngineeringComputer and Mathematical Sciences.University of AdelaideAdelaideAustralia Department of Chemical Engineering Brunei University LondonUxbridge UB83PHUnited Kingdom State Key Laboratory of biochemistry Engineering.Institute of Process Engineering Chinese Academy of Sciences.BeijingChina

Ferritin stores and releases iron ions in *** is globally important as a drug *** is because of its unique hollow-spherical structure,desirable stability and biological *** drug-loading approaches plus various functionalization approaches have been developed to improve ferritin in response to differing demands in disease ***,we critically review ferritin drug delivery and evaluate its diverse drug-loading and functionalization approaches,we:(1)Introduce basic structural and property information related to ferritin as a drug nanocarrier;(2)Contrast in detail the different means to load drugs and the selection of drug loading means;(3)Discuss multiple ferritin functionalization approaches,together with related advantages and potential risks;and,(4)Compare ferritin with alternative,commonly-used drug *** conclude that despite that no drugs based on ferritin are commercially available,the market potential for it is significant,and evaluate future research *** from this work will be of immediate benefit and interest to a wide range of researchers and manufacturers for drug delivery using ferritin.

关键词： Ferritin Drug delivery Drug nanocarrier Protein functionalization

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Application and efficacy of reconstructing forked corpus spongiosum in distal/midshaft hypospadias repair

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Asian Journal of Andrology 2021年第1期23卷 47-51页

作者： bin Zhang Yun-Li bi shuang-Sui Ruan Department of Pediatric Urology Children's Hospital of Fudan UniversityShanghai 201102China

We reviewed our experience in reconstructing forked corpus spongiosum(FCS)in distal/midshaft hypospadias repair and analyzed the efficacy of this surgical *** August 2013 to December 2018,137 consecutive cases of distal/midshaft hypospadias operated by the same surgeon in Urology Department,Children’s Hospital of Fudan University(Shanghai,China),were retrospectively ***-four patients who underwent routine tubularized incised plate(TIP)or onlay island flap(ONLAY)surgery were included in the nonreconstructing group,and 73 patients who underwent reconstructing FCS during TIP or ONLAY surgery were included as the reconstructing ***-eight cases underwent TIP,and 26 underwent ONLAY in the nonreconstructing group,with a median follow-up of 44(range:30-70)***-seven cases underwent TIP,and 46 underwent ONLAY in the reconstructing group,with a median follow-up of 15(range:6-27)*** the nonreconstructing/reconstructing groups,the mean age at the time of surgery was 37.55(standard deviation[s.d.]:29.65)/35.23(s.d.:31.27)months,the mean operation duration was 91.95(s.d.:12.17)/93.84(s.d.:14.91)min,the mean neourethral length was 1.88(s.d.:0.53)/1.94(s.d.:0.53)cm,and the mean glans width was 11.83(s.d.:1.32)/11.56(s.d.:1.83)***(18.8%)/5(6.8%)postoperative complications occurred in the nonreconstructing/reconstructing *** included fistula(5/2),glans dehiscence(3/0),diverticulum(1/2),residual chordee(3/0),and meatus stenosis(0/1)in each *** was a significant difference in the overall rate of complications(P=0.035).These results indicate that the technique of reconstructing FCS provides excellent outcomes with fewer complications in distal/midshaft hypospadias repair.

关键词： complications distal/midshaft hypospadias forked corpus spongiosum glans tilt

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