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Efficient Humoral and Cellular Immune Responses Induced by a Chimeric Virus-like Particle Displaying the Epitope of EV71 without Adjuvant

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biomedical and Environmental Sciences 2018年第5期31卷 343-350页

作者： liANG Pu YI Yao SU Qiu Dong QIU Feng FAN Xue Ting LU Xue Xin bi sheng li National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 102206China Institute of Infectious Diseases Beijing Ditan HospitalCapital Medical UniversityBeijing Key Laboratory of Emerging Infectious DiseasesBeijing 100015China

Objective To eliminate the side effects of aluminum adjuvant and His-tag,we constructed chimeric VLPs displaying the epitope of EV71（SP70） without *** evaluating whether the VLPs could efficiently evoke not only humoral but also cellular immune responses against EV71 without *** The fusion protein was constructed by inserting SP70 into the MIR of truncated HBc Ag sequence,expressed in ***,and purified through ion exchange chromatography and density gradient *** were immunized with the VLPs and sera were collected *** specific antibody titers,Ig G subtypes and neutralizing efficacy were detected by EliSA,neutralization assay,and EV71 lethal ***-γ and IL-4 secreted by splenocytes were tested by EliSPOT *** HBc-SP70 proteins can self-assemble into empty *** immunization with HBc-SP70 VLPs,the detectable anti-EV71 antibodies were effective in neutralizing EV71 and protected newborn mice from EV71 lethal *** was no significant difference for the immune efficacy whether the aluminum adjuvant was added or *** specific Ig G subtypes were mainly IgG1 and IgG2 b and splenocytes from the mice immunized produced high levels of IFN-γ and *** The fusion proteins without His-tagged was expressed and purified as soluble chimeric HBc-SP70 VLPs without *** the absence of adjuvant,they were efficient to elicit high levels of Th1/Th2 mixed immune response as well as assisted by aluminum ***,the chimeric VLPs have potential to prevent HBV and EV71 infection simultaneously.

关键词： Virus-like particles Enterovirus 71 Neutralizing antibody Humoral and cellular immunity Adjuvant Vaccine

Constant charge method or constant potential method:Which is better for molecular modeling of electrical double layers?

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Journal of Energy Chemistry 2024年第7期94卷 54-60页

作者： liang Zeng Xi Tan Xiangyu Ji Shiqi li Jinkai Zhang Jiaxing Peng sheng bi Guang Feng State Key Laboratory of Coal Combustion Schoolof Energy and PowerEngineeringHuazhong Universityof Science and Technology(HUST)Wuhan 430074HubeiChina Sorbonne Universite CNRSPhysico-chimie des Electrolytes et Nanosystemes InterfaciauxPHENIXF-75005 ParisFrance

In molecular modeling of electrical double layers(EDLs),the constant charge method(CCM)is prized for its computational efficiency but cannot maintain electrode equipotentiality like the more resourceintensive constant potential method(CPM),potentially leading to *** certain scenarios,CCM can yield results identical to ***,there are no clear guidelines to determine when CCM is sufficient and when CPM is ***,we conduct a series of molecular simulations across various electrodes and electrolytes to present a comprehensive comparison between CCM and CPM under different charging *** reveal that CCM approximates CPM effectively in capturing equilibrium EDL and current-driven dynamics in open electrode systems featuring ionic liquids or regular concentration aqueous electrolytes,while CPM is indispensable in scenarios involving organic and highly concentrated aqueous electrolytes,nanoconfinement effects,and voltage-driven *** work helps to select appropriate methods for modeling EDL systems,prioritizing accuracy while considering computationalefficiency.

关键词： Electrochemical interface Molecular dynamics Electrode polarization modeling Nanoconfinement effect

Recent Advances in Deciphering the Mechanisms and biological Functions of DNA Demethylation

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Chinese Journal of Chemistry 2024年第6期42卷 645-651页

作者： Yang Feng sheng-Jun Chen bi-Feng Yuan Key Laboratory of Aquatic Product Processing Ministry of Agriculture and Rural AffairsNational R&D Center for Aquatic Product ProcessingSouth China Sea Fisheries Research InstituteChinese Academy of Fishery SciencesGuangzhouGuangdong510300 China School of Public Health Research Center of Public HealthRenmin Hospital of Wuhan UniversityWuhan UniversityWuhanHubei430071 China

5-Methylcytosine (5mC) is a dynamic and reversible epigenetic modification in genomic DNA of higher *** has been well-established that the demethylation of 5mC occurs through the ten-eleven translocation (TET)-mediated oxidation of 5mC followed by thymine DNA glycosylase (TDG)-initiated base excision repair (BER).Recent findings also have identified an alternative pathway of DNA *** this pathway,TET enzymes directly oxidize 5mC to form 5-formylcytosine (5fC) or 5-carboxylcytosine (5caC).These modified bases can undergo direct deformylation or decarboxylation,***,DNA demethylation can also occur through the deamination of 5mC and 5hmC,resulting in the production of thymine and 5-hydroxymethyluracil (5hmU),*** DNA demethylation pathways possess critical functional implications and roles in biological *** Recent Advances article will focus on the studies of mechanisms and biological functions of DNA demethylation,shedding light on the reversible nature of the epigenetic modification of 5mC.

关键词： Epigenetics DNA DNA methylation 5-Methylcytosine Demethylation Mechanisms biological functions C—C bond cleavage Mass spectrometry

Early changes in corneal densitometry after FS-LASIK combined with accelerated corneal cross-linking for correction of high myopia

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International Journal of Ophthalmology(English edition) 2024年第7期17卷 1307-1312页

作者： Qing-Bao Wang Hong-sheng bi Xiao-Fan Wang Hua Fan li li Peng Ji Shandong University of Traditional Chinese Medicine Jinan 250014Shandong ProvinceChina Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine Shandong Clinical Medicine Research CenterJinan 250002Shandong ProvinceChina Shandong Academy of Eye Disease Prevention and Therapy Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular DiseasesJinan 250002Shandong ProvinceChina

AIM:To observe the effects of femtosecond laserassisted excimer laser in situ keratomileusis combined with accelerated corneal cross-linking(FS-LASIK Xtra)on corneal densitometry after correcting for high ***:In this prospectively study,130 patients underwent FS-LASIK or FS-LASIK Xtra for high *** right eyes were selected for inclusion in the study,of which 65 cases of 65 eyes in the FS-LASIK group,65 patients with 65 eyes in the FS-LASIK Xtra *** were evaluated for corneal densitometry at 1,3,and 6mo postoperatively using Pentacam Scheimpflug ***:Preoperative differences in corneal densitometry between the FS-LASIK and FS-LASIK Xtra groups in different ranges were not statistically significant(P>0.05).Layer-by-layer analysis revealed statistically significant differences in the anterior(120μm),central,and total layer corneal densitometry between the FS-LASIK and FS-LASIK Xtra groups at 1 and 3mo postoperatively(all P0.05).CONCLUSION:There is an increase in internal corneal densitometry during the early postoperative period after FS-LASIK Xtra for correction of high ***,the densitometry values decreased to the level of conventional FS-LASIK at 6mo after surgery,with the most significant changes observed in the superficial central zone.

关键词： femtosecond laser accelerated corneal cross-linking corneal densitometry high myopia femtosecond laser in situ keratomileusis

Long Non-coding RNA PCED1B Antisense RNA 1 Promotes Cell Proliferation and Invasion in Hepatocellular Carcinoma by Regulating the MicroRNA-34a/CD44 Axis

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Current Medical Science 2024年第3期44卷 503-511页

作者： Jian-gang bi Qi li Yu-sheng GUO li-ping liU Shi-yun BAO Ping XU Department of Hepatobiliary and Pancreatic Surgery Shenzhen People's HospitalThe Second Clinical Medical CollegeJinan UniversityThe First Affiliated HospitalSouthern University of Science and TechnologyShenzhen 518020China Department of Endocrinology Shenzhen People's HospitalThe Second Clinical Medical CollegeJinan UniversityThe First Affiliated HospitalSouthern University of Science and TechnologyShenzhen 518020China

Objective This study aimed to examine the role of long non-coding RNA PCED1B antisense RNA 1(PCED1B-AS1)in the development of hepatocellular carcinoma(HCC).Methods A total of 62 pairs of HCC tissues and adjacent non-tumor tissues were obtained from 62 HCC *** interactions of PCED1B-AS1 and microRNA-34a(miR-34a)were detected by dual luciferase activity assay and RNA pull-down *** RNA expression levels of PCED1B-AS1,miR-34a and CD44 were detected by RT-qPCR,and the protein expression level of CD44 was determined by Western *** cell proliferation was detected by cell proliferation assay,and the cell invasion and migration by transwell invasion *** HCC tumor growth after PCED1B-AS1 was downregulated was determined by in vivo animal *** PCED1B-AS1 was highly expressed in HCC tissues,which was associated with poor survival of HCC ***,PCED1B-AS1 interacted with miR-34a in HCC cells,but they did not regulate the expression of each ***,PCED1B-AS1 increased the expression level of CD44,which was targeted by *** cell proliferation and invasion assay revealed that miR-34a inhibited the proliferation and invasion of HCC in vitro,while CD44 exhibited the opposite ***,PCED1B-AS1 suppressed the role of ***,the knockdown of PCED1B-AS1 repressed the HCC tumor growth in nude mice in *** PCED1B-AS1 may play an oncogenic role by regulating the miR-34a/CD44 axis in HCC.

关键词： long non-coding RNA PCED1B antisense RNA 1(PCED1B-AS1) hepatocellular carcinoma microRNA-34a(miR-34a) CD44 proliferation invasion

Intranasal Immunization Using CTA1-DD as a Mucosal Adjuvant for an Inactivated Influenza Vaccine

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biomedical and Environmental Sciences 2019年第7期32卷 531-540页

作者： FAN Xue Ting WANG Yun Long SU Qiu Dong QIU Feng YI Yao JIA Zhi Yuan WANG Da Yan QIN Kun ZOU Ye Ning bi sheng li SHEN li Ping National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China Henan bioengineering Research Center Zhengzhou 4500466 Henan China Sinovac biotech Co. Ltd. Beijing 100085 China

Objective To evaluate the effect of intranasal immunization with CTA1-DD as mucosal adjuvant combined with H3N2 split vaccine. Methods Mice were immunized intranasally with PBS(negative control), or H3N2 split vaccine(3 μg/mouse) alone, or CTA1-DD(5 μg/mouse) alone, or H3N2 split vaccine(3 μg/mouse) plus CTA1-DD(5 μg/mouse). Positive control mice were immunized intramuscularly with H3N2 split vaccine(3 μg/mouse) and alum adjuvant. All the mice were immunized twice, two weeks apart. Then sera and mucosal lavages were collected. The specific HI titers, IgM, IgG, IgA, and IgG subtypes were examined by EliSA. IFN-γ and IL-4 were test by EliSpot. In addition, two weeks after the last immunization, surivival after H3N2 virus lethal challenge was measured. Results H3N2 split vaccine formulated with CTA1-DD could elicit higher Ig M, Ig G and hemagglutination inhibition titers in sera. Furthermore, using CTA1-DD as adjuvant significantly improved mucosal secretory Ig A titers in bronchoalveolar lavages and vaginal lavages. Meanwhile this mucosal adjuvant could enhance Th-1-type responses and induce protective hemagglutination inhibition titers. Notably, the addition of CTA1-DD to split vaccine provided 100% protection against lethal infection by the H3N2 virus. Conclusion CTA1-DD could promote mucosal, humoral and cell-mediated immune responses, which supports the further development of CTA1-DD as a mucosal adjuvant for mucosal vaccines.

关键词： Adjuvants H3N2 Influenza Mucosal immunization Split vaccine

Expression and Purification of Recombinant Hepatitis Delta Virus(HDV) Antigen for Use in a Diagnostic EliSA for HDV Infection Using the High-Density Fermentation Strategy in Escherichia coli

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biomedical and Environmental Sciences 2016年第6期29卷 417-423页

作者： LU Xue Xin YI Yao SU Qiu Dong bi sheng li National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention

Objective Hepatitis Delt a Virus（HDV） antigen is widely used as a capture antigen in EliSAs for the identification of HDV infection; large amounts of recombinant HDV antigen with active antigenicity are required for this purpose. Methods Reconstruct the gene of HDV antigen based on the bias code of Escherichia coli, the recombinant protein expresses by high-density fermentation with fed-batch feeding strategy, and purify by immobilized metal chromatography. The sensitivity and specificity of this antigen detect by EliSA method. Results The expression of HDV antigen can reach 20% of the total cell mass in the soluble form. The recombinant HDV antigen can be conveniently purified（98%） by immobilized metal ion affinity chromatography（IMAC） using the interaction between a His-tag and nickel ions. Production of recombinant HDV antigen can reach 0.5 g/L under conditions of high-density cell fermentation. Applied to the diagnostic EliSA method, the recombinant HDV antigen shows excellent sensitivity（97% for IgM and 100% for IgG） and specificity（100% for IgG and IgM） for the detection of anti-HDV antibodies. Conclusion Expression and purification the recombinant HDV antigen as a candidate protein for application in a diagnostic EliSA for HDV infection. Large-scale production of the protein can be achieved using the high-density fermentation strategy.

关键词： EliSA diagnosis, HDV High-density cell fermentation IMAC

Centered Waves for the Two-dimensional Pseudo-Steady van der Waals Gas Satisfied Maxwell's Law Around a Sharp Corner

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Chinese Annals of Mathematics,Series B 2024年第04期45卷 537-554页

作者： Shuangrong li Wancheng sheng Department of Mathematics Shanghai University Shanghai 200444 China Department of Mathematics Zhejiang University of Science and Technology Department of Mathematics Newtouch Center for Mathematics of Shanghai University Shanghai University

In this paper, the authors study the centered waves for the two-dimensional(2D for short) pseudo-steady supersonic flow with van der Waals gas satisfied Maxwell's law around a sharp corner. In view of the initial value of the specific volume and the properties of van der Waals gas, the centered waves at the sharp corner are constructed by classification. It is shown that the supersonic incoming flow turns the sharp corner by a centered simple wave or a centered simple wave with right-contact discontinuity or a composite wave(jump-fan, fan-jump or fan-jump-fan), or a combination of waves and constant state. Moreover, the critical angle of the sharp corner corresponding to the appearance of the vacuum phenomenon is obtained.

关键词： Centered simple wave Composite wave Two-dimensional Euler equations Van der Waals gas

Incorporating contextual evidence to improve implicit discourse relation recognition in Chinese

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Frontiers of Computer Science 2024年第3期18卷 91-104页

作者： sheng XU Peifeng li Qiaoming ZHU School of Computer Science and Technology Soochow UniversitySuzhou 215000China

The discourse analysis task,which focuses on understanding the semantics of long text spans,has received increasing attention in recent *** a critical component of discourse analysis,discourse relation recognition aims to identify the rhetorical relations between adjacent discourse units(e.g.,clauses,sentences,and sentence groups),called arguments,in a *** works focused on capturing the semantic interactions between arguments to recognize their discourse relations,ignoring important textual information in the surrounding ***,in many cases,more than capturing semantic interactions from the texts of the two arguments are needed to identify their rhetorical relations,requiring mining more contextual *** this paper,we propose a method to convert the RST-style discourse trees in the training set into dependency-based trees and train a contextual evidence selector on these transformed *** this way,the selector can learn the ability to automatically pick critical textual information from the context(i.e.,as evidence)for arguments to assist in discriminating their *** we encode the arguments concatenated with corresponding evidence to obtain the enhanced argument ***,we combine original and enhanced argument representations to recognize their *** addition,we introduce auxiliary tasks to guide the training of the evidence selector to strengthen its selection *** experimental results on the Chinese CDTB dataset show that our method outperforms several state-of-the-art baselines in both micro and macro F1 scores.

关键词： discourse parsing discourse relation recognition contextual evidence selection