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Effect of IBD sera on expression of inducible and endothelial nitric oxide synthase in human umbilical vein endothelial cells

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World Journal of Gastroenterology 2006年第11期12卷 1730-1738页

作者： Károly Palatka Zoltán Serf(o|″)z(o|″) Zoltán Veréb Róbert Bátori Beáta Lontay Zoltán Hargitay Zoltán Nemes Miklós Udvardy Ferenc Erd(o|″)di István Altorjay Department of Gastroenterology University of Debrecen Nagyerdei k(o|¨)rút 98.Debrecen H-4001Hungary Department of Experimental Zoology BLRI-HAS TihanyHungary Institute of Immunology University of Debrecen Nagyerdei k(o|¨)rút 98.Debrecen H-4001 Hungary Department of Medical Chemistry University of Debrecen Nagyerdei k(o|¨)rút 98.Debrecen H-4001Hungary Department of Pathology Gyula Kenezy Hospital DebrecenBartók Béla út 10.Debrecen H-4026Hungary Institute of Pathology University of Debrecen Nagyerdei k(o|¨)rút 98.Debrecen H-4001Hungary Institute of Internal Medicine University of Debrecen Nagyerdei k(o|¨)rút 98.Debrecen H-4001Hungary

AIM： To study the expression of endothelial and inducible nitric oxide synthases （eNOS and iNOS） and their role in inflammatory bowel disease （IBD）. METHODS： We examined the effect of sera obtained from patients with active Crohn＇s disease （CD） and ulcerative colitis （UC） on the function and viability of human umbilical vein endothelial cells （HUVEC）. HUVECs were cultured for 0-48 h in the presence of a medium containing pooled serum of healthy controls, or serum from patients with active CD or UC. Expression of eNOS and iNOS was visualized by immunofluorescence, and quantified by the densitometry of Western blots. Proliferation activity was assessed by computerized image analyses of Ki-67 immunoreactive cells, and also tested in the presence of the NOS inhibitor, 10^-4 mol/L L-NAME. Apoptosis and necrosis was examined by the annexin-V-biotin method and by propidium iodide staining, respectively. RESULTS： In HUVEC immediately after exposure to UC, serum eNOS was markedly induced, reaching a peak at 12 h. In contrast, a decrease in eNOS was observed after incubation with CD sera and the eNOS level was minimal at 20 h compared to control （18%±16% vs 23%± 15% P〈0.01）. UC or CD serum caused a significant increase in iNOS compared to control （UC： 300%±21%; CD： 275% ± 27% vs 108% ± 14%, P〈0.01）. Apoptosis/necrosis characteristics did not differ significantly in either experiment. Increased proliferation activity was detected in the presence of CD serum or after treatment with L-NAME. Cultures showed tube-like formations after 24 h treatment with CD serum. CONCLUSION： IBD sera evoked changes in the ratio of eNOS/iNOS, whereas did not influence the viability of HUVEC. These involved down-regulation of eNOS and up-regulation of iNOS simultaneously, leading to increased proliferation activity and possibly a reduced antiinflammatory protection of endothelial cells.

关键词： Crohn's disease Human umbilical vein endothelial cells Inflammatory bowel disease Nitric oxide synthase Ulcerative colitis

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Lichen-Spruce Woodland Early Indicators of Ecological Resilience Following Silvicultural Disturbances in Québec’s Closed-Crown Forest Zone

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American Journal of Plant Sciences 2013年第3期4卷 749-765页

作者： Esteban Gonzalez Francois Hébert Jean-Francois Boucher Pascal Sirois Daniel Lord Département des Sciences Fondamentales Université du Québec à ChicoutimiSaguenayCanada Direction de la Recherche Forestière Ministère des Ressources NaturellesQuébecCanada

Lichen woodlands (LW) located in the closed-crown boreal forest are not a successional stage moving towards a closed black spruce feathermoss stand (FM), but an alternative stable state, due to their previous forest history, and the occurrence of LWs located nearby closed-crown FM stands. Therefore, afforestation in those LWs through site preparation and plantation could shift back LW into FM stands. We implemented an experimental design with different combinations of silvicultural treatments in both site types (LW, FM). We monitored the evolution of plant diversity and the physiology of three bio-indicators (Picea mariana, Kalmia angustifolia, Rhododendron groenlandicum) in different microsites created by the silvicultural treatments. The return to the initial composition was noticed only two years after treatments, especially in the LW stands, thus indicating a higher level of early ecosystem resilience in LWs compared to FM stands. Mean species cover, especially in the FM stands, decreased the most in the skid trails created by logging, probably due to a lack of acclimation of bryophytes to open stand conditions. Conversely, ericaceous shrubs and lichens found in the LWs were already acclimated to open stand conditions, which give to LWs a restructuring advantage compared to FM plant communities after silvicultural treatments. Overall, FM and LW short-term resilience was similar, indicating equally efficient ecosystem reorganization in both stands. The comparable early resilience in managed LW and FM stands, in terms of plant biodiversity, contradicts the presumed fragility of LW stands, especially in this case where LWs are assumed to be an alternative stable state created by compound disturbances. Silvicultural treatments maintained the functional group diversity in LWs, a key element for ecosystem resilience. Therefore, this study support the idea that plantation following site preparation in LWs could be a valuable management strategy to reach several objecti

关键词： Afforestation Black Spruce Kalmia angustifolia Rhododendron groenlandicum Logging Picea mariana Vegetation Recovery Scarification Silviculture

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2、3、7、8-四氯二苯二氧芑对人舌鳞癌细胞的乙氧基异吩(口恶)唑-0-去乙基酶的诱导作用

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上海医科大学学报 1991年第6期18卷 451-455页

作者：曹群立 Charles D.He'bert 上海医科大学预防医学研究所美国国立环境健康科学研究院

本文对多氯代芳香烃的高毒性化合物2、3、7、8-四氯二苯二氧芑(TCDD)诱导两株人舌鳞癌细胞(SCC-15G,SCC-25)的乙氧基异吩(口恶)唑-O-去乙基酶(EROD,P_(450)同工酶)的活性进行了观察,分别测定了剂量-效应曲线和时间反应曲线,提出了TCDD... 详细信息

本文对多氯代芳香烃的高毒性化合物2、3、7、8-四氯二苯二氧芑(TCDD)诱导两株人舌鳞癌细胞(SCC-15G,SCC-25)的乙氧基异吩(口恶)唑-O-去乙基酶(EROD,P_(450)同工酶)的活性进行了观察,分别测定了剂量-效应曲线和时间反应曲线,提出了TCDD对细胞的EROD的诱导作用要在细胞未达到融合之前反应敏感,两株细胞中对TCDD的诱导酶活性以SCC-15G更为敏感。提出TCDD对SCC细胞EROD的诱导、细胞增生和分化的影响趋势一致,推论P_(450)同工酶诱导可能为细胞恶性程度的一个标志。

关键词：舌鳞癌细胞 EROD TCDD 细胞株

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Enhancing sensitivity in atomic force microscopy for planar tip-on-chip probes

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Microsystems & Nanoengineering 2022年第3期8卷 39-49页

作者： H.Tung Ciftci Michael Verhage Tamar Cromwijk Laurent Pham Van bert Koopmans Kees Flipse Oleg Kurmosikov Department of Applied Physics Eindhoven University of TechnologyPO Box 5135600 MB Eindhoventhe Netherlands DRF/RAMIS/SPEC-LEPO Centre CEA de Saclay91191 Gif-sr-VvetteFrance Institut Jean Lamour Lorraine University54000 NancyFrance

We present a new approach to tuning-fork-based atomic force microscopy for utilizing advanced"tip-on-chip"probes with high sensitivity and broad ***,such chip-like probes with a size reaching 2×2 mm^(2)drastically perturb the oscillation of the tuning fork,resulting in poor performance in its intrinsic force ***,restoring initial oscillatory characteristics is necessary for regaining high *** this end,we developed a new approach consisting of three basic steps:tuning-fork rebalancing,revamping holder-sensor fixation,and electrode *** rebalancing allows the tuning fork to recover the frequency and regain high Q-factor values up to 10^(4)in air and up to 4×10^(4)in ultra-high vacuum *** floating-like holder-fxation using soft wires significantly reduces energy dissipation from the mounting *** with the soft wires,reconfigured electrodes provide electrical access to the chip-like probe without intervening in the force-sensing ***,our easy-to-implement approach allows converting the atomic force microscopy tip from a passive tool to a dedicated microdevice with extended functionality.

关键词： tuning atomic utilizing

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积极推广直邮信函

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印刷技术 2006年第33期 15-18页

作者： bert Berkley 全球信封联盟主席、美国兴德信封公司董事长

今天，我想讲以下四个议题：直邮信函的优势，全球信封制造业和印刷业的竞争和变化，美国兴德信封公司的发展历史，以及全球信封联盟（GEA）在全球范围内开展一场提高直邮信函的使用量及其价值的运动。

关键词：信函发展历史全球范围印刷业制造业信封使用量价值

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无纸仓库

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现代制造 2006年第27期 48-49页

作者： bert Bleicherlff

作为物流服务商或贸易公司,谁要想在市场上站稳脚跟,他就必须要对其物流流程进行无休止的优化。Hoffmann集团公司在其位于Nuernberg的物流中心的改造进程中,通过其语音控制的储运技术实现了准确的供货。

关键词：仓库物流中心贸易公司技术实现语音控制集团公司服务商市场

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不可超越的优势

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现代制造 2014年第12期 40-41页

作者： bert Brahmet

30多年来，液压驱动系统一直是冲压加工技术领域中的标准技术;时至今日，其在冲击技术领域中的优势仍然没有被伺服驱动技术的直接驱动方案所超越。而新的适于在中等厚度钣材加工中应用的驱动系统则代表了目前液压驱动技术的最高水平。

关键词：优势液压驱动系统伺服驱动技术液压驱动技术加工技术标准技术驱动方案冲击技术

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Impact of Low-Impact Development Technologies from an Ecological Perspective in Different Residential Zones of the City of Atlanta, Georgia

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Engineering 2018年第2期4卷 30-35页

作者： Zackery B. Morris Stephen M. Malone Abigail R. Cohen Marc J. Weissburg bert Bras George W. Woodruff School of Mechanical Engineering Georgia Institute of Technology Atlanta GA 30332-0405 USA School of Biological Sciences Georgia Institute of Technology Atlanta GA 30332 USA

Low-impact development （LID） technologies have a great potential to reduce water usage and stormwa- ter runoff and are therefore seen as sustainable improvements that can be made to traditional water infrastructure, These technologies include bioretention areas, rainwater capturing, and xeriscaping, all of which can be used in residential zones, Within the City of Atlanta, residential water usage accounts for 53% of the total water consumption; therefore, residential zones offer significant impact potential for the implementation of LID, This study analyzes the use of LID strategies within the different residen- tial zones of the City of Atlanta from an ecological perspective by drawing analogies to natural ecosys- tems, The analysis shows that these technologies, especially with the addition of a graywater system, work to improve the conventional residential water network based upon these ecological metrics, The higher metric values suggest greater parity with healthy, natural ecosystems.

关键词： Low-impact development Ecological network analysis Residential water consumption

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Surgical stress and cytoskeletal changes in lens epithelial cells following manual and femtosecond laser-assisted capsulotomy

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International Journal of Ophthalmology(English edition) 2020年第6期13卷 927-934页

作者： ANDrea Krisztina Sükosd Krisztina Szabadfi Edina Szabó-Meleg Beáta Gáspár Pavel Stodulka Gyorgy SétálóJr Róbert Gábriel Miklós Nyitrai Zsolt Biró Hajnalka Abrahám Department of Ophthalmology University of Pecs Medical School and Clinical CentrePecs 7623Hungary Department of Experimental Zoology and Neurobiology University of PecsPecs 7624Hungary Department of Biophysics University of Pecs Medical School and Clinical CentrePecs 7624Hungary Janos Szentagothai Research Centre University of PecsPecs 7624Hungary Optimum Laser Centre Budapest 1124Hungary Gemini Eye Clinics Zlin 76001Czech Republic Department of Medical Biology and Central Electron Microscopic Laboratory University of Pecs Medical SchoolPecs 7624Hungary

AIM:To study the effect of mechanical stress on the cytoskeleton in lens epithelial cells following conventional phacoemulsification surgery(CPS)and femtosecond laserassisted cataract surgery(FLACS).METHODS:The cytoskeleton of the epithelial cells of the anterior lens capsules(ALC)removed by CPS and FLACS was examined by *** of the intermediate filament,glial fibrillary acidic protein(GFAP),and glutamine synthetase(GS)immunoreactivity were *** order to map the actin network of cells,fluorescently labeled phalloidin was *** samples were examined using confocal laser scanning ***:GFAP expression was visible in a larger number of the epithelial cells after CPS compared to *** CPS sample’s epithelial cells,GFAP immunoreactivity indicated robust morphological *** the actin filaments,the presence of tubular elements connecting epithelial cells,regular actin pattern and marked cortical network after CPS were *** FLACS,the actin cytoskeleton of the epithelial cells remained densely structured,and the tubular elements were undetectable,however,the above-mentioned regular actin pattern and the marked cortical network were ***:The conventional removal of the ALC induces more robust changes of the cytoskeleton of the lens epithelial cells.

关键词： lens epithelial cell cytoskeleton capsulotomy glial fibrillary acidic protein actin

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全球生命伦理学

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医学与哲学（A） 2015年第1期36卷 17-23页

作者： Henk A.M.J.ten Have bert Gordijn 陈月芹迪尤肯大学都柏林城市大学山西医科大学公共卫生学院山西太原030001

探讨生命伦理学的起源,并阐述了不同理论支持者的观点,发现无论准确的生命伦理学历史起源是什么,目前生命伦理学已经变成了一种真正的全球现象。它在全世界具有重要的意义,因为人类不仅仅是欧洲人或亚洲人,也是世界公民和一个全球道德... 详细信息

探讨生命伦理学的起源,并阐述了不同理论支持者的观点,发现无论准确的生命伦理学历史起源是什么,目前生命伦理学已经变成了一种真正的全球现象。它在全世界具有重要的意义,因为人类不仅仅是欧洲人或亚洲人,也是世界公民和一个全球道德共同体的成员。生命伦理学对任何地方、任何人都是重要的,并不是因为它是"拿来"的,而是因为它提供了一个普遍的框架去解释和管理正在经历的变化,这个变化包括了目前所有的国家和文化。然而,这个框架的解释和应用必须依据本土环境。因此,现代生命伦理学具有全球性和本土特征的特点。

关键词：生命伦理学全球生命伦理学全球道德共同体

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