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Gene therapy with caspase-3 small interfering RNA-nanoparticles is neuroprotective after optic nerve damage

Neural Regeneration Research 2021年第12期16卷 2534-2541页

作者： Mohamed Tawfik Xiwei Zhang Lisa Grigartzik Peter Heiduschka Werner Hintz Petra Henrich-Noack Berend van Wachem bernhard A.Sabel Institute of Medical Psychology Otto von Guericke University of MagdeburgMagdeburgGermany Institute of Process Engineering Otto von Guericke University of MagdeburgMagdeburgGermany Department of Ophthalmology Münster University HospitalMünsterGermany Department of Neurology with Institute of Translational Neurology Münster University HospitalMünsterGermany Center of Behavioral Brain Sciences(CBBS) MagdeburgGermany

Apoptosis,a key mechanism of programmed cell death,is triggered by caspase-3 protein and lowering its levels with gene therapy may rescue cell death after central nervous system *** developed a novel,non-viral gene therapy to block caspase-3 gene expression using small interfering RNA(siRNA)delivered by polybutylcyanoacrylate nanoparticles(CaspNPs).In vitro CaspNPs significantly blocked caspase-3 protein expression in C6 cells,and when injected intraocularly in vivo,CaspNPs lowered retinal capsase-3 immunofluorescence by 57.9%in rats with optic nerve ***,repeated retinal ganglion cell counts using confocal neuroimaging showed that post-traumatic cell loss after intraocular CaspNPs injection was only 36.1%versus 63.4%in lesioned *** non-viral gene therapy with siRNA-nanoparticles can selectively silence caspace-3 gene expression and block apoptosis in post-mitotic neurons,siRNA delivery with nanoparticles may be promising for neuroprotection or restoration of central visual system damage and other neurological *** animal study procedures were approved by the German National Act on the use of experimental animals(Ethic Committee Referat Verbraucherschutz,Veterinärangelegenheiten;Landesverwaltungsamt Sachsen-Anhalt,Halle,Germany,#IMP/G/01-1150/12 and#IMP/G/01-1469/17).

关键词： apoptosis brain caspase-3 drug delivery gene therapy in vivo confocal neuroimaging nanoparticles neurodegeneration neuroprotection retina siRNA

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Color development in an extrusion-cooked model system

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International Journal of Agricultural and Biological Engineering 2008年第2期1卷 55-63页

作者： Hanwu Lei Roger Ruan R.Gary Fulcher bernhard van Lengerich Department of Agricultural and Biosystems Engineering South Dakota State UniversityBrookingsSD 57007USA The Department of Bioproducts and Biosystems Engineering University of MinnesotaSt.PaulMN 55108USA Department of Food Science and Nutrition University of MinnesotaSt.PaulMN 55108USA Department of Food Science University of ManitobaWinnipegMBR3T 2N2Canada General Mills Inc.9000 Plymouth Ave.N.Golden ValleyMN 55427USA

Rice-glucose-lysine blend was extruded using a co-rotating twin-screw *** effects of different extrusion conditions on the browning properties of extrudates were analyzed and compared using the CIE Lab system of *** process variables included moisture content,screw speed,barrel temperature,and screw *** influence of product temperature on the browning property of extrudates was significant(P<0.05).The color parameters were related to product temperature by a 4th degree polynomial(P<0.05).Hunter color scale values(L*,a*,b*,-L*a*/b*,Whiteness Index,and Yellowness Index)from extruded samples were analyzed to relate to extrusion process *** temperature and browning properties were modeled and tested at various screw configurations and extrusion *** temperature and browning property models were verified using different screw geometries and other processing conditions with reasonable accuracy.

关键词： twin-screw extrusion browning hunter color scale Maillard reaction empirical modeling

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Molecular Mechanism of Electroacupuncture Regulating Cerebral Arterial Contractile Protein in Rats with Cerebral Infarction Based on MLCK Pathway

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Chinese Journal of Integrative Medicine 2023年第1期29卷 61-68页

作者： LI Jing ZHANG Min HE Ying DU Yuan-hao ZHANG Xue-zhu Rainer Georgi bernhard Kolberg Xu Yan-long Institute of Acupuncture and Moxibustion First Teaching Hospital of Tianjin University of Traditional Chinese MedicineNational Clinical Research Center for Chinese Medicine Acupuncture and MoxibustionTianjin300193China Max Planck Institute for Medical Research Heidelberg69120Germany Uniklinik Mannheim Mannheim68167Germany Department of Acupuncture and Moxibustion Gansu Provincial Hospital of Traditional Chinese MedicineLanzhou730050China

Objective To explore the effect of electroacupuncture(EA)intervention on the vasoconstriction of cerebral artery smooth muscle cells after cerebral *** Male Wistar rats were randomly divided into 3 groups by a random number table:the model group(n=24),the EA group(n=24),and the normal group(n=6).The model and the EA groups were divided into different time subgroups at 0.5,1,3,and 6 h after middle cerebral artery occlusion(MCAO),with 6 rats in each *** model was established using intraluminal suture occlusion *** EA group was given EA treatment at acupoint Shuigou(GV 26)instantly after MCAO for 20 *** contents of cerebrovascular smooth muscle MLCK,the 3 subunits of myosin light chain phosphatase(MLCP)MYPT1,PP1c-δand M20,as well as myosin-ATPase activity were detected using immunohistochemistry and Western *** The overall expression level of the MYPT1 and PP1c-δin the model group was significantly higher(P0.05),and the other subgroups were still significantly higher than the normal group(P<0.01).After EA intervention,the expression level of each subgroup was significantly lower than the corresponding model *** was a significant difference between the 0.5 and 1 h subgroups(P<0.01),while a difference was also observed between the 3 and 6 h subgroups(P<0.05).The dynamic change rule gradually increased with the prolongation of infarction time within 6 h after *** EA intervention can inhibit contraction of cerebral vascular smooth muscle cells and regulate smooth muscle relaxation by regulating MLCK pathway.

关键词： infarction vasomotor MLCK pathway Shuigou(GV 26) electroacupuncture

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What Do You Know about China Anyway?

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China & The World Cultural Exchange 2000年第4期66卷 40-41页

作者： bernhard Bartsch

OnthenightoftheMaca0handover,IwassittinginalittlebarwithtwofriendsfromDenmarkandAustralia,***,aboutallthepeoplehavingtostandout-sideintheDecembercold,justbecauseafewcasinosandbrothelsarebackun-derChinesecontro（共2页)

关键词： What Do You Know about China Anyway

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Method and Apparatus for Creating Problem-Solving Complexes from Individual Elements

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Advances in Bioscience and Biotechnology 2014年第4期5卷 311-315页

作者： bernhard Mitterauer Volitronics-Institute for Basic Research Psychopathol-ogy and Brain Philosophy University of Salzburg Salz-burg Austria

Based on the biological key-lock-principle common in various biological systems such as the human brain, this paper relates to a method and device for creating problem-solving complexes from individual elements that can be coupled with one another and that have different properties to solve problems. The problem solution can be carried out either serially with a large computer, or with several independent, hierarchically joined computers. In this system, an independent control unit that assumes a multitude of tasks and also acts as an interface with access to all participating computers, is assigned to each problem or object class according to the amount of potential problem-oriented solutions. Such a unit prepares the partial solutions found in its computer for the totality of the solutions computed in the associated computers, finally leading to a total problem solution.

关键词： Key-Lock-Principle Geometrical Elements Computer System Composition Problem-Solving

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Warnung

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德语学习 2007年第2期 6-7页

作者： Thomas bernhard

Ein Kaufmann aus Koblenz hatte sich im vergangenen Jahr seinen Lebenswunsch erfüllt, die Pyramiden von Gizeh 1 zu besuchen und hatte, nachdem er die Pyramiden besucht gehabt hatte, diesen Besuch als die grte Enttuschung seines Lebens bezeichnen müssen, was ich verstehe, denn ich selbst war im vergangenen Jahr in gypten und vor allem von den Pyramiden enttuscht gewesen. Whrend ich selbst jedoch meine Enttuschung sehr rasch überwunden hatte, rchte der Koblenzer Kaufmann seine Enttuschung dadurch, dass er monatelang seitenlange Inserate2 in allen wichtigen Zeitungen Deutschlands, der Schweiz und sterreichs verffentlichte, in welchen er alle zukünftigen Besucher gyptens vor den Pyramiden, vor allem aber vor der berühmten Cheopspyramide, die ihn mehr noch als die andern zutiefst enttuscht hatte, warnte. Der Koblenzer Kaufmann hat mit diesen von ihm selbst sogenannten Antigypten und Antipyramidenplakaten in der kürzesten Zeit sein Vermgen aufgebraucht und sich ins totale Unglück gestürzt. Seine Inserate hatten auf die gyptenreisenden naturgemnicht den von ihm erhofften Einflu gehabt, im Gegenteil, vergrerte sich die Zahl derer, die heuer gypten besuchten, gegenüber jenen des Vorjahres um das Doppelte.

关键词： der Warnung

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A letter of congratulations for the launching of Global Health Journal from WHO Representative in China

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Global Health Journal 2017年第2期1卷 1-2页

作者： bernhard Schwartlaender WHO Representative in China

Dear President Hao Yang,My heartfelt congratulations to you and your colleagues at the People's Medical Publishing House for successfully launching of the Global Health Journal this week in Xi'*** launching of the jou...

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Burden of respiratory syncytial virus infection in young children

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World Journal of Clinical Pediatrics 2012年第3期1卷 8-12页

作者： bernhard Resch Research Unit for Neonatal Infectious Diseases and Epidemiology Division of Neonatology Department of Paediatrics Medical University of Graz

Respiratory syncytial virus(RSV) is the most frequent and important cause of lower respiratory tract infection in infants and children. It is a seasonal virus, with peak rates of infection occurring annually in the cold season in temperate climates, and in the rainy season, as temperatures fall, in tropical climates. High risk groups for severe RSV disease include infants below six mo of age, premature infants with or without chronic lung disease, infants with hemodynamically significant congenital heart disease, infants with immunodeficiency or cystic fibrosis, and infants with neuromuscular diseases. Mortality rates associated with RSV infection are generally low in previous healthy infants(below 1%), but increase significantly in children with underlying chronic conditions and comorbidities. Following early RSV lower respiratory tract infection, some patients experience recurrent episodes of wheezing mimicking early childhood asthma with persistence of lung function abnormalities until adolescence. There is currently no RSV vaccine available, but promising candidate vaccines are in development. Palivizumab, a monoclonal RSV antibody that is the only tool for immunoprophylaxis in high-riskinfants, lowers the burden of RSV infection in certain carefully selected patient groups.

关键词： Children Epidemiology Infant Palivizumab Respiratory syncytial virus Respiratory tract infection Risk factors Vaccine

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Novel Treatment Approach in Schizophrenia: Substitution of Glial Binding Proteins

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Advances in Bioscience and Biotechnology 2016年第10期7卷 392-402页

作者： bernhard J. Mitterauer Volitronics-Institute for Basic Research Wals Austria

In chronic schizophrenia, synaptic information processing is unbalanced, as shown in a model of glial-neuronal synaptic units, called tripartite synapses. The glial component of the synapse exerts a modifying function in neurotransmission since the astrocyte activated by neurotransmitters produces gliotransmitters that negatively feedback to the presynapse. It is hypothesized that in schizophrenia nonfunctional astrocytic receptors cannot be activated, thus losing their modulating function. This causes a generalization of information processing in the neuronal networks such that the brain is unable to distinguish between subjects and objects in the environment. Delusions, hallucinations and cognitive impairment occur on the behavioral level. In a model of a cholinergic tripartite synapse, it is shown that glial binding proteins modify neurotransmission by occupancy with cognate neurotransmitters temporarily turning off neurotransmission on the presynapse. Most recently, glial binding proteins have been engineered. It is proposed that the substitution of glial binding proteins may balance synaptic information processing in schizophrenia since these proteins exert a modulatory function comparable to functional astrocytic receptors. Rap- id technical developments may enable this novel treatment approach in schizophrenia.

关键词： Schizophrenia Synaptic unbalance Astrocytic Receptors Glial Binding Protein Treatment

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Effect of clear-cutting silviculture on soil respiration in a subtropical forest of China

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Journal of Plant Ecology 2013年第5期6卷 335-348页

作者： Yinlei Ma Yan Geng Yuanyuan Huang Yue Shi Pascal A.Niklaus bernhard Schmid Jin-Sheng He Department of Ecology College of Urban and Environmental Sciencesand Key Laboratory for Earth Surface Processes of the Ministry of EducationPeking UniversityBeijing 100871China Institute of Evolutionary Biology and Environmental Studies University of ZurichWinterthurerstrasse 190CH-8057 ZurichSwitzerland Key Laboratory of Adaptation and Evolution of Plateau Biota Northwest Institute of Plateau BiologyChinese Academy of SciencesXining 810008China

Aims Clear-cutting is a common forest management practice,especially in subtropical ***,the potential ecological consequences of clear-cutting remain *** particular,the effect of clear-cutting on soil processes,such as the carbon cycle,has not been quantified in subtropical ***,we investigated the response of soil respiration(Rs)to clear-cutting during a 12-month period in a subtropical forest in eastern *** We randomly selected four clear-cut(CC)plots and four corresponding undisturbed forest(UF)*** of Rs were made at monthly time points and were combined with continuous climatic measurements in both CC and *** Rs was estimated by interpolating data with an exponential model dependent on soil *** Rs was cumulated to annual Rs *** Findings In the first year after clear-cutting,annual estimates of Rs in CC(508±23g C m^(−2) yr^(−1))showed no significant difference to UF plots(480±12g C m^(−2) yr^(−1)).During the summer,soil temperatures were usually higher,whereas the soil volumetric water content was lower in CC than in UF *** long-term effects of clear-cutting on Rs are not significant,although there might be effects during the first several months after *** with previous work,this pattern was more pronounced in our subtropical forest than in the temperate and boreal forests that have been studied by *** aboveground residuals off-site after clear-cutting,our results indicate that the stimulation of increasing root debris,as well as environmental changes,will not lead to a significant increase in *** addition,long-term Rs will not show a significant decrease from the termination of root respiration,and this observation might be because of the influence of fast-growing vegetation after clear-cutting in situ.

关键词： clear-cutting subtropical forest soil respiration soil temperature

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