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Differentiation potential of human adipose tissue derived stem cells into photoreceptors through explants culture and enzyme methods

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International Journal of Ophthalmology(English edition) 2017年第1期10卷 23-29页

作者： Wei-Wei Xu li Huang Kelvin K.L.Chong Doreen S.Y.Leung benjamin el.li Zheng-Qin Yin Yi-Fei Huang Chi Pui Pang Department of Ophthalmology and Visual Sciences theChinese University of Hong KongHong Kong 999077China Department of Ophthalmology Chinese People's LiberationArmy General HospitalBeijing 100853China

AIM： To investigate the retinal photoreceptor differentiation potential of human orbital adipose tissue-derived stem cells （ADSCs） generated by enzyme （EN） and explant （EX） culture ***： We investigated potentials of human orbital ADSCs to differentiate into photoreceptors through EN and EX culture methods. EN and EX orbital ADSCs were obtained from the same donor during rehabilitative orbital decompression, and then were subject to a 3-step induction using Noggin, DKK-1, IGF-1 and b-FGF at different time points for 38d. Stem cell, eye-field and photoreceptor-related gene and protein markers were measured by reverse transcription-polymerase chain reaction （RT-PCR） and immunofluorescent （IMF） ***： Both EX and EN orbital ADSCs expressed CD133, a marker of cell differentiation. Moreover, PAX6 and rhodopsin, markers of the retinal progenitor cells, were detected from EX and EN orbital ADSCs. In EX orbital ADSCs, PAX6 mRNA was detected on the 17th day and then the rhodopsin mRNA was detected on the 24th day. In contrast, the EN orbital ADSCs expressed PAX6 and rhodopsin mRNA on the 31st day. EX orbital ADSCs expressed rhodopsin protein on the 24th day, while EN orbital ADSCs expressed rhodopsin protein on the 31st day. CONCLUSION： Orbital ADSCs isolated by direct explants culture show earlier and stronger expressions of markers towards eye field and retinal photoreceptor differentiation than those generated by conventional EN method.

关键词： photoreceptor cells cell differentiation adultstem cells tissue engineering explants culture enzymaticdigestion

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Seasonal compensation implied no weakening of the land carbon sink in the Northern Hemisphere under the 2015/2016 el Niño

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Science China Earth Sciences 2024年第1期67卷 281-294页

作者： Fangzhong SHI Xiuchen WU Xiaoyan li Philippe CIAIS Hongyan liU Chao YUE Yuting YANG Shulei ZHANG Shushi PENG Yi YIN benjamin POULTER Deliang CHEN State Key Laboratory of Earth Surface Processes and Resource Ecology Beijing Normal UniversityBeijing 100875China Faculty of Geographical Science Beijing Normal UniversityBeijing 100875China Key Laboratory of Tibetan Plateau Land Surface Processes and Ecological Conservation Qinghai Normal UniversityXining 810016China Research and Development Center for Watershed Environmental Eco-Engineering Beijing Normal University(Zhuhai)Zhuhai 519085China Laboratoire des Sciences du Climat et de l’Environnement(LSCE) CEA-CNRS-UVSQUMR821291191Gif-sur-YvetteFrance College of Urban and Environmental Science MOE Laboratory for Earth Surface ProcessesPeking UniversityBeijing 100871China State Key Laboratory of Hydroscience and Engineering Department of Hydraulic EngineeringTsinghua UniversityBeijing 100084China Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai) School of Atmospheric SciencesSun Yat-sen UniversityGuangzhou 510275China California Institute of Technology PasadenaCA 91125USA Biospheric Sciences Laboratory NASA Goddard Space Flight CenterGreenbeltMaryland 20771USA Regional Climate Group Department of Earth SciencesUniversity of GothenburgGothenburg SE-40530Sweden

The recurrent extreme el Niño events are commonly linked to reduced vegetation growth and the land carbon sink over many but discrete regions of the Northern Hemisphere(NH).However,we reported here a pervasive and continuous vegetation greening and no weakened land carbon sink in the maturation phase of the 2015/2016 el Niño event over the NH(mainly in the extra-tropics),based on multiple evidences from remote sensing observations,global ecosystem model simulations and atmospheric CO_(2)*** discovered a significant compensation effect of the enhanced vegetation growth in spring on subsequent summer/autumn vegetation growth that sustained vegetation greening and led to a slight increase in the land carbon sink over the spring and summer of 2015(average increases of 23.34%and 0.63%in net ecosystem exchange from two independent datasets relative to a 5-years average before the el Niño event,respectively)and spring of 2016(6.82%),especially in the extra-tropics of the NH,where the water supply during the pre-growing-season(November of the previous year to March of the current year)had a positive *** seasonal compensation effect was much stronger than that in 1997 and 1998 and significantly alleviated the adverse impacts of the 2015/2016 el Niño event on vegetation growth during its maturation *** legacy effect of water supply during the pre-growing-season on subsequent vegetation growth lasted up to approximately six *** findings highlight the role of seasonal compensation effects on mediating the land carbon sink in response to episodic extreme el Niño events.

关键词： el Niño Seasonal compensation effect Vegetation greening Land carbon sink Net ecosystem exchange Legacy effect

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Mirrorless MEMS imaging:a nonlinear vibrational approach utilizing aerosol-jetted PZT-actuated fiber MEMS scanner for microscale illumination

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Microsystems & Nanoengineering 2024年第1期10卷 175-192页

作者： Wei-Chih Wang Ming-Yao li Kuan-Chang Peng Yi-Feng Hsu benjamin Estroff Pao-Yun Yen David Schipf Wen-Jong Wu Department of Power Mechanical Engineering National Tsing Hua UniversityNo.101Section 2Kuang-Fu RoadHsinchuTaiwan 30013China Institute of NanoEngineering and Microsystems National Tsing Hua UniversityNo.101Section 2Kuang-Fu RoadHsinchuTaiwan 30013China Department of Mechanical Engineering University of WashingtonSeattleWA 98195USA Department of electrical Engineering University of WashingtonSeattleWA 98195USA Department of Engineering Science and Ocean Engineering National Taiwan UniversityTaiwanChina

This study introduces a novel image capture and lighting techniques using a cutting-edge hybrid MEMS scanner system designed for compact microscopic *** scanner comprises a tapered optical fiber waveguide and innovative aerosol-jet printed PZT(lead zirconate titanate)bimorph push-pull actuators on a stainless-steel substrate,effectively addressing issues that are commonly associated with PZT on silicon substrates such as fracture and layer *** leveraging nonlinear vibration,the scanner achieves a spiral scan pattern from a single signal input,in addition to the expected two-dimensional scanning and target illumination from two phase-shifted *** capability is further enhanced by a novel process to taper the optical fiber,which reduces illumination scattering and tunes the fiber to the resonant frequencies of the *** precisely tapered tip enables large fields of view while maintaining independent 2-axis scanning through one-degree-of-freedom *** validation showcases the successful generation of a spiral scan pattern with a 60μm diameter scan area and a 10 Hz frame rate,effectively reconstructing scanned images of 5μm lines,cross patterns(15μm in length with a 5μm gap),and structures of a Psychodidae wing.

关键词： steel fiber scanner

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Assessing the Influence of Phosphorus Fertilization on the Growth and Yield of Maize/Soybean Intercrop by Analyzing Nitrogen Uptake

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Natural Resources 2024年第8期15卷 189-210页

作者： Bertha Magombo Chunjie li benjamin Kolie Department of Resource and Environmental Science and Plant Protection China Agricultural University Beijing China Institute of Geographic Sciences and Natural Resources Research Chinese Academic of Science Beijing China Department of Resources and Environmental Management University of the Chinese Academy of Sciences Beijing China

Intercropping, particularly the combination of maize and soybeans, has been widely recognized for its potential to improve nitrogen uptake and promote sustainable agriculture. This study examines the patterns of nitrogen uptake in maize and soybean intercropping systems under different growth stages and phosphorus fertilization levels and investigates the influence of nitrogen uptake on growth parameters such as plant height, leaf area, and biomass accumulation in the maize/soybean intercrop under different phosphorus fertilization regimes. The study also collected chlorophyll samples at different growth stages of maize in monoculture and intercropping with maize or soybean. The results showed that plant height was greater in V10 in both fertilized and unfertilized treatments for intercropped maize and soybean, and chlorophyll concentration was higher in VT intercropped maize. The results also showed a higher accumulation of biomass. Understanding the growth dynamics of these plants in monoculture and intercropping systems and the impact of fertilization practices is crucial for optimizing crop productivity and sustainability in agricultural systems.

关键词： Intercropping Fertilization Chlorophyll Maize/Soybeans Nitrogen

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Assessment of Nitrogen Fixation, Uptake, and Leaching in Maize/Soybean Intercropping System at Varied Soil Depths and under Phosphorus Application in Chinese Agricultural Settings

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Natural Resources 2024年第7期15卷 173-187页

作者： Bertha Magombo Chunjie li benjamin Kolie China Agricultural University Department of Resource and Environmental Science and Plant Protection Beijing China Institute of Geographic Sciences and Natural Resources Research Chinese Academic of Science Beijing China Department of Resources and Environmental Management University of the Chinese Academy of Sciences Beijing China

The study of Nitrogen fixation, uptake, and leaching at different soil depths in the co-cultivation of maize and soybean under phosphorus fertilization is important for sustainable agriculture. This study was conducted in Quzhou, Hebei Province, China, with MC812 maize and Jidou12 soybean varieties. Soil samples were taken from each plot to create a composite sample. The results show that nitrogen concentration varies at different depths and is higher in all treatments between 40 and 100 cm. Incorporating intercropping of maize and soybeans into farming practices can lead to more sustainable and environmentally friendly agriculture in China.

关键词： Nitrogen Maize/Soybean Fertilization Intercropping Soil Fixation

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Towards seamless environmental prediction-development of Pan-Eurasian EXperiment(PEEX)modelling platform

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Big Earth Data 2024年第2期8卷 189-230页

作者： Alexander Mahura Alexander Baklanov Risto Makkonen Michael Boy Tuukka Petäjä Hanna KLappalainen Roman Nuterman Veli-Matti Kerminen Stephen R.Arnold Markus Jochum Anatoly Shvidenko Igor Esau Mikhail Sofiev Andreas Stohl Tuula Aalto Jianhui Bai Chuchu Chen Yafang Cheng Oxana Drofa Mei Huang Leena Järvi Harri Kokkola Rostislav Kouznetsov Tingting li Piero Malguzzi Sarah Monks Mads Bruun Poulsen Steffen M.Noe Yuliia Palamarchuk benjamin Foreback Petri Clusiu Till Andreas Soya Rasmussen Jun She Jens Havskov Sørensen Dominick Spracklen Hang Su Juha Tonttila Siwen Wang Jiandong Wang Tobias Wolf-Grosse Yongqiang Yu Qing Zhang Wei Zhang Wen Zhang Xunhua Zheng Siqi li Yong li Putian Zhou Markku Kulmala Faculty of Science Institute for Atmospheric and Earth System Research(INAR)/PhysicsUniversity of Helsinki(UHEL)HelsinkiFinland Science and Innovation Department World Meteorological Organization(WMO)GenevaSwitzerland Niels Bohr Institute University of CopenhagenCopenhagenDenmark Climate System Research Finnish Meteorological Institute(CSR-FMI)HelsinkiFinland School of Engineering Sciences Lappeenranta University of TechnologyLappeenrantaFinland Institute for Climate and Atmospheric Science School of Earth and EnvironmentUniversity of LeedsLeedsUK Biodiversity and Natural Resources Program International Institute for Applied Systems Analysis(IIASA)LaxenburgAustria Department of Physics and Technology UiT-The Arctic University of NorwayTromsoNorway Nansen Environmental and Remote Sensing Center(NERSC) BergenNorway Atmospheric Composition Finnish Meteorological Institute(AC-FMI)HelsinkiFinland Norwegian Institute for Air Research(NILU) KjellerNorway LAGEO Institute of Atmospheric PhysicsChinese Academy of Sciences(IAP-CAS)BeijingChina Max Planck Institute for Chemistry(MPI-Chem) MainzGermany Institute of Atmospheric Sciences and Climate National Research Council of Italy(CNR-15SAC)BolognaItaly Institute of Geographic Sciences and Natural Resources Research(IGSNRR) Chinese Academy of Sciences(CAS)BeijingChina Faculty of Science Helsinki Institute of Sustainability Science(HELSUS)University of Helsinki(UHEL)HelsinkiFinland Atmospheric Research Centre of Eastern Finland Finnish Meteorological Institute(ARCEF-FMI)KuopioFinland Institute of Atmospheric Physics Chinese Academy of Sciences(IAP-CAS)BeijingChina Department of Plant Physiology Estonian University of Life Sciences(EULS)Institute of Agricultural and Environmental Sciences(IAES)TartuEstonia Danish Meteorological Institute(DMI) CopenhagenDenmark State Key Laboratory of Atmospheric Boundary Layer Physics and Atmospheric Chemistry LAPC) Institute of Atmospheric Physics(IAP)Chinese Academy of Sciences(CAS)BeijingChina Uni

The Pan-Eurasian Experiment Modelling Platform(PEEX-MP)is one of the key blocks of the PEEX Research *** PEEX MP has more than 30 models and is directed towards seamless envir-onmental *** main focus area is the Arctic-boreal regions and *** models used in PEEX-MP cover several main components of the Earth’s system,such as the atmosphere,hydrosphere,pedosphere and biosphere,and resolve the physicalchemicalbiological processes at different spatial and temporal scales and *** paper introduces and discusses PEEX MP multi-scale modelling concept for the Earth system,online integrated,forward/inverse,and socioeconomical modelling,and other approaches with a particular focus on applications in the PEEX geographical *** employed high-performance com-puting facilities,capabilities,and PEEX dataflow for modelling results are *** virtual research platforms(PEEXView,Virtual Research Environment,Web-based Atlas)for handling PEEX modelling and observational results are *** over-all approach allows us to understand better physical-chemicalbiological processes,Earth’s system interactions and feedbacks and to provide valuable information for assessment studies on evaluating risks,impact,consequences,*** population,envir-onment and climate in the PEEX *** work was also one of the last projects of *** Zilitinkevich,who passed away on 15 February *** the finalization took time,the paper was actually submitted in 2023 and we could not argue that the final paper text was agreed with him.

关键词： Multi-scale and-processes modelling concept seamless coupling high-performance computing data infrastructure virtual research platforms

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A new rat model of portal hypertension induced by intraportal injection of microspheres

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World Journal of Gastroenterology 1998年第1期4卷 71-74页

作者： li Xiang-Nong IS benjamin B Alexander Department of liver Surgery Affiliated Hospital Xuzhou Medical College Xuzhou 221002 China Department of liver Surgery Affiliated Hospital Xuzhou Medical College China Xuzhou Medical College China Beijing Medical University China Department of Surgery King's College School and Dentistry United Kingdom Department of liver Surgery Affiliated Hospital Xuzhou Medical College 50 Jiaheqian Street Xuzhou 221002 China Department of Surgery King's Coll. Sch. of Med. and Dent. London United Kingdom

IM To produce a new rat model of portal hypertension by intraportal injection of microspheres.METHODS Measured aliquots of single or differentsized microspheres (15, 40, 80μm) were injected into the portal vein to block intrahepatic portal radicals. The resultant changes in arterial, portal, hepatic venous and splenic pulp pressures were monitored. The liver and lungs were excised for histological examination.RESULTS Portal venous pressure was elevated from basal value of 089-102 kPa to a steadystate of 198-319 kPa following the sequential injections of single or differentsized microspheres, with a markedly lowered mean arterial pressure. However, a smalldose injection of 80μm microspheres (18×105) produced a steadystate portal venous pressure of 253±017 kPa, and all rats showed normal arterial pressures. In addition, numerous microspheres were found in the lungs in all experimental groups.CONCLUSION Portal hypertension can be reproduced in rats by intraportal injection of microspheres at a small dose of 80μm (18×105). Intrahepatic portalsystemic shunts probably exist in the normal rat liver..

关键词： portal vein hypertension,portal disease model,animal latex microsphere

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Double Peak Derived from Piezoelectric Coefficient Nonlinearity and Proposal for Self-Powered Systems

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Transactions of Nanjing University of Aeronautics and Astronautics 2018年第1期35卷 109-115页

作者： Zhang Bin li Dezhi li Yingrui Ducharne benjamin Gao Jun School of Mechanical Electrical & InformationShandong University School of Mechatronics Engineering Harbin Institute of Technology Laboratoire de Génie electrique et Ferroélectricité(LGEF) INSA de Lyon

With the development of wireless sensor network(WSN)applications in intelligent monitoring,additional support for the low power consumption wireless nodes can be provided by piezoceramics that harvest vibrational ***,we describe the effects of stimulation variations on piezoceramics and the energy harvesting circuit *** types of piezoceramics were stimulated at different frequencies and amplitudes to obtain the power output ***,the energy harvesting circuit was studied and coupled with the piezoceramics.A double peak phenomenon was found in energy harvesting using a hard piezoceramic which gave a direct proof that the nonlinearity of the piezo constant should be considered in ***,energy storage and output were studied and *** components for the WSN were recommended according to the output power and the *** results will give an instruction for piezoceramic energy harvesting under various stress amplitudes on its implementation.

关键词： piezoelectric energy harvesting self-powered system energy harvesting circuit output optimization

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Multimodal Adaptive Identity-Recognition Algorithm Fused with Gait Perception

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Big Data Mining and Analytics 2021年第4期4卷 223-232页

作者： Changjie Wang Zhihua li benjamin Sarpong Department of Computer Science and Technology School of Artificial Intelligence and Computer ScienceJiangnan UniversityWuxi 214122China

Identity-recognition technologies require assistive equipment,whereas they are poor in recognition accuracy and *** overcome this deficiency,this paper proposes several gait feature identification ***,in combination with the collected gait information of individuals from triaxial accelerometers on smartphones,the collected information is preprocessed,and multimodal fusion is used with the existing standard datasets to yield a multimodal synthetic dataset;then,with the multimodal characteristics of the collected biological gait information,a Convolutional Neural Network based Gait Recognition(CNN-GR)model and the related scheme for the multimodal features are developed;at last,regarding the proposed CNN-GR model and scheme,a unimodal gait feature identity single-gait feature identification algorithm and a multimodal gait feature fusion identity multimodal gait information algorithm are *** results show that the proposed algorithms perform well in recognition accuracy,the confusion matrix,and the kappa statistic,and they have better recognition scores and robustness than the compared algorithms;thus,the proposed algorithm has prominent promise in practice.

关键词： gait recognition person identification deep learning multimodal feature fusion

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Infant Cry Language Analysis and Recognition:An Experimental Approach

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IEEE/CAA Journal of Automatica Sinica 2019年第3期6卷 778-788页

作者： lichuan liu Wei li Xianwen Wu benjamin X.Zhou IEEE the Department of electrical Engineering Northern Illinois University the Department of Biology The College of New Jersey

Recently, lots of research has been directed towards natural language processing. However, the baby's cry, which serves as the primary means of communication for infants, has not yet been extensively explored, because it is not a language that can be easily understood. Since cry signals carry information about a babies' wellbeing and can be understood by experienced parents and experts to an extent, recognition and analysis of an infant's cry is not only possible, but also has profound medical and societal applications. In this paper, we obtain and analyze audio features of infant cry signals in time and frequency *** on the related features, we can classify given cry signals to specific cry meanings for cry language recognition. Features extracted from audio feature space include linear predictive coding(LPC), linear predictive cepstral coefficients(LPCC),Bark frequency cepstral coefficients(BFCC), and Mel frequency cepstral coefficients(MFCC). Compressed sensing technique was used for classification and practical data were used to design and verify the proposed approaches. Experiments show that the proposed infant cry recognition approaches offer accurate and promising results.

关键词： Compressed sensing feature extraction infant cry signal language recognition

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