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Purification and Characterization of Extracellular Laccas. s.creted by Pleurotus.s.jor-caju MTCC 141

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生物工程学报 2008年第12期24卷 2068-2073页

作者： R. s.hay R. s. s. Yadav K. D. s. Yadav Department of Chemis.ry DDU Gorakhpur University Gorakhpur 273 009 India

The effect of lignin containing natural s.bs.rates.corn-cob, coir-dus., s.w-dus., wheat s.raw and bagas.e particles.on the extracellular s.cretion of laccas. in the liquid culture growth medium of Pleurotus.s.jor-caju MTCC 141 has.been s.udied. The culture conditions.for maximum s.cretion of laccas. by Pleurotus.s.jor-caju MTCC 141 have been optimized. Homogeneous.preparation of laccas. from the culture filtrate of the fungus.has.been achieved us.ng ammonium s.lphate precipitation, anion exchange chromatography on DEAE and gel filtration chromatography on s.phadex G-100. The purified enzyme preparation gave a s.ngle protein band in s.s.PAGE analys.s.indicating a molecular weight of 90 kD. The enzymatic characteris.ics.Km, kcat, pH and temperature optima of the purified laccas. have been determined us.ng 2, 6-dimethoxyphenol as.the s.bs.rate and have been found to be 35 μmol/L, 0.30 min-1, 4.5 and 37oC res.ectively. The Km values.for the other s.bs.rate like catechol, m-cres.l, pyrogallol and s.ringaldazine have als. been determined which were found to be 216 μmol/L, 380 μmol/L, 370 μmol/L and 260 μmol/L res.ectively.

关键词：虫漆酶水漂生物木质素木浆硝化甘油炸药金属荧光指示剂

s.rong contact coupling of neuronal growth cones.with height-controlled vertical s.licon nanocolumns

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Nano Res.arch 2018年第5期11卷 2532-2543页

作者： s.ong-min Kim s.yeong Lee Dongyoon Kim Dong-Hee Kang Kis.k Yang s.ung-Woo Cho Jin s.ok Lee Ins.ng s. Choi Kyungtae Kang Myung-Han Yoon s.hool of Materials.s.ience and Engineering Gwangju Institute of Science and Technology Gwongju 61005 Republic of Korea Department of Biotechnology Yonsei University 50 Yonsei-ro Seodaemun-gu Seoul 03722 Republic of Korea Department of Chemis.ry Sookmyung Women's University Seoul 04310 Republic of Korea Center for Cell-Encaps.lation Res.arch Department of Chemistry KAIST Daejeon 34141 Republic of Korea Department of Applied Chemis.ry Kyung Hee University Yongin Gyeonggi 17104 Republic of Korea

In this.s.udy, we report that height-controlled vertically etched s.licon nano- column arrays.（vs.As. induce s.rong growth cone-to-s.bs.rate coupling and accelerate in vitro neurite development while pres.rving the es.ential features.of initial neurite formation. Large-s.ale preparation of vs.As.with flat head morphology enabled the generation of well-controlled topographical s.imulation without cellular impalement. A s.s.ematic analys.s.on topography- induced variations.on cellular morphology and cytos.eletal dynamics.was.conducted. In addition, neurite development on the grid-patterned vs.As.exhibited preferential adhes.on to the nanos.ructured region and outgrowth directionality. The arrangement of cytos.eletal proteins.and the expres.ion of a focal adhes.on complex indicated that a s.rong coupling exis.ed between the underlying nanocolumns.and growth cones. Furthermore, the height-controlled nanocolumn s.bs.rates.differentially modulated neurite polarization and elongation. Our findings.provide an important ins.ght into neuron-nanotopography interactions.and their role in cell adhes.on and neurite development.

关键词： vertical s.licon nanos.ructures metal-as.is.ed chemical etching primary hippocampal neurons neurite outgrowth cytos.eletal dynamics

Quantum age of vector magnetic field meas.rements

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s.ience China(Phys.cs.Mechanics.& As.ronomy) 2024年第10期67卷 164-165页

作者： min Jiang CAs.Key Laboratory of Micros.ale Magnetic Res.nance and s.hool of Phys.cal s.iences University of Science and Technology of ChinaHefei 230026China

Magnetic fields.are ubiquitous.and precis. meas.rements.of them can s.rve as.powerful tools.for deep unders.anding and accurate detection acros. multiple dis.iplines.including phys.cs.biology,and chemis.ry[1].For ins.ance,by meas.ring the magnetic fields.within the human heart and brain,the medical community could obtain accurate dis.as. diagnos.s.

关键词： magnetic Quantum precis.

Novel ens.mble learning bas.d on multiple s.ction dis.ribution in dis.ributed environment

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Journal of s.s.ems.Engineering and Electronics 2008年第2期19卷 377-380页

作者： Fang min Ins.. of Computer Xidian Univ. Xi'an 710071 P. R. China

Becaus. mos. ens.mble learning algorithms.us. the centralized model, and the training ins.ances.mus. be centralized on a s.ngle s.ation, it is.difficult to centralize the training data on a s.ation. A dis.ributed ens.mble learning algorithm is.propos.d which has.two kinds.of weight genes.of ins.ances.that denote the global dis.ribution and the local dis.ribution. Ins.ead of the repeated s.mpling method in the s.andard ens.mble learning, non-balance s.mpling from each s.ation is.us.d to train the bas. clas.ifier s.t of each s.ation. The concept of the effective nearby region for local integration clas.ifier is.propos.d, and is.us.d for the dynamic integration method of multiple clas.ifiers.in dis.ributed environment. The experiments.s.ow that the ens.mble learning algorithm in dis.ributed environment propos.d could reduce the time of training the bas. clas.ifiers.effectively, and ens.re the clas.ify performance is.as.s.me as.the centralized learning method.

关键词： dis.ributed environment ens.mble learning multiple clas.ifiers.combination.

The compos.te X-ray s.ectra of radio-loud and radio-quiet s.s. quas.rs

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Res.arch in As.ronomy and As.rophys.cs 2021年第1期21卷 33-44页

作者： min-Hua Zhou min-Feng Gu Key Laboratory for Res.arch in Galaxies.and Cos.ology Shanghai Astronomical ObservatoryChinese Academy ofSciences80 Nandan RoadShanghai 200030China Univers.ty of Chines. Academy of s.iences 19A Yuquan RoadBeijing 100049China

We pres.nt a s.udy of the X-ray emis.ion for a s.mple of radio-detected quas.rs.cons.ructed from the cros.-matches.between s.s.,FIRs. catalogs.and XMM-Newton archives.A s.mple of radio-quiet s.s. quas.rs.without FIRs. radio detection is.als. as.embled for *** cons.ruct the optical and X-ray compos.te s.ectra normalized at res. frame 4215 A(or 2200 A)for both radio-loud quas.rs.RLQs.and radio-quiet quas.rs.RQQs.at z≤3.2,with matched X-ray completenes. of 19%,reds.ift and optical *** the optical compos.te s.ectrum of RLQs.is.s.milar to that of RQQs.we find that RLQs.have a higher X-ray compos.te s.ectrum than RQQs.cons.s.ent with previous.s.udies.in the *** dividing the radio-detected quas.rs.into radio loudnes. bins.we find the X-ray compos.te s.ectra are generally higher with increas.ng radio ***,a s.gnificant correlation is.found between the optical-to-X-ray s.ectral index and radio loudnes.,and there is.a unified multi-correlation between the radio and X-ray luminos.ties.and radio loudnes. in radio-detected *** res.lts.could be pos.ibly explained with the corona-jet model,in which the corona and jet are directly related.

关键词： methods.s.atis.ical catalogs quas.rs.general X-rays.general

Characteris.ics.of Pre-s.mmer Daytime Cloud Regimes.over Coas.al s.uth China from the Himawari-8 s.tellite

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Advances.in Atmos.heric s.iences 2022年第12期39卷 2008-2023,I0001-I0015页

作者： mingxin LI Yali LUO min min s.ate Key Laboratory of s.vere Weather Chinese Academy of Meteorological SciencesBeijing 100081China Collaborative Innovation Center on Forecas. and Evaluation of Meteorological Dis.s.ers Nanjing University of Information Science and TechnologyNanjing 210044China s.hool of Atmos.heric s.iences and Guangdong Province Key Laboratory for Climate Change and Natural Disaster StudiesSun Yat-sen UniversityGuangzhou 519082China

Us.ng the high s.atiotemporal res.lution(2 km-and-10 min)data from the Advanced Himawari Imager onboard the Himawari-8 s.tellite,this.s.udy documents.the fine-s.ale characteris.ics.of daytime cloud regimes.CRs.over coas.al s.uth China during the pre-s.mmer rainy s.as.n(April–June).s.x CRs.CR1–CR6)are identified bas.d on the joint frequency dis.ribution of cloud top brightnes. temperature and cloud optical thicknes.,namely,the optically thin-to-moderate cloud mixture,optically thin warm clouds.with cirrus.optically thick warm clouds.weak convective cloud mixture,s.rong convective clouds.and extreme,deep convective *** optically thick warm clouds.are the major CR during April and May,with higher frequencies.over land,es.ecially along the urban agglomeration,rather than the offs.ore which may be an indicator of the higher aeros.l concentrations.being a contributing factor over the *** CRs.with weak convective cloud mixtures.and s.rong convective clouds.appear more frequently over the land,while the two CRs.with optically thinner clouds.occur mainly *** flow patterns.s.s.are objectively identified and examined focus.ng on thos. favoring the two major rainproducing CRs.CR5 and CR6)and the highly reflective CR with optically thick warm clouds.CR3).The two s.s.favoring CR5 and CR6 are characterized by abundant mois.ure with low-level jets.after mons.on ons.t,and a northwes. highs.utheas. low pattern with s.rong dynamic convergence along the coas.line,*** non-convective CR3 with high reflectance is.related to a s. that features.the wes.ern North Pacific s.btropical high extending more wes.ward,leading to a moderate mois.ure s.pply and a wide range of convective available potential energy,but als.,large convective inhibition.

关键词： cloud regime mons.on ons.t s.noptic pattern s.tellite data s.atiotemporal dis.ribution

Effect of internal s.os.ing on added res.s.ance of s.ip

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Journal of Hydrodynamics 2017年第1期29卷 13-26页

作者： min-Guk s.o Yonghwan Kim Dong-min Park Korea Res.arch Ins.itute of s.ips.and Ocean Engineering DaejeonKorea Department of Naval Architecture and Ocean Engineering Seoul National UniversitySeoulKorea

The motion res.ons.s.of s.ips.carrying liquid cargo are affected not only by external wave excitation, but als. by internal s.os.ing-induced forces.and moments. s.os.ing flow is.coupled with the s.ip motion. This.means.the added res.s.ance in waves.may change when s.os.ing occurs.ins.de the tank of the s.ip. In this.s.udy, the motion res.ons.s.and added res.s.ance of a s.ip, coupled with the s.os.ing-induced internal forces.and moments.are cons.dered by us.ng the linear potential theory. The three-dimens.onal Rankine panel method, in which the phys.cal quantities.are repres.nted by us.ng B-s.line bas.s.function, is.applied. The s.os.ing flow of inner tanks.is.als. s.mulated by Us.ng the Rankine panel method and linearized boundary value problem. To s.udy the added res.s.ance, a near-field method, which integrates.the s.cond-order pres.ure on a body s.rface, is.applied. The model s.ip is.a blunt modified Wigley model with two inner tanks. Numerical res.lts.obtained without inner tanks.are compared with the experimental data, and then the effect of filling ratio of inner tanks.on s.ip motion and added res.s.ance are obs.rved. The components.that induce added res.s.ance are examined, and the effects.of s.rge motion on s.os.ing flow and added res.s.ance are briefly cons.dered. This.s.udy s.ows.that the s.os.ing flow ins.de the inner tanks.may s.gnificantly influence not only the motion res.ons.s. but als. added res.s.ance, es.ecially, when the incident wave frequency approaches.the res.nance frequency of the s.os.ing flow.

关键词： Added res.s.ance s.os.ing s.akeeping coupled effect Rankine panel method

Effect of Low-Pres.ure Nitrogen DC Plas.a Treatment on the s.rface Properties.of Biaxially Oriented Polypropylene, Poly (Methyl Methacrylate) and Polyvinyl Chloride Films

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Plas.a s.ience and Technology 2013年第4期15卷 362-367页

作者： s. Hamideh MORTAZAVI Mahmood GHORANNEVIs. s.heil PILEHVAR s.na Es.AEILI s.amim ZARGHAM s. Ebrahim HAs.EMI Hamzeh JODAT Plas.a Phys.cs.Res.arch Center Science and Research Branch Islamic Azad University Tehran Iran Polymer Engineering Group Composite Science and Technology Research CenterTehran Iran Plas.a Phys.cs.Res.arch Center Science and Research Branch Islamic Azad UniversityTehran Iran Polymer Engineering Center Science and Research Branch Islamic Azad UniversityTehran Iran Polymer Engineering Group Composite Science and Technology Research CenterTehran Iran

In this.s.udy, commercial biaxially oriented polypropylene （BOPP）, polyvinyl chlo- ride （PVC） and poly （methyl methacrylate）（PMMA） films.were treated with nitrogen plas.a over different expos.re times.in a Pyrex tube s.rrounded by a DC variable magnetic field. The chemi- cal changes.that appeared on the s.rface of the s.mples.were inves.igated us.ng Fourier trans.orm infrared （FT4R） s.ectros.opy and attenuated total reflectance Fourier trans.orm infrared （ATR- FTIR） s.ectros.opy after treatment for 2 min, 4 min and 6 rain in a nitrogen plas.a chamber. Effects.of the plas.a treatment on the s.rface topographies.and contact angles.of the untreated and plas.a treated films.were als. analyzed by atomic force micros.opy （AFM） and a contact angle meas.ring s.s.em. The res.lts.s.ow that the plas.a treated films.become more hydrophilic with an enhanced wettability due to the formation of s.me new polar groups.on the s.rface of the treated films. Moreover, at higher expos.re times. the total s.rface energy in all treated films.increas.d while a reduction in contact angle occurred. The behavior of s.rface roughnes. in each s.mple was.completely different at higher expos.re times.

关键词： plas.a s.rface treatment biaxially oriented polypropylene polyvinyl chlo-ride poly (methyl methacrylate) wettability

Twis.ed phonon polaritons.at deep s.bwavelength s.ale

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Light(s.ience & Applications. 2022年第9期11卷 1960-1961页

作者： Ren-min Ma s.ate Key Lab for Mes.s.opic Phys.cs.and s.hool of Phys.cs Peking UniversityBeijingChina Frontiers.s.ience Center for Nano-optoelectronics.Collaborative Innovation Center of Quantum Matter BeijingChina

Hyperbolic polariton vortices.carrying reconfigurable topological charges.have been realized at deep s.bwavelength s.ale.

关键词： polar phonon Hyperbolic