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Partial purification,immobilization and preliminary biochemical characterization of lipases from Rhizomucor pusillus

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Advances in Enzyme Research 2013年第4期1卷 79-90页

作者： Ana Lucia Ferrarezi Daniele H.Pivetta Gustavo Orlando Bonilla-Rodriguez Roberto da Silva Jose Manuel Guisan Eleni Gomes benevides costa pessela Department of Biology Laboratory of Applied Biochemistry and MicrobiologyIBILCE-UNESPSao Jose do Rio Preto SPBrazil Department of Chemistry and Environmental Sciences IBILCE-UNESPSao Jose do Rio Preto SPBrazil Department of Enzyme Biocatalysis Institute of Catalysis and PetrochemistryCSICCampus UAMCantoblancoMadridSpain Department of Biotechnology and Food Microbiology Research Institute for Food ScienceCIAL-CSICCalleNicolás Cabrera 9Campus UAMMadridSpain

Lipases have important applications in biotechnological processes, motivating us to produce, purify, immobilize and perform a biochemical characterization of the lipase from Rhizomucor pusillus. The fungus was cultivated by solid state fermentation producing lipolytic activity of about 0.5 U/mL(4U/g). A partial purification by gel filtration chromatography in Se-phacryl S-100 allowed obtaining a yield of about 85% and a purification factor of 5.7. Our results revealed that the purified enzyme is very stable with some significant differences in its properties when compared to crude extract. The crude enzyme extract has an optimum pH and temperature of 7.5 ° C and 40 ° C, respectively. After purification, a shift of the optimum pH from 7 to 8 was observed, as well as a rise in optimumtemperature to 60 ° C and an increase in stability. The enzyme was immobilized on CNBr-Agarose and Octyl-Agarose supports, having the highest immobilization yield of 94% in the second resin. The major advantage of immobilization in hydrophobic media such as Octyl is in its hyper activation, which in this case was over 200%, a very interesting finding. Another advantage of this type of immobilization is the possibility of using the derivatives in biotechnological applications, such as in oil enriched with omega-3 as the results obtained in this study display the hydrolysis of 40% EPA and 7% DHA from sardine oil, promising results compared to the literature.

关键词： Enzyme Immobilization Solid State Fermentation Purification n-3 Polyunsatured Fatty Acids Rhizomucor pusillus

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NEW ADVANCES IN NON-ORTHOGONAL MULTIPLE ACCESS

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China Communications 2020年第11期17卷 I0001-I0004页

作者： Miaowen Wen Wei Duan Zhiguo Ding Daniel benevides da costa Ioannis Krikidis Yifei Yuan Peking University BeijingChina Chonbuk National University JeonjuSouth Korea University of Manchester 不详 ZTE as technical

Multiple access is one of the core technologies in wireless communications,which enables wireless base stations to support a large number of terminal users and serve them simultaneously under stringent spectrum *** multiple access(OMA),as one multiple access method,has been prevailing since the first generation(1G)cellular ***,the number of active users allowed access to the OMA system is strictly limited by the number of available orthogonal resources,since as required by OMA the communication resources allocated to different users are orthogonal in at least one radio resource dimension,e.g.,frequency,time,code,***,with the rapid growth of mobile network and Internet of Things(IoT)this problem becomes more and more critical.

关键词： ACCESS prevailing enable

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Ambient Backscatter Communications over NOMA Downlink Channels

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China Communications 2020年第6期17卷 80-100页

作者： Weiyu Chen Haiyang Ding Shilian Wang Daniel benevides dacosta Fengkui Gong Pedro Henrique Juliano Nardelli College of Electronic Science and Technology National University of Defense TechnologyChangsha 410073China College of Information and Communication National University of Defense TechnologyXi’an 710106China Department of Computer Engineering Federal University of CearáSobral 62010-560Brazil State Key Laboratory of Integrated Service Networks Xidian UniversityXi’an 710071China School of Energy Systems Lappeenranta University of TechnologyLappeenranta 53850Finland

In this paper,we investigate the performance of commensal ambient backscatter communications(AmBC)that ride on a non-ortho go nal multiple access(NOMA)downlink transmission,in which a backscatter device(BD)splits part of its received signals from the base station(BS)for energy harvesting,and backscatters the remaining received signals to transmit information to a cellular ***,under the power consumption constraint at BD and the peak transmit power constraint at BS,we derive the optimal reflection coefficient at BD,the optimal total transmit power at BS,and the optimal power allocation at BS for each transmission block to maximize the ergodic capacity of the ambient backscatter transmission on the premise of preserving the outage performance of the NOMA downlink ***,we consider a scenario where the BS is restricted by a maximum allowed average transmit power and the reflection coefficient at BD is fixed due to BD’s low-complexity *** algorithm is developed to determine the optimal total transmit power and power allocation at BS for this ***,a low-complexity algorithm is proposed for this scenario to reduce the computational complexity and the signaling ***,the performance of the derived solutions are studied and compared via numerical simulations.

关键词： power-domain NOMA ambient backscatter communications IoT wireless-powered devices optimization

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Isolation and Screening of Filamentous Fungi Producing Extracellular Lipase with Potential in Biodiesel Production

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Advances in Enzyme Research 2015年第4期3卷 101-114页

作者： Sabrina Moro Villela Pacheco Américo Cruz Júnior Ayres Ferreira Morgado Agenor Furigo Júnior Onyetugo Chioma Amadi José Manuel Guisán benevides pessela Department of Food and Chemistry Engineering University Campus UFSC Florianópolis Brazil Department of Microbiology Faculty of Biological Science University of Nigeria Enugu Nigeria Departamento de Biocatálises Enzimática Instituto de Catalisis y Petroleoquimica ICP (CSIC-UAM) C/Marie Curie 2 Cantoblanco Campus de la Univerisdad Autónoma de Madrid Madrid Espa&ntilde a Instituto de Investigación en Ciencias de la Alimentación CIAL (CSIC-UAM) C/Nicolás Cabrera 9 Cantoblanco Campus de la Universidad Autónoma de Madrid Madrid Espa&ntilde a Departamento de Engenharia e Tecnologías Instituto Superior Politécnico de Tecnología e de Ciências ISPTEC Luanda Sul Talatona República de Angola

Nineteen fungal strains were isolated from a chicken slaughterhouse effluent and within those, only one showed high values of lipolytic activity in submerged cultures. This fungus was identified as Trametes hirsuta. The crude extract was immobilized in chitosan/clay beads, with an immobilization yield of 80.9%. The analyses of the crude extract and the immobilized derivative at different temperatures, pH (s), solvents, metallic ions and storage showed that the immobilization process increased the enzyme life span. Ethyl esters were obtained in solvent free systems using chicken viscera oil and the enzyme crude extract. For effective comparison, a reaction using viscera oil and commercial lipase Novozym 435 was carried out. The result revealed 35% and 28% esters conversion in the reactions containing chicken viscera oil, using Novozym 435 and the crude extract respectively. The extract was also used in a reaction with soybean oil, traditionally used as starting substrate for biodiesel production.

关键词： Biodiesel Production Trametes Hirsuta Lipase

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Energetic-protein supplementation in the last 60 days of gestation improves performance of beef cows grazing tropical pastures

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Journal of Animal Science and Biotechnology 2018年第1期9卷 208-216页

作者： Aline Gomes da Silva Mário Fonseca Paulino Edenio Detmann Henrique Jorge Fernandes Lincoln da Silva Amorim Román Enrique Maza Ortega Victor Valério de Carvalho Josilaine Aparecida da costa Lima Felipe Henrique de Moura Mariana benevides Monteiro Jéssika Almeida Bitencourt Universidade Federal de Vicosa VicosaMinas Gerais 36570-000Brazil Universidade Federal de Mato Grosso do Sul Campo GrandeMato Grosso do Sul 79074-460Brazil Universidade Estadual de Mato Grosso do Sul AquidauanaMato Grosso do Sul 79200-000Brazil Biotran Biotecnologia e Treinamento em Reproducao Animal AlfenasMinas Gerais 37130-000Brazil Universidade Federal do Acre Rio BrancoAcre 69920-900Brazil

Background： Nutrition is one of the most important factors that affect animal performance, and it therefore also impacts on financial results in beef systems. In this way, finding the best strategy for feeding supplements is of paramount importance. Aiming to evaluate the effect of supplement feeding strategies for beef cows in the last third of gestation, two experiments were conducted. In Experiment 1, 35 pregnant Nellore cows were assigned to a completely randomized design with four treatments： control, which received no supplement; supplementation for the last 30 d of gestation（30-d; 3.0 kg/d）; supplementation for the last 60 d of gestation（60-d; 1.5 kg/d）; or supplementation for the last 90 d of gestation（90-d; 1.0 kg/d）. All supplemented treatments received the same total amount of supplement throughout the experiment： 90 kg（20% of crude protein）. A second experiment（Experiment 2） was delineated to evaluate the effects of the amounts offered in Experiment 1 on intake and metabolism. Four multiparous pregnant Nellore cows were assigned to a 4 × 4 Latin square design, with periods of15 d ***： There was a linear effect of the number of days of supplementation on calving body weight（BW; P 〈 0.05）and a quadratic effect on BW change from parturition to d 31 post-calving（P 〈 0.05）, with cows on the 60-d strategy losing less BW post-calving. No difference was found in offspring birth BW（P 〉 0.10）. A significant linear effect on interval from parturition to conception（P 〈 0.05） was observed, with the highest calving to conception interval being observed in the 90-d strategy. The level of supplementation did not affect forage intake or neutral detergent fiber digestibility（P 〉 0.10）. Nitrogen excreted through urine tended to increase linearly with the level of supplementation（P 〈 0.10）.Conclusion： Providing 1.5 kg of supplement during the last 60 d of gestation improves cow performance after calving, reducing the magnitude of BW lost, and reduces the number of

关键词： Flushing Nutrition Parturition Reproduction

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Analysis of Nursing Care Concept: Perspective of the Person Submitted to Care

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Open Journal of Nursing 2016年第6期6卷 491-496页

作者： Camila Brasil Moreira Ana Fátima Carvalho Fernandes Míria Conceição Lavinas Santos Altamira Mendonça Félix Gomes Carla Monique Lopes Mourão Rosy Denyse Pinheiro de Oliveira Angelica Isabely de Morais Almeida Suzy Ramos Rocha Cláudia Rayanna Silva Mendes Sadrine Maria Eufrasino de Pinho Marinna Maria de Andrade costa Jordana Prado benevides Caroline Batista de Queiroz Aquino Iarlla Silva Ferreira Raquel Vilanova Araujo Department of Nursing Federal University of Ceará Fortaleza Brazil

This study aimed to analyze the concept of nursing care to patients with breast cancer on chemotherapy treatment, according to the perspective of the person submitted to care. The study followed the conceptual analysis methodology proposed by Walker and Avant according to Waldow, and it was developed through consultation to SCOPUS and CINAHL databases. The selection was carried out by inclusion and exclusion criteria, constituting a sample of 7 articles. There have been identified the main uses of the risk concept, defining attributes, antecedents and consequences of the concept. The evaluation of the concept and nursing care to breast cancer patient on chemotherapy treatment allowed a broader understanding of the phenomenon, with emphasis on the skills and competencies in nursing care dispensed to woman on chemotherapy treatment for breast cancer.

关键词： Nursing Breast Neoplasms Analysis Research Nursing Care Chemotherapy

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