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BAK1-mediated phosphorylation of canonical G protein alpha during flagellin signaling in Arabidopsis

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Journal of Integrative Plant Biology 2020年第5期62卷 690-701页

作者： Jiao Xue ben-qiang gong Xinran Yao Xiangjuan Huang Jian-Feng Li Guangdong Provincial Key Laboratory of Plant Resources State Key Laboratory of BiocontrolMOE Key Laboratory of Gene Function and RegulationSchool of Life SciencesSun Yat-sen UniversityGuangzhou 510275China

Heterotrimeric G proteins consisting of Gα,Gβand Gγare conserved signaling hubs in *** analogs to canonical animal G protein-coupled receptors,plant cells are thought to use RGS1 and a yet unknown mechanism to regulate the activity of Gα.Meanwhile,the exact role of canonical Gαin plant innate immunity remains ***,we report multiple immune deficiencies in the null allele of Arabidopsis Gα(GPA1)in response to bacterial flg22 elicitor,clarifying a positive regulatory role of GPA1 in flg22 *** also detect overall increased phosphorylation of GPA1 but reduced phosphorylation at Thr^19 upon flg22 ***,flg22 could not induce phosphorylation of GPA1 T19 Aand GPA1 T19D,suggesting that the dynamic Thr^19 phosphorylation is required for GPA1 to respond to ***,flg22-induced GPA1 phosphorylation is largely abolished in the absence of BAK1 in vivo,and BAK1 could phosphorylate GPA1 but not GPA1^T19A in vitro at the phosphorylation sites identified in vivo,suggesting BAK1 is likely the kinase for GPA1 phosphorylation in response to ***,the T19A mutation could promote flg22-induced association,rather than dissociation,between GPA1 and *** together,our findings shed new insights into the function and regulation of GPA1 in Arabidopsis defense signaling.

关键词： phosphorylation Arabidopsis immunity

Split Nano luciferase complementation for probing protein-protein interactions in plant cells

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Journal of Integrative Plant Biology 2020年第8期62卷 1065-1079页

作者： Feng-Zhu Wang Nannan Zhang Yan-Jun Guo ben-qiang gong Jian-Feng Li Guangdong Provincial Key Laboratory of Plant Resources State Key Laboratory of BiocontrolMOE Key Laboratory of Gene Function and RegulationSchool of Life SciencesSun Yat‐sen UniversityGuangzhou 510275China Guangdong Provincial Key Laboratory of Sugarcane Improvement and Biorefinery Guangdong Bioengineering InstituteGuangzhou 510316China

Deciphering protein-protein interactions(PPIs)is fundamental for understanding signal transduction pathways in *** split firefly luciferase(Fluc)complementation(SLC)assay has been widely used for analyzing ***,concern has risen about the bulky halves of Fluc interfering with the functions of their fusion *** luciferase(Nluc)is the smallest substitute for Fluc with improved stability and ***,we developed a dual-use system enabling the detection of PPIs through the Nluc-based SLC and co-immunoprecipitation *** was realized by coexpression of two proteins under investigation in fusion with the HA-or FLAG-tagged Nluc halves,*** validated the robustness of this system by reproducing multiple previously documented PPIs in protoplasts or Agrobacterium-transformed *** next applied this system to evaluate the homodimerization of Arabidopsis CERK1,a coreceptor of fungal elicitor chitin,and its heterodimerization with other homologs in the absence or presence of ***,split fragments of Nluc were fused to two cytosolic ends of Arabidopsis calcium channels CNGC2 and CNGC4 to help sense the allosteric change induced by the bacterial elicitor ***,these results demonstrate the usefulness of the Nluc-based SLC assay for probing constitutive or inducible PPIs and protein allostery in plant cells.

关键词： system interactions Arabidopsis

Modular assembly of complex C-alkyl glycoside enabled via cooperative NHC and photoredox catalysis

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Science China Chemistry 2025年

作者： Rui-qiang Jiao Ming Li Xi Chen Mei-Ze Xu Tian Ding Xiao-Ping gong Zhuo-Mei Li Xue-Yuan Liu Yong-Min Liang State Key Laboratory of Applied Organic Chemistry Lanzhou University College of Chemistry and Chemical Engineering Northwest Normal University

C-Alkyl glycosides are found in natural products and are indispensable in drug development programs,owing to their diverse range of medicinal ***-component reactions involving glycosyl radical addition are well documented and proved efficient for the C-alkyl glycosides ***,multicomponent reactions for the rapid and controllable construction of structurally diversified C-alkyl glycosides in a single step remains a significant challenge due to the issues associated with balancing reactivity,compatibility,and ***,we report an NHC and photoredox co-catalyzed three-component method to access complex and valuable C-alkyl *** protocol exhibits broad substrate scope and excellent chemoand ***,nine types of sugars are compatible with this *** addition,the generality of this catalytic strategy was further highlighted by the potential for scalable production,the feasibility of further modifications of the products and its successful application in the late-stage functionalization of pharmaceutical skeletons.

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Polymeric partners breathe together: using gas to direct polymer self-assembly via gas-bridging chemistry

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Science China Chemistry 2022年第7期65卷 1401-1410页

作者： Zehao gong Yixin Wang qiang Yan State Key Laboratory of Molecular Engineering of Polymers Department of Macromolecular ScienceFudan University200433China

The quest for a general and facile way to regulate polymer self-assembled nanostructures with low-to high-order ergodicity is an eternal theme in soft nanoparticle fabrication. Here we present an unprecedented gas-bridging strategy that allows to use gas to direct polymer self-assembly in continuous and tunable manners. Such system comprises a partner of frustrated Lewis polymers with bulky Lewis acid and base groups. They can together “breathe in” external gases to form gas-bridged structures between the two complementary moieties, which drive their mutual complexation and assemble into polymer nanoparticles of diverse geometries and dimensionalities. This strategy is applicable to a broad family of gas substances including but not limited to carbon oxides, nitrogen oxides, sulfur oxides, and even olefins;moreover, tailoring gas types and levels can dictate distinct assembling evolutionary pathways and deformable behaviors among spherical, fibrous, polymersomal, tubesomal and cubosomal morphologies. We also discover that the gas-based bonding chemistry is the mechanistic basis underlying the phase transitional control and phase window regulation. This will open a new direction of making bespoke polymer nanostructures with gas.

关键词： gas-bridging bond Lewis pair polymer dynamic covalent chemistry self-assembly tunable nanostructure

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Photoregulated supramolecular hydrogels driven by polyradical interactions

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Chinese Chemical Letters 2023年第7期34卷 340-344页

作者： Zehao gong qiang Yan State Key Laboratory of Molecular Engineering of Polymers and Department of Macromolecular Science Fudan UniversityShanghai 200433China

Organic radical as a powerful tool has been extensively applied in synthetic chemistry. However, harnessing radical-mediated noncovalent interactions to fabricate soft materials remains elusive. Here we report a new category of supramolecular hydrogel system held by multiple radical-radical(polyradical) interactions, and its photosensitive cross-linking structure. A simple polyacrylamide with triarylamine(TAA)pendants is designed as the precursor. The TAA units in polymer can be converted into active TAA^(·+)radical cations with light and further associate each other via TAA^(·+)–TAA^(·+)stacking interactions to form stable supramolecular network. Temporal control of the light irradiation dictates the degree of radical stacks, thus regulating the mechanical performance of the resulting hydrogel materials on-demand. Moreover, the reversible collapse of this hydrogels can be promoted by adding radical scavenger or exerting reduction voltage.

关键词： Radical-radical interactions Polyradical Supramolecular hydrogel Polymer network Photoresponsiveness

Estimation of multipath parameters in wireless communications using multi-way compressive sensing

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Journal of Systems Engineering and Electronics 2015年第5期26卷 908-915页

作者： Fangqing Wen gong Zhang De ben College of Electronics and Information Engineering Nanjing University of Aeronautics and Astronautics Key Laboratory of Radar Imaging and Microwave Photonics Ministry of Education Nanjing Research Institute of Electronics Technology

This paper addresses the problem of joint angle and delay estimation(JADE) in a multipath communication scenario. A low-complexity multi-way compressive sensing(MCS) estimation algorithm is proposed. The received data are firstly stacked up to a trilinear tensor model. To reduce the computational complexity,three random compression matrices are individually used to reduce each tensor to a much smaller one. JADE then is linked to a low-dimensional trilinear model. Our algorithm has an estimation performance very close to that of the parallel factor analysis(PARAFAC) algorithm and automatic pairing of the two parameter sets. Compared with other methods, such as multiple signal classification(MUSIC), the estimation of signal parameters via rotational invariance techniques(ESPRIT), the MCS algorithm requires neither eigenvalue decomposition of the received signal covariance matrix nor spectral peak searching. It also does not require the channel fading information, which means the proposed algorithm is blind and robust, therefore it has a higher working *** results indicate the proposed algorithm have a bright future in wireless communications.

关键词： multipath channel delay estimation direction-ofarrival(DOA) estimation multi-way compressive sensing(MCS)

1+1>2: Learning from the interfacial modulation on single-atom electrocatalysts to design dual-atom electrocatalysts for dinitrogen reduction

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Green Energy & Environment 2023年第6期8卷 1753-1763页

作者： qiang Zhou Feng gong Yunlong Xie Rui Xiao College of Materials Xiamen UniversityXiamen361005China Key Laboratory of Energy Thermal Conversion and Control of Ministry of Education School of Energy and EnvironmentSoutheast UniversityNanjing211189JiangsuChina Institute for Advanced Materials Hubei Normal UniversityHuangshi435002China

Developing efficient electrocatalysts for converting dinitrogen to ammonia through electrocatalysis is of significance to the decentralized ammonia production. Here, through high-throughput density functional theory calculations, we demonstrated that the interfacial modulation of hexagonal boron nitride/graphene(hBN-graphene) could sufficiently improve the catalytic activity of the single transition metal atom catalysts for nitrogen reduction reaction(NRR). It was revealed that Re@hBN-graphene and Os@hBN-graphene possessed remarkable NRR catalytic activity with low limiting potentials of 0.29 V and 0.33 V, respectively. Furthermore, the mechanism of the enhanced catalytic activity was investigated based on various descriptors of the adsorption energies of intermediates, where the synergistic effect of hBN and graphene in the hybrid substrate was found to play a key role. Motivated by the synergistic effect of hybrid substrate in single-atom catalysts, a novel strategy was proposed to efficiently design dual-atom catalysts by integrating the merits of both metal components. The as-designed dual-atom catalyst Fe-Mo@hBN exhibited more excellent NRR catalytic performance with a limiting potential of 0.17 V, manifesting the solidity of the design strategy. Our findings open new avenues for the search of heterostructure substrates for single-atom catalysts and the efficient design of dualatom catalysts for NRR.

关键词： Nitrogen reduction reaction Boron nitride Graphene High throughput Descriptor Density functional theory Single-atom catalyst Dual-atom catalyst

Local parameter identification with neural ordinary differential equations

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Applied Mathematics and Mechanics(English Edition) 2022年第12期43卷 1887-1900页

作者： qiang YIN Juntong CAI Xue gong Qian DING Department of Mechanics Tianjin UniversityTianjin 300350China

The data-driven methods extract the feature information from data to build system models, which enable estimation and identification of the systems and can be utilized for prognosis and health management(PHM). However, most data-driven models are still black-box models that cannot be interpreted. In this study, we use the neural ordinary differential equations(ODEs), especially the inherent computational relationships of a system added to the loss function calculation, to approximate the governing equations. In addition, a new strategy for identifying the local parameters of the system is investigated, which can be utilized for system parameter identification and damage detection. The numerical and experimental examples presented in the paper demonstrate that the strategy has high accuracy and good local parameter identification. Moreover, the proposed method has the advantage of being interpretable. It can directly approximate the underlying governing dynamics and be a worthwhile strategy for system identification and PHM.

关键词： neural ordinary differential equation(ODE) parameter identification prognosis and health management(PHM) system damage detection

Tunable Negative Poisson’s Ratio in Van der Waals Superlattice

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Research 2021年第1期2021卷 50-60页

作者： Xiaowen Li Xiaobin qiang Zhenhao gong Yubo Zhang Penglai gong Lang Chen Department of Physics Southern University of Science and TechnologyShenzhenGuangdong 518055China

Negative Poisson’s ratio(NPR)materials are functional and mechanical metamaterials that shrink(expand)longitudinally after being compressed(stretched)*** using first-principles calculations,we found that Poisson’s ratio can be tuned from near zero to negative by different stacking modes in van der Waals(vdW)graphene/hexagonal boron nitride(G/h-BN)*** attribute the NPR effect to the interaction of pz orbitals between the interfacial ***,a parameter calculated by analyzing the electronic band structure,namely,distance-dependent hopping integral,is used to describe the intensity of this *** believe that this mechanism is not only applicable to G/h-BN superlattice but can also explain and predict the NPR effect in other vdW layered ***,the NPR phenomenon,which was relatively rare in 3D and 2D materials,can be realized in the vdW superlattices by different stacking *** combinations of tunable NPRs with the excellent electrical/optical properties of 2D vdW superlattices will pave a novel avenue to a wide range of multifunctional applications.

关键词： stacking hexagonal boron