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Expression of the Grifola frondosa Trehalose Synthase Gene and Improvement of Drought-Tolerance in Sugarcane （Saccharum officinarum L.）

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Journal of Integrative Plant Biology 2006年第4期48卷 453-459页

作者： Shu-Zhen Zhang ben-peng Yang Cui-Lian Feng Ru-Kai Chen Jing-Ping Luo Wen-Wei cai Fei-Hu Liu State Key Laboratory of Tropical Crop Biotechnology Chinese Academy of Tropical Agricultural Sciences Haikou 571101 China Institute of Tropical Biotechnology Chinese Academy of Tropical Agricultural Sciences Haikou 571101 China Key Laboratory of Ecophysiology and Genetic Improvement for Sugarcane Ministry of Agriculture Fuzhou 350002 China College of Life Sciences Yunnan University Kunming 650091 China

Trehalose Is a nonreduclng dlsaccharlde of glucose that functions as a protectant In the stabilization of blologlcal structures and enhances stress tolerance to abiotic stresses in organisms. We report here the expression of a Grlfola frondosa trehalose synthase （TSase） gene for Improving drought tolerance In sugarcane （Saccharum offlclnarum L.）. The expression of the transgene was under the control of two tandem copies of the CaMV35S promoter and transferred Into sugarcane by Agrobacterium tumefaciens EHA105. The transgenlc plants accumulated high levels of trehalose, up to 8.805-12.863 mg/g fresh weight, whereas It was present at undetectable level in nontransgenlc plants. It has been reported that transgenlc plants transformed with Escherlchla coil TPS （trehalose-6-phosphatesynthase） and/or TPP （trehalose-6-phosphate phosphatase） are severely stunted and have root morphologlc alterations. Interestingly, our transgenlc sugarcane plants had no obvious morphological changes and no growth Inhibition in the field. Trehalose accumulation in 35S-35S：TSase plants resulted In In- creased drought tolerance, as shown by the drought and the drought physiological Indexes, such as the rate of bound water/free water, plasma membrane permeability, malondlaldehyde content, chlorophyll a and b contents, and activity of SOD and POD of the excised leaves. These results suggest that transgenlc plants transformed with the TSase gene can accumulate high levels of trehalose and have enhanced tolerance to drought.

关键词： drought tolerance Grifola frondosa Saccharum officinarum trehalose synthase gene.

Overlooked risk for needle tract seeding following endoscopic ultrasound-guided minimally invasive tissue acquisition

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World Journal of Gastroenterology 2020年第40期26卷 6182-6194页

作者： Ruo-Yu Gao ben-Hua Wu Xin-Ying Shen Tie-Li peng De-Feng Li Cheng Wei Zhi-Chao Yu Ming-Han Luo Feng Xiong Li-Sheng wang Jun Yao Department of Gastroenterology Second Clinical Medical College of Jinan UniversityShenzhen People’s HospitalShenzhen 518020Guangdong ProvinceChina Department of Interventional Medicine Second Clinical Medical College of Jinan UniversityShenzhen People’s HospitalShenzhen 518020Guangdong ProvinceChina Department of Gastroenterology Institute of Digestive Disease of Guangzhou Medical UniversityThe Sixth Affiliated Hospital of Guangzhou Medical UniversityQingyuan 511500Guangdong ProvinceChina

Endoscopic ultrasound-guided minimally invasive tissue acquisition can be performed by two approaches as follows: Endoscopic ultrasound-guided fineneedle aspiration(EUS-FNA) and endoscopic ultrasound-guided fine-needle biopsy(EUS-FNB). These have been evolved into leading approaches and widely used for the histological diagnosis of tumors in the gastrointestinal tract and adjacent organs. However, the role of EUS-FNA and EUS-FNB in disease diagnosis and evaluation remains controversial. Although the incidence of surgery-associated complications remains low, the consequences of needle tract seeding can be serious or even life-threatening. Recently, increasing case reports of needle tract seeding are emerging, especially caused by EUS-FNA. This complication needs serious consideration. In the present work, we integrated these case reports and the related literature, and summarized the relevant cases and technical characteristics of needle tract seeding caused by EUS-FNA and EUSFNB. Collectively, our findings provided valuable insights into the prevention and reduction of such serious complication.

关键词： Endoscopic ultrasound-guided fine-needle aspiration Endoscopic ultrasoundguided fine-needle biopsy Needle tract seeding Gastrointestinal tract Computed tomography

Semi-global leader-following consensus-based formation flight of unmanned aerial vehicles

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Chinese Journal of Aeronautics 2022年第1期35卷 31-43页

作者： Panpan ZHOU ben M.CHEN Department of Mechanical ami Automation Engineering The Chinese University of Hong KongHong KongChina

In this paper,we investigate a formation control problem of multi-agent systems(specifically a group of unmanned aerial vehicles)based on a semi-global leader-following consensus approach with both the leader and the followers subject to input *** the low gain feedback design technique,a distributed static control protocol and a distributed adaptive control protocol are *** former solves the problem under an assumption that the communication network is undirected,and it depends on the global information of the *** the latter,we relax the undirected graph to directed ***,an adaptive updating gain is designed to avoid using the global information of the communication *** is shown that the consensus protocols can solve the semi-global leader-following consensus problem if the leader agent is globally *** results are verified successfully by both simulation and real flight tests.

关键词： Consensus Formation flying Input saturation Low gain feedback Multi-agent systems Unmanned aerial vehicles

Efficacy and safety of single microneedle radiofrequency *** therapy on moderate-to-severe acne vulgaris:A prospective,randomized,controlled study

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Chinese Medical Journal 2024年第8期137卷 1006-1008页

作者： Chuchu Huang Mengping Mao Yiran peng ben wang Yuxuan Deng Hongfu Xie Dan Jian Department of Dermatology Xiangya HospitalCentral South UniversityChangshaHunan 410008China National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangshaHunan 410008China Hunan Key Laboratory of Aging Biology Xiangya HospitalCentral South UniversityChangshaHunan 410008China Department of Dermatology and Allergy and Christine Kühne-Center for Allergy Research and EducationUniversity Hospital of BonnD-53127BonnGermany

To the Editor:Acne vulgaris is a prevalent skin disorder affecting both physical and psychosocial health.[1]Although photodynamic therapy(PDT)exists for moderate-to-severe acne,due to its adverse reactions,we are still looking forward to a novel therapeutic option with fewer side ***,radiofrequency has been used to treat moderate acne.[2]In this study,we aimed to evaluate the efcacy and safety of moderate-to-severe acne treatment based on a single microneedle with radiofrequency(MRF)*** during three consecutive sessions through a prospective,randomized,and controlled trial study.

关键词： needle prospective randomized

基于降雨预报信息的梯级水电站不确定优化调度研究Ⅱ：耦合短、中期预报信息

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水利学报 2013年第8期44卷 1189-1196,1203页

作者：徐炜彭勇张弛王本德大连理工大学建设工程学部水利工程学院辽宁大连116024

基于聚合分解思想将梯级水库群聚合为"虚拟单库",降低了随机动态规划(SDP)模型的计算量,从而使SDP模型可以考虑更多预报信息。为了更近一步充分利用预报信息和降低预报信息不确定性对调度的影响,将降雨预报和径流预报的不确定性考虑为... 详细信息

基于聚合分解思想将梯级水库群聚合为"虚拟单库",降低了随机动态规划(SDP)模型的计算量,从而使SDP模型可以考虑更多预报信息。为了更近一步充分利用预报信息和降低预报信息不确定性对调度的影响,将降雨预报和径流预报的不确定性考虑为随预见期延长而不断增加的过程。首先将浑江流域的10d预报径流划分为前5d和后5d,并将前5d预报径流视为较准确的部分,而后5d预报径流视为不确定性较大部分;然后建立短、中期径流预报信息相套接的分段聚合分解贝叶斯随机动态规划(Two-Step-BSDP,TS-BSDP),为浑江梯级水库群制定前5d、后5d和10d的预报调度图;最后以5d为调度时段分别决策未来5d发电和10d发电计划。模拟调度结果表明,该模型中决策长度为5d的滚动决策具有更好的效果,该决策方式充分地利用了预报信息并有效地提高了发电效益。

关键词：水库群数值降雨预报预报信息套接聚合分解贝叶斯随机动态规划

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A review of studies of mechanism and prediction of tip vortex cavitation inception

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Journal of Hydrodynamics 2015年第4期27卷 488-495页

作者：张凌新张娜彭晓星王本龙邵雪明 State Key Laboratory of Fluid Power Transmission and Control Department of Mechanics Zhejiang University China Ship Scientific Research Center School of Naval Architecture Ocean and Civil Engineering Shanghai Jiao Tong University

The inception of the tip vortex cavitation（TVC） is a very important problem in cavitation researches. The study of the mechanism of the TVC inception is not only conducive to its prediction, but also helps to suppress or suspend the occurrence of cavitation. In this paper, the research progresses on the TVC inception including theoretical, experimental and numerical studies mainly in the last two decades are reviewed. It is shown that the TVC inception is affected by complicated factors, such as the water quality, the average pressure and the fluctuating pressure. In the scaling law for the determination of the TVC inception, all these factors are considered. To precisely describe the scaling law, more investigations are needed to understand the effects of the water quality and the fluctuating pressure.

关键词： tip vortex cavitation inception scaling law

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基于PIV速度场测量的压强梯度计算

航空动力学报 2017年第6期32卷 1491-1504页

作者：王勇陈鹏黄奔郑谢中国空气动力研究与发展中心空气动力学国家重点实验室四川绵阳621000 中国空气动力研究与发展中心气动噪声控制重点实验室四川绵阳621000

介绍了基于PIV速度场测量结果计算压强梯度的基本原理和3种不同的计算方法,并通过对流高斯涡模型系统研究了离散格式选取、PIV参数设置和流场特征等对压强梯度计算的影响.结果表明:PIV速度测量的精度是压强梯度计算的关键性影响因素,1%... 详细信息

介绍了基于PIV速度场测量结果计算压强梯度的基本原理和3种不同的计算方法,并通过对流高斯涡模型系统研究了离散格式选取、PIV参数设置和流场特征等对压强梯度计算的影响.结果表明:PIV速度测量的精度是压强梯度计算的关键性影响因素,1%噪声对结果的影响比其他过程高约2个数量级;时间分辨率和空间分辨率的设置并非越高越好,而是有个合适的中间值;拉格朗日方法在大多数情况下优于欧拉方法,但当流动结构较复杂时性能较差,应根据流动类型和流场特征合理选取压强梯度计算方法.

关键词：粒子图像测速(PIV) 压强梯度离散格式压强估计远场噪声预测

Revisiting an ancient inorganic aggregation-induced emission system:An enlightenment to clusteroluminescence

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Aggregate

Aggregate 2021年第2期2卷 127-135页

作者： Zheng Zhao Zaiyu wang Javad Tavakoli Guogang Shan Jianyu Zhang Chen peng Yu Xiong Xuepeng Zhang Tsz Shing Cheung Youhong Tang Bolong Huang Zhaoxun Yu Jacky W.Y.Lam ben Zhong Tang Department of Chemistry Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction and Institute for Advanced StudyThe Hong Kong University of Science and TechnologyClear Water BayKowloonHong Kong SARChina School of Chemistry and Chemical Engineering Southeast UniversityNanjingChina Centre for Health Technologies School of Biomedical EngineeringUniversity of Technology SydneySydneyNew South WalesAustralia HKUST Shenzhen Research Institute ShenzhenGuangdongChina College of Science and Engineering Flinders UniversityAdelaideSouth AustraliaAustralia Department of Applied Biology and Chemical Technology The Hong Kong Polytechnic UniversityHung HomKowloonHong Kong SARChina Micro-X Ltd TonsleySouth AustraliaAustralia Guangdong-Hong Kong-Macao Joint Laboratory of Optoelectronic and Magnetic Functional MaterialsGuangdong-Hong Kong-Macao China

Organic and inorganic clusteroluminescence have attracted great attention while the underlying mechanisms is still not well ***,we employed a series of ancient inorganic complexes platinocyanides with aggregation-induced emission property to elucidate the mechanism of clusteroluminescence including how does the chromophore form and how does the solid structures influence the luminescence *** results indicate that the isolated platinocyanide cannot work as a chromophore to emit visible light,while their clusterization at aggregate state can trigger the d-orbitals coupling of the platinum atoms to facilitate the electron exchange and delocalization to form a new chromophore to emit visible ***,the counter ions and H2O ligands help to rigidify the three-dimensional network structure of the platinocyanides to suppress the excited state nonradiative decay,resulting in the high quantum yield of up to 96%.This work fundamentally helps understanding both the organic and inorganic clusteroluminescence phenomenon.

关键词： aggregation-induced emission clusteroluminescence radioluminescence

Molecular Weight and Distribution of Cellulose and Cellulose Microspheres

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Agricultural Biotechnology 2012年第1期1卷 44-46页

作者： Zefen wang Kelin HUANG ben wang Rui WU Jing TAO Xiaoyu peng Dankui LIAO School of Chemistry and Chemical Engineering Guangxi University Guangxi Branch of China Science and Technology Development Institute Guangxi Research Institute of Chemical Industry Guangxi Hong Yao Xiang Technology Co. Ltd. School of Chemistry and Ecological Engineering Guangxi University for Nationalities

[ Objective ] This study aimed to provide basic data for studying the relationship between structure and property of cellulose microspheres by measuring molecular weight of cellulose and cellulose microspheres with viscosity method and gel permeation chromatography （GPC） method. [ Method] In viscosity method, cadmium ethylenediamine was used as the solvent, intrinsic viscosity η of the solution was determined at 25 ℃ by using a Ubbelohde viscometer, to calculate the molecular weight of cellulose; in GPC method, 8% LiC1 / N, N-dimethylacetamide （LiC1/DMAc） was used as the solvent and 0.5% LiC1/DMAc was used as the mobile phase to determine the relative molecular weight and distribution of cellulose and cellulose microspheres. In addition, the determination results were analyzed to compare these two methods. [ Result ] Viscosity-average molecular weight Mr/ of cellulose and cellulose microspheres determined with viscosity method were 224,532 and 16,686, respectively; weight-average molecular weight Mw of cellulose and cellulose microspheres determined with GPC method were 284,196 and 22,345, respectively. [ Conclusion] The determination results of （;PC method are relatively close to the actual value and could truly reflect the characteristics of molecular weialat distribution of eellulose and cellulose mierosr, heres.

关键词： Cellulose microspheres Viscosity method GPC method Molecular weight Molecular weight distribution