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Development of new ionic gelation strategy： Towards the preparation of new monodisperse and stable hyaluronic acid/ β-cyclodextrin-grafted chitosan nanoparticles as drug delivery carriers for doxorubicin

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Frontiers of Materials Science 2018年第1期12卷 83-94页

作者： Amina ben MIHOUB Boubakeur SAIDAT Youssef BAl Celine FROCHOT Regis VANDERESSE Samir ACHERAR laboratoire de Chimie Physique Macromoleculaire (lCPM) Universite de Lorraine-CNRS UMR 7375 1 Rue Grandville BP 2045154001 Nancy Cedex France laboratory of Physical Chemistry of Materials (lPCM) Faculty of Sciences (UATL) BP 37G Laghouat 03000 Algeria laboratory of Biomaterials & Transport Phenomena (lBTP) Quartier Ain D'Heb 26000 Medea Algeria Department of Chemistry Faculty of Sciences University Saed Dahleb of Blida (USDB) route de Soumae 09000 Blida Algeria laboratoire Reactions et Genie des Procedes (lRGP) Universite de Lorraine-CNRS UMR 7274 1 Rue Grandville BP 20451 54001 Nancy Cedex France

In the present study, β-cyclodextrin-grafted chitosan nanoparticles （β-CD- g-CS NPs） were prepared using a new ionic gelation strategy involving a synergistic effect of NaCI （150 mmol/l）, 4-（2-hydroxyethyl）-1-piperazineethanesulfonic acid （HEPES, 10 mmol/l）, and water bath sonication. This new strategy afforded smaller and more monodisperse β-CD-g-CS NPs vs. the classical ionic gelation method. New HA8β-CD-g-CS NPs were also prepared using the above-mentioned strategy by adding hyaluronic acid （HA） to the β-CD-g-CS copolymer at different weight ratios until the ZP values conversion. The best result was obtained with the weight ratio of w（HA）：w（β-CD-g-CS） = 2：1 and furnished new spherical and smooth HA/β-CD-g-CS NPs. Furthermore, the stability of β- CD-g-CS NPs and HA/β-CD-g-CS NPs at 4℃ in physiological medium （pH 7.4） was compared for 3 weeks period and showed that HA/β-CD-g-CS NPs were more stable all maintaining their monodispersity and high negative ZP values compared to β-CD-g-CS NPs. Finally, preliminary study of HA/β-CD-g-CS NPs as carrier for the controlled release of the anticancer drug doxorubicin was investigated. These new HA/β-CD-g-CS NPs can potentially be used as drug delivery and targeting systems for cancer treatment.

关键词： β-cyclodextrin-grafted chitosan hyaluronic acid ionic gelation drugdelivery physicochemical parameters control

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Photoelectrochemical activity of ZnO:Ag/rGO photo-anodes synthesized by two-steps sol-gel method

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Chinese Physics B 2022年第5期31卷 691-702页

作者： D ben Jemia M Karyaoui M A Wederni A Bardaoui M V Martinez-Huerta M Amlouk R Chtourou laboratory of Nanomaterials and Renewable Energy Systems lANSER Research and Technology Center of EnergyBorj-Cedria Science and Technology ParkBP 952050 Hammam-LifTunisia Faculty of Science of Tunis University of Tunis El ManarTunisia laboratory of Physics of Materials and Nanomaterials Applied to the Environment(laPhyMNE) Faculty of Sciences of GabesCite ErriadhUniversity of Gabes6079 GabesTunisia lnstitute of Catalysis and Petrochemistry CSICin MadridSpain laboratory of Nanomaterials Nanotechnology and Energy(L2NE)University of Tunis El ManarTunisia

This work investigated the influence of silver plasmon and reduced graphene oxide(r GO)on the photoelectrochemical performance(PEC)of Zn O thin films synthesized by the sol-gel *** physicochemical properties of the obtained photo-anodes were systematically studied by using several characterization *** x-ray diffraction analysis showed that all samples presented hexagonal wurtzite structure with a polycrystalline *** and energy dispersive x-ray(EDX)studies confirmed the existence of both Ag and r GO in Zn O:Ag/r GO thin *** estimated grain size obtained from scanning electron microscopy(SEM)analysis decreased with Ag doping,then increased to a maximum value after r GO *** UV-vis transmission spectra of the as-prepared Zn O:Ag and Zn O:Ag/r GO thin films have shown a reduction in the visible range with a redshift at the absorption *** bandgaps were estimated to be around 3.17 e V,2.7 e V,and 2.52 e V for Zn O,Zn O:Ag,and Zn O:Ag/r GO,***,the electrical measurements revealed that the charge exchange processes were enhanced at the Zn O:Ag/r GO/electrolyte interface,accompanied by an increase in the(PEC)performance compared to Zn O and Zn O:Ag ***,the photocurrent density of Zn O:Ag/r GO(0.2 m A·cm^(-2)) was around 4 and 2.22 times higher than photo-anodes based on undoped Zn O(0.05 m A·cm^(-2)) and Zn O:Ag(0.09 m A·cm^(-2)),***,from the flat band potential and donor density,deduced from the Mott-Schottky,it was clear that all the samples were n-type semiconductors with the highest carrier density for the Zn O:Ag/r GO photo-anode.

关键词： zinc oxide reduced graphene oxide silver doping sol-gel photoelectrochemical performance

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Special issue on control theory and applications in honor of the 60th birthday of Professor Frank l. lewis

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控制理论与应用（英文版） 2010年第3期8卷 259-261页

作者： Guest editors:ben M. CHEN Gang FENG

It is our great pleasure to put up this special issue in Journal of Control Theory and Applications in honor of the 60th birthday of Professor Frank lewis, who has made many significant contributions to the field of control engineering through the years, and who is regarded as a pioneer in many areas in control and automation, which includes applied optimal control and geometric systems theory in his earlier research period, and his recent focus on intelligent nonlinear control,

关键词： Special issue on control theory and applications in honor of the 60th birthday of Professor Frank l lewis

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Highly Sensitive and Selective Chemosensor for Cu2＋ Based on a Schiff Base

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Chemical Research in Chinese Universities 2013年第3期29卷 419-423页

作者： CAO ben-nan HU Qin HUANG Yan JIA Chun-man ZHANG Qi Key Study Center of Ministry of Education for Tropical Resources Utilization Hainan Provincial Key Lab of Fine Chemicals Hainan University Haikou 570228 P. R. China Department of Chemistry and Chemical Engineering Jiangsu University Jiangsu 212013 P. R. China

A new chemosensor based on a Schiff base has been designed and synthesized. Its sensing behavior toward various metal ions was investigated by fluorescence and UV-Vis spectroscopic methods. The fluorescence of the sensor was quenched and the color rapidly changed from canary yellow to brown after the addition of Cu2＋, while no changes occurred after the addition of other metal ions, which contributes to the detection of Cu2＋ with naked eyes. The fluorescence quantum yield of the ligand was calculated to be 0.52. The corresponding detection limit of Cu2＋ was 5.721 × 10-7 mol/l, and the 1：1 binding mode of the sensor with Cu2＋ was revealed by Job＇s plot.

关键词： Chemosensor Schiffbase Copper ion Naked-eye detection Quench

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Botryosphaeria, Neofusicoccum, Neoscytalidium and Pseudofusicoccum species associated with mango in Brazil

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Fungal Diversity 2013年第4期61卷 195-208页

作者： Marília W.Marques Nelson B.lima Marcos A.de Morais Jr Sami J.Michereff Alan J.l.phillips Marcos P.S.Câmara Departamento de Agronomia Universidade Federal Rural de Pernambuco52171-900 RecifeBrazil Departamento de Genética Universidade Federal de Pernambuco50732-970 RecifeBrazil Centro de Recursos Microbiológicos Departamento de Ciências da VidaFaculdade de Ciências e TecnologiaUniversidade Nova de Lisboa2829-516 CaparicaPortugal

Members of the Botryosphaeriaceae are well known fungi associated with dieback,canker and fruit rot on various hosts worldwide,including *** aim of this study was identify a large collection of Botryosphaeriaceae species associated with dieback and stem-end rot of mango in the semi-arid region of Northeastern Brazil,and compare the species in relation to mycelial growth,pathogenicity and virulence.A total of 115 isolates were sampled and based on morphology and DNA sequence data(ITS and EF1-α)seven taxa were identified,namely,Botryosphaeria dothidea,***,Fusicoccum fabicercianum,Neofusicoccum parvum,*** ***,Neoscytalidium dimidiatum and Pseudofusicoccum *** and *** were the most commonly isolated species,which represented 37%and 33%of all isolates *** is reported for the first time in association with mango diseases *** were significant differences among the species obtained in this study in relation to optimum temperature for mycelial growth and mycelial growth *** species were pathogenic on mango *** were significant differences in virulence among the species,with *** and *** being the most virulent species,while *** was the least virulent.

关键词： Botryosphaeriaceae Dieback Stem-end rot Mangifera indica Pathogenicity Virulence Phylogeny

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Defining a species in fungal plant pathology:beyond the species level

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Fungal Diversity 2021年第4期109卷 267-282页

作者： Ishara S.Manawasinghe Alan J.l.phillips Jianping Xu Abhaya Balasuriya Kevin D.Hyde Łukasz Stępień Dulanjalee l.Harischandra Anuruddha Karunarathna Jiye Yan Janith Weerasinghe Mei luo Zhangyong Dong Ratchadawan Cheewangkoon Innovative Institute for Plant Health Zhongkai University of Agriculture and EngineeringGuangzhou 510225People’s Republic of China Faculdade de Ciências Biosystems and Integrative Sciences Institute(BioISI)Universidade de LisboaCampo Grande1749-016 LisbonPortugal Department of Biology McMaster University1280 Main St.WestHamiltonON L8S 4K1Canada Department of Plant Science Faculty of AgricultureRajarata University of Sri LankaAnuradhapuraSri Lanka Center of Excellence in Fungal Research Mae Fah Luang UniversityChiang RaiThailand Institute of Plant Genetics Polish Academy of SciencesPoznańPoland Institute of Plant and Environment Protection Beijing Academy of Agriculture and Forestry SciencesBeijing 100097China Department of Entomology and Plant Pathology Faculty of AgricultureChiang Mai University50200Chiang MaiThailand School of Chemistry and Physics Queensland University of Technology2George StreetBrisbaneQLD 4000Australia

In plant pathology,the correct naming of a species is essential for determining the causal agents of *** names not only serve the general purpose of concise communication,but also are critical for effective plant quarantine,prevent-ing the introduction of new pathogens into a *** phytopathogenic genera have multiple species and,in several genera,disagreements between the multiple prevailing species concept definitions result in numerous cryptic *** of these species were previously called by various names;forma speciales(specialised forms),subspecies,or ***,based on new molecular evidence they are being assigned into new *** frequent name changes and lack of consistent criteria to delineate cryptic species,species,subspecies,forms,and races create increasing confusion,often making communication among biologists ***,such ambiguous information can convey misleading evo-lutionary concepts and species *** aim of this paper is to review these concepts,clarify their use,and evaluate them by referring to existing *** specifically address the question,“Do plant pathogens require a different ranking system?”We conclude that it is necessary to identify phytopathogens to species level based on data from multiple ***,this identification must go beyond species level to clearly classify hitherto known subspecies,forms and *** addition,when naming phytopathogenic genera,plant pathologists should provide more information about geographic locations and host ranges as well as host specificities for individual species,cryptic species,forms or *** describing a new phytopathogen,we suggest that authors provide at least three representative strains together with pathogenicity test *** Koch’s postulates cannot be fulfilled,it is necessary to provide complementary data such as associated disease severity on the host ***,more sequenced collections of spec

关键词： Cryptic species Forms Fungal nomenclature New pathogen Species concepts Subspecies Races

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Substrate Selectivity of Glycerol-3-phosphate Acyl Transferase in Rice

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Journal of Integrative Plant Biology 2009年第11期51卷 1040-1049页

作者： Su-Qin Zhu Hua Zhao Rong Zhou ben-Hua Ji Xiao-Yan Dan School of life Sciences Nantong University Nantong 226007 China

Substrate selectivity of glycerol-3-phosphate acyltransferase （EC 2. 3. 1. 15） of rice （Oryza sativa l.） was explored in a comparative study of acyltransferases from seven plant species. In vitro labeling of acyl carrier protein （ACP） with ^14C or 3H showed that acyltransferase from chill-sensitive plants, such as rice that uses either oleic （18：1） or palmitic acid （16：0） as acyl donor at comparable rates, displays lower selectivity than the enzyme from chill-resistant plants, such as spinach, which preferentially uses oleic acid （18：1） rather than palmitic acid （16：0） as an acyl donor. This may be a result of the size and character of the substrate-binding pocket of acyltransferase. Homology modeling and protein structure-based sequence alignment of acyltransferases revealed that proteins from either chill-sensitive or chill-tolerant plants shared a highly conserved domain containing the proposed substrate-binding pocket. However, the aligned residues surrounding the substrate-binding pocket are highly heterogeneous and may have an influence mainly on the size of the substrate binding pockets of acyltransferases. The substrate selectivity of acyltransferase of rice can be improved by enlarging the substrate-binding pocket using molecular biological methods.

关键词： glycerol-3-phosphate acyt transferase homology modeling rice substrate selectivity.

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Comparison of slow freezing and vitrification methods for Venda cockerel’s spermatozoa

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Open Journal of Animal Sciences 2012年第3期2卷 204-210页

作者： Masindi l. Mphaphathi Dibungi luseba ben Sutherland Tshimangadzo l. Nedambale Agricultural Research Council Animal Production Institute Germplasm Conservation & Reproductive Biotechnologies Irene RSA Tshwane University of Technology Faculty of Science Department of Animal Sciences Pretoria RSA University of the Free State Department of Animal Wildlife and Grassland Sciences Bloemfontein RSA

An improvement in avian semen cryopreservation is essential and has the potential to improve the cryo-gene banking efficiency. This study compared two cryopreservation methods (slow freezing and vitrification) and the effect of different thawing/warming temperatures (5℃, 25℃ and 41℃) on Venda cockerel’s spermatozoa. Semen samples from Venda cockerels were diluted with modified Kobidil+ extender supplemented with 8% dimethyl sulfoxide. Semen from each ejaculate was stained with nigrosin/eosin for viability examination. The cryopreserved samples were either slow cooled in 0.25 ml straw or vitrified in a solid surface vitrification (SSV) device. Semen straw or cryovial was stored in liquid nitrogen container. The straw or cryovial with sperm was thawed or warmed at 5?C, 25?C and 41℃ and analysed by a Computer-Aided Sperm Analysis (CASA). There was a significant difference in live/normal sperm between the semen donors. Cockerels spermatozoa cryopreserved by slow freezing (43%) and thawed at 5?C had a significantly higher survival and motility rate compared to vitrification (2.5%) method. In conclusion, there was higher rate of live/normal morphology sperm. Cryopreservation process reduces sperm motility and velocity rate regardless of cryoprevervation method and thawing or warming temperatures. However, slow freezing was a better method to maintain motility of spermatozoa following cryopreservation.

关键词： Venda Cockerels Dimethyl Sulfoxide Sperm Motility Cryopreservation

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MPPT Design Using PSO Technique for Photovoltaic System Control Comparing to Fuzzy logic and P&O Controllers

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Energy and Power Engineering 2016年第11期8卷 349-366页

作者： O. ben Belghith l. Sbita F. Bettaher Research Unit of Photovoltaic Wind and Geothermal Systems National Engineering School of Gabes (ENIG) University of Gabes Rue Omar Ibn-Elkhattab Zrig Gabes Tunisia Hatem Bettaher Research Unit of Computing Networks Communication Systems and Mathematics IResCoMath Higher Institute of In-formatics and Multimedia of Gabes (ISIMG) University of Gabes Gabes Tunisia

The Maximum Power Point Tracker (MPPT) is the optimum operating point of a photovoltaic module. It plays a very important role to obtain the maximum power of a solar panel as it allows an optimal use of a photovoltaic system, regardless of irradiation and temperature variations. In this research, we present a novel technique to improve the control’s performances optimization of the system consisting of a photovoltaic panel, a buck converter and a load. Simulations of different parts of the system are developed under Matlab/Simulink, thus allowing a comparison between the performances of the three studied controllers: “Fuzzy TS”, “P&O” and “PSO”. The three algorithms of MPPT associated with these techniques are tested in different meteorological conditions. The obtained results, in different operating conditions, reveal a clear improvement of controlling performances of MPPT of a photovoltaic system when the PSO tracking technique is used.

关键词： Photovoltaic System MPPT Controller Buck Converter Perturb and Observe “P&O” Fuzzy logic “Fuzzy TS” Particle Swarm Optimization “PSO”

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澳大利亚南部植物群落中的兰花的结实成功率与周围花卉资源无关

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Journal of Plant Ecology 2023年第2期16卷 204-212页

作者： Irene Martín-Forés Samantha l.Bywaters ben Sparrow Greg R.Guerin Department of Ecology and Evolutionary Biology School of Biological SciencesThe University of AdelaideAdelaideSA 5005Australia Terrestrial Ecosystem Research Network(TERN) The University of AdelaideAdelaideSA 5005Australia

为保证生态系统不断补充兰花的种群数量,了解兰花周围的花卉资源如何影响兰花的结实成功率是很有必要的。为此,本论文研究了澳大利亚的两种特有物种Diuris pardina和Glossodia major的结实成功率与其周围花卉资源之间的关系。***模仿豌... 详细信息

为保证生态系统不断补充兰花的种群数量,了解兰花周围的花卉资源如何影响兰花的结实成功率是很有必要的。为此,本论文研究了澳大利亚的两种特有物种Diuris pardina和Glossodia major的结实成功率与其周围花卉资源之间的关系。***模仿豌豆花,通过视觉欺骗手段吸引Pultenaea共花植物的专化传粉者;***展现假花药,吸引泛化传粉者。因为吸引密度更高的本地蜜蜂可以促进兰花传粉,我们假设这两个物种的结实成功率与其周围同种花和异种花的密度呈正相关。我们还假设***的成功结实与其周围Pultenaea花的丰度呈正相关。我们调查了澳大利亚南部的18个地块,统计了同种花和异种花的物种数、个体数和花朵数,以及成功结实的花朵数。对结实成功率与同种花的密度、周围花资源的多度、丰度和香农指数以及单个物种的花卉丰度进行了Pearson相关分析,发现***的结实成功率与周围同种花的密度相关,但***的结实成功率与周围同种花的密度无关,未发现结实成功率与异种花资源之间存在关联。***的结实成功率与Pultenaea的丰度无关,而与入侵物种法国薰衣草(lavandula stoechas)呈正相关。

关键词：澳大利亚欺骗性的特有兰花花资源结实成功授粉

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