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Neuronal-like cell differentiation of non-adherent bone marrow cell-derived mesenchymal stem cells

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Neural Regeneration Research 2013年第22期8卷 2078-2085页

作者： Yuxin Wu Jinghan Zhang Xiaoming ben Department of Neurosurgery Nanjing Children's Hospital of Nanjing Medical University Department of Newborn Medicine Nanjing Children's Hospital of Nanjing Medical University Department of Newborn Medicine Shanghai First Maternity and Infant HospitalTongji University School of Medicine

Non-adherent bone marrow cell-derived mesenchymal stem cells from c57BL/6J mice were sepa- rated and cultured using the ＂pour-off＂ method. Non-adherent bone marrow cell-derived mesen- chymal stem ceils developed colony-forming unit-fibroblasts, and could be expanded by supple- mentation with epidermal growth factor. Immunocytochemistry showed that the non-adherent bone marrow cell-derived mesenchymal stem cells exposed to basic fibroblast growth factor/epidermal growth factor/nerve growth factor expressed the neuron specific markers, neurofilament-200 and NeuN, in vitro. Non-adherent bone marrow cell-derived mesenchymal stem cells from 13-galactosidase transgenic mice were also transplanted into focal ischemic brain （right corpus striatum） of c57BL/6J mice. At 8 weeks, cells positive for LacZ and 13-galactosidase staining were observed in the ischemic tissues, and cells co-labeled with both 13-galactosidase and NeuN were seen by double immunohistochemical staining. These findings suggest that the non-adherent bone marrow cell-derived mesenchymal stem cells could differentiate into neuronal-like cells in vitro and in vivo.

关键词： neural regeneration stem cells non-adherent bone marrow cell-derived mesenchymal stem cells neuronal-like cells colony-forming unit-fibroblasts proliferation differentiation beta-galactosidasetransgenic mouse cell transplantation cerebral ischemia bone marrow cells-derived mesenchymalstem cells grants-supported paper neuroregeneration

新凝血四项与急性髓系白血病患者红细胞输注治疗期间肾功能的相关性研究

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临床血液学杂志 2024年第2期37卷 107-112页

作者：牛奔侯丽敏李岚陕西省人民医院血液科西安710068

目的:分析新凝血四项与急性髓系白血病(acute myeloid leukemia, AML)患者红细胞输注治疗期间肾功能的关系。方法:选择2019年6月至2022年5月陕西省人民医院血液科收入的96例AML患者作为研究对象,均接受红细胞输注治疗,以治疗期间发生急... 详细信息

目的:分析新凝血四项与急性髓系白血病(acute myeloid leukemia, AML)患者红细胞输注治疗期间肾功能的关系。方法:选择2019年6月至2022年5月陕西省人民医院血液科收入的96例AML患者作为研究对象,均接受红细胞输注治疗,以治疗期间发生急性肾损伤(acute kidney injury, AKI)为终点事件。入科后即采血检测患者血常规、凝血四项、新凝血四项、肾功能及一般资料,根据检查结果,分析新凝血四项与AML患者红细胞输注治疗期间发生AKI的关系。结果:研究入选AML患者96例,红细胞输注无效4例,Ⅳ级骨髓抑制2例,最终90例患者作为最终研究对象。90例患者红细胞输注时间为13.00(11.75,14.00)周,22例(24.44%)患者在红细胞输注治疗期间发生AKI。AKI患者红细胞输注次数、红细胞输注量高于N-AKI患者,血浆血栓调节蛋白(TM)、组织型纤溶酶原激活物-抑制剂-1复合物(t-PAIc)、血浆胱抑素c(cysc)表达高于N-AKI患者,血浆凝血酶-抗凝血酶复合物(TAT)、纤溶酶a2纤溶酶抑制剂复合物(PIc)表达低于N-AKI患者,差异有统计学意义(Pc回归分析发现,红细胞输注次数、血浆TM、t-PAIc表达上调是AML患者红细胞输注治疗期间发生AKI的独立因素(Pc曲线,新凝血四项中血浆TM、t-PAIc均能够预测AML患者红细胞输注治疗期间AKI发生。结论:新凝血四项中血浆TM、t-PAIc表达上调是AML患者红细胞输注治疗期间发生AKI的危险因素,红细胞输注治疗前检测血浆TM、t-PAIc有助于预测AKI发生。

关键词：急性髓系白血病红细胞输注治疗急性肾损伤凝血功能

Epo/EpoR signaling in osteoprogenitor cells is essential for bone homeostasis and Epo-induced bone loss

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Bone Research 2021年第4期9卷 549-556页

作者： Martina Rauner Marta Murray Sylvia Thiele Deepika Watts Drorit Neumann Yankel Gabet Lorenz c.Hofbauer ben Wielockx Department of Medicine III&center for Healthy Aging Technische Universitat DresdenDresdenGermany lnstitute for clinical chemistry and Laboratory Medicine Technische Universitat DresdenDresdenGermany Department of cell and Developmental Biology Sackler Faculty of MedicineTel Aviv UniversityTel AvivIsrael Department of Anatomy&Anthropology Sackler Faculty of MedicineTel Aviv UniversityTel AvivIsrael

High erythropoietin(Epo)levels are detrimental to bone health in adult *** mice receiving high doses of Epo lose bone mass due to suppressed bone formation and increased bone *** humans,high serum Epo levels are linked to fractures in elderly *** earlier studies indicated that Epo modulates osteoblast activity;however,direct evidence that Epo acts via its receptor(EpoR)on osteoblasts in vivo is still ***,we created mice lacking EpoR in osteoprogenitor cells to specifically address this *** of EpoR in osteoprogenitors(EpoR:Osx-cre,cKO)starting at 5 weeks of age did not alter red blood cell parameters but increased vertebral bone volume by 25%in 12-week-old female *** was associated with low bone ***(osteoblast number,bone formation rate)and serum(P1NP,osteocalcin)bone formation parameters were all reduced,as were the number of osteoclasts and TRAP serum *** of osteoblast precursors isolated from cKO versus control mice resulted in lower expression of osteoblast marker genes including Runx2,Alp,and col1a1 on day 21,whereas the mineralization capacity was ***,the RANKL70PG ratio,which determines the osteoclast-supporting potential of osteoblasts,was substantially decreased by 50%.Similarly,coculturing cKO osteoblasts with control or cKO osteoclast precursors produced significantly fewer osteoclasts than coculture with control ***,exposing female mice to Epo pumps(10 U-d_1)for 4 weeks resulted in trabecular bone loss(-25%)and increased osteoclast numbers(1.7-fold)in control mice only,not in cKO *** data show that EpoR in osteoprogenitors is essential in regulating osteoblast function and osteoblast-mediated osteoclastogenesis via the RANKI70PG ***,osteogenic Epo/EpoR signaling controls bone mass maintenance and contributes to Epo-induced bone loss.

关键词： progenitor homeostasis doses

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不同改性生物炭对稻田氮素损失及产量的影响

中国环境科学 2024年第9期44卷 5122-5133页

作者：衣本骥刘畅韩宏伟张丰陈涛涛孟军迟道才沈阳农业大学水利学院辽宁沈阳110866 沈阳农业大学国家生物炭研究院农业农村部生物炭与土壤改良重点实验室辽宁沈阳110866

为进一步挖掘稻田生态系统生物炭增效、节肥和减排潜力,以无生物炭为对照(cK),设置玉米秸秆生物炭(B)、酸化生物炭(B_(pH))、氮负载生物炭(B_(NH_(4)))、铁改性生物炭(B_(Fe))和钙改性生物炭(B_(ca))6个处理,通过2a试验,对生物炭阳离子... 详细信息

为进一步挖掘稻田生态系统生物炭增效、节肥和减排潜力,以无生物炭为对照(cK),设置玉米秸秆生物炭(B)、酸化生物炭(B_(pH))、氮负载生物炭(B_(NH_(4)))、铁改性生物炭(B_(Fe))和钙改性生物炭(B_(ca))6个处理,通过2a试验,对生物炭阳离子交换量(cEc)、含氧官能团以及其处理下水稻产量、氮素提取、氨挥发、氮素淋失和土壤氮残余等进行了研究.结果表明:与B相比,B_(pH)、B_(NH_(4))和B_(Fe)分别显著提升生物炭cEc(53.9%、29.6%和28.2%),且4种改性处理均可显著提升生物炭-cOOH、-OH、c=O、O-H等含氧官能团浓度,增强生物炭吸氮能力.全生育期累积氨挥发在9.1~19.9kg/hm^(2)(2021)和8.5~13.7kg/hm^(2)(2022)之间,除B_(NH_(4))降氨效果明显(44.6%)外,其他处理第1年对氨挥发无显著影响;而B_(ca)、B_(NH_(4))和B_(Fe)在第2a均显著降低氨挥发,较cK分别降低33.5%、37.5%和37.8%.4种改性生物炭连续2a,较cK降低氮素淋失19.0%和35.2%(B_(pH))、15.0%和21.0%(B_(ca))、11.3%和28.7%(B_(NH_(4)))、12.6%和29.0%(B_(Fe)).B在第1年对产量无显著影响,但老化1年后增产显著(8.8%);B_(pH)和B_(NH_(4))连续2a分别增产19.7%和12.2%(2021年),11.5%和5.8%(2022年).B_(pH)、B_(ca)和B_(NH_(4))在2a均显著降低了活性氮排放,降幅为23.4%、21.8%和42.3%(2021年)和23.5%、30.5%和35.5%(2022年).不同生物炭处理第1a土壤矿化氮残余无显著差异;但B_(ca)、B_(NH_(4))和B_(Fe)第2a增效显著.同时考虑增产、活性氮减排和矿化氮可持续性,B_(NH_(4))综合效果最佳,同cK相比,2021和2022年分别可降低氨挥发42.3%和35.5%,铵态氮淋失10.6%和29.0%,硝态氮淋失14.7%和23.4%,氮素积累提高13.4%和4.3%以及矿化氮残余提高15.0%和17.9%,最终增产19.7%和5.7%.

关键词：不同改性生物炭田面水氨挥发淋溶水稻产量氮素去向

Spin-dependent transport properties and Seebeck effects for a crossed graphene superlattice p-n junction with armchair edge

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Frontiers of physics 2018年第4期13卷 145-157页

作者： ben-Hu Zhou ben-Liang Zhou Yang-Su Zeng Man-Yi Duan Guang-Hui Zhou Department of Physics Shaoyang University Shaoyang 422001 China Department of Physics and Key Laboratory for Low-Dimensional Structures and Quantum Manipulation (Ministry of Education) Hunan Normal University Changsha 410081 China college of Physics and Electronic Engineering Sichuan Normal University Chengdu 610068 China

Using the nonequilibrium Green＇s function method combined with the tight-binding Hamiltonian, we theoretically investigate the spin-dependent transmission probability and spin Seebeck coefficient of a crossed armchair-edge graphene nanoribbon （AGNR） superl＇attice p-n junction under a perpendicular magnetic field with a ferromagnetic insulator, where junction widths Wi of 40 and 41 are considered to exemplify the effect of semiconducting and metallic AGNRs, respectively. A pristine AGNR system is metallic when the transverse layer m = 3j ＋ 2 with a positive integer j and an insulator otherwise. When stubs are present, a semiconducting AGNR junction with width W1= 40 always shows metallic behavior regardless of the potential drop magnitude, magnetization strength, stub length, and per- pendicular magnetic field strength. However, metallic or semiconducting behavior can be obtained from a metallic AGNR junction with Wi = 41 by adjusting these physical parameters. Furthermore, a metal-to-semiconductor transition can be obtained for both superlattice p-n junctions by adjust- ing the number of periods of the superlattice. In addition, the spin-dependent Seebeck coefficient and spin Seebeck coefficient of the two systems are of the same order of magnitude owing to the appearance of a transmission gap, and the maximum absolute value of the spin Seebeck coefficient reaches 370 μV/K when the optimized parameters are used. The calculated results offer new possi- bilities for designing electronic or heat-spintronic nanodevices based on the graphene superlattice p-n junction.

关键词： crossed graphene superlattice p-n junction spin-dependent transport properties Seebeck coefficient nonequilibrium Green's function

AVirtual cloud Storage Architecture for Enhanced Data Security

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computer Systems Science & Engineering 2023年第2期44卷 1735-1747页

作者： M.Antony Joans Kumar c.christopher columbus E.ben George T.Ajith Bosco Raj Department of Information Technology St.Xavier’s Catholic College of EngineeringNagercoil629003Tamil NaduIndia Department of computer Science Engineering PSN College of Engineering and TechnologyTirunelveli627152India Department of computer Science Engineering University of Technology and Applied SciencesMuscat112Oman Department of Electronics and communications Engineering PSN College of Engineering and TechnologyTirunelveli627152India

The sensitive data stored in the public cloud by privileged users,such as corporate companies and government agencies are highly vulnerable in the hands of cloud providers and *** proposed Virtual cloud Storage Archi-tecture is primarily concerned with data integrity and conﬁdentiality,as well as *** provide conﬁdentiality and availability,theﬁle to be stored in cloud storage should be encrypted using an auto-generated key and then encoded into distinct *** the encoded chunks ensured theﬁle integrity,and a newly proposed circular Shift chunk Allocation technique was used to determine the order of chunk ***ﬁle could be retrieved by performing the opera-tions in *** the regenerating code,the model could regenerate the missing and corrupted chunks from the *** proposed architecture adds an extra layer of security while maintaining a reasonable response time and sto-rage *** results analysis show that the proposed model has been tested with storage space and response time for storage and *** VcSA model consumes 1.5x(150%)storage *** was found that total storage required for the VcSA model is very low when compared with 2x Replication and completely satisﬁes the cIA *** response time VcSA model was tested with different sizedﬁles starting from 2 to 16 *** response time for storing and retrieving a 2 MBﬁle is 4.96 and 3.77 s respectively,and for a 16 MBﬁle,the response times are 11.06 s for storage and 5.6 s for retrieval.

关键词： Erasure code regenerating code cryptography virtual cloud storage architecture circular shift chunk allocation algorithm

Effects of radiation-induced crosslinking on thermal and mechanical properties of poly（lactic acid） composites reinforced by basalt fiber

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Nuclear Science and Techniques 2013年第B12期24卷 73-78页

作者： LIU Meihua YIN Yuan WEI Wei ZHENG chunbai SHEN shirley DENG Pengyang ZHANG Wanxi Department of Materials Science and Engineering Jilin University Jilin 130025 China changchun Institute of Applied chemistry Chinese Academy of Science Jilin 130022 China cSIRO Materials Science and Engineering 37 Graham Road Highett VIC 3190 Australia

Poly(lactic acid)/ basalt fiber (PLA/BF) composites were prepared by melt blending with a cross-linking agent, triallyl isocyanurate (TAIc). The thermal and mechanical properties of the composites were investigated through gel fraction, heat defection temperature (HDT), tensile tests and scanning electron microscopy (SEM). Under certain conditions, the HDT of composites was dramatically increased to 140°c after irradiation. Tensile properties were enhanced as well. Both these improvements were consistent with changes of the fracture morphology. compatibilization and concomitant enhancement of the interfacial adhesive between the polymer matrix and the inorganic fiber were achieved as seen from SEM photos, as a result of the formation of co-crosslinking and grafting structures at the interface according to the determination of gelation extraction. © 2013, Science Press. All rights reserved.

关键词：玄武岩纤维复合材料共交联剂机械性能热性能辐射诱导乳酸三烯丙基异氰脲酸酯

Three-dimensional modelling of shear keys in concrete gravity dams using an advanced grillage method

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Water Science and Engineering 2020年第3期13卷 223-232页

作者： Mahdi ben Ftima Steephane Lafrance Pierre Leeger Polytechnique Montreal Montreal University CampusMontrealH3C 3A7Canada

contraction joint shear keys are resilient features of gravity dams that can be considered to increase the sliding safety factors or minimise seismic residual sliding displacements,allowing costly remedial actions to be *** paper presents a novel,robust,and computationally efficient three-dimensional(3D)modelling and simulation strategy of gravity dams,using a series of adjacent cantilever beam elements to represent individual *** monoliths are interconnected in the longitudinal direction by 3D no-tension link elements representing the lumped shear key stiffness contributions at a particular *** objective is to assess the shear key internal force demands,including the axial force,shear,and moment *** key demand-capacity ratios can then be assessed with related multi-axial failure *** 3D link element stiffness coefficients were derived from a series of 3D finite element(FE)solid models with a detailed representation of geometrical features of multiple shear *** results from the proposed method based on advanced grillage analysis show strong agreement with reference solutions from 3D FE solid models,demonstrating high accuracy and performance of the proposed *** application of the proposed advanced grillage method to a dam model with two monoliths clearly shows the advantage of the proposed method,in comparison to the classical approach used in practise.

关键词： Gravity dam Shear keys Seismic analysis Grillage analysis Finite element model Multi-scale approach

P2X7 receptor signaling during adult hippocampal neurogenesis

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Neural Regeneration Research 2019年第10期14卷 1684-1694页

作者： Hannah c. Leeson Tailoi chan-Ling Michael D. Lovelace Jeremy c. Brownlie ben J. Gu Michael W. Weible II Australian Institute for Bioengineering and Nanotechnology The University of QueenslandBrisbaneQueenslandAustralia Griffith Institute for Drug Discovery Griffith UniversityBrisbaneQueenslandAustralia Discipline of Anatomy and Histology School of Medical ScienceThe University of SydneySydneyNew South WalesAustralia Bosch Institute The University of SydneySydneyNew South WalesAustralia Applied Neurosciences Program Peter Duncan Neurosciences Research UnitSt. Vincent’s Centre for Applied Medical ResearchSydneyNew South WalesAustralia Faculty of Medicine St. Vincent’s Clinical SchoolUniversity of New South Wales SydneySydneyNew South WalesAustralia School of Environment and Science Griffith UniversityBrisbaneQueenslandAustralia Florey Institute of Neuroscience and Mental Health University of MelbourneMelbourneVictoriaAustralia

Neurogenesis is a persistent and essential feature of the adult mammalian *** neurons generated from resident pools of stem or progenitor cells provide a mechanism for the formation and consolidation of new *** of hippocampal neurogenesis is complex and multifaceted,and numerous signaling pathways converge to modulate cell proliferation,apoptosis,and clearance of cellular debris,as well as synaptic integration of newborn immature *** expression of functional P2X7 receptors in the central nervous system has attracted much interest and the regulatory role of this purinergic receptor during adult neurogenesis has only recently begun to be explored.P2X7 receptors are exceptionally versatile:in their canonical role they act as adenosine triphosphate-gated calcium channels and facilitate calcium-signaling cascades exerting control over the cell via calcium-encoded sensory proteins and transcription factor activation.P2X7 also mediates transmembrane pore formation to regulate cytokine release and facilitate extracellular communication,and when persistently stimulated by high extracellular adenosine triphosphate levels large P2X7 pores form,which induce apoptotic cell death through cytosolic ion ***,as a scavenger receptor P2X7 directly facilitates phagocytosis of the cellular debris that arises during neurogenesis,as well as during some disease *** how P2X7 receptors regulate the physiology of stem and progenitor cells in the adult hippocampus is an important step towards developing useful therapeutic models for regenerative *** review considers the relevant aspects of adult hippocampal neurogenesis and explores how P2X7 receptor activity may influence the molecular physiology of the hippocampus,and neural stem and progenitor cells.

关键词： P2X7 P2X7R adult neurogenesis neural stem cells neural progenitor cells hippocampus SGZ calcium signaling purinergic signaling