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Fatty acid hydratase for value-added biotransformation:A review

Chinese Journal of Chemical engineering 2020年第8期28卷 2051-2063页

作者： Yan Zhang bekir engin eser Peter Kristensen Zheng Guo Department of engineering Faculty of Science and TechnologyAarhus UniversityGustav wieds vej 108000 AarhusDenmark Faculty of engineering and Science Department of Chemistry and BioscienceAalborg University9220 AalborgDenmark

The synthesis of hydroxy fatty acids(HFAs) from renewable oil feedstock by addition of water onto C_C bonds has attracted great attention in recent years. Given that selective asymmetric hydration of non-activated C_C bonds has been proven difficult to achieve with chemical catalysts, enzymatic catalysis by fatty acid hydratases(FAHs) presents an attractive alternative approach to produce value-added HFAs with high regio-, enantioand stereospecificity, as well as excellent atom economy. Even though FAHs have just been investigated as a potential biocatalyst for a decade, remarkable information about FAHs in different aspects is available;however, a comprehensive review has not been archived. Herein, we summarize the research progresses on biochemical characterization, structural and mechanistic determination, enzyme engineering, as well as biotechnological application of FAHs. The current challenges and opportunities for an efficient utilization of FAHs in organic synthesis and industrial applications are critically discussed.

关键词： Hydroxy fatty acids Fatty acid hydratases Enzyme characterization Catalytic mechanism Enzyme engineering Biotransformation

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Self-sufficient Cytochrome P450s and their potential applications in biotechnology

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Chinese Journal of Chemical engineering 2021年第2期34卷 121-135页

作者： bekir engin eser Yan Zhang Li Zong Zheng Guo Department of engineering Faculty of Technical SciencesAarhus UniversityDenmark Key Laboratory for Molecular Enzymology and engineering The Ministry of EducationSchool of Life ScienceJilin UniversityChangchun 130021China

Cytochrome P450s(CYPs)are ubiquitously found in all kingdoms of life,playing important role in various biosynthetic pathways as well as degradative pathways;accordingly find applications in a vast variety of areas from organic synthesis and drug metabolite production to modification of biomaterials and ***,CYPs catalyze chemically challenging CAH and CAC activation reactions using a reactive high-valent iron-oxo intermediate generated upon dioxygen activation at their heme center,while the other oxygen atom is reduced to the level of water by electrons provided through a reductase partner ***-sufficient CYPs,encoding their heme domain and reductase protein in a single polypeptide,facilitate increased catalytic efficiency and render a less complicated system to work *** self-sufficient CYP enzyme from CYP102A family(CYP102A1,BM3)is among the earliest and most-investigated model enzymes for mechanistic and structural studies as well as for biotechnological *** increasing number of self-sufficient CYPs from the same CYP102 family and from other families have also been reported in last *** this review,we introduce chemistry and biology of CYPs,followed by an overview of the characteristics of self-sufficient CYPs and representative *** engineering efforts leading to novel self-sufficient CYP variants that can catalyze synthetically useful natural and non-natural(nature-mimicking)reactions are ***,the strategy and efforts that aim to circumvent the challenges for improved thermostability,regio-and enantioselectivity,and total turnover number;associated with practical use of self-sufficient CYPs are reviewed.

关键词： Biocatalysis Heme enzymes CAH activation Cytochrome P450s Self-sufficient P450s P450 BM3

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