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Glutamine synthetase as an early marker for hepatocellular carcinoma based on proteomic analysis of resected smal hepatocel ular carcinomas

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Hepatobiliary & Pancreatic Diseases International 2010年第3期9卷 296-305页

作者： Jiang Long,Zhen-Wei Lang,Hua-Guang Wang,Tai-Ling Wang,Bao-En Wang and Si-Qi Liu Liver Research Center,beijing friendship hospital, Capital Medical University,beijing 100050,china Department of Hepatology,Department of Pathology, beijing Youan hospital,Capital Medical University,beijing 100069,china Department of Pharmaceutical Affairs,beijing Chaoyang hospital,Capital Medical University,beijing 100020,china Department of Pathology,china-Japan friendship hospital,beijing 100029,china beijing Genomics Institute,Chinese Academy of Science,beijing Airport Industrial Zone B-6,Shunyi,beijing 101300,china Liver Research Center Beijing Friendship Hospital Capital Medical University Beijing China.

BACKGROUND:Hepatocellular carcinoma(HCC)is a highly malignant tumor with a poor *** small HCCs possess most of the characteristics of early HCC,we investigated small HCCs to screen potential biomarkers for early ***:Proteins were extracted from 10 sets of paired tissue samples from HBV-infected small-HCC *** extracted proteins were well resolved by two-dimensional *** HCC-associated proteins were then identified by MALDI-TOF/TOF MS following image *** blotting and immunohistochemistry were used to assess glutamine synthetase(GS)and phenazine biosynthesislike domain-containing protein(PBLD)expression in liver ***-linked immunosorbent assays in 152 serum samples(from 49 healthy donors,24 patients with liver cirrhosis,and 79 with HCC)were used to further assess the significance of GS ***:Fifteen up-regulated and three down-regulated proteins were *** blotting confirmed GS overexpression and decreased PBLD expression in liver *** showed that GS was expressed in 70.0%(84/120)of HCCs and 35.8%(43/120)of nontumor tissues;PBLD was expressed in 74.2%(89/120) of nontumor tissues and 40.8%(49/120)of *** Chi-square test showed significant expression differences between HCCs and adjacent *** with this,serum GS levels in HCC patients were significantly higher than those in liver cirrhosis patients and healthy donors,while the latter two groups were also significantly *** addition, a diagnostic cutoff value of 2.6 mg/ml was used for GS;it was elevated in 19(76.0%)of 25 HCC patients with AFP≤20 ng/ml and 47(88.7%)of 53 HCC patients with AFP≤200 ng/***:GS and PBLD are abnormally expressed in most *** may be a novel serum marker for early HCC, especially for those patients with low AFP levels(≤200 ng/ml).

关键词： glutamine synthetase phenazine biosynthesis-like domain-containing protein proteomics

Antiviral therapy with nucleos(t)ide analogues for severe chronic hepatitis B

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Hepatobiliary & Pancreatic Diseases International 2009年第4期8卷 438-439页

作者： Fu-Kui Zhang, Liver Research Center, beijing friendship hospital, Capital Medical University, beijing 100050,china Capital Med Univ Beijing Friendship Hosp Liver Res Ctr Beijing 100050 Peoples R China

To the Editor:I read the paper by Chen et al[1]with great *** authors performed a retrospective study to evaluate the short-term efficacy of antiviral therapy with

关键词： CHB HBV Antiviral therapy with nucleos t)ide analogues for severe chronic hepatitis B

Clinical and endoscopic features of Chinese reflux esophagitis patients

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World Journal of Gastroenterology 2008年第12期14卷 1866-1871页

作者： Wei Li, Shu-Tian Zhang, Zhong-Lin Yu, Department of Gastroenterology, beijing friendship hospital Affiliated to Capital Medical University Faculty of Gastroenterology, Capital Medical University beijing Digestive Disease Center, beijing 100050, china Department of Gastroenterology Beijing Friendship Hospital Affiliated to Capital Medical University Beijing Digestive Disease Center Beijing 100050 China.

AIM： To analyze the clinical and endoscopic features of Chinese patients with reflux esophagitis （RE）. METHODS： A total of 1405 RE patients were analyzed retrospectively. Data on gender, age, presence/absence of H pylori infection and associated esophageal hiatal hernia were collected. Esophagitis was divided into different grades according to Los Angeles Classification. RESULTS： Of 18823 patients, 1405 were diagnosed as RE. The ratio of male to female patients was 1.75：1 （P 〈 0.01）. The mean age of male and female patients was significantly different （P = 0.01）. The peak age at onset of the disease was 40-60 years. According to Los Angeles Classification, there were significant differences in the age of patients with grades A and B compared to patients with grades C and D （P 〈 0.01）. Two hundred and seventy-seven patients were infected with H pyiorl, the infection rate was low （P 〈 0.01）. Complication of esophageal hiatal hernia was found to be significantly associated with the severity of esophagitis and age in 195 patients （P 〈 0.01）. Esophageal mucosa damages were mainly located at the right esophageal wall. CONCLUSION： The peak age of onset of RE is 40-60 years and higher in males than in females. The mean age of onset of RE is lower in males than in females. The infection rate of Hpylori is significantly decreased in patients with esophagitis. Old age and esophageal hiatal hernia are associated with more severe esophagitis. Right esophageal mucosal damage can occur more often in RE patients.

关键词： Esophagitis Los Angeles Classification Helicobacterpylori Hiatal hernia

Clearance of HCV RNA in peripheral blood mononuclear cell as a predictor of response to antiviral therapy in patients with chronic hepatitis C

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Hepatobiliary & Pancreatic Diseases International 2005年第4期4卷 550-553页

作者： Dao-Zhen Xu, Yao Xie and Zheng-Qin Li beijing ditan hospital, beijing 100011, china beijing ditan hospital Beijing 100011 China. xudzh@***

BACKGROUND: The resolution of hepatitis C, evidenced by normalization of liver function and disappearance of hepatitis C virus RNA from serum as determined by conventional laboratory assays, reflects virus eradication. But in interferon treated patients the HCV RNA in serum sometimes could not show the virus in cells. Such factors as virus genotype, HCV RNA contents in serum, HCV specific cellular immunities after treatment were reported to predict the response to interferon therapy. In most patients, HCV RNA could detect the virus in peripheral blood mononucle-ar cell. The aim of this study was to investigate the predictive value of HCV RNA in PBMC of patients with chronic hepatitis C after interferon treatment. METHODS: Sixteen patients with chronic hepatitis C were treated with interferon for 24 weeks, and they all get complete responses at 12 weeks of treatment. At the end of treatment, the HCV RNA in PBMC and serum were detected by RT-PCR, and after stopping treatment, HCV RNA in serum was monitored continually. RESULTS: In 9 patients who were HCV RNA positive in their PBMC at the end of treatment, 8 showed serum HCV RNA positive after 24 weeks and another 1 after 1 year. In 7 patients with negative HCV RNA in their PBMC, only 2 patients relapsed in serum HCV RNA after 1-year follow-up, and others remained viral response after 3.5 years. CONCLUSION: HCV RNA in PBMC at the end of IFN treatment is a predictor of durable response to antiviral therapy in patients with chronic hepatitis C.

关键词： chronic hepatitis C HCV RNA interferon peripheral blood mononuclear cell

Lamivudine treatment of decompensated hepatitis B virus-related cirrhosis

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Hepatobiliary & Pancreatic Diseases International 2006年第1期5卷 10-15页

作者： Fu-Kui Zhang Liver Research Center, beijing friendship hospital, Capital University of Medical Sciences, beijing 100050, china Liver Research Center Beijing Friendship Hospital Capital University of Medical Sciences Beijing 100050 China. frankliver@***

BACKGROUND: Patients with decompensated hepatitis B vires (HBV)-related cirrhosis tend to have low or undetectable HBV replication. However, some patients continue to have high levels of HBV replication and effective suppression of HBV replication with antiviral agents may potentially decrease hepatic necroinflammation and improve or stabilize liver function. This review was to under stand the efficacy and safety of lamivudine in the treatment of decompensated HBV cirrhosis. DATA SOURCES: An English-language literature search (MEDLINE January 1988-July 2005) was performed, and a total of 52 articles/abstracts relevant to the issue were selected. After review of the selected papers, the meaningful results and conclusions were extracted using scientific crite ria. The papers reviewed pertained mainly to the efficacy and safety profiles of lamivudine treatment for decompensated HBV cirrhosis. RESULTS: The ultimate treatment of decompensated HBV cirrhosis is liver transplantation, but lamivudine treatment may lead to rapid suppression of viral replication and improvement of biochemical and clinical parameters, reduced morbidity and hospitalization for complications of liver disease, increased pre-transplant survival as well as reduced need for transplantation. However, viral resistance can develop after prolonged treatment with lamivudine, and breakthrough hepatitis may be fatal in few patients. Adefovir is effective for lamivudine-resistant HBV mutants. CONCLUSIONS: Antiviral therapy with lamivudine for decompensated HBV cirrhosis can be effective. However, some patients may experience a hepatitis flare with the emergence of YMDD mutants resulting in progressive worsening of liver disease, and should be referred for 'rescue' therapy with other nucleoside/nucleotide analogues such as adefovir dipivoxil.

关键词： lamivudine adefovir dipivoxil hepatitis B virus cirrhosis

Progress in studies on the characteristics of human amnion mesenchymal cells

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Progress in Natural Science:Materials International 2009年第9期19卷 1047-1052页

作者： Jingwei Hu a,b,c, Zhe Cai c,*, Zhongshu Zhoub,* a Graduate School of beijing Union Medical College, beijing 100730, china b Pediatrics Department of china–Japan friendship hospital, beijing 100029, china c Institute of Clinical Medical Science, china–Japan friendship hospital, beijing 100029, china Graduate School of beijing Union Medical College Beijing 100730 China Pediatrics Department of china–Japan friendship hospital Beijing 100029 China Institute of Clinical Medical Science China–Japan Friendship Hospital Beijing 100029 China

The amniotic membrane (AM) in humans is the innermost fetal membrane and is composed of amnion mesenchymal cells (hAMCs) and amnion epithelial cells (hAECs). The former are derived from the extra-embryonic mesoderm of the primitive streak, and the latter from the fetal ectoderm. Numerous studies have shown that both hAMCs and hAECs display stem cell characteristics. In this review, we examine the progress made in understanding the characteristics of hAMCs, including information on the structure and function of AM and hAMCs, their immunological features, and the pluripotency of hAMCs.

关键词： Human amnion mesenchymal cells Hypo-immunogenicity Immunosuppression Differentiation

Changes in EEG measurements in intractable epilepsy patients with neurofeedback training

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Progress in Natural Science:Materials International 2009年第11期19卷 1509-1514页

作者： Longlian Zhao~a,Wenqing Wu~b,Zuoqing Liang~a,Guangshu Hu~(a,*) a Department of Biomedical Engineering,Tsinghua University,beijing 100084,china b Department of Neural Internal Medicine,beijing friendship hospital,beijing 100050,china Department of Biomedical Engineering Tsinghua University Beijing 100084 China Department of Neural Internal Medicine Beijing Friendship Hospital Beijing 100050 China

To assess the effects of neurofeedback on brain electrophysiology and to determine how biofeedback works,power spectral density (PSD) and approximate entropy(ApEn) analyses are applied to the EEGs of six patients with intractable epilepsy who received neurofeedback *** sessions of treatment,the EEG sensorimotor rhythm to theta PSD ratio calculated from the C4 electrode site becomes larger than that before the treatment,which is consistent with the biofeedback *** ApEn over 16-channel EEG recordings all increase to different *** increases occur in channels located near the training position(C4).All these results suggest that these EEG measurements are new criteria that can be used to evaluate the effect of neurofeedback.

关键词： Biofeedback Electroencephalogram Epilepsy Power spectral density Approximate entropy

Surgical treatment of portal hypertension

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Hepatobiliary & Pancreatic Diseases International 2002年第2期1卷 211-214页

作者： Yu Wang From the Department of General Surgery, beijing friendship hospital, Capital University of Medical Sciences, beijing 100050, china Department of General Surgery Beijing Friendship Hospital Capital University of Medical Sci. Beijing 100050 China

Portal hypertension is a common disease with high mortality and serious influence on the life quality of patients. At present, shunt and disconnection are commonly used for the treatment of portal hyperten- sion. In recent years, combined procedures of shunt and disconnection have evoked the potential interest of surgeons. Initial experimental studies and clinical observations showed that the combined procedures are ideal for treating portal hypertension. Transju- gular intrahepatic portacaval shunt (TIPS) is a new minimally invasive technique in treating portal hy- pertension. Some surgeons have tried to perform dis- connection under laparoscopy with success. Liver transplantation will be the focus of portal hyperten- sion surgery in the future.

关键词： portal hypertension shunt disconnection transjugular intrahepatic portacaval shunt liver transplantation

Interferon-alfa in the treatment of chronic hepatitis B

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Hepatobiliary & Pancreatic Diseases International 2004年第3期3卷 337-340页

作者： Fu-Kui Zhang Liver Research Center, beijing friendship hospital,Capital University of Medical Sciences, beijing 100050 , china Liver Research Center Beijing Friendship Hospital Capital University of Medical Sciences Beijing 100050 China. frankliver@***

BACKGROUND: Interferon-alfa has been used in the treat-ment of chronic hepatitis B for more than 20 years and hasits own advantages including a definite course of therapy,no production of drug-resistant variants, and sustained effi-cacy. This review was to understand the role of interferon-alfa therapy in chronic hepatitis *** RESOURCES: An English-language literature searchusing Medscape and MEDLINE was performed and a totalof 48 articles on the treatment of chronic hepatitis with in-terferon-alfa or pegylated interferon-alfa were ***: Interferon-alfa therapy was associated with ahigher HBV DNA inhibition rate and HBeAg loss rate com-pared with controls, and it may have long-term beneficialeffects in terms of HBV clearance, reduction of hepatocel-lular carcinoma, and prolongation of survival. Pegylatedinterferon-alfa was more effective than conventional inter-feron-alfa in the treatment of chronic hepatitis B as well aschronic hepatitis C, and was also associated with greater ef-ficacy than conventional interferon in difficult-to-treat ***: Interferon-alfa is still regarded as one ofthe first-line drugs for the treatment of chronic hepatitis *** interferon is a more promising therapy than con-ventional interferon-alfa, especially in patients with refrac-tory chronic hepatitis B.

关键词： chronic hepatitis B interferon pegylated interferon lamivudine