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agricultural Sciences in china 2002年第5期1卷 531-534页

作者： LI Zhao-hui, GUO Xing-qi, YE Bao-hua and GUO Yan-kui( college of plant protection , shandong agricultural university, taian 271018 , P.R.china college of Life Sciences , shandong agricultural university , taian 271018 , P.R.china) college of plant protection Shandong Agricultural University Taian 271018 P.R. China college of Life Sciences Shandong Agricultural University Taian 271018 P. R. China

The ultrastructural alteration of maize plants infected with the maize rough dwarf virus (MRDV) was studied with transmission electron microscopy. The results revealed that aggregates of virus particles , with a diameter of 60nm, were found in the root cell, and always distributed near the vacuole membrane. However, no such particles were checked in leaf cells. Moreover, no virus was observed in choroplasts, mitochondria nuclei, plasmodesmata or intercellular canal of all kinds of infected cells of maize, either. Structures of various organelles changed in the infected leaf and root cells of maize. An inward collapse and localized splitting of the tonoplast were observed, the chloropoast structure was destroyed by MRDV, and the number of destroyed or dysplasia chloroplast in leaf cells with serious symptoms was more than that in leaves without symptoms. The matrix of mitochondria in cells infected by MRDV decreased and some of them expanded and destructed. Nuclei was abnormal and the nuclear membrane was broken. In addition, the infected cells were characterized by a voluminous cytoplasm containing hypertrophied endoplasmic reticulum, with rich ribosome content and lots of starch grain.

关键词： Maize rough dwarf virus Cy topathology Electron microscopy

Effects of Synergism Between PVY and PVX on Virus Titer and Cell Ultrastructure in Tobacco plants

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agricultural Sciences in china 2003年第2期2卷 163-169,T001-T002页

作者： LI Han , GUO Xing-qi , LI Xiang-dong, GUO Heng-jun and LI Zhao-huicollege of Life Sciences , shandong agricultural university ,Tai’an 271018 , P. R. china college of plant protection , shandong agricultural university , Tai’an 271018 , P. R. china college of Life Sciences Shandong Agricultural University Tai'an 271018 P. R. China college of plant protection Shandong Agricultural University Tai'an 271018 P. R. China

The viruses titer and the ultrastructure of infected cells in tobacco host (Nicotiana tabacum cv. Samsun), which doubly infected with potato virus Y necrosis strain (PVYN) and potato virus X (PVX), were studied under greenhouse conditions. The results indicated that PVYN and PVX interacted synergistically. and tobacco plants which doubly infected with PVX and PVYN could greatly increase symptom severity as compared with that induced by the single virus. As determined by triple antibody sandwich enzyme-linked immunosor-bent assay (TAS-ELISA), the titer of PVX in the tobacco leaves infected with both PVYN and PVX was up to 9.10 times higher than the plants infected with PVX only. No significant differences in PVYN titer were detected between singly and doubly infected plants. The enhancement of PVX titer in doubly infected plants was evident in greenhouse and was independent of the virus strains, tested seasons, intervals between PVYN and PVX inoculation. When sections of doubly infected leaves were examined with an electron microscope, it could be frequently found that cells contained pinwheels and large masses of PVX-like particles, pinwheels and laminate inclusions, or all three structures, most of them were swollen, and their chloroplast and other organella were destructed heavily. This suggested that doubly infected cells were involved in the enhancement phenomenon, which seemed to be the result of an increase in the amount of PVX synthesized in them.

关键词： Potato virus Y Potato virus X Synergism Cytopathology

Effect of Different Fertilizing Methods on Zinc Nutrition in Zn-Deficiency Apple Trees

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agricultural Sciences in china 2002年第12期1卷 1365-1369页

作者： WANG Yan-an, FAN Wei-guo, ZHANG Fang-ai, LI Ling and LI De-quan(college of Science and Technology, shandong agricultural university, taian 271018 ,P. R.china college of Life Science , shandong agricultural university , taian 271018 , P. R. china) college of Science and Technology Shandong Agricultural University Taian 271018P.R.China college of Life Science Shandong Agricultural University Taian 271018 P.R.China

Red Fuji apple trees of Zn-deficiency were used as material in the experiment. The effect of different Zn application methods on the regularity of Zn transportation and nutrition was studied. It was found that the storage of Zn in the branches was greatly increased by foliar application of the solution of 15% ZnSO4·7H2O before abscission. It had advantage to keep suitable Zn content in leaves, branches and roots, as well as between Zn and P and K, which increased Zn transportation and reusability. Zn content in roots increased when Zn was used in soil in autumn. However, the absorbed Zn did not transport to the top of the plant. It was used by the plant next year. Foliar application of ZnSO4·7H2O at early stage of the plant growth enhanced Zn content of the branch. However, the effect was kept for a short time. It had little effect on Zn transportation when Zn was sprayed three weeks after blossoming.

关键词： Apple trees Zinc Nutrition balance Foliar application

Studies of induced colibacillosis in chickens

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Studies of induced colibacillosis in chickens

Invasive mechanism and control strategy of Ageratina adenophora (Sprengel)

The 15th World Veterinary Poultry Congress

作者： Cai Yumei~1 Chai Tongjie~1 Wang Xiaodong~2 1.college of Animal Science and Veterinary Medicine shandong agricultural university taian 271018 PR.china 2.college of plant protection shandong agricultural university taian 271018 PR.china

＜正＞Colibacillosis can be reproduced experimentally by inoculation of certain serogroups of Escherichia coli into ***,the severity of illness induced may vary,being influenced by many factors,one of which is the dos...

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来源： cnki会议评论

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Science china(Life Sciences) 2010年第11期53卷 1291-1298页

作者： WAN FangHao1, LIU WanXue1, GUO JianYing1, QIANG Sheng2, LI BaoPing2, WANG JinJun3, YANG GuoQing1, NIU HongBang1, GUI FuRong1, HUANG WenKun1, JIANG ZhiLin1 & WANG WenQi3 1State Key Laboratory for Biology of plant Diseases and Insect Pests, Institute of plant protection, Chinese Academy of agricultural Sciences, beijing 100193, china 2college of plant protection, Nanjing agricultural university, Nanjing 210095, china 3Key Laboratory of Entomology and Pest Control Engineering, college of plant protection, Southwest university, Chongqing 400716, china State Key Laboratory for Biology of plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences

In order to ascertain the invasive mechanism and control strategy of the invasive Crofton weed, Ageratina adenophora, its ecological adaptability and population differentiation,the formation of single dominant population, displacement of native plants and sustainable management strategies were investigated. The present results helped to clarify and explain such issues as the adaptability post invasion,interaction and competition between inter-and intra-species and community resistance, thereby providing important references to researches on other invasive alien species.

关键词： Ageratina adenophora invasive mechanism genetic diversity ecological adaptation allelopathy feedback of soil biota competitive displacement

Effects of Soil Aeration on Sweet Potato Yield and Its Physiological Mechanism

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agricultural Sciences in china 2002年第2期1卷 189-194页

作者： SHI Chun-yu,WANG Zhen-lin and YU Song-lie(college of Agronomy, shandong agricultural university, taian 271018) college of Agronomy Shandong Agricultural University Taian 271018

The effects of soil aeration on physiological characters and root tuber yield of Ipomoea batatas (L.) Lam. CV Lushu7 and Xushu18 were studied. The results showed that soil aeration improvement could increase ATP content and ATPase activity in functional leaves and root tubers and ABA content in root tubers. It also accelerated the transportation of 14C-photosynthate from leaves to root tubers and enhanced dry matter distribution in root tubers and thus root tuber yield was significantly raised. The role of ATP, ATPase and ABA in accelerating the transportation of 14C-photosynthate was discussed based on the changes of soluble carbonhydrate content in sweet potato plant.

关键词： Sweet potato Soil aeration Root tuber yield Physiological peculiarity

Ectopic expression of a hyacinth AGL6 homolog caused earlier flowering and homeotic conversion in Arabidopsis

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Science china(Life Sciences) 2007年第5期50卷 676-689页

作者： FAN JinHui1, LI WenQing2, DONG XiuChun1, GUO Wei1 & SHU HuaiRui3 1 college of Forestry, shandong agricultural university, taian 271018, china 2.college of Biological Science, china agricultural university, beijing 100094, china 3 college of Horticulture Science and Engineering, shandong agricultural university, taian 271018, china college of Forestry Shandong Agricultural University Taian 271018 China college of Biological Science China Agricultural University Beijing 100094 China college of Horticulture Science and Engineering Shandong Agricultural University Taian 271018 China

MADS-box genes are involved in floral organ *** we report thatan AGL6(Agamous-like 6)-like MADS-box gene,H0AGL6,was isolated from Hyacinthus *** pattern analysis demonstrated that H0AGL6 transcript was detected in inflorescencebuds,tepals,carpels and ovules,but not in stamina,leaves or *** Arabidopsis plantsectopically expressing H0AGL6 exhibited novel phenotypes of significantly reduced plantsize,extremely early flowering,and losing inflorescence *** addition,wide homeoticconversion of sepals,petals,and leaves into carpel-like or ovary structures,and disappearance ornumber reduction of stamens in 35S::HoAGL6 Arabidopsis plants were also ***-PCR analysisindicated that the expressions of flowering time gene SOC1 and flower meristem identity gene LFYwere significantly up-regulated in 35S::Ho4GL6transgenic Arabidopsis plants,and the expressionlevels of floral organ identity genes AG and SEP1 in leaves were also *** resultsindicated that H0AGL6 was involved in the regulation of flower transition and flower organformation.

关键词： AGL6 homologue, flower development, flowering time, MADS-box genes, Hyacinthus orientalis

Research on Indicator System of Regional Eco-efficiency:A Case Study of Chengyang District

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Chinese Journal of Population,Resources and Environment 2006年第4期4卷 54-58页

作者： Zhou Zhenfeng1,2,Sun Lei2,Sun Yinglan2 1.college of Resources&Environment,Laiyang agricultural college,Qingdao,shandong 266109,china 2.School of Environmental Science and Engineering,Ocean university of china,Qingdao,shandong 266003,china college of Resources & Environment Laiyang Agricultural College School of Environmental Science and Engineering Ocean University of China

Eco-efficiency is a valuable tool for managing and solving issues involving resource consumption and pollution emission in current production *** the popularity of the term"eco-efficiency"in business,limited attention has been paid to measuring and reporting regional eco-efficiency for local government policy *** on the concept and method of eco-efficiency,an indicator system of regional eco-efficiency is established in this *** indicator system comprises 22 indicators,which are divided in to three categories including socio-economic development,resources consumption, environmental *** a promising statistical technique, principle component analysis is used to set the weight of indicators which attempts to calculate the eco-efficiency indices of Qingdao's Chengyang *** results show that the eco-efficiency of Chengyang District has clearly improved 35.1% with small fluctuation from 1995 to ***-economic development index and resources consumption index also represent obvious increasing *** correlation coefficient between soci-economic development index and resources consumption index is 0.979,which means the social progress and economic growth of Chengyang District depend on an extravagant consumption of *** environmental pressure index increased slowly before 1997 and declined gradually after 1997,due to more attention being paid to environmental protection by local government in recent *** District still keeps the traditional economic development mode with a high consumption and high production,so the emphases of future development should put on improving the improving the efficient use of natural resources and promoting environmental management *** results show that the indicators system of regional eco-efficiency is a promising method to quantitatively evaluate resources and environmental efficiency and provide an effective decision-making support for local governments.

关键词： regional eco-efficiency indicator system Ecoefficiency Synthetic Index (ESI) Principle Component Analysis (PCA)

STUDIES ON THE RESISTANCE OF CAPSICUM VARIETIES TO BROAD MITE

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STUDIES ON THE RESISTANCE OF CAPSICUM VARIETIES TO BROAD MIT...